Strain

Uganda_N10_2015 (Region: Uganda, Tororo district;  Strain: N10;  Date: 2015)

Gene

pG1211R

Description

Annotated in NCBI,  aphidicolin-resistant

Location

MH025919.1(+):112191-115805

GenBank Accession

AXB49835.1

Full name

DNA polymerase beta       + More
DNA polymerase      

CDS

ATGGACCGTTCTGAGATTGTTGCACGGGAAAATCCGGTGATTACCCAACGGGTTACAAATCTCCTACGAACCAATGCTCCTCTACTATTCATGCCCATTGATATCCATGAAGTACGATATGGAGCCTACATGCTCTTCATGTATGGTTCCCTCGAAAACGGTTACAAAGCAGAAGTAAGGATTGAAAACATCCCAGTTTTCTTTGACGTACAGATTGAGTCTGATAACACAAATCAGCTTTTTTTAAAGTCGCTGCTGGCGGCTGAAAATATTACGTATGAACGGCTAGAGACGCTGACCCAGCGCCCAGTAATGGGGTATCGCGAGAAGGAAAAAGAGTTTGCGCCGTACATTCGAATATTTTTTAAAAGCTTATATGAGCGGCGAAAGGCGATTACTTACCTGAACAATATGGGTTACAACACGGCCGCGGACGACACAACCTGTTACTACCGAATGGTTTCCCGAGAGTTAAAACTGCCTCTTACAAGTTGGATACAGCTTCAGCAATATACCTATGAGCCTCGCGGCTTGGTACACAGGTTTTCAGTAACCCCTGAAGGTCTTGTTTCCTATCAGGATGATGTCCCCACAGACCACAGCATCGTAATGGCCTACGATATAGAAACCTATAGCCCTATTAAGGGAACCGTCCCGGACCCAAATCAGGCAAACGACGTGGTGTTCATGATATGCATGCGCATTTTTTGGATTCACTCCACAGAGCCTCTAGCAAGCACGTGCATCACCATGGCACCCTGTAAAAAATCCTCAGAGTGGACCACCATCGTATGTTCCTCTGAAAAAAATTTGCTGTTAAGCTTTGCTGAACAGTTTAGCCGCTGGGCTCCGGATATATGCACGGGGTTTAATGATTCTCGGTACGACTGGCCCTTTATCGTCGAAAAATCCATGCAGCACGGTATTCTCGAAGAAGTCTTTAACAAAATGAGCCTTTTCTGGCCCCAAAAGCTGGATACCATTCTAAAATGCTATTATGTGAAGGAAAAGAGAGTCAAAATCTCGGCCGAAAAATCTATCATTTCCTCCTTTTTGCATACCCCTGGATGCCTGCCCATTGATGTCCGCAACATGTGCATGCAGCTTTACCCTAAAGCCGAAAAAACAAGTCTAAAAGCGTTTTTAGAAAATTGTGGGCTAGATTCGAAGATAGACCTGCCGTACCATCTCATGTGGAAGTATTATGAAACGCGAGACAGTGAAAAGATGGCCGACGTGGCCTACTACTGCATTATAGATGCCCAGCGCTGTCAGGACCTTCTGGTGCGCCACAATGTTATCGCCGATCGCAGAGAGGTAGGAATCTTGTCATACACCTCGTTGTATGACTGTATCTACTACGCGGGAGGGCACAAGGTATGCAATATGCTTATTGCCTACGCCATCCATGATGAGTACGGCCGCATTGCCTGCAGCACCATTGCTCGGGGTAAGCGGGAACACGGAAAATATCCCGGCGCCTTTGTGATTGACCCCGTTAAAGGGCTTGAACAGGATAAACCCACCACCGGCCTCGACTTTGCGTCGCTATACCCCTCCCTCATCATGGCCTACAACTTTTCGCCAGAAAAATTTGTAGCCTCTCGGGATGAGGCAAATAGCCTCATAGCTAAGGGCGAGTCTCTTCACTACGTCTCCTTTCACTTCAATAACCGTCTAGTAGAAGGATGGTTTGTGCGGCATAATAACGTTCCTGATAAAATGGGATTGTACCCAAAAGTACTCATTGATCTACTTAATAAACGAACCGCTCTTAAACAAGAGCTTAAAAAACTAGGTGAAAAGAAGGAATGTATCCATGAATCCCATCCTGGGTTTAAGGAACTACAGTTCCGCCATGCCATGGTAGACGCGAAACAAAAGGCATTGAAAATTTTCATGAACACTTTTTACGGCGAGGCGGGTAACAATTTGTCACCCTTCTTTCTGCTTCCTCTGGCCGGAGGAGTCACCAGTTCGGGTCAATATAATCTTAAACTCGTCTATAACTTTGTTATCAACAAAGGTTACGGAATCAAGTACGGCGACACCGACTCATTATACATTACATGCCCGGATAGTCTTTATACAGAGGTAACAGACGCATACTTAAATAGTCAAAAAACAATAAAACATTATGAGCAACTCTGTCATGAAAAGGTGCTTCTGTCCATGAAGGCCATGTCTACACTATGCGCTGAGGTGAATGAATACCTGCGACAAGATAATGGTACCAGTTATCTACGTATGGCCTACGAGGAAGTACTCTTCCCCGTTTGCTTTACAGGCAAGAAAAAATATTACGGCATTGCTCATGTAAACACACCCAATTTTAATACAAAAGAATTATTCATCCGCGGAATAGATATCATTAAGCAGGGTCAAACAAAACTCACCAAAACGATAGGTACGCGAATTATGGAAGAATCCATGAAACTGCGCCGCCCTGAGGACCATCGCCCGCCTCTTATTGAAATCGTTAAAACGGTTTTAAAGGATGCTGTGGTTAACATGAAACAGTGGAATTTTGAAGACTTTATCCAAACAGATGCGTGGAGACCGGACAAAGACAACAAAGCAGTCCAAATCTTTATGTCTCGCATGCACGCTCGGCGTGAGCAACTAAAAAAACACGGCGCCGCCGCAACATCGCAATTTGCTGAGCCCGAGCCGGGAGAACGCTTCTCCTACGTTATCGTGGAAAAACAGGTGCAATTTGATATCCAAGGCCACCGCACAGACACCACCAGAAAGGGAGACAAAATGGAATACGTCTCTGAAGCAAAGGCTAAAAATCTTCCAATCGATATATTGTTTTATATCAATAACTATGTTCTAGGCCTGTGCGCGAGATTCATTAATGAAAATGAAGAGTTTCAGCCCCCCGGCAACGTCAGCAATAAGGACGAATACGCCCAGCGCCGAGCCAAATCCTACCTGCAAAAATTCGTGCAATCCATTCATCCTAAAGACAAGTCTGTCATTAAGCAAGGCATTGTTCATCGACAGTGCTACAAATACGTTCATCAAGAAATCAAAAAAAAAATAGGCATCTTTGCCGATCTTTATAAAGAATTTTTTAACAATACCACAAACCCCATCGAAAGCTTTATTCAAAGCACTCAGTTTATGATACATTACTTTGATGAAGAACAAAAAGTAAACCATTCAATGAAAAAAATGGTTGAGCAGCGTACTACTTTGGCTGGTAACCCGGCTGGTAACCCGGCTGGTAACCCAGCCAGCAATGCGCTGATGCGGGCTATATTTACGCAGTTGATTACGGAAGAAAAAAAAATTGTACAAGCCTTATACAATAAGGGGGACGCAATACACGACCTTCTCTCCTATATCATTAACAATATAAACTACAAAATTGCCACATTTCAGACGAAGCAGATGTTGACGTTCGAGCTTTCCAGTACTCATGTAGAACTGCTATTAAAACTAAACAAAACGTGGCTTATTTTGGTGGGAATTCATGTAGCAAAAAAACATCTGCATACTCTTTTGGGTTTATCTAACAATGAACCGCCGTCTAAGACATTCATTCAGCAAGCTATAGAGGAAGAATGTGGCAGTATTAAACCATCTTGCTACGACTTTATTTCCTAA

Protein

MDRSEIVARENPVITQRVTNLLRTNAPLLFMPIDIHEVRYGAYMLFMYGSLENGYKAEVRIENIPVFFDVQIESDNTNQLFLKSLLAAENITYERLETLTQRPVMGYREKEKEFAPYIRIFFKSLYERRKAITYLNNMGYNTAADDTTCYYRMVSRELKLPLTSWIQLQQYTYEPRGLVHRFSVTPEGLVSYQDDVPTDHSIVMAYDIETYSPIKGTVPDPNQANDVVFMICMRIFWIHSTEPLASTCITMAPCKKSSEWTTIVCSSEKNLLLSFAEQFSRWAPDICTGFNDSRYDWPFIVEKSMQHGILEEVFNKMSLFWPQKLDTILKCYYVKEKRVKISAEKSIISSFLHTPGCLPIDVRNMCMQLYPKAEKTSLKAFLENCGLDSKIDLPYHLMWKYYETRDSEKMADVAYYCIIDAQRCQDLLVRHNVIADRREVGILSYTSLYDCIYYAGGHKVCNMLIAYAIHDEYGRIACSTIARGKREHGKYPGAFVIDPVKGLEQDKPTTGLDFASLYPSLIMAYNFSPEKFVASRDEANSLIAKGESLHYVSFHFNNRLVEGWFVRHNNVPDKMGLYPKVLIDLLNKRTALKQELKKLGEKKECIHESHPGFKELQFRHAMVDAKQKALKIFMNTFYGEAGNNLSPFFLLPLAGGVTSSGQYNLKLVYNFVINKGYGIKYGDTDSLYITCPDSLYTEVTDAYLNSQKTIKHYEQLCHEKVLLSMKAMSTLCAEVNEYLRQDNGTSYLRMAYEEVLFPVCFTGKKKYYGIAHVNTPNFNTKELFIRGIDIIKQGQTKLTKTIGTRIMEESMKLRRPEDHRPPLIEIVKTVLKDAVVNMKQWNFEDFIQTDAWRPDKDNKAVQIFMSRMHARREQLKKHGAAATSQFAEPEPGERFSYVIVEKQVQFDIQGHRTDTTRKGDKMEYVSEAKAKNLPIDILFYINNYVLGLCARFINENEEFQPPGNVSNKDEYAQRRAKSYLQKFVQSIHPKDKSVIKQGIVHRQCYKYVHQEIKKKIGIFADLYKEFFNNTTNPIESFIQSTQFMIHYFDEEQKVNHSMKKMVEQRTTLAGNPAGNPAGNPASNALMRAIFTQLITEEKKIVQALYNKGDAIHDLLSYIINNINYKIATFQTKQMLTFELSSTHVELLLKLNKTWLILVGIHVAKKHLHTLLGLSNNEPPSKTFIQQAIEEECGSIKPSCYDFIS

Function

DNA-directed DNA polymerase involved in viral DNA replication.

Catalytic Activity

a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)

Miscellaneous

Consistent with its intracellular location, ASFV encodes its own replicative DNA polymerase and three base excision repair enzymes: a class II AP endonuclease, the repair polymerase Pol X, and an ATP-dependent DNA ligase.

Similarity

Belongs to the DNA polymerase type-B family.

Keywords

Complete proteome   DNA replication   DNA-binding   DNA-directed DNA polymerase   Nucleotidyltransferase   Repeat   Transferase   Viral DNA replication   Reference proteome  

Feature

chain  DNA polymerase beta

Uniprot

P0C971   A9JL52   A0A1C6ZXW4   P42489   P0C974   A0A0C5BCR1   + More
A0A0C5BC98   P0C972   P43139   P0C973   D4I5S7   A9JLY2   A0A0A1E0S9   E0WM40   A0A2X0SE14   A0A397IYT1   A0A397RZU3   A0A372QH90   A0A2I1FF43   A0A015LIA5   A0A397U3N3   A0A397JBZ1   A0A397JIU8   A0A2H5U9Y1   A0A015KK97   A0A397VBF3   A0A372RP47   A0A397TTP9   A0A397SAQ1   A0A2N0NSD9   A0A2Z6S9R4   A0A372Q7U9   A0A2I1HQM3   A0A2Z6Q746   A0A2H5RWE2   A0A2P4QBB7   A0A397WB07   A0A2Z6RJP1   A0A2H5SZE8   A0A015LZ95   A0A2P4QNY4   A0A397V2P0   A0A2I1GMI8   A0A397VLP2   A0A397UYH4   A0A397UIG3   A0A2Z6R6N7   A0A2N1MB26   A0A2I1FDZ4   A0A2I1F790   A0A397UWA3   A0A2N0QRA2   A0A2Z6QKX8   A0A2N0P367   A0A2Z6QNU7   A0A2N0REB3   A0A2Z6RET5   A0A2N1N7H0   A0A397SL57  

EC Number

2.7.7.7

Pubmed

18198370    8293992    11831707    8121806    25645905    8525640    + More
25406173    26618713    21470447    24277808    29355972    30271968   

EMBL

AY261363    AM712239    CAN10189.1    KM102979    AJZ77043.1    L19042    + More
U18466    AY261360    KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    AJL34268.1    AXB49318.1    AXB49492.1    AXB49664.1    AXB49835.1    AXB50008.1    KM111294    AJL34103.1    AY261361    X73330    U27573    U27574    U27575    AY261366    FN557520    CBH29190.1    AM712240    CAN10439.1    KM262844    KM262845    KP055815    KX354450    AIY22281.1    AIY22439.1    AKO62771.1    AOO54438.1    FR682468    CBW46756.1    MG939583    MG939587    MG939588    LS478113    AXZ95864.1    AXZ96052.1    AXZ96147.1    SPS73513.1    PQFF01000115    RHZ81111.1    QKYT01001161    RIA79750.1    QKKE01000594    RGB26687.1    LLXK01002766    PKY32984.1    JEMT01015443    EXX72396.1    QKWP01002329    RIB03758.1    PQFF01000074    RHZ84622.1    PQFF01000055    RHZ85906.1    BDIQ01000191    GBC51647.1    JEMT01026038    EXX60121.1    QKWP01000454    RIB19785.1    QKKE01000041    RGB41248.1    QKWP01003150    RIB01390.1    QKYT01000725    RIA81939.1    LLXJ01003171    PKB97499.1    BEXD01003502    GBC01347.1    QKKE01000878    RGB24093.1    LLXI01005036    PKY61189.1    BEXD01000217    GBB85385.1    BDIQ01000062    GBC22393.1    AUPC02000066    POG74918.1    QKWP01000028    RIB29843.1    BEXD01002443    GBB98304.1    BDIQ01000111    AUPC02000007    GBC35690.1    POG82023.1    JEMT01009706    JEMT01009705    EXX78060.1    EXX78061.1    AUPC02000025    POG79363.1    QKWP01000757    RIB15279.1    LLXI01000582    PKY47853.1    QKWP01000288    RIB22902.1    RIB15280.1    QKWP01001294    RIB10075.1    BEXD01001158    GBB92641.1    LLXL01003351    PKK58840.1    LLXK01002565    LLXK01002567    PKY32612.1    PKY32615.1    LLXH01000952    LLXK01001701    PKC61656.1    PKY30249.1    QKWP01001092    RIB11813.1    LLXH01004079    PKC53585.1    BEXD01000219    GBB85411.1    LLXJ01001653    PKC01275.1    BEXD01000935    GBB91215.1    LLXH01000955    PKC61639.1    BEXD01001864    GBB96059.1    LLXL01000689    PKK69789.1    QKYT01000562    RIA83541.1   

Proteomes

UP000000859    UP000130745    UP000117635    UP000000624    UP000000861    UP000101566    + More
UP000105860    UP000000860    UP000000858    UP000101090    UP000108903    UP000110401    UP000142390    UP000241813    UP000266411    UP000141072    UP000265703    UP000263633    UP000022910    UP000266673    UP000236242    UP000232722    UP000247702    UP000234323    UP000018888    UP000233469    UP000232688   

PRIDE

P0C971    P42489    P0C974    P43139   

Pfam

PF03104   DNA_pol_B_exo1
PF00136   DNA_pol_B

Interpro

IPR036397   RNaseH_sf
IPR006133   DNA-dir_DNA_pol_B_exonuc
IPR012337   RNaseH-like_sf
IPR017964   DNA-dir_DNA_pol_B_CS
IPR006172   DNA-dir_DNA_pol_B
IPR006134   DNA-dir_DNA_pol_B_multi_dom
IPR023211   DNA_pol_palm_dom_sf

SUPFAM

SSF53098   SSF53098

Gene 3D

3.30.420.10     3.90.1600.10    

ProteinModelPortal

P0C971   A9JL52   A0A1C6ZXW4   P42489   P0C974   A0A0C5BCR1   + More
A0A0C5BC98   P0C972   P43139   P0C973   D4I5S7   A9JLY2   A0A0A1E0S9   E0WM40   A0A2X0SE14   A0A397IYT1   A0A397RZU3   A0A372QH90   A0A2I1FF43   A0A015LIA5   A0A397U3N3   A0A397JBZ1   A0A397JIU8   A0A2H5U9Y1   A0A015KK97   A0A397VBF3   A0A372RP47   A0A397TTP9   A0A397SAQ1   A0A2N0NSD9   A0A2Z6S9R4   A0A372Q7U9   A0A2I1HQM3   A0A2Z6Q746   A0A2H5RWE2   A0A2P4QBB7   A0A397WB07   A0A2Z6RJP1   A0A2H5SZE8   A0A015LZ95   A0A2P4QNY4   A0A397V2P0   A0A2I1GMI8   A0A397VLP2   A0A397UYH4   A0A397UIG3   A0A2Z6R6N7   A0A2N1MB26   A0A2I1FDZ4   A0A2I1F790   A0A397UWA3   A0A2N0QRA2   A0A2Z6QKX8   A0A2N0P367   A0A2Z6QNU7   A0A2N0REB3   A0A2Z6RET5   A0A2N1N7H0   A0A397SL57  

PDB

6TNZ     E-value=4.66016e-23,     Score=272