Strain

Ukraine_Kyiv_2014 (Region: Ukraine; Strain: ASFV/Kyiv/2016/131; Date: 2014)

Gene

NP1450L

Description

Annotated in NCBI, ASFV_Kyiv_2016_131_00158

Location

MN194591.1(-):131847-133421

GenBank Accession

QED21694.1

Full name

RPOLA_N domain-containing protein       + More
Probable DNA-directed RNA polymerase subunit 1 homolog
DNA-directed RNA polymerase subunit

CDS

ATGCTACTGAGGCAGACCCCCATCAATTATCAACGAGCCCCCACATGGTACTCAGAAGTGTATGCACCCTACATGCATTATAACAAGCAGGACATCTCTACACAAATACGCAACGGCGAACTCATTGAAGGCGTCCTTGACAAAAAGGCCGTCGGAGCGGGTTCCTCCGGTGGAATCTATCACCTTATTTCGCGTAGGTATGGGCCGCAGCAGGCCTTGAAAATGATATTTGCGACCCAGCAGCTCGCCCTAAACTACGTGCGCAACGCCGGATTCACAGTGTCCACGGCCGACATGCTCCTAACCCCGGAGGCACATCAGGAGGTCCAAGAAATTATCAATGAACTGCTGCTTGAGTCGGAGGAAATAAACAACCGGCTGCTTCATGGAGACATCATGCCGCCTATAGGCCTGACAACGCATGACTTCTACGAAAAATTGCAGCTGAATGCGCTTAAATTTCCCGATAGAATTTTAAAGCCGATTATGAATTCCATTAATCCGGAAACCAATGGGCTTTTTCAGATGGTGGCCACTGGCGCCAAGGGCTCAAACCCCAATATGATTCACATCATGGCGGGCATCGGCCAAATTGAAATTAATACACAACGCATTCAACCCCAGTTTTCCTTCGGTAGGACCCTGGTGTACTACCCCAGGTTTGCCCTGGAGGCGCAGGCCTACGGGTTCATCTGCAACAGCTATATTGCGGGCCTTACCTCCCCTGAATTTATCTTTGGGGAAATGAATGGAAGATTCGACTTGATCAACAAAGCATTATCGACATCATCCACAGGCTATGCCAACCGTAAAGCCATCTTTGGCCTTCAATCCTGTATTGTGGATTATTACCGACGGGTTTCCATCGATACGCGTCTTGTGCAGCAGTTGTACGGAGAGGACGGCCTTGATGCGCGCCAGCTTGAAACCGTACGGTTTGAAACCATCATGCTGTCGGACCAGGAACTTGAAGACAAATTCAAGTACACCGGGATACAATCGCCCTTGTTTGAAGAAGAATTTTCACGCCTTAAAAAGGATAGAGATAAATATCGACAGATCTTCCTAAACGTCGAAAATTTTAATTTCAGTCAGCTGCTTACAGATGTTAGACAGGTGCCGGTAAACGTGGCCAGCATCGTAAAAAACATTCTACTGAGCTCCACCAGTGGCGTGCTTCCCTTCGACGAAAAAAGTATTCTACAAAAATACGCGATGGTCAAAACGTTTTGCAAAAATCTTCCATACGTGTTTATTAACAACATTCAGGAACGACTACAAACGCCCATACCCGTTTATCTTAAACGGGCTGCCTCTCTGATGCGCATGCTTATTCGCATCGAACTGGCAACCGTTAAAACATTAAACATTACCTGCGAGCAGATGAGTGCCATCCTGGACCTCATAAGGCTACAATATACTCAAAGTCTTATTAACTACGGTGAGGCAGTGGGAATCCTGGCGGCGCAGTCCGTATCAGAGCCCTTGACACAATATATGCTGGACTCCCACCACCGGTCCGTGGCCGGGGGGAACCAACAAGTCGGGAATTGTGCGGCCCCAGGAGATCTTTAG

