ASFVdb The African Swine Fever Virus Database
Strain
Ukraine_Kyiv_2014 (Region: Ukraine;  Strain: ASFV/Kyiv/2016/131;  Date: 2014)
Gene
EP1242L
Description
Annotated in NCBI,  ASFV_Kyiv_2016_131_00078
GenBank Accession
Full name
DNA-directed RNA polymerase subunit beta       + More
DNA-directed RNA polymerase subunit 2      
DNA-directed RNA polymerase subunit B      
 

Sequence

CDS
ATGGCAAAAGACATTTTAGCCGGAACGCTTACACTTGAAGATCTCGTAGCACAGGGCATCTGTGAGTTCATTACCCCCGAGGAGGCGGAAAACTGTCTTGTTGCCTTCTCCATCATCGAGCTACGCAAACATAAACATGATGTAACGCGTCGTTTCACACACGTGGACGTGCCCCAAGCTATTTTGGGCCTTGCCGCCCTAGTGTCCCCCTACGCAAACTGTACCCAACCTGCGCGTGTTACCTATGAAACAAACCAAGGCCGGCAAACGGGGGGCTGGTATTGCTTTAGCTGGCCATATCGTGTCGACATGAACCGCTTCTTCCAGTTTTACAATGAAATGCCGCTTGTAAAGACCATAGCACATAACTATGTCATACCCAACGGGCTTAATACCATTGTGGCTTATATGATCTACGGCGGCTACAATCAGGAGGACTCTGTGATCGTGTCGCAGTCCTTTATTGACAGGGGCGGCTTTGCCGGCACCTTCTACCGAGAAGAAAAAGTAGAATTAGAAAGCGATATAGAGTCGTTTGGGAAGCCCGACCCTTTAATCACCAAAAATCTTAAGCCTGGTGCTAATTACGAAAAGCTTGTAGATGGATTCGTGCCCGTAGGAACGGTGGTCAAAAAGGGCGACATCATTATAGGAAAAGTGGCCAAAATACGGGGCGAAAAAGACGAACTCAACAAATACATCGACCGGTCTGTCATGTACGGCTTCGATGAGCCGGCCGTCGTGGATGCTGTGATGCGTCCCCATGGGCCTAATGACGAGATCTTTGGCCTCATGCGCCTGCGCTATGAAAGAAATCTCAATATAGGCGACAAAATGAGTTCTCGCAGCGGGAACAAGGGCATTGCGGCGCTGGCCCTGCCCACTTCAGACATGCCCTTCACAGAGGACGGATTGCAGCCCGACCTCATTGTAAACCCCCACTCACATCCCTCCCGAATGACCAACGGTCAAATGATAGAAACCACGGTTGGTCTAGCAAATGCCCTACAGGGGGTGGTAACAGACGGCACCGCATTCCTGCCTATTAATGTGCAGCTTCTTTCAGAAAGACTGGCGCAAGAAGGTCTACGCTTCAATGGATGCCAAAAAATGTTCAACGGCCAGACCGGCGAGTACTTTGATGCGGCCATCTTCATAGGACCGACCTATCACCAGCGCCTGCAAAAATTTGTGCTGGATGACCGATACGCCGTTGCAAGCTATGGGCCCACGGATGCCCTCACAGGCCAGCCGTTGGACGGCAAACGATCGCACGGAGGTCTTCGTCTGGGAGAAATGGAGCATTGGGTGCTTACGGCGCAGGGCGCCATGCAAACCATCATAGAAAAATCGCACGACGACTCGGATGGCTGCATCTCCTACATCTGTAGAAACTGTGGCGAGCCTGCCATCTATAACGCGAGTCATCCCATCTACAAGTGCATGAACTGTGATGTCCAGGCCGATATCGGCATGGTAGATAGCCGTCGCTCTTCCATCGTATTTCAACACGAAATGCGAGCGGCCAATGTTAATATTACATCCGTGCTTTCACCAAGAGTTTTTCAACCAGCTTAA
Protein
MAKDILAGTLTLEDLVAQGICEFITPEEAENCLVAFSIIELRKHKHDVTRRFTHVDVPQAILGLAALVSPYANCTQPARVTYETNQGRQTGGWYCFSWPYRVDMNRFFQFYNEMPLVKTIAHNYVIPNGLNTIVAYMIYGGYNQEDSVIVSQSFIDRGGFAGTFYREEKVELESDIESFGKPDPLITKNLKPGANYEKLVDGFVPVGTVVKKGDIIIGKVAKIRGEKDELNKYIDRSVMYGFDEPAVVDAVMRPHGPNDEIFGLMRLRYERNLNIGDKMSSRSGNKGIAALALPTSDMPFTEDGLQPDLIVNPHSHPSRMTNGQMIETTVGLANALQGVVTDGTAFLPINVQLLSERLAQEGLRFNGCQKMFNGQTGEYFDAAIFIGPTYHQRLQKFVLDDRYAVASYGPTDALTGQPLDGKRSHGGLRLGEMEHWVLTAQGAMQTIIEKSHDDSDGCISYICRNCGEPAIYNASHPIYKCMNCDVQADIGMVDSRRSSIVFQHEMRAANVNITSVLSPRVFQPA

Summary

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit 2 is the second largest component of RNA polymerase. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. DNA-dependent RNA polymerase is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
Catalytic Activity
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Zn(2+)
Subunit
Component of the RNA polymerase that consists of at least 6 subunits.
The S.acidocaldarius RNAP is composed of 13 subunits.
Miscellaneous
The binding of ribonucleoside triphosphate to the RNA polymerase transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol subunits (By similarity).
Similarity
Belongs to the RNA polymerase beta chain family.
Keywords
Complete proteome   DNA-directed RNA polymerase   Host cytoplasm   Metal-binding   Nucleotidyltransferase   Transcription   Transferase   Zinc   Zinc-finger   Reference proteome  
Feature
chain  DNA-directed RNA polymerase subunit 2
EC Number
2.7.7.6
EMBL
MN194591    QED21614.1    AM712239    CAN10153.1    FN557520    CBH29154.1    + More
AM712240    CAN10401.1    KM262844    KM262845    KP055815    AIY22244.1    AIY22403.1    AKO62735.1    KM102979    KX354450    AJZ77042.1    AOO54384.1    AY261366    AY261363    Z21490    U18466    FR682468    CBW46719.1    MG939583    MG939587    MG939588    LS478113    AXZ95829.1    AXZ96017.1    AXZ96112.1    SPS73476.1    AY261361    MH025916    MH025917    MH025918    MH025919    MH025920    AXB49282.1    AXB49456.1    AXB49628.1    AXB49799.1    AXB49972.1    KM111295    AJL34232.1    AY261360    KM111294    AJL34067.1    ANIY01003006    ETP37619.1    KI669605    ETN04142.1    KU702948    KU702952    AMN82980.1    AMN84950.1    KJ614390    KU702950    AIB51752.1    AMN83967.1    ANJA01002649    ETO68411.1    LT839607    SME64703.1    GL376588    KU702949    KU702951    KU556803    AMN83870.1    AMN84468.1    AMP44390.1    CZDJ02000003    SMH63388.1    KX552040    ARA72182.1    NJBG01000001    TKJ16953.1    QZIM01000013    RJQ21011.1    RFJL01000033    RME78118.1    CP002098    ADM28051.1    PCXZ01000033    PIN86044.1    NJDL01000002    OYT32825.1    NZDG01000051    MAG78570.1    NEXM01000016    PSN99376.1    NEXD01000008    PSN86315.1    NEXA01000130    PSN91788.1    MNWW01000002    OIO66765.1    NEXB01000001    PSN89641.1    NEXC01000001    PSN84569.1    PFNP01000218    PIZ53873.1    CP001140    ACL11701.1    CP003321    AFL67335.1    NZCX01000024    MAG40013.1    AE017199    AAR39011.1    RFJB01000153    RMF05022.1    NYYQ01000051    MAE42961.1    CP002817    AGE70642.1    CP002818    AGE72915.1    CP013694    CP013695    ALU29008.1    ALU31735.1    X14818    CP000077    BA000023    BAB65244.1    QMXI01000022    RLG99816.1    QMZW01000031    RLJ05875.1    BEWT01000012    GAY25882.1    QFWU01000013    PWV37534.1    BARV01011732    GAI11508.1    PEZQ01000008    PIN99822.1    QMXY01000024    RLI21975.1    JWKP01000004    KHO46961.1    SOKQ01000014    TES83859.1    NZER01000235    MAH47818.1    NJEC01000016    OYT57167.1    QMYG01000048    RLI30699.1    NEXF01000265    PSO07373.1    KY476720    AQS32809.1    NJED01000003    OYT60966.1    QMRG01000064    RLE63892.1    CP001901    ADC70329.1    QMRP01000007    RLE82738.1    QMSB01000011    RLF00351.1    AP006487    BAM79323.1    KY476704    AQS31468.1    KY476713    AQS32379.1    QMXR01000044    RLI13260.1    QMYV01000391    RLI58600.1    AMCR01022013    EJY64666.1    CP001939    ADG91667.1   
Pfam
PF04560   RNA_pol_Rpb2_7
PF00562   RNA_pol_Rpb2_6
PF04566   RNA_pol_Rpb2_4
PF04565   RNA_pol_Rpb2_3
PF04563   RNA_pol_Rpb2_1
PF04561   RNA_pol_Rpb2_2
PF04567   RNA_pol_Rpb2_5
PF14528   LAGLIDADG_3
Interpro
IPR014724   RNA_pol_RPB2_OB-fold
IPR007120   DNA-dir_RNAP_su2_dom
IPR015712   DNA-dir_RNA_pol_su2
IPR037033   DNA-dir_RNAP_su2_hyb_sf
IPR007121   RNA_pol_bsu_CS
IPR007641   RNA_pol_Rpb2_7
IPR007644   RNA_pol_bsu_protrusion
IPR007642   RNA_pol_Rpb2_2
IPR007646   RNA_pol_Rpb2_4
IPR037034   RNA_pol_Rpb2_2_sf
IPR007645   RNA_pol_Rpb2_3
IPR007647   RNA_pol_Rpb2_5
IPR004042   Intein_endonuc
IPR006142   INTEIN
IPR027434   Homing_endonucl
IPR036844   Hint_dom_sf
IPR004860   LAGLIDADG_2
IPR019969   RNAP_B
SUPFAM
SSF55608   SSF55608
SSF51294   SSF51294
PDB
6EXV     E-value=4.96956e-125,     Score=1151

Ontologies

KEGG

Subcellular Location

From MSLVP
Capsid
From Uniprot
Host cytoplasm  

Topology

Length:
525
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.328050000000001
Exp number, first 60 AAs:
0.01257
Total prob of N-in:
0.01417
outside
1  -  525
 
 

Population Genetic Test Statistics

Pi
0.0325293
Theta
0.0455105
Tajima's D
-0.98812
CLR
0.056983
Interpretation
No evidence of Selection

Multiple alignment of Orthologues

 
 

Gene Tree

 
 

Orthologous in Strains

Strain Availability Status Gene
Spain_BA71_1971 Protein
Protein
QED21615.1
AKO62735.1
China_AnhuiXCGQ_2018 Protein
Protein
QED21615.1
AYW34025.1
China_ASFV-SY18_2018 Protein
AKO62735.1
Poland_Pol16_20186_o7_2016-2017 Protein
Protein
QED21615.1
AXZ95829.1
Poland_Pol16_20538_o9_2016-2017 Protein
Protein
CAN10153.1
QED21615.1
Poland_Pol16_20540_o10_2016-2017 Protein
Protein
CAN10153.1
QED21615.1
Poland_Pol16_29413_o23_2016-2017 Protein
Protein
CAN10153.1
QED21615.1
Poland_Pol17_03029_C201_2016-2017 Protein
Protein
QED21615.1
AXZ96017.1
Poland_Pol17_04461_C210_2016-2017 Protein
Protein
QED21615.1
AXZ96112.1
Poland_Pol17_05838_C220_2016-2017 Protein
Protein
CAN10153.1
QED21615.1
Poland_ASFV_POL_Podlaskie_2015 Protein
Protein
CAN10153.1
QED21615.1
Uganda_R8_2015 Protein
Protein
QED21615.1
AXB49282.1
Uganda_R7_2015 Protein
Protein
QED21615.1
AXB49456.1
Uganda_R25_2015 Protein
Protein
QED21615.1
AXB49628.1
Uganda_N10_2015 Protein
Protein
QED21615.1
AXB49799.1
Uganda_R35_2015 Protein
Protein
QED21615.1
AXB49972.1
Estonia_2014 Protein
Protein
QED21615.1
SPS73476.1
Italy_26544_OG10_2010 Protein
Protein
QED21615.1
AJZ77042.1
Italy_47_Ss_2008_2008 Protein
Protein
QED21615.1
AOO54384.1
Portugal_OURT_88.3_1988 Protein
Protein
QED21615.1
CAN10401.1
Spain_E75_1975 Protein
Protein
QED21615.1
CBH29154.1
Russia_Georgia_2007 Protein
Protein
QED21615.1
CBW46719.1
Kenya_Tk1_2005 Protein
Protein
QED21615.1
AJL34067.1
Kenya_Bus_2006 Protein
Protein
QED21615.1
AJL34232.1
Russia_Odintsovo_2014 Protein
Protein
CAN10153.1
QED21615.1
Portugal_L60_1960 Protein
Protein
QED21615.1
AIY22244.1
Portugal_NHV_1968 Protein
Protein
QED21615.1
AIY22403.1
Russia_Kashino_2013 Protein
Protein
CAN10153.1
QED21615.1
Benin_1997 Protein
Protein
Protein
CAN10153.1
QED21615.1
CAN10153.1
South_Africa_KNP_Pretorisuskop_1996 Protein
Protein
CAN10153.1
QED21615.1
South_Africa_MGR_Mkuzi_1979 Protein
Protein
CAN10153.1
QED21615.1
Malawi_Lil_1983 Genetic Remain
Protein
CAN10153.1
QED21615.1
Kenya_1950 Protein
Protein
CAN10153.1
QED21615.1
Namibia_Warthog_1980 Protein
Protein
CAN10153.1
QED21615.1
South_Africa_Warmbaths_1987 Protein
Protein
CAN10153.1
QED21615.1
Malawi_Tengani_1962 Protein
Protein
CAN10153.1
QED21615.1
China_HLJ_2018 Protein
Protein
QED21615.1
QBH90541.1
China_LN_2018 Protein
Protein
QED21615.1
QBH90726.1
China_wbBS01_2018 Protein
Protein
QED21615.1
QDL88084.1
Ukraine_Kyiv_2014 Protein
QED21614.1
Belgium_Etalle_wb_2018 Protein
Protein
QED21615.1
QED90496.1
Lithuania_LT14_2014 Protein
Protein
QED21615.1
QEY87859.1
South_Africa_1985 Protein
Genetic Remain
CAN10153.1
QED21615.1
South_Africa_2_2008 Protein
Genetic Remain
CAN10153.1
QED21615.1
Zambia_LIV_1983 Protein
Protein
CAN10153.1
QED21615.1
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