Strain

Malawi_Tengani_1962 (Region: Malawi, Tengani, Nsanje District; Strain: Tengani 62; Date: 1962)

Gene

NP1450L

Description

Annoated by sequence mapping, NP1450L

Location

AY261364.1(-):124392-128744

GenBank Accession

CBW46765.1

Full name

DNA-directed RNA polymerase subunit

CDS

ATGGAGGCTGGATATGCCGAAATAGCCGCCGTTCAGTTCAATATTGCCGGTGACAATGATCATAAGAGGCAAGGCGTTATGGAGGTTACCATTTCCAATTTATTTGAGGGCACCCTTCCCGCCGAGGGGGGCATCTATGATGCACGAATGGGAACCACCGATCATCATTATAAATGCATCACTTGCTCACACCAGCGCAAGCAATGTATGGGACACCCTGGGATACTGCAGATGCATGCTCCGGTGCTTCAGCCGCTCTTCATCGCCGAAATACGACGATGGTTAAGGGTTATATGCCTCAACTGCGGGGCTCCCATCGTTGACCTAAAAAGATATGAGCACCTTATTAGGCCTAAGCGGCTTATTGAAGCCGCTTCAAGCCAGACTGAAGGAAAGCAGTGCTACGTCTGTAAGGCAGTACACCCCAAAATTATTAAGGACTCGGAAGATTATTTTACCTTTTGGGTAGATCAGCAGGGCAAGATTGACAAACTGTACCCGCAAATTATCAGAGAGATCTTTTCGCGCGTAACCTACGATACCGTTGTAAAACTGGGGCGAAGTAAAAACTCCCATCCCGAAAAACTTGTGCTTAAGGCCATTCAGATCCCCCCCATCAGCATACGACCTGGCATCAGATTGGGAATCGGGTCAGGCCCCCAAAGCTTTCACGACATTAACAACGTGATTCAGTATCTGGTTCGAAAGAATCTGCTGATCCCGAAGGACCTACAAATCGTGCGCGGCCAAAAAATACCTTTAAATATTGACCGCAATTTGCAAACCATACAGCAACTTTACTATAACTTTTTGTTGGATTCCGTTTCCACCACCGCAACCCAGGGAGGCACAGGAAAGCGTGGGATCGTCATGGGGGCCCGCCCCGCTCCCTCCATCATGCGAAGACTTCCTCGCAAGGAGGGAAGAATTCGCAAATCCCTACTGGGCAGCCAGGTATGGTCGATCAGCCGGTCCACCATCTGCGGGAACTCAGACTTTCACCTGGACGAGGTTGGCTATCCCATTTCCTTTGCACGAACGCTGCAAGTCGCGGAAACAGTGCAACATTATAATATTAATAGATTAATGCCCTATTTTTTAAATGGAAAGCGCCAGTACCCTGGTTGTTCAAGAGTGTATAAACAAATAACACAATCCGTTCACGACATTGAGGGCCTCAAACAAGACTTTAGACTGGAGGTAGGAGACATCCTTTACCGTGACGTCGTCACTGGCGACGTCGCGTTTTTTAATCGCCAGCCCTCTCTCGAACGAAGCAGCATAGGGGTTCACCGGATTGTCGTTCTTGAAAACCCAAAAATTTCTACGTTTCAAATGAATGTCAGCGCCTGTGCCTGGTATAATGCGGACTTTGATGGGGATCAGATGAATCTCTGGGTTCCCTGGAGCGTCATGAGCCGCGTCGAGGCCGAACTACTTTGTTCTGTGCGAAACTGGTTCATTTCCACGAAGAGCTCGGGTCCCGTTAATGGGCAGGTGCAGGACTCCACAGTGGGAAGCTTTTTGCTTACACGCACGAACACCCCCATGGGGAAAAATGTGATGAACAAGCTGCACGCCATGGGGTTGTTTCAAACAACCCAAACCGACCCACCTTGTTTTGCCAACTACTCCCCAACTGACCTGCTAGATGGCAAATCGGTTGTATCTATGCTACTGAAGCAGACCCCCATCAATTATCAACGAGCCCCCACATGGTACTCAGAAGTATATGCGCCCTACATGCATTATAATAAGCAGGACATCTCTACACAAATACGCAACGGTGAACTCATTGAAGGCGTCCTTGACAAAAAGGCCGTCGGAGCGGGCTCCTCCGGTGGAATCTATCACCTTATTTCGCGTAGGTATGGGCCGCAGCAGGCCTTGAAAATGATATTTGCGACCCAGCAGCTTGCTCTGAACTACGTGCGCAACGCCGGATTCACAGTGTCCACGGCCGACATGCTCTTAACCCCGGAAGCGCATCAGGAGGTCCAAGAAATTATCAATGAACTGCTGCTTGAGTCGGAGGAAATAAACAACCGGCTGCTTCATGGAGACATCATGCCGCCTATAGGCCTGACAACGCATGACTTCTACGAAAAATTACAGCTGAATGCGCTTAAATTTCCCGATAGGATTTTAAAGCCGATTATGAATTCCATTAATCCGGAAACCAATGGGCTTTTTCAGATGGTGGCCACTGGCGCCAAGGGCTCAAACCCCAATATGATTCACATCATGGCGGGCATCGGCCAAATTGAAATTAATACACAACGCATTCAACCCCAGTTTTCCTTCGGCAGGACCCTGGTGTACTACCCCAGGTTTGCCCTGGAGGCGCAGGCCTACGGGTTCATCTGCAACAGCTATATTGCGGGCCTTACCTCCCCTGAATTTATCTTTGGGGAAATGAATGGAAGATTCGACTTGATCAACAAAGCACTATCGACATCATCCACAGGCTATGCCAATCGTAAAGCCATCTTTGGCCTTCAATCCTGTATTGTGGATTATTACCGACGGGTTTCTATCGATACTCGTCTTGTGCAGCAGTTGTACGGAGAGGACGGCCTTGATGCGCGCCAGCTTGAAACCGTACGGTTTGAAACCATCATGCTGTCGGACCAGGAACTTGAAGACAAATTCAAGTACACCGGGATACAATCGCCCTTGTTTGAAGAAGAATTTTCACGCCTTAAAAAGGATAGAGATAAATATCGACAGATCTTCCTAAACGTCGAAAATTTTAATTTCAGTCAGCTGCTTACAGATGTTAGACAGGTGCCGGTAAACGTGGCCAGCATCGTAAAAAACATTCTACTGAGCTCCGCCAGTGGCGAGCTTCCCTTCGACGAAAAAAGTATTCTACAAAAATACACGATGGTCAAAACGTTTTGCAAAAATCTTCCATACGTGTTTATTAACAACATTCAGGAACGGCTACAAACGCCTATACCCGTTTATCTTAAACGGGCTGCCTCTCTGATGCGCATGCTTATTCGCATCGAACTGGCAACCGTTAAAACATTAAACATTACCTGCGAGCAGATGAGTGCCATCCTGGACCTCATAAGGCTACAATATACTCAAAGTCTTATTAACTACGGTGAGGCAGTGGGAATCCTGGCGGCGCAGTCCGTATCAGAGCCCTTGACTCAATACATGCTGGACTCCCACCACCGGTCCGTGGCTGGGGGAACCAACAAGTCGGGAATTGTGCGGCCCCAGGAGATCTTTAGCGCGAAGCCCGTAGAGGCTGAACAATCCTCTGAAATGCTTTTACGTCTAAAGAACCCGGAAGTGGAAACAAATAAAACATATGCGCAAGAAATTGCTAACAGCATAGAGCTTATAACGTTCGAACGGTTGATACTGCAGTGGCACCTATTGTACGAAACGTATTCAAGCACAAAAAAAAATGTGATGTACCCCGATTTTGCAAGTGATGTGGAATGGATGACGGATTTTCTGGAAAACCATCCTCTACTACAGCCCCCGGAGGATATTGCAAACTGGTGTATCCGCTTGGAATTAAACAAAACAACCATGATATTAAAAAGCATTAGTCTAGAAAGCATTATTAATAGTCTAAGAGCTAAACACCCCAACACCTATATCATGCATTCTGTGGAAAACACGGCCTCAGGTATTCCCATCATTATTCGCATATACCTACGGGAAAGCGCCTTTAGACGCAGTACCAATACCCGGATGGCTACGGATGAAAAAATTGCCGTAAATGTGGTGGATAAACTATTAAATAGCACGATTAGAGGAATACCAGGCATCAAAAATGCGAATGTTGTTAAGCTTATGCGCCACCGTGTGGATGCCCAGGGAAAGCTGGTAAGACTAGATAATATCTACGCCATCAAAACAAACGGGACTAATATTTTTGGCGCAATGCTTGATGATAACATCGACCCATACACCATCGTGTCGTCCTCCATAGGAGACACCATGGAGCTTTACGGCATAGAAGCGGCACGGCAAAAAATTATTAGTGAAATTAGAACGGTTATGGGGGACAAGGGTCCCAACCATCGTCATCTCCTCATGTACGCAGATCTCATGACAAGAACGGGACAGGTTACCTCTCTAGAAAAGGCAGGGCTTAATGCAAGAGAGCCTTCCAACGTGTTGTTGCGAATGGCCTTGTCGTCTCCAGTCCAGGTCTTGACGGATGCTGCGGTTGACTCCGCCGTGAATCCCATTTACGGTATTGCCGCGCCTACCCTTATGGGCTCTGTTCCTCGCATAGGGACCATGTACTCTGACATCATTATGGATGAAAAATATATTATGGAAAATTATAAAAGTGTTGATTCCATGATTGATATGTTATAG

Protein

MEAGYAEIAAVQFNIAGDNDHKRQGVMEVTISNLFEGTLPAEGGIYDARMGTTDHHYKCITCSHQRKQCMGHPGILQMHAPVLQPLFIAEIRRWLRVICLNCGAPIVDLKRYEHLIRPKRLIEAASSQTEGKQCYVCKAVHPKIIKDSEDYFTFWVDQQGKIDKLYPQIIREIFSRVTYDTVVKLGRSKNSHPEKLVLKAIQIPPISIRPGIRLGIGSGPQSFHDINNVIQYLVRKNLLIPKDLQIVRGQKIPLNIDRNLQTIQQLYYNFLLDSVSTTATQGGTGKRGIVMGARPAPSIMRRLPRKEGRIRKSLLGSQVWSISRSTICGNSDFHLDEVGYPISFARTLQVAETVQHYNINRLMPYFLNGKRQYPGCSRVYKQITQSVHDIEGLKQDFRLEVGDILYRDVVTGDVAFFNRQPSLERSSIGVHRIVVLENPKISTFQMNVSACAWYNADFDGDQMNLWVPWSVMSRVEAELLCSVRNWFISTKSSGPVNGQVQDSTVGSFLLTRTNTPMGKNVMNKLHAMGLFQTTQTDPPCFANYSPTDLLDGKSVVSMLLKQTPINYQRAPTWYSEVYAPYMHYNKQDISTQIRNGELIEGVLDKKAVGAGSSGGIYHLISRRYGPQQALKMIFATQQLALNYVRNAGFTVSTADMLLTPEAHQEVQEIINELLLESEEINNRLLHGDIMPPIGLTTHDFYEKLQLNALKFPDRILKPIMNSINPETNGLFQMVATGAKGSNPNMIHIMAGIGQIEINTQRIQPQFSFGRTLVYYPRFALEAQAYGFICNSYIAGLTSPEFIFGEMNGRFDLINKALSTSSTGYANRKAIFGLQSCIVDYYRRVSIDTRLVQQLYGEDGLDARQLETVRFETIMLSDQELEDKFKYTGIQSPLFEEEFSRLKKDRDKYRQIFLNVENFNFSQLLTDVRQVPVNVASIVKNILLSSASGELPFDEKSILQKYTMVKTFCKNLPYVFINNIQERLQTPIPVYLKRAASLMRMLIRIELATVKTLNITCEQMSAILDLIRLQYTQSLINYGEAVGILAAQSVSEPLTQYMLDSHHRSVAGGTNKSGIVRPQEIFSAKPVEAEQSSEMLLRLKNPEVETNKTYAQEIANSIELITFERLILQWHLLYETYSSTKKNVMYPDFASDVEWMTDFLENHPLLQPPEDIANWCIRLELNKTTMILKSISLESIINSLRAKHPNTYIMHSVENTASGIPIIIRIYLRESAFRRSTNTRMATDEKIAVNVVDKLLNSTIRGIPGIKNANVVKLMRHRVDAQGKLVRLDNIYAIKTNGTNIFGAMLDDNIDPYTIVSSSIGDTMELYGIEAARQKIISEIRTVMGDKGPNHRHLLMYADLMTRTGQVTSLEKAGLNAREPSNVLLRMALSSPVQVLTDAAVDSAVNPIYGIAAPTLMGSVPRIGTMYSDIIMDEKYIMENYKSVDSMIDML

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)

Similarity

Belongs to the RNA polymerase beta' chain family.

Uniprot

E0WM49 A0A2X0RTZ9 A0A2J7ZFT1 A0A2J8ADC3 A0A1V9XR03

EC Number

2.7.7.6

Pubmed

21470447 29294063 28327890

EMBL

FR682468 CBW46765.1 MG939583 MG939588 LS478113 AXZ95873.1 + More
AXZ96156.1 SPS73522.1 PGGS01004136 PNG99097.1 PGGS01000053 PNH10522.1 MNPL01005667 OQR75863.1

Proteomes

UP000141072 UP000192247

Pfam

PF05000   RNA_pol_Rpb1_4
PF04983   RNA_pol_Rpb1_3
PF04998   RNA_pol_Rpb1_5
PF00623   RNA_pol_Rpb1_2
PF04997   RNA_pol_Rpb1_1
PF04990   RNA_pol_Rpb1_7
PF04992   RNA_pol_Rpb1_6

Interpro

IPR007066   RNA_pol_Rpb1_3
IPR000722   RNA_pol_asu
IPR007081   RNA_pol_Rpb1_5
IPR038593   RNA_pol_Rpb1_7_sf
IPR038120   Rpb1_funnel_sf
IPR007083   RNA_pol_Rpb1_4
IPR007073   RNA_pol_Rpb1_7
IPR006592   RNA_pol_N
IPR007080   RNA_pol_Rpb1_1
IPR036844   Hint_dom_sf
IPR004042   Intein_endonuc
IPR007075   RNA_pol_Rpb1_6
IPR006141   Intein_N
IPR000684   RNA_pol_II_repeat_euk

SUPFAM

SSF51294 SSF51294

Gene 3D

1.10.132.30 3.30.1360.140

ProteinModelPortal

E0WM49 A0A2X0RTZ9 A0A2J7ZFT1 A0A2J8ADC3 A0A1V9XR03

PDB

6J51 E-value=4.64905e-133, Score=1221

Ontologies

GO

GO:0003899  F:DNA-directed 5'-3' RNA polymerase activity
GO:0003677  F:DNA binding
GO:0006351  P:transcription, DNA-templated
GO:0016539  P:intein-mediated protein splicing
GO:0004519  F:endonuclease activity
GO:0006366  P:transcription by RNA polymerase II
GO:0005665  C:RNA polymerase II, core complex

Subcellular Location

From MSLVP

Cytoplasm

Topology

Length:

1450

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.07482

Exp number, first 60 AAs:

0.00071

Total prob of N-in:

0.00043

outside

1 - 1450

Population Genetic Test Statistics

Pi

0.0280567

Theta

0.0375462

Tajima's D

-0.8600868

CLR

0.030234

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62780.1
China_AnhuiXCGQ_2018	Protein	AYW34070.1
China_ASFV-SY18_2018	Protein	AKO62780.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95873.1
Poland_Pol16_20538_o9_2016-2017	Protein	CBW46765.1
Poland_Pol16_20540_o10_2016-2017	Protein	CBW46765.1
Poland_Pol16_29413_o23_2016-2017	Protein	CBW46765.1
Poland_Pol17_03029_C201_2016-2017	Protein	CBW46765.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96156.1
Poland_Pol17_05838_C220_2016-2017	Protein	CBW46765.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CBW46765.1
Uganda_R8_2015	Protein	AXB49327.1
Uganda_R7_2015	Protein	AXB49501.1
Uganda_R25_2015	Protein	AXB49673.1
Uganda_N10_2015	Protein	AXB49844.1
Uganda_R35_2015	Protein	AXB50017.1
Estonia_2014	Protein	SPS73522.1
Italy_26544_OG10_2010	Protein	AJZ77039.1
Italy_47_Ss_2008_2008	Protein	AOO54449.1
Portugal_OURT_88.3_1988	Protein	CAN10448.1
Spain_E75_1975	Protein	CBH29199.1
Russia_Georgia_2007	Protein Protein	CBW46765.1 CBW46765.1
Kenya_Tk1_2005	Protein	AJL34112.1
Kenya_Bus_2006	Protein	AJL34277.1
Russia_Odintsovo_2014	Protein	CBW46765.1
Portugal_L60_1960	Protein	AIY22290.1
Portugal_NHV_1968	Protein	AIY22448.1
Russia_Kashino_2013	Protein	CBW46765.1
Benin_1997	Protein	CAN10198.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CBW46765.1
South_Africa_MGR_Mkuzi_1979	Protein	CBW46765.1
Malawi_Lil_1983	Protein	CBW46765.1
Kenya_1950	Protein	CBW46765.1
Namibia_Warthog_1980	Protein	CBW46765.1
South_Africa_Warmbaths_1987	Protein	CBW46765.1
Malawi_Tengani_1962	Protein	CBW46765.1
China_HLJ_2018	Protein	QBH90587.1
China_LN_2018	Protein	QBH90772.1
China_wbBS01_2018	Protein	QDL88130.1
Ukraine_Kyiv_2014	Protein Protein Protein Protein	QED21693.1 QED21694.1 QED21695.1 QED21696.1
Belgium_Etalle_wb_2018	Protein	QED90542.1
Lithuania_LT14_2014	Protein	QEY87905.1
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence