Strain

Ukraine_Kyiv_2014 (Region: Ukraine; Strain: ASFV/Kyiv/2016/131; Date: 2014)

Gene

NP1450L

Description

Annotated in NCBI, ASFV_Kyiv_2016_131_00159

Location

MN194591.1(-):133450-134196

GenBank Accession

QED21695.1

Full name

DNA-directed RNA polymerase subunit       + More
Probable DNA-directed RNA polymerase subunit 1 homolog
RPOLA_N domain-containing protein

CDS

ATGCGAAGACTTCCTCGCAAGGAGGGAAGAATTCGTAAATCCCTACTGGGCAGCCAGGTATGGTCGATCAGCCGGTCCACCATCTGCGGGAACTCAGACCTTCACCTGGACGAGGTTGGCTATCCCATTTCCTTTGCACGAACGCTGCAAGTTGCGGAAACAGTGCAACATTATAATATTAATAGATTAATGCCCTATTTTTTAAATGGAAAGCGCCAGTACCCTGGTTGTTCAAGAGTGTATAAGCAAATAACACAATCCGTTCACGACATTGAGGGTCTCAAACAAGACTTTAGGCTGGAGGTAGGAGACATCCTTTACCGTGACGTCGTCACTGGCGACGTCGCGTTTTTTAATCGCCAGCCTTCTCTCGAACGAAGCAGCATAGGGGTTCACCGGATTGTCGTTCTTGAAAACCCAAAAATTTCTACGTTTCAAATGAATGTCAGCGCATGTGCCTGGTATAATGCGGACTTTGACGGGGATCAGATGAATCTCTGGGTTCCCTGGAGCGTCATGAGCCGCGTTGAGGCCGAACTACTTTGTTCTGTGCGAAACTGGTTCATTTCCACAAAGAGCTCGGGTCCCGTTAATGGGCAGGTGCAGGACTCCACGGTGGGAAGCTTTTTGCTTACACGCACGAACACCCCCATGGGGAAAAATGTGATGAACAAGCTGCACGCCATGGGGTTGTTTCAAACAACCCAAACCGACCCACCTTGTTTTGCCAACTACTCCCCCAACTGA

Protein

MRRLPRKEGRIRKSLLGSQVWSISRSTICGNSDLHLDEVGYPISFARTLQVAETVQHYNINRLMPYFLNGKRQYPGCSRVYKQITQSVHDIEGLKQDFRLEVGDILYRDVVTGDVAFFNRQPSLERSSIGVHRIVVLENPKISTFQMNVSACAWYNADFDGDQMNLWVPWSVMSRVEAELLCSVRNWFISTKSSGPVNGQVQDSTVGSFLLTRTNTPMGKNVMNKLHAMGLFQTTQTDPPCFANYSPN

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)

Subunit

Component of the RNA polymerase that consists of at least 6 subunits.

Similarity

Belongs to the RNA polymerase beta' chain family.

Keywords

Complete proteome DNA-directed RNA polymerase Nucleotidyltransferase Transcription Transferase Reference proteome

Feature

chain Probable DNA-directed RNA polymerase subunit 1 homolog

Uniprot

A0A5B8XAD0 A0A0C5AYY7 A0A3S7XUW7 P0C987 A0A0C5AZF3 P0C989 + More
A9JL66 D4I5T6 A9JLZ8 A0A0A1E152 P0C988 E0WM49 A0A2X0RTZ9 P42486 Q65215 A0A1X6WFD9 A0A329RRR1 A0A080ZP32 W2PT81 W2KPH2 W2MTL0 Q0PPZ9 W2YSG5 I7A0B8 V9DV95 A0A1X7BZZ2 A0A0H3TMA5 A0A5C1DXQ7 A0A510P755 C3U089 A0A0P0ICQ6 A0A2V1AC29 A0A2P7YRM5 A0A421JI11 A0A077CZF3 B5RSM9 A0A0V1PWQ7 A0A141ZNE5 A0A1E4SJC8 A8VYZ6 A0A2U9GIY2 A0A4P9ZII1 A0A202GEZ4 A9Q9R1 A9Q9Q5 A0A1A0HAT4 A9Q9S2 A0A1L0C0C9 A0A512UPK2 A0A1E5RBH2 A9Q9T7 A9Q9N9 A0A1E4RGI8 A0A4P6XX01 B7SU40 A9Q9T5 A9Q9T4 A9Q9T9 A9Q9T6 A9Q9T8 G3AI95 G8XZU9 G8Y2U0 C4R4Y0 F2QW17 A9Q9N6 A0A1B2J8C6 A9Q9N8 A9Q9N4 A9Q9N3 A0A1X7QGV0 A0A1E5RSP7 A0A1E5RJ14 F7VUQ0 A9Q9N2 A0A1V0CNK9 A0A1L0CV49 F8N2G6 A0A0B0DHU4 Q7SDN0 G4U7S4 A9Q9N1 A0A2U9K7T9 A9Q9N5 A0A1B7TBJ4 W6MGR1 A0A1B2RUG8 B2AA31 A0A447BRT0 A0A077CVF5 S5CTB7 A0A1U9IN78 A0A175WB40 A0A1E3PBB3 A0A1U9IN92 A0A3N2PZR5 A9Q9L8 L2FJ99 A6MND8 A0A077CZ67

EC Number

2.7.7.6

Pubmed

25645905 30866784 30637968 18198370 25406173 26618713 + More
21470447 8506138 11831707 8021596 17051209 22978764 25878099 26903952 19287533 26474848 25132542 15229592 18024570 29786499 30297742 17981450 27856586 18945603 21788494 19465926 28412353 28775211 30287617 21575661 27388471 20386741 27801826 21750257 12712197 27535936 18460219 24558260 23818872 28298348 30368956 23252678 17486980

EMBL

MN194591 QED21695.1 KM111294 AJL34112.1 MK128995 MK333180 + More
MK333181 AYW34070.1 QBH90587.1 QBH90772.1 AY261363 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34277.1 AXB49327.1 AXB49501.1 AXB49673.1 AXB49844.1 AXB50017.1 AY261360 AM712239 CAN10198.1 FN557520 CBH29199.1 AM712240 CAN10448.1 KM262844 KM262845 KM102979 KP055815 KX354450 AIY22290.1 AIY22448.1 AJZ77039.1 AKO62780.1 AOO54449.1 AY261366 FR682468 CBW46765.1 MG939583 MG939588 LS478113 AXZ95873.1 AXZ96156.1 SPS73522.1 Z21489 U18466 AY261361 X71982 LT706986 SIP86012.1 MJFZ01000606 RAW26909.1 ANJA01002658 ETO68393.1 KI669605 ETN04168.1 KI681241 ETL86504.1 KI694488 ETM39671.1 DQ782853 ABG81916.1 ANIY01003014 ETP37603.1 JQ713084 AFO11473.1 ANIZ01004714 ETI29912.1 LT839607 SME65009.1 KJ614390 KU702948 KU702950 KU702952 AIB52040.1 AMN83263.1 AMN84247.1 AMN85234.1 CP043444 QEL61370.1 BGOX01000003 GBL51351.1 FJ358382 ACO89421.1 KT728691 ALK02055.1 PKFP01000003 PVH15498.1 PYFQ01000005 PSK38613.1 NSGQ01000068 RLV89627.1 KM065952 AIL23188.1 CR382133 CAR65414.1 LMYN01000080 KSA00614.1 KU702949 KU702951 KU556803 AMN83578.1 AMN84763.1 AMP44105.1 KV453911 ODV79542.1 EU186064 ABW38177.1 MH144201 AWQ64319.1 ML004432 RKP32272.1 LYUB02000001 OVF11231.1 EF689792 ABV69491.1 EF689786 ABV69485.1 LXTC01000003 OBA20992.1 EF689803 ABV69502.1 LT635768 SGZ56963.1 BIMT01000159 GEQ72972.1 LPNM01000008 OEJ84245.1 EF689818 ABV69517.1 EF689770 ABV69469.1 KV454542 ODV66384.1 CP034461 QBM90858.1 EU723816 ACI16423.1 EF689816 ABV69515.1 EF689815 ABV69514.1 EF689820 ABV69519.1 EF689817 ABV69516.1 EF689819 ABV69518.1 GL996500 EGW33664.1 FO082046 CCE87208.1 FO082047 CCE86101.1 FN392321 CAY70616.1 FR839630 CCA39595.1 EF689767 ABV69466.1 CP014584 ANZ74287.1 EF689769 ABV69468.1 EF689765 ABV69464.1 EF689764 ABV69463.1 CZDJ02000002 SMH63614.1 LPNL01000003 OEJ89932.1 LPNN01000005 OEJ86911.1 CABT02000008 CCC09246.1 EF689763 ABV69462.1 KX552040 ARA71945.1 FQNF01000010 SGZ38647.1 GL891382 EGO51638.1 KN389760 KHE80242.1 CM002236 EAA34861.2 GL890999 EGZ78364.1 EF689762 ABV69461.1 MH290814 AWS21920.1 EF689766 ABV69465.1 LXPE01000023 OBA26116.1 HG793125 CDK25021.1 KX090793 AOC37612.1 CU633438 FO904936 CAP59942.1 CDP22584.1 LR026964 VBB71595.1 KM065968 AIL23204.1 KF055414 AGQ04602.1 KX822561 APZ81068.1 LCTW02000067 KXX80164.1 KV454208 ODQ62252.1 KX822568 APZ81079.1 ML119053 ROT39993.1 EF689749 ABV69448.1 KB021047 ELA26479.1 EF413629 ABR31768.1 KM065956 AIL23192.1

Proteomes

UP000321214 UP000105860 UP000290386 UP000291821 UP000292678 UP000000859 + More
UP000101566 UP000000861 UP000130745 UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 UP000241813 UP000266411 UP000000858 UP000141072 UP000000624 UP000000860 UP000201921 UP000251314 UP000028582 UP000018817 UP000054423 UP000054532 UP000018948 UP000018721 UP000276409 UP000244303 UP000244713 UP000244774 UP000244833 UP000315293 UP000244406 UP000241107 UP000285775 UP000000599 UP000054251 UP000244556 UP000244783 UP000244814 UP000094285 UP000268321 UP000195602 UP000092555 UP000182259 UP000319698 UP000095728 UP000095085 UP000292447 UP000000709 UP000005222 UP000000314 UP000006853 UP000094565 UP000306804 UP000095605 UP000095358 UP000001881 UP000204182 UP000183365 UP000008065 UP000053122 UP000001805 UP000008513 UP000092321 UP000019384 UP000001197 UP000280685 UP000078237 UP000094112 UP000272025 UP000011096

PRIDE

P0C987 P0C989 P0C988 P42486 Q65215

Pfam

PF00623   RNA_pol_Rpb1_2
PF04998   RNA_pol_Rpb1_5
PF05000   RNA_pol_Rpb1_4
PF04997   RNA_pol_Rpb1_1
PF04990   RNA_pol_Rpb1_7
PF04983   RNA_pol_Rpb1_3
PF04992   RNA_pol_Rpb1_6
PF05001   RNA_pol_Rpb1_R

Interpro

IPR006592   RNA_pol_N
IPR000722   RNA_pol_asu
IPR007066   RNA_pol_Rpb1_3
IPR038593   RNA_pol_Rpb1_7_sf
IPR038120   Rpb1_funnel_sf
IPR007083   RNA_pol_Rpb1_4
IPR007081   RNA_pol_Rpb1_5
IPR007073   RNA_pol_Rpb1_7
IPR007080   RNA_pol_Rpb1_1
IPR042102   RNA_pol_Rpb1_3_sf
IPR007075   RNA_pol_Rpb1_6
IPR000684   RNA_pol_II_repeat_euk

Gene 3D

1.10.132.30 3.30.1360.140 1.10.274.100

ProteinModelPortal

A0A5B8XAD0 A0A0C5AYY7 A0A3S7XUW7 P0C987 A0A0C5AZF3 P0C989 + More
A9JL66 D4I5T6 A9JLZ8 A0A0A1E152 P0C988 E0WM49 A0A2X0RTZ9 P42486 Q65215 A0A1X6WFD9 A0A329RRR1 A0A080ZP32 W2PT81 W2KPH2 W2MTL0 Q0PPZ9 W2YSG5 I7A0B8 V9DV95 A0A1X7BZZ2 A0A0H3TMA5 A0A5C1DXQ7 A0A510P755 C3U089 A0A0P0ICQ6 A0A2V1AC29 A0A2P7YRM5 A0A421JI11 A0A077CZF3 B5RSM9 A0A0V1PWQ7 A0A141ZNE5 A0A1E4SJC8 A8VYZ6 A0A2U9GIY2 A0A4P9ZII1 A0A202GEZ4 A9Q9R1 A9Q9Q5 A0A1A0HAT4 A9Q9S2 A0A1L0C0C9 A0A512UPK2 A0A1E5RBH2 A9Q9T7 A9Q9N9 A0A1E4RGI8 A0A4P6XX01 B7SU40 A9Q9T5 A9Q9T4 A9Q9T9 A9Q9T6 A9Q9T8 G3AI95 G8XZU9 G8Y2U0 C4R4Y0 F2QW17 A9Q9N6 A0A1B2J8C6 A9Q9N8 A9Q9N4 A9Q9N3 A0A1X7QGV0 A0A1E5RSP7 A0A1E5RJ14 F7VUQ0 A9Q9N2 A0A1V0CNK9 A0A1L0CV49 F8N2G6 A0A0B0DHU4 Q7SDN0 G4U7S4 A9Q9N1 A0A2U9K7T9 A9Q9N5 A0A1B7TBJ4 W6MGR1 A0A1B2RUG8 B2AA31 A0A447BRT0 A0A077CVF5 S5CTB7 A0A1U9IN78 A0A175WB40 A0A1E3PBB3 A0A1U9IN92 A0A3N2PZR5 A9Q9L8 L2FJ99 A6MND8 A0A077CZ67

PDB

6J51 E-value=4.64905e-133, Score=1221

Ontologies

KEGG

vg:22220328

GO

GO:0006351  P:transcription, DNA-templated
GO:0003677  F:DNA binding
GO:0003899  F:DNA-directed 5'-3' RNA polymerase activity
GO:0039695  P:DNA-templated viral transcription
GO:0016021  C:integral component of membrane
GO:0006366  P:transcription by RNA polymerase II
GO:0005665  C:RNA polymerase II, core complex
GO:0000790  C:nuclear chromatin
GO:0046872  F:metal ion binding
GO:0010494  C:cytoplasmic stress granule
GO:0001055  F:RNA polymerase II activity
GO:0019985  P:translesion synthesis
GO:0003968  F:RNA-directed 5'-3' RNA polymerase activity

Subcellular Location

From MSLVP

Cytoplasm

Topology

Length:

248

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00147

Exp number, first 60 AAs:

0.00039

Total prob of N-in:

0.14885

outside

1 - 248

Population Genetic Test Statistics

Pi

0.0269762

Theta

0.0364084

Tajima's D

-0.787913

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62780.1
China_AnhuiXCGQ_2018	Protein	AYW34070.1
China_ASFV-SY18_2018	Protein	AKO62780.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95873.1
Poland_Pol16_20538_o9_2016-2017	Protein	CBW46765.1
Poland_Pol16_20540_o10_2016-2017	Protein	CBW46765.1
Poland_Pol16_29413_o23_2016-2017	Protein	CBW46765.1
Poland_Pol17_03029_C201_2016-2017	Protein	CBW46765.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96156.1
Poland_Pol17_05838_C220_2016-2017	Protein	CBW46765.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CBW46765.1
Uganda_R8_2015	Protein	AXB49327.1
Uganda_R7_2015	Protein	AXB49501.1
Uganda_R25_2015	Protein	AXB49673.1
Uganda_N10_2015	Protein	AXB49844.1
Uganda_R35_2015	Protein	AXB50017.1
Estonia_2014	Protein	SPS73522.1
Italy_26544_OG10_2010	Protein	AJZ77039.1
Italy_47_Ss_2008_2008	Protein	AOO54449.1
Portugal_OURT_88.3_1988	Protein	CAN10448.1
Spain_E75_1975	Protein	CBH29199.1
Russia_Georgia_2007	Protein Protein	CBW46765.1 CBW46765.1
Kenya_Tk1_2005	Protein	AJL34112.1
Kenya_Bus_2006	Protein	AJL34277.1
Russia_Odintsovo_2014	Protein	CBW46765.1
Portugal_L60_1960	Protein	AIY22290.1
Portugal_NHV_1968	Protein	AIY22448.1
Russia_Kashino_2013	Protein	CBW46765.1
Benin_1997	Protein	CAN10198.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CBW46765.1
South_Africa_MGR_Mkuzi_1979	Protein	CBW46765.1
Malawi_Lil_1983	Protein	CBW46765.1
Kenya_1950	Protein	CBW46765.1
Namibia_Warthog_1980	Protein	CBW46765.1
South_Africa_Warmbaths_1987	Protein	CBW46765.1
Malawi_Tengani_1962	Protein	CBW46765.1
China_HLJ_2018	Protein	QBH90587.1
China_LN_2018	Protein	QBH90772.1
China_wbBS01_2018	Protein	QDL88130.1
Ukraine_Kyiv_2014	Protein Protein Protein Protein	QED21693.1 QED21694.1 QED21695.1 QED21696.1
Belgium_Etalle_wb_2018	Protein	QED90542.1
Lithuania_LT14_2014	Protein	QEY87905.1
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence