Strain

Kenya_Tk1_2005 (Region: Kenya;  Strain: Ken05/Tk1;  Date: 2005)

Gene

BA71V-F778R

Description

Annotated in NCBI,  similar to African swine fever virus E75 strain E75 of INSD accession FN557520

Location

KM111294.1(+):57690-60029

GenBank Accession

AJL34059.1

Full name

Ribonucleoside-diphosphate reductase large subunit       + More
Ribonucleoside-diphosphate reductase      

Alternative Name

Ribonucleotide reductase large subunit

CDS

ATGGAGAACTTTTTTATTGTTAAGAAGTTGGCATCTGATACGTATGGAAAAGCGTTAAATGTTGATTTAGATAGACTATTACAAGCGCAGAATAAATATACTCTTCAAGAGCTTATTTCCTACTGCAGCGCTCTAACCATATTACATTATGACTATTCAACCCTTGCGGCACGTCTTTCGGTGTACCTACTGCATCAGTCAACGGCCTCCTCCTTCTCAGAAGCGGTAAGCCTACAGGCCGCACAATCCTGCTCACGCCTGTCTCCCCAGTTTGTGGACGTCGTCTACAAGTATAAAGCCATTTTTGACAGCTACATTGACTATAGCAGAGATTACAAGCTAACCCTCCTGGGGATAGAAACTATGAAAAATTCCTACTTGTTAAAAAATAAAGATGGCGTCATCATGGAACGCCCGCAGGACGCTTATATGCGCGTCGCCATTATGATTCATGGGATGGGAAGAGTGGTCAATATGAAGATGATTCTGCTGACCTATGACCTACTTTCCCGGCACGTCATCACACATGCATCGCCCACCATGTTCAATGCGGGCACCAAAAAGCCGCAGCTTTCCAGCTGCTTCCTGCTAAATGTAAACGATAATTTAGAAAATTTATACGATATGGTAAAAACGGCCGGCATCATTTCAGGCGGCGGCGGCGGAATAGGGCTGTGCTTGTCGGGAATACGGGCAAAAAATAGCTTTATCTCCGGTAGCGGTCTTAGAAGTAACGGCATACAAAATTATATTGTTTTACAGAATGCTTCACAATGCTACGCCAACCAGGGGGGCCTACGCCCCGGAGCCTACGCAGTCTACCTAGAGCTGTGGCATCAAGACATCTTTACATTTTTACAAATGCCCCGCCTAAAAGGACAAATGGCTGAACAGAGGCTTAATGCCCCCAATCTCAAGTACGGCCTATGGGTTCCCGACCTATTTATGGAAATACTCGAAGACCAAATACACGACAGAGGCGACGGCACATGGTACCTCTTTTCGCCGGATCAAGCCCCTAATCTACATAAGGTCTTTGATTTGGAACGATCGCGCCACAAAAATGCACATCGCGAATTTAGAAAGCTTTATTATCAGTATGTTGCCGAAAAGAGGTATACCGGCGTCACAACGGCCAAAGAGATTATCAAAGAGTGGTTTAAAACAGTCATTCAGGTGGGGAATCCCTATATCGGGTTTAAGGATGCGATCAATCGTAAAAGCAACCTTTCACACGTGGGCACCATCACTAACTCTAACCTCTGTATTGAGATCACAATTCCCTGCTGGGAGGGCAGTGAGGCCGAGCAGGGGGTTTGCAACCTTGCCGCGGTAAATCTGGCCGCCTTTATACGCGAAAACAGCTATGATTATCGTGGGCTCATAGAGGCAGCAGGCAACGTCACAGAAAATTTAGATAATATTATAGATAACGGCTACTACCCCACGGAGGCCACCCGTAGAAGCAATATGCGGCACCGACCCATTGGCATCGGGGTCTTTGGCCTGGCCGACGTGTTTGCGTCTTTTAAAATGAAATTTGGTTCACCCGAGGCCATTGCCATGGATGAGGCCATCCATGCAGCCCTATACTACGGCGCCATGCGACGATCCGTAGAACTTGCAAAAGAAAAAGGAAGTCACCCCAGTTTTCCGGGGTCTGCGGCCTCAAAAGGCCTGTTGCAACCCGACCTATGGGTTCGCTGCGATGATTTAGTTTTCTCCTGGGAAGAACGCGTGGCACAGACAACGCAGGGTGTGTTGACGCCAAAAAAGTGGTGGCAGCTACGGCTGGCGGCGATGCAGGGAGTCCGAAATGGATATCTCACGGCCCTTATGCCCACCGCAACCTCCTCAAATTCTACCGGCAAAAACGAATGCTTTGAGCCCTTTACATCCAATTTATATACACGTAGAACATTAAGCGGAGAATTTATTGTTTTAAATAAGTATTTAATAGATGATTTAAAAGAAATTAATCTTTGGACAGAGGCCATTCAACAGCAGCTACTAAATGCGGGGGGTAGCATTCAGCACATTCTGGATATACCGGCCGAGATCCGTGAACGATATAAAACCTCTAGAGAAATGAACCAGAAAATTTTAACAAAACACGCGGCCGCACGAAATCCCTTTGTGTCCCAAAGCATGTCTCTGAACTACTACTTCTATGAACCTGAATTAAGCCAAGTGCTTACAGTGCTCGTCCTAGGCTGGAAAAAGGGTCTAACCACCGGCTCCTATTACTGCCACTTTAGCCCCGGAGCGGGTACCCAAAAAAAGATTATAAGAAACTCTGAGAAAGCGTGTAGTGCGGACTGCGAGGCGTGTCTTCTGTAG

Protein

MENFFIVKKLASDTYGKALNVDLDRLLQAQNKYTLQELISYCSALTILHYDYSTLAARLSVYLLHQSTASSFSEAVSLQAAQSCSRLSPQFVDVVYKYKAIFDSYIDYSRDYKLTLLGIETMKNSYLLKNKDGVIMERPQDAYMRVAIMIHGMGRVVNMKMILLTYDLLSRHVITHASPTMFNAGTKKPQLSSCFLLNVNDNLENLYDMVKTAGIISGGGGGIGLCLSGIRAKNSFISGSGLRSNGIQNYIVLQNASQCYANQGGLRPGAYAVYLELWHQDIFTFLQMPRLKGQMAEQRLNAPNLKYGLWVPDLFMEILEDQIHDRGDGTWYLFSPDQAPNLHKVFDLERSRHKNAHREFRKLYYQYVAEKRYTGVTTAKEIIKEWFKTVIQVGNPYIGFKDAINRKSNLSHVGTITNSNLCIEITIPCWEGSEAEQGVCNLAAVNLAAFIRENSYDYRGLIEAAGNVTENLDNIIDNGYYPTEATRRSNMRHRPIGIGVFGLADVFASFKMKFGSPEAIAMDEAIHAALYYGAMRRSVELAKEKGSHPSFPGSAASKGLLQPDLWVRCDDLVFSWEERVAQTTQGVLTPKKWWQLRLAAMQGVRNGYLTALMPTATSSNSTGKNECFEPFTSNLYTRRTLSGEFIVLNKYLIDDLKEINLWTEAIQQQLLNAGGSIQHILDIPAEIRERYKTSREMNQKILTKHAAARNPFVSQSMSLNYYFYEPELSQVLTVLVLGWKKGLTTGSYYCHFSPGAGTQKKIIRNSEKACSADCEACLL

Function

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic Activity

[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate

Subunit

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).

Similarity

Belongs to the ribonucleoside diphosphate reductase large chain family.

Keywords

Allosteric enzyme   ATP-binding   Complete proteome   Disulfide bond   DNA replication   Early protein   Nucleotide-binding   Oxidoreductase   Reference proteome  

Feature

chain  Ribonucleoside-diphosphate reductase large subunit

Uniprot

P0C8H7   A0A0C5AWA1   P26685   A0A2Z5DF77   A0A0C5AWM9   E0WMI3   + More
A0A2X0RUV2   A0A1C6ZXX1   D4I5N3   A9JLP8   A0A0A1E309   P42491   A9JKY7   P0C8H8   P0C8H9   G0R8E6   A0A1G4M7R5   A0A1R2B7M2   A0A0N7MLU7   A0A1G4JNX9   C5DBR1   A0A1Q3ABM0   A0A1Q2ZX23   C5DTH2   G8ZZK3   A0A2C5ZK54   Q75AI4   A0A0C4DJZ8   R9XCL1   A0A1R2CM33   A0A2C5Y158   E1ZQX5   G8JTM8   C5KKC4   A0A0H2RSG9   A0A1S7HNH1   A0A1S7HB34   S6F3G8   A0A212M9A2   J3PBA8   Q6FPK3   A0A2T9YAG2   A0A0W0C8F4   A0A098VWL3   A0A0A8L4A3   L7I0W3   G4MNY7   L7JFA5   A0A010RVX7   A0A3N4L5K5   A0A2S4PM13   C5LWW8   A0A168B6W3   A0A0B1PD54   A0A067MEB0   A0A167TTD9  

EC Number

1.17.4.1

Pubmed

25645905    1871976    21470447    18198370    25406173    26618713    + More
11831707    18454138    19525356    22123960    15001715    23749448    20852019    23969048    15229592    29764946    22876203    15846337    30420746    27071652    25487071    24958869   

EMBL

AY261360    KM111294    AJL34059.1    M64728    MH025916    MH025917    + More
MH025918    MH025919    MH025920    AXB49274.1    AXB49448.1    AXB49620.1    AXB49791.1    AXB49964.1    KM111295    AJL34224.1    FR682468    CBW46711.1    MG939583    MG939587    MG939588    LS478113    AXZ95821.1    AXZ96009.1    AXZ96104.1    SPS73468.1    KM102979    KX354450    AJZ77051.1    AOO54375.1    FN557520    CBH29146.1    AM712240    CAN10393.1    KM262844    KM262845    KP055815    AIY22236.1    AIY22395.1    AKO62727.1    U18466    AM712239    CAN10144.1    AY261363    AY261366    GL985056    EGR52336.1    LT598489    SCV99886.1    MPUH01000876    OMJ72746.1    LN890527    CUS23283.1    LT598465    SCU92427.1    CU928165    CAR21218.1    BDGX01000035    GAV53072.1    BDGX01000009    GAV48060.1    CU928175    CAR27083.1    HE616749    CCE94047.1    NJEU01000175    PHH79774.1    AE016817    AAS51863.2    ADBL01000012    GL876966    KLU80963.1    CP006023    AGO11716.1    MPUH01000111    OMJ90074.1    NJET01000122    PHH60952.1    GL433860    EFN51865.1    CP002500    AET39381.1    GG673688    EER15036.1    KQ085938    KLO14804.1    CP019496    AQZ14496.1    CP019491    AQZ10224.1    HG316457    CDF89382.1    FUGC01000008    SJM85780.1    GL385400    EJT71524.1    CR380956    CAG60790.1    MBFR01000323    PVU89321.1    LLZZ01000178    KTA95909.1    JMKJ01000113    KGG52146.1    CCBQ010000019    CDO93078.1    JH793404    ELQ36621.1    CM001231    EHA57144.1    JH795555    ELQ66519.1    JARH01000148    EXF84721.1    ML119107    RPB17058.1    PEDP01001935    POS83057.1    GG686286    EEQ98746.1    AZHB01000005    OAA69705.1    JNVN01000643    KHJ34846.1    KL198073    KDQ09906.1    AZHD01000008    OAA60927.1   

Proteomes

UP000000861    UP000105860    UP000101566    UP000141072    UP000117635    UP000266411    + More
UP000101090    UP000108903    UP000110401    UP000142390    UP000241813    UP000000624    UP000130745    UP000000859    UP000000858    UP000008984    UP000190831    UP000236544    UP000191024    UP000002036    UP000187013    UP000008536    UP000005627    UP000224854    UP000000591    UP000011715    UP000016920    UP000226192    UP000008141    UP000006790    UP000053477    UP000190375    UP000019375    UP000196771    UP000006039    UP000002428    UP000054886    UP000031516    UP000011086    UP000009058    UP000011085    UP000020467    UP000076744    UP000027195    UP000076874   

PRIDE

P0C8H7    P26685    P42491   

Pfam

PF02867   Ribonuc_red_lgC
PF00317   Ribonuc_red_lgN
PF03477   ATP-cone

Interpro

IPR013346   NrdE_NrdA
IPR039718   Rrm1
IPR013509   RNR_lsu_N
IPR000788   RNR_lg_C
IPR008926   RNR_R1-su_N
IPR005144   ATP-cone_dom
IPR036322   WD40_repeat_dom_sf
IPR015943   WD40/YVTN_repeat-like_dom_sf
IPR001680   WD40_repeat

SUPFAM

SSF48168   SSF48168
SSF50978   SSF50978

Gene 3D

2.130.10.10    

ProteinModelPortal

P0C8H7   A0A0C5AWA1   P26685   A0A2Z5DF77   A0A0C5AWM9   E0WMI3   + More
A0A2X0RUV2   A0A1C6ZXX1   D4I5N3   A9JLP8   A0A0A1E309   P42491   A9JKY7   P0C8H8   P0C8H9   G0R8E6   A0A1G4M7R5   A0A1R2B7M2   A0A0N7MLU7   A0A1G4JNX9   C5DBR1   A0A1Q3ABM0   A0A1Q2ZX23   C5DTH2   G8ZZK3   A0A2C5ZK54   Q75AI4   A0A0C4DJZ8   R9XCL1   A0A1R2CM33   A0A2C5Y158   E1ZQX5   G8JTM8   C5KKC4   A0A0H2RSG9   A0A1S7HNH1   A0A1S7HB34   S6F3G8   A0A212M9A2   J3PBA8   Q6FPK3   A0A2T9YAG2   A0A0W0C8F4   A0A098VWL3   A0A0A8L4A3   L7I0W3   G4MNY7   L7JFA5   A0A010RVX7   A0A3N4L5K5   A0A2S4PM13   C5LWW8   A0A168B6W3   A0A0B1PD54   A0A067MEB0   A0A167TTD9  

PANTHER

PTHR11573  

PDB

3S8B     E-value=4.81754e-140,     Score=1279