Strain

Ukraine_Kyiv_2014 (Region: Ukraine;  Strain: ASFV/Kyiv/2016/131;  Date: 2014)

Gene

G1211R

Description

Annotated in NCBI,  ASFV_Kyiv_2016_131_00145

Location

MN194591.1(+):115761-118208

GenBank Accession

QED21681.1

Full name

DNA polymerase       + More
DNA polymerase beta      
DNA_pol_B domain-containing protein      
DNA_pol_B_exo1 domain-containing protein      

CDS

ATGCAGCACGGTATTCTAGAAGAAATCTTTAACAAAATGAGCCTTTTCTGGCACCAAAAGCTGGATACCATTCTAAAATGCTATTACGTAAAGGAAAAGAGAGTCAAAATCTCGGCCGAAAAATCGATCATTTCCTCCTTTTTGCATACCCCTGGATGCCTACCCATTGATGTCCGCAACATGTGTATGCAGCTTTACCCTAAAGCCGAAAAAACAAGCTTGAAAGCGTTTTTAGAAAATTGTGGGTTAGATTCGAAGGTAGACCTGCCGTACCATCTCATGTGGAAGTATTATGAAACACGAGACAGCGAAAAAATAGCCGACGTGGCCTATTACTGCATTATAGATGCCCAGCGCTGTCAGGACCTTCTGGTGCGCCACAATGTTATCCCCGATCGCAGAGAGGTAGGAATTCTGTCATACACCTCGCTGTATGACTGTATCTACTACGCGGGAGGACACAAGGTATGCAATATGCTCATTGCCTATGCCATCCATGATGAATACGGCCGTATTGCTTGCAGTACCATTGCCCGAGGTAAGCGGGAACACGGAAAATATCCCGGCGCCTTTGTGATAGACCCCGTTAAAGGGCTTGAACAGGATAAACCCACCACAGGTCTCGACTTTGCGTCGCTGTACCCCTCACTCATCATGGCCTACAACTTTTCGCCAGAAAAATTTGTAGCCTCTCGGGATGAGGCAAATAGCCTCATGGCCAAGGGTGAATCTCTTCACTACGTCTCCTTTCACTTTAACAATCGTCTCGTGGAAGGATGGTTTGTGCGGCATAATAACGTTCCTGATAAAATGGGATTGTACCCAAAAGTACTCATCGATCTACTTAACAAACGGACCGCCCTTAAACAAGAGCTTAAAAAACTAGGTGAGAAAAAAGAATGTATCCATGAATCCCATCCTGGGTTTAAGGAACTACAGTTTCGCCATGCCATGGTAGACGCGAAGCAAAAGGCGTTGAAAATTTTCATGAACACGTTTTACGGCGAGGCAGGTAACAATTTGTCGCCCTTCTTTCTGCTTCCTCTAGCCGGAGGAGTCACCAGTTCGGGTCAATATAATCTTAAACTTGTCTATAACTTTGTTATCAATAAAGGTTACGGCATCAAGTACGGTGACACCGACTCATTATACATTACATGCCCAGATAGTCTTTATACAGAGGTAACAGACGCATATTTAAACAGCCAAAAAACGATAAAACATTATGAGCAACTCTGCCACGAAAAAGTGCTTCTGTCTATGAAAGCCATGTCTACACTATGCGCCGAGGTGAATGAATACCTGCGACAAGATAATGGCACCAGTTACCTACGTATGGCCTACGAGGAAGTACTCTTTCCTGTGTGCTTTACAGGCAAGAAAAAGTATTATGGTATTGCTCATGTAAACACACCCAATTTTAATACAAAAGAATTATTCATCCGCGGAATAGATATCATTAAGCAGGGTCAAACAAAACTCACCAAAACGATAGGAACGCGAATTATGGAAGAATCCATGAAACTACGCCGCCCTGAGGACCATCGCCCCCCTCTTATTGAAATCGTTAAAACGGTTTTGAAGGATGCTGTGGTTAACATGAAGCAGTGGAATTTTGAAGACTTCATCCAAACAGATGCGTGGAGACCGGACAAAGACAACAAAGCAGTCCAAATCTTTATGTCTCGCATGCACGCTCGGCGTGAGCAACTAAAAAAACACGGCGCTGCAGCATCGCAATTTGCTGAGCCCGAGCCGGGAGAACGCTTCTCCTACGTTATCGTGGAAAAACAGGTACAGTTTGATATCCAGGGCCACCGCACAGATTCCTCCAGAAAGGGGGACAAGATGGAATACGTCTCTGAAGCAAAGGCTAAAAATCTTCCTATTGATATATTGTTTTATATCAACAACTATGTTCTAGGCTTGTGCGCGAGATTCATTAATGAAAATGAAGAATTTCAACCCCCTGACAACGTCAGCAATAAGGATGAATACGCTCAGCGCCGAGCTAAATCCTACCTACAAAAATTCGTGCAATCCATTCACCCTAAAGACAAGTCTGTCATTAAGCAAGGCAATGTTCATCGACAGTGCTACAAATACATTCACCAAGAAATTAAAAAAAAAATAGGCATCTTTGCCGACCTTTATAAGGAATTTTTTAACAACACCACAAACCCCATCGAAAGCTTTATTCAAAGCACTCAGTTTATGATACAATACTTTGATGGAGAACAAAAAGTAAACCATTCTATGAAAAAAATGGTTGAACAGCATGCTACGGCTAGTAATCGAGCTGGTAAGCCCGCTGGTAATCCAGCCGGCAATGCGCTGATGCGGGCTATATTTACGCAGCTGATTACGGAAGAAAAAAAATTGTACAAGCCTTATACAATAAGGGGGATGCAATACACGATCTTCTCACCTATATCATTAACAATATAA

Protein

MQHGILEEIFNKMSLFWHQKLDTILKCYYVKEKRVKISAEKSIISSFLHTPGCLPIDVRNMCMQLYPKAEKTSLKAFLENCGLDSKVDLPYHLMWKYYETRDSEKIADVAYYCIIDAQRCQDLLVRHNVIPDRREVGILSYTSLYDCIYYAGGHKVCNMLIAYAIHDEYGRIACSTIARGKREHGKYPGAFVIDPVKGLEQDKPTTGLDFASLYPSLIMAYNFSPEKFVASRDEANSLMAKGESLHYVSFHFNNRLVEGWFVRHNNVPDKMGLYPKVLIDLLNKRTALKQELKKLGEKKECIHESHPGFKELQFRHAMVDAKQKALKIFMNTFYGEAGNNLSPFFLLPLAGGVTSSGQYNLKLVYNFVINKGYGIKYGDTDSLYITCPDSLYTEVTDAYLNSQKTIKHYEQLCHEKVLLSMKAMSTLCAEVNEYLRQDNGTSYLRMAYEEVLFPVCFTGKKKYYGIAHVNTPNFNTKELFIRGIDIIKQGQTKLTKTIGTRIMEESMKLRRPEDHRPPLIEIVKTVLKDAVVNMKQWNFEDFIQTDAWRPDKDNKAVQIFMSRMHARREQLKKHGAAASQFAEPEPGERFSYVIVEKQVQFDIQGHRTDSSRKGDKMEYVSEAKAKNLPIDILFYINNYVLGLCARFINENEEFQPPDNVSNKDEYAQRRAKSYLQKFVQSIHPKDKSVIKQGNVHRQCYKYIHQEIKKKIGIFADLYKEFFNNTTNPIESFIQSTQFMIQYFDGEQKVNHSMKKMVEQHATASNRAGKPAGNPAGNALMRAIFTQLITEEKKLYKPYTIRGMQYTIFSPISLTI

Function

DNA-directed DNA polymerase involved in viral DNA replication.

Catalytic Activity

a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)

Miscellaneous

Consistent with its intracellular location, ASFV encodes its own replicative DNA polymerase and three base excision repair enzymes: a class II AP endonuclease, the repair polymerase Pol X, and an ATP-dependent DNA ligase.

Similarity

Belongs to the DNA polymerase type-B family.

Keywords

Complete proteome   DNA replication   DNA-binding   DNA-directed DNA polymerase   Nucleotidyltransferase   Transferase   Viral DNA replication   Repeat   Reference proteome  

Feature

chain  DNA polymerase beta

Uniprot

A0A5B8XA87   D4I5S7   A9JLY2   A0A0A1E0S9   E0WM40   A0A2X0SE14   + More
P0C973   P0C971   P42489   A0A1C6ZXW4   A9JL52   A0A0C5BCR1   P43139   A0A0C5BC98   P0C972   P0C974   A0A397TXW1   A0A397GFA7   A0A397UYH4   A0A397IFZ4   A0A397IPN9   A0A397VQF2   A0A397JP61   A0A2N1MTD4   A0A2P4P297   A0A2H5RJC7   A0A015J5S9   A0A2N0NPC0   A0A2P4QNY4   A0A2P4PTY7   A0A2H5UD06   A0A2P4P5H3   A0A2H5T0A7   A0A2H5U2B9   A0A2H5U086   A0A2N0QVD4   A0A2N0QTH3   A0A2N0NPE5   A0A2N1M2D3   A0A2N1MBG7   A0A2N1MCP9   A0A2H5T0B7   A0A2P4QXU5   A0A397VDC9   A0A397V4U0   A0A397JEH9   A0A2Z6RQQ5   A0A2H5RLL7   A0A397IYT1   A0A2P4QP10   A0A372Q1S3   A0A397JBZ1   A0A2P4Q4H0  

EC Number

2.7.7.7

Pubmed

18198370    25406173    26618713    21470447    8293992    11831707    + More
8121806    25645905    8525640    24277808    29355972    30271968   

EMBL

MN194591    QED21681.1    FN557520    CBH29190.1    AM712240    CAN10439.1    + More
KM262844    KM262845    KP055815    KX354450    AIY22281.1    AIY22439.1    AKO62771.1    AOO54438.1    FR682468    CBW46756.1    MG939583    MG939587    MG939588    LS478113    AXZ95864.1    AXZ96052.1    AXZ96147.1    SPS73513.1    AY261366    AY261363    L19042    U18466    KM102979    AJZ77043.1    AM712239    CAN10189.1    KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    AJL34268.1    AXB49318.1    AXB49492.1    AXB49664.1    AXB49835.1    AXB50008.1    X73330    U27573    U27574    U27575    KM111294    AJL34103.1    AY261361    AY261360    QKWP01002964    RIB01728.1    PQFF01000454    RHZ49147.1    QKWP01000757    RIB15280.1    PQFF01000229    RHZ71983.1    PQFF01000158    RHZ77989.1    QKWP01000227    RIB24162.1    PQFF01000028    RHZ87756.1    LLXL01001358    PKK64906.1    AUPC02000447    POG59501.1    BDIQ01000048    AUPC02000458    GBC18196.1    POG59303.1    JEMT01030077    EXX50274.1    LLXJ01003907    PKB96421.1    AUPC02000025    POG79363.1    AUPC02000146    POG68847.1    BDIQ01000194    GBC52743.1    AUPC02000379    POG60624.1    BDIQ01000112    GBC35997.1    BDIQ01000165    GBC48976.1    BDIQ01000161    GBC48200.1    LLXH01002845    PKC55016.1    LLXH01003321    PKC54363.1    LLXJ01003885    PKB96451.1    LLXL01006953    LLXL01006853    PKK55728.1    PKK55773.1    LLXL01003272    PKK58991.1    LLXL01003056    PKK59422.1    BDIQ01000113    GBC36010.1    AUPC02000004    POG82469.1    QKWP01000440    RIB20012.1    QKWP01000598    RIB17554.1    PQFF01000043    RHZ86735.1    BEXD01003221    GBC00545.1    BDIQ01000050    GBC18994.1    PQFF01000115    RHZ81111.1    POG79362.1    QKKE01001491    RGB21943.1    PQFF01000074    RHZ84622.1    AUPC02000094    POG72530.1   

Proteomes

UP000321214    UP000101090    UP000108903    UP000110401    UP000142390    UP000241813    + More
UP000266411    UP000141072    UP000000858    UP000000859    UP000000624    UP000117635    UP000130745    UP000101566    UP000105860    UP000000860    UP000000861    UP000266673    UP000266861    UP000233469    UP000018888    UP000236242    UP000022910    UP000232722    UP000232688    UP000247702    UP000263633   

PRIDE

P0C971    P42489    P43139    P0C974   

Pfam

PF00136   DNA_pol_B
PF03104   DNA_pol_B_exo1

Interpro

IPR017964   DNA-dir_DNA_pol_B_CS
IPR042087   DNA_pol_B_C
IPR006172   DNA-dir_DNA_pol_B
IPR036397   RNaseH_sf
IPR012337   RNaseH-like_sf
IPR023211   DNA_pol_palm_dom_sf
IPR006134   DNA-dir_DNA_pol_B_multi_dom
IPR006133   DNA-dir_DNA_pol_B_exonuc

SUPFAM

SSF53098   SSF53098

Gene 3D

1.10.132.60     3.30.420.10     3.90.1600.10    

ProteinModelPortal

A0A5B8XA87   D4I5S7   A9JLY2   A0A0A1E0S9   E0WM40   A0A2X0SE14   + More
P0C973   P0C971   P42489   A0A1C6ZXW4   A9JL52   A0A0C5BCR1   P43139   A0A0C5BC98   P0C972   P0C974   A0A397TXW1   A0A397GFA7   A0A397UYH4   A0A397IFZ4   A0A397IPN9   A0A397VQF2   A0A397JP61   A0A2N1MTD4   A0A2P4P297   A0A2H5RJC7   A0A015J5S9   A0A2N0NPC0   A0A2P4QNY4   A0A2P4PTY7   A0A2H5UD06   A0A2P4P5H3   A0A2H5T0A7   A0A2H5U2B9   A0A2H5U086   A0A2N0QVD4   A0A2N0QTH3   A0A2N0NPE5   A0A2N1M2D3   A0A2N1MBG7   A0A2N1MCP9   A0A2H5T0B7   A0A2P4QXU5   A0A397VDC9   A0A397V4U0   A0A397JEH9   A0A2Z6RQQ5   A0A2H5RLL7   A0A397IYT1   A0A2P4QP10   A0A372Q1S3   A0A397JBZ1   A0A2P4Q4H0  

PDB

6TNZ     E-value=4.66016e-23,     Score=272