Strain

Ukraine_Kyiv_2014 (Region: Ukraine;  Strain: ASFV/Kyiv/2016/131;  Date: 2014)

Gene

EP1242L

Description

Annotated in NCBI,  ASFV_Kyiv_2016_131_00078

Location

MN194591.1(-):68189-69766

GenBank Accession

QED21614.1

Full name

DNA-directed RNA polymerase subunit beta       + More
DNA-directed RNA polymerase subunit 2      
DNA-directed RNA polymerase subunit B      

CDS

ATGGCAAAAGACATTTTAGCCGGAACGCTTACACTTGAAGATCTCGTAGCACAGGGCATCTGTGAGTTCATTACCCCCGAGGAGGCGGAAAACTGTCTTGTTGCCTTCTCCATCATCGAGCTACGCAAACATAAACATGATGTAACGCGTCGTTTCACACACGTGGACGTGCCCCAAGCTATTTTGGGCCTTGCCGCCCTAGTGTCCCCCTACGCAAACTGTACCCAACCTGCGCGTGTTACCTATGAAACAAACCAAGGCCGGCAAACGGGGGGCTGGTATTGCTTTAGCTGGCCATATCGTGTCGACATGAACCGCTTCTTCCAGTTTTACAATGAAATGCCGCTTGTAAAGACCATAGCACATAACTATGTCATACCCAACGGGCTTAATACCATTGTGGCTTATATGATCTACGGCGGCTACAATCAGGAGGACTCTGTGATCGTGTCