Strain

Italy_26544_OG10_2010 (Region: Sardinian,Italy; Strain: 26544/OG10; Date: 2010)

Gene

P1192R

Description

Annotated in NCBI, gene expression: late

Location

KM102979.1(+):141915-145493

GenBank Accession

AJZ77044.1

Full name

DNA topoisomerase 2

Alternative Name

DNA topoisomerase II

CDS

ATGGAAGCGTTTGAAATCAGCGATTTCAAAGAGCATGCGAAGAAAAAAAGCATGTGGGCTGGCGCCCTCAACAAAGTCACTATTTCGGGTCTTATGGGGGTCTTTACCGAAGATGAGGACCTTATGGCGTTACCCATTCACAGAGACCACTGTCCCGCTTTGTTAAAAATTTTTGACGAGCTCATCGTAAATGCCACGGATCATGAAAGAGCTTGCCATAGCAAAACAAAAAAGGTAACCTACATCAAAATTTCGTTTGATAAAGGCGTGTTTTCTTGCGAAAACGATGGCCCGGGAATCCCCATTGCAAAGCATGAGCAGGCCAGTCTTATCGCCAAGCGCGATGTGTATGTTCCCGAGGTGGCTTCATGCTTCTTTCTAGCCGGAACGAACATCAATAAGGCCAAGGACTGTATCAAGGGGGGAACCAACGGCGTCGGGCTGAAGCTCGCCATGGTGCATTCGCAGTGGGCCATTCTTACCACCGCCGACGGCGCGCAAAAGTATGTTCAACAAATCAACCAGCGCCTAGATATCATTGAGCCTCCTACCATTACACCCTCCAGGGAAATGTTTACACGTATCGAGCTCATGCCCGTATACCAGGAACTAGGGTACGCGGAGCCTCTGTCTGAAACGGAGCAAGCAGATCTTTCCGCCTGGATTTATCTTCGCGCCTGCCAATGCGCGGCCTACGTGGGAAAAGGCACCACCATTTATTACAATGATAAGCCTTGCCGCACGGGCTCTGTGATGGCGCTGGCCAAAATGTACACCCTGTTGAGCGCGCCTAATAGCACGATACATACGGCGACCATTAAGGCCGACGCAAAACCCTATAGCCTGCACCCTCTGCAGGTTGCGGCGGTCGTGTCCCCCAAGTTTAAAAAATTTGAACACGTGTCCATTATCAACGGGGTAAATTGTGTAAAAGGAGAACATGTTACCTTTTTGAAAAAGACCATTAATGAAATGGTCATTAAAAAATTTCAACAGACGATTAAAGATAAAAACCGCAAAACAACATTACGTGACAGCTGTTCAAACATCTTTGTCGTTATAGTGGGTTCCATTCCAGGCATAGAATGGACCGGCCAGCGGAAGGATGAACTTAGCATCGCAGAAAATGTTTTTAAAACGCATTACTCCATCCCTTCTAGTTTTTTAACAAGCATGACAAGGTCTATCGTGGATATTCTTCTGCAATCCATTTCTAAAAAAGATAACCATAAACAGGTCGACGTAGACAAATATACGCGTGCCCGCAATGCGGGAGGGAAAAGGGCGCAGGACTGCATGCTACTCGCGGCGGAAGGGGATAGCGCACTTTCCCTGTTGCGCACGGGACTGACCCTGGGAAAGTCCAACCCAAGCGGGCCCTCCTTTGACTTCTGCGGCATGATCTCCCTGGGAGGGGTCATCATGAATGCCTGCAAAAAGGTGACAAACATTACAACGGACTCTGGAGAAACCATCATGGTGCGCAACGAACAGCTTACCAATAATAAAGTGTTGCAGGGAATTGTGCAGGTATTGGGTCTAGACTTCAACTGCCATTACAAAACGCAGGAAGAGCGAGCAAAGCTGAGATACGGCTGCATTGTTGCGTGCGTTGATCAAGATCTGGATGGGTGTGGAAAAATCCTTGGACTGCTGCTGGCCTACTTTCACCTGTTTTGGCCTCAGCTTATTATCCATGGTTTCGTAAAACGACTGCTTACCCCGCTGATACGTGTGTACGAAAAGGGCAAGACTATGCCCGTAGAATTTTACTATGAACAGGAGTTTGATGCCTGGGCAAAAAAGCAGACCAGCTTAGTCAATCATACTGTAAAATATTACAAGGGATTGGCGGCGCATGACACCCATGAAGTAAAAAGCATGTTCAAACATTTTGACAACATGGTGTACACGTTTACCCTGGATGACTCGGCAAAGGAGTTGTTTCATATTTATTTTGGCGGGGAGTCGGAGTTGCGAAAAAGAGAGCTTTGCACCGGCGTGGTGCCGCTCACTGAAACCCAGACGCAGTCCATTCATAGTGTCCGACGAATTCCTTGCAGCCTGCATCTGCAGGTAGATACCAAGGCTTACAAGCTGGATGCCATCGAGCGGCAGATTCCCAACTTCTTAGACGGAATGACGCGGGCGCGGCGCAAAATTTTAGCCGGGGGGGTGAAATGCTTCGCTTCCAACAACCGTGAACGAAAGGTTTTTCAGTTCGGGGGCTACGTTGCGGATCACATGTTTTATCACCATGGCGACATGTCGTTAAACACAAGTATTATAAAAGCCGCCCAGTATTACCCGGGCTCCTCCCACCTCTATCCAGTATTCATAGGCATAGGAAGCTTCGGCTCCAGGCACCTGGGAGGAAAGGATGCAGGATCCCCAAGATACATCAGTGTGCAGCTTGCGTCTGAATTTATTAAAACAATGTTCCCCGCGGAGGACTCATGGCTTCTCCCCTACGTCTTTGAGGACGGCCAGCGGGCGGAACCAGAGTACTACGTGCCTGTATTGCCGCTTGCTATTATGGAGTACGGCGCCAACCCATCGGAGGGCTGGAAGTACACCACTTGGGCCCGGCAACTGGAAGACATTTTGGCCTTGGTGAGGGCCTACGTCGACAAAGACAACCCAAAACACGAGCTACTGCACTATGCAATAAAACATAAGATTACTATACTCCCGCTGCGGCCCTCCAATTACAATTTCAAGGGCCATTTGAAGCGGTTTGGCCAATACTACTACAGCTACGGCACGTACGACATCTCAGAGCAGCGAAATATAATTACTATTACGGAGCTTCCTCTGCGTGTTCCTACGGTTGCATATATCGAAAGTATAAAAAAATCGAGTAACCGCATGACATTTATTGAAGAAATCATCGACTACAGTAGTTCAGAAACCATTGAAATTCTGGTGAAACTAAAGCCAAATAGTCTCAACCGTATCGTGGAAGAATTTAAGGAGACTGAAGAGCAAGATTCCATAGAAAATTTTCTGCGCCTGCGCAATTGTTTACATTCGCATCTAAACTTTGTAAAACCTAAAGGTGGTATTATCGAGTTTAACTCATATTATGAAATTTTATATGCGTGGCTACCTTACAGGCGTGAGCTTTACCAAAAGCGTCTTATGCGTGAGCACGCGGTGCTTAAGCTGCGCATTATCATGGAAACTGCTATTGTACGCTACATCAATGAGTCTGCAGAGCTAAATCTTTCCCATTATGAGGATGAAAAGGAGGCAAGCCGCATTCTAAGCGAGCATGGATTTCCCCCGCTGAACCACACGCTGATCATTTCCCCTGAGTTTGCCTCTATAGAGGAACTCAATCAAAAAGCGCTGCAGGGCTGTTATACCTATATACTATCTTTGCAGGCTCGAGAATTGCTTATCGCAGCCAAAACTCGTCGGGTGGAAAAAATAAAAAAAATGCAAGCTCGTCTTGATAAGGTTGAGCAGCTTTTGCAGGAGTCTCCCTTTCCCGGCGCCAGCGTATGGCTGGAGGAAATTGATGCGGTGGAAAAGGCTATTATAAAAGGAAGAAATACTCAGTGGAAATTTCATTAA

Protein

MEAFEISDFKEHAKKKSMWAGALNKVTISGLMGVFTEDEDLMALPIHRDHCPALLKIFDELIVNATDHERACHSKTKKVTYIKISFDKGVFSCENDGPGIPIAKHEQASLIAKRDVYVPEVASCFFLAGTNINKAKDCIKGGTNGVGLKLAMVHSQWAILTTADGAQKYVQQINQRLDIIEPPTITPSREMFTRIELMPVYQELGYAEPLSETEQADLSAWIYLRACQCAAYVGKGTTIYYNDKPCRTGSVMALAKMYTLLSAPNSTIHTATIKADAKPYSLHPLQVAAVVSPKFKKFEHVSIINGVNCVKGEHVTFLKKTINEMVIKKFQQTIKDKNRKTTLRDSCSNIFVVIVGSIPGIEWTGQRKDELSIAENVFKTHYSIPSSFLTSMTRSIVDILLQSISKKDNHKQVDVDKYTRARNAGGKRAQDCMLLAAEGDSALSLLRTGLTLGKSNPSGPSFDFCGMISLGGVIMNACKKVTNITTDSGETIMVRNEQLTNNKVLQGIVQVLGLDFNCHYKTQEERAKLRYGCIVACVDQDLDGCGKILGLLLAYFHLFWPQLIIHGFVKRLLTPLIRVYEKGKTMPVEFYYEQEFDAWAKKQTSLVNHTVKYYKGLAAHDTHEVKSMFKHFDNMVYTFTLDDSAKELFHIYFGGESELRKRELCTGVVPLTETQTQSIHSVRRIPCSLHLQVDTKAYKLDAIERQIPNFLDGMTRARRKILAGGVKCFASNNRERKVFQFGGYVADHMFYHHGDMSLNTSIIKAAQYYPGSSHLYPVFIGIGSFGSRHLGGKDAGSPRYISVQLASEFIKTMFPAEDSWLLPYVFEDGQRAEPEYYVPVLPLAIMEYGANPSEGWKYTTWARQLEDILALVRAYVDKDNPKHELLHYAIKHKITILPLRPSNYNFKGHLKRFGQYYYSYGTYDISEQRNIITITELPLRVPTVAYIESIKKSSNRMTFIEEIIDYSSSETIEILVKLKPNSLNRIVEEFKETEEQDSIENFLRLRNCLHSHLNFVKPKGGIIEFNSYYEILYAWLPYRRELYQKRLMREHAVLKLRIIMETAIVRYINESAELNLSHYEDEKEASRILSEHGFPPLNHTLIISPEFASIEELNQKALQGCYTYILSLQARELLIAAKTRRVEKIKKMQARLDKVEQLLQESPFPGASVWLEEIDAVEKAIIKGRNTQWKFH

Function

Type II topoisomerase. Processively relaxes supercoiled DNA. Displays DNA-supercoiling activity only when associated with the viral histone-like protein.
Type II topoisomerase (PubMed:27060564, PubMed:25463606). Processively relaxes supercoiled DNA (PubMed:27060564). Displays DNA-supercoiling activity only when associated with the viral histone-like protein (PubMed:28381576).

Catalytic Activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Similarity

Belongs to the type II topoisomerase family.

Keywords

ATP-binding Complete proteome DNA-binding Host cytoplasm Isomerase Late protein Magnesium Metal-binding Nucleotide-binding Topoisomerase Reference proteome

Feature

chain DNA topoisomerase 2

Uniprot

D4I5U9 P34203 A9JM23 A0A0A7HFG9 A0A0A1E3Q0 Q00942 + More
A9JL87 P0C9C1 P0C9C0 P0C9C2 A0A2Z5DGM7 A0A0C5B080 E0WM62 A0A2X0THW2 A0A0C5AZJ7

EC Number

5.99.1.3

Pubmed

1335084 8021596 18198370 25463606 25406173 26618713 + More
1316688 11831707 27060564 28381576 25645905 21470447

EMBL

FN557520 CBH29212.1 Z14245 X71982 AY261361 AM712240 + More
CAN10461.1 KM261522 AIZ03629.1 KM262844 KM262845 KM102979 KP055815 KX354450 AIY22303.1 AIY22461.1 AJZ77044.1 AKO62793.1 AOO54473.1 M88699 U18466 AM712239 CAN10212.1 AY261366 AY261363 AY261360 MH025919 AXB49858.1 KM111295 MH025916 MH025917 MH025918 MH025920 AJL34290.1 AXB49341.1 AXB49515.1 AXB49687.1 AXB50031.1 FR682468 CBW46778.1 MG939583 MG939588 LS478113 AXZ95885.1 AXZ96168.1 SPS73535.1 KM111294 AJL34125.1

Proteomes

UP000101090 UP000000860 UP000108903 UP000110401 UP000117635 UP000142390 + More
UP000241813 UP000266411 UP000000624 UP000130745 UP000000858 UP000000859 UP000000861 UP000101566 UP000141072 UP000105860

PRIDE

P0C9C2

Pfam

PF16898   TOPRIM_C
PF00521   DNA_topoisoIV
PF02518   HATPase_c

Interpro

IPR036890   HATPase_C_sf
IPR013760   Topo_IIA-like_dom_sf
IPR013757   Topo_IIA_A_a_sf
IPR014721   Ribosomal_S5_D2-typ_fold_subgr
IPR013758   Topo_IIA_A/C_ab
IPR001241   Topo_IIA
IPR003594   HATPase_C
IPR002205   Topo_IIA_A/C
IPR013759   Topo_IIA_B_C
IPR031660   TOPRIM_C
IPR018522   TopoIIA_CS
IPR020568   Ribosomal_S5_D2-typ_fold

SUPFAM

SSF56719   SSF56719
SSF55874   SSF55874
SSF54211   SSF54211

Gene 3D

1.10.268.10 3.30.230.10 3.30.565.10 3.40.50.670 3.90.199.10

ProteinModelPortal

D4I5U9 P34203 A9JM23 A0A0A7HFG9 A0A0A1E3Q0 Q00942 + More
A9JL87 P0C9C1 P0C9C0 P0C9C2 A0A2Z5DGM7 A0A0C5B080 E0WM62 A0A2X0THW2 A0A0C5AZJ7

PANTHER

PTHR10169

PDB

4GFH E-value=1.48562e-78, Score=750

Ontologies

KEGG

vg:22220341

GO

GO:0005524  F:ATP binding
GO:0006265  P:DNA topological change
GO:0003677  F:DNA binding
GO:0003918  F:DNA topoisomerase type II (ATP-hydrolyzing) activity
GO:0030430  C:host cell cytoplasm
GO:0046872  F:metal ion binding

Subcellular Location

From MSLVP

Capsid

From Uniprot

Host cytoplasm Localizes to the cytoplasmic viral factories. With evidence from 3 publications.

Topology

Length:

1192

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.31152

Exp number, first 60 AAs:

0.1446

Total prob of N-in:

0.00926

outside

1 - 1192

Population Genetic Test Statistics

Pi

0.0333857

Theta

0.0375462

Tajima's D

-0.583893

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62793.1
China_AnhuiXCGQ_2018	Protein	AYW34083.1
China_ASFV-SY18_2018	Protein	AKO62793.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95885.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10212.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10212.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10212.1
Poland_Pol17_03029_C201_2016-2017	Protein	CAN10212.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96168.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10212.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10212.1
Uganda_R8_2015	Protein	AXB49341.1
Uganda_R7_2015	Protein	AXB49515.1
Uganda_R25_2015	Protein	AXB49687.1
Uganda_N10_2015	Protein	AXB49858.1
Uganda_R35_2015	Protein	AXB50031.1
Estonia_2014	Protein	SPS73535.1
Italy_26544_OG10_2010	Protein	AJZ77044.1
Italy_47_Ss_2008_2008	Protein	AOO54473.1
Portugal_OURT_88.3_1988	Protein	CAN10461.1
Spain_E75_1975	Protein	CBH29212.1
Russia_Georgia_2007	Protein	CBW46778.1
Kenya_Tk1_2005	Protein	AJL34125.1
Kenya_Bus_2006	Protein	AJL34290.1
Russia_Odintsovo_2014	Protein	CAN10212.1
Portugal_L60_1960	Protein	AIY22303.1
Portugal_NHV_1968	Protein	AIY22461.1
Russia_Kashino_2013	Protein	CAN10212.1
Benin_1997	Protein Protein	CAN10212.1 CAN10212.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10212.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10212.1
Malawi_Lil_1983	Protein	CAN10212.1
Kenya_1950	Protein	CAN10212.1
Namibia_Warthog_1980	Protein	CAN10212.1
South_Africa_Warmbaths_1987	Protein	CAN10212.1
Malawi_Tengani_1962	Protein	CAN10212.1
China_HLJ_2018	Protein	QBH90600.1
China_LN_2018	Protein	QBH90785.1
China_wbBS01_2018	Protein	QDL88143.1
Ukraine_Kyiv_2014	Protein Protein Protein Protein	QED21716.1 QED21717.1 QED21718.1 QED21719.1
Belgium_Etalle_wb_2018	Protein	QED90555.1
Lithuania_LT14_2014	Protein	QEY87918.1
South_Africa_1985	Protein	QGM12932.1
South_Africa_2_2008	Protein	QGM12858.1
Zambia_LIV_1983

Sequence