Protein

MLLRQTPINYQRAPTWYSEVYAPYMHYNKQDISTQIRNGELIEGVLDKKAVGAGSSGGIYHLISRRYGPQQALKMIFATQQLALNYVRNAGFTVSTADMLLTPEAHQEVQEIINELLLESEEINNRLLHGDIMPPIGLTTHDFYEKLQLNALKFPDRILKPIMNSINPETNGLFQMVATGAKGSNPNMIHIMAGIGQIEINTQRIQPQFSFGRTLVYYPRFALEAQAYGFICNSYIAGLTSPEFIFGEMNGRFDLINKALSTSSTGYANRKAIFGLQSCIVDYYRRVSIDTRLVQQLYGEDGLDARQLETVRFETIMLSDQELEDKFKYTGIQSPLFEEEFSRLKKDRDKYRQIFLNVENFNFSQLLTDVRQVPVNVASIVKNILLSSTSGVLPFDEKSILQKYAMVKTFCKNLPYVFINNIQERLQTPIPVYLKRAASLMRMLIRIELATVKTLNITCEQMSAILDLIRLQYTQSLINYGEAVGILAAQSVSEPLTQYMLDSHHRSVAGGNQQVGNCAAPGDL

Function

Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)

Subunit

Component of the RNA polymerase that consists of at least 6 subunits.

Similarity

Belongs to the RNA polymerase beta' chain family.

Keywords

Complete proteome DNA-directed RNA polymerase Nucleotidyltransferase Transcription Transferase Reference proteome

Feature

chain Probable DNA-directed RNA polymerase subunit 1 homolog

Uniprot

A0A5B8XAC5 A0A5B6TKV1 P0C988 P0C987 A0A3S7XUW7 E0WM49 + More
A0A2X0RTZ9 A0A0C5AZF3 P0C989 A9JL66 D4I5T6 A9JLZ8 A0A0A1E152 A0A0C5AYY7 Q65215 P42486 A0A1X6WFD9 A0A0H3TMA5 A0A1X7C074 A0A141ZNE4 A0A1X7QH14 A0A1V0CNM2 W2YSG5 A0A329RRR1 W2MTL0 W2KPH2 V9DV95 A0A5E4E5E6 M5VSC7 A0A5E4E3M6 A0A5E4E5J8 A0A4Y1RWD6 A0A498KG72 A0A2P6RLJ8 A0A2I0L133 A0A218W651 A0A540LH50 A0A196S9C9 A0A2J6L4J1 A0A0A0L655 A0A165Y5Y1 A0A1S3BJ90 A0A5D3CJC8 A0A540KB85 A0A5A7UGR7 A0A2U1MZE8 A0A061DF51 A0A061DGE8

EC Number

2.7.7.6

Pubmed

30866784 30637968 21470447 25645905 18198370 25406173 + More
26618713 8021596 8506138 11831707 25878099 26903952 27801826 23525075 31197015 28654223 28401891 19881527 19495411 20565788 22047402 26659563 22753475 29703587 23731509

EMBL

MN194591 QED21694.1 PXXM01000118 KAA3441418.1 AY261366 AY261363 + More
MK128995 MK333180 MK333181 AYW34070.1 QBH90587.1 QBH90772.1 FR682468 CBW46765.1 MG939583 MG939588 LS478113 AXZ95873.1 AXZ96156.1 SPS73522.1 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34277.1 AXB49327.1 AXB49501.1 AXB49673.1 AXB49844.1 AXB50017.1 AY261360 AM712239 CAN10198.1 FN557520 CBH29199.1 AM712240 CAN10448.1 KM262844 KM262845 KM102979 KP055815 KX354450 AIY22290.1 AIY22448.1 AJZ77039.1 AKO62780.1 AOO54449.1 KM111294 AJL34112.1 AY261361 X71982 Z21489 U18466 LT706986 SIP86012.1 KJ614390 KU702948 KU702950 KU702952 AIB52040.1 AMN83263.1 AMN84247.1 AMN85234.1 LT839607 SME65008.1 KU702949 KU702951 KU556803 AMN83579.1 AMN84762.1 AMP44106.1 CZDJ02000002 SMH63613.1 KX552040 ARA71948.1 ANIY01003014 ETP37603.1 MJFZ01000606 RAW26909.1 KI694488 ETM39671.1 KI681241 ETL86504.1 ANIZ01004714 ETI29912.1 CABIKO010000001 VVA09970.1 KB639097 CM007657 EMJ04257.1 ONH97196.1 VVA09972.1 VVA09971.1 AP019303 BBH08067.1 RDQH01000328 RXI06387.1 PDCK01000040 PRQ47312.1 PGOL01000208 PKI74424.1 MTKT01005034 OWM68245.1 VIEB01000587 TQD85770.1 LXWW01000486 OAO12961.1 NBSK01005116 PLY82164.1 CM002924 KGN55631.1 KV424810 KZV60964.1 SSTD01010811 TYK11392.1 VIEB01001543 TQD71496.1 SSTE01009989 KAA0052935.1 PKPP01003996 PWA66596.1 CM001879 EOX90744.1 EOX90741.1

Proteomes

UP000321214 UP000000858 UP000000859 UP000290386 UP000291821 UP000292678 + More
UP000141072 UP000101566 UP000000861 UP000130745 UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 UP000241813 UP000266411 UP000105860 UP000000860 UP000000624 UP000201921 UP000244303 UP000244713 UP000244774 UP000244833 UP000276409 UP000244556 UP000244783 UP000244814 UP000306804 UP000204182 UP000018948 UP000251314 UP000054532 UP000054423 UP000018721 UP000006882 UP000290289 UP000238479 UP000233551 UP000197138 UP000315295 UP000078348 UP000235145 UP000029981 UP000077086 UP000089565 UP000321947 UP000321393 UP000245207 UP000026915

PRIDE

P0C988 P0C987 P0C989 Q65215 P42486

Pfam

PF04998   RNA_pol_Rpb1_5
PF05000   RNA_pol_Rpb1_4
PF04983   RNA_pol_Rpb1_3
PF00623   RNA_pol_Rpb1_2
PF04997   RNA_pol_Rpb1_1
PF04990   RNA_pol_Rpb1_7
PF04992   RNA_pol_Rpb1_6
PF05001   RNA_pol_Rpb1_R

Interpro

IPR007081   RNA_pol_Rpb1_5
IPR038120   Rpb1_funnel_sf
IPR042102   RNA_pol_Rpb1_3_sf
IPR007083   RNA_pol_Rpb1_4
IPR007066   RNA_pol_Rpb1_3
IPR000722   RNA_pol_asu
IPR006592   RNA_pol_N
IPR038593   RNA_pol_Rpb1_7_sf
IPR007073   RNA_pol_Rpb1_7
IPR007080   RNA_pol_Rpb1_1
IPR007075   RNA_pol_Rpb1_6
IPR000684   RNA_pol_II_repeat_euk
IPR016181   Acyl_CoA_acyltransferase
IPR000182   GNAT_dom
IPR035698   RNAP_III_Rpc1_C
IPR035697   RNAP_III_RPC1_N

SUPFAM

SSF55729 SSF55729

Gene 3D

1.10.132.30 1.10.274.100 3.30.1360.140

CDD

cd02736 RNAP_III_Rpc1_C
cd02583 RNAP_III_RPC1_N

ProteinModelPortal

A0A5B8XAC5 A0A5B6TKV1 P0C988 P0C987 A0A3S7XUW7 E0WM49 + More
A0A2X0RTZ9 A0A0C5AZF3 P0C989 A9JL66 D4I5T6 A9JLZ8 A0A0A1E152 A0A0C5AYY7 Q65215 P42486 A0A1X6WFD9 A0A0H3TMA5 A0A1X7C074 A0A141ZNE4 A0A1X7QH14 A0A1V0CNM2 W2YSG5 A0A329RRR1 W2MTL0 W2KPH2 V9DV95 A0A5E4E5E6 M5VSC7 A0A5E4E3M6 A0A5E4E5J8 A0A4Y1RWD6 A0A498KG72 A0A2P6RLJ8 A0A2I0L133 A0A218W651 A0A540LH50 A0A196S9C9 A0A2J6L4J1 A0A0A0L655 A0A165Y5Y1 A0A1S3BJ90 A0A5D3CJC8 A0A540KB85 A0A5A7UGR7 A0A2U1MZE8 A0A061DF51 A0A061DGE8

PDB

6J51 E-value=4.64905e-133, Score=1221

Ontologies

KEGG

vg:22220328

GO

GO:0003677  F:DNA binding
GO:0006351  P:transcription, DNA-templated
GO:0003899  F:DNA-directed 5'-3' RNA polymerase activity
GO:0039695  P:DNA-templated viral transcription
GO:0006366  P:transcription by RNA polymerase II
GO:0005665  C:RNA polymerase II, core complex
GO:0016021  C:integral component of membrane
GO:0042797  P:tRNA transcription by RNA polymerase III
GO:0000790  C:nuclear chromatin
GO:0005666  C:RNA polymerase III complex
GO:0001056  F:RNA polymerase III activity
GO:0009506  C:plasmodesma
GO:0009507  C:chloroplast
GO:0005773  C:vacuole

Subcellular Location

From MSLVP

Cytoplasm

Topology

Length:

524

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.10295

Exp number, first 60 AAs:

0.00067

Total prob of N-in:

0.00530

outside

1 - 524

Population Genetic Test Statistics

Pi

0.0256294

Theta

0.0343035

Tajima's D

-0.917108

CLR

0.012508

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62780.1
China_AnhuiXCGQ_2018	Protein	AYW34070.1
China_ASFV-SY18_2018	Protein	AKO62780.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95873.1
Poland_Pol16_20538_o9_2016-2017	Protein	CBW46765.1
Poland_Pol16_20540_o10_2016-2017	Protein	CBW46765.1
Poland_Pol16_29413_o23_2016-2017	Protein	CBW46765.1
Poland_Pol17_03029_C201_2016-2017	Protein	CBW46765.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96156.1
Poland_Pol17_05838_C220_2016-2017	Protein	CBW46765.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CBW46765.1
Uganda_R8_2015	Protein	AXB49327.1
Uganda_R7_2015	Protein	AXB49501.1
Uganda_R25_2015	Protein	AXB49673.1
Uganda_N10_2015	Protein	AXB49844.1
Uganda_R35_2015	Protein	AXB50017.1
Estonia_2014	Protein	SPS73522.1
Italy_26544_OG10_2010	Protein	AJZ77039.1
Italy_47_Ss_2008_2008	Protein	AOO54449.1
Portugal_OURT_88.3_1988	Protein	CAN10448.1
Spain_E75_1975	Protein	CBH29199.1
Russia_Georgia_2007	Protein Protein	CBW46765.1 CBW46765.1
Kenya_Tk1_2005	Protein	AJL34112.1
Kenya_Bus_2006	Protein	AJL34277.1
Russia_Odintsovo_2014	Protein	CBW46765.1
Portugal_L60_1960	Protein	AIY22290.1
Portugal_NHV_1968	Protein	AIY22448.1
Russia_Kashino_2013	Protein	CBW46765.1
Benin_1997	Protein	CAN10198.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CBW46765.1
South_Africa_MGR_Mkuzi_1979	Protein	CBW46765.1
Malawi_Lil_1983	Protein	CBW46765.1
Kenya_1950	Protein	CBW46765.1
Namibia_Warthog_1980	Protein	CBW46765.1
South_Africa_Warmbaths_1987	Protein	CBW46765.1
Malawi_Tengani_1962	Protein	CBW46765.1
China_HLJ_2018	Protein	QBH90587.1
China_LN_2018	Protein	QBH90772.1
China_wbBS01_2018	Protein	QDL88130.1
Ukraine_Kyiv_2014	Protein Protein Protein Protein	QED21693.1 QED21694.1 QED21695.1 QED21696.1
Belgium_Etalle_wb_2018	Protein	QED90542.1
Lithuania_LT14_2014	Protein	QEY87905.1
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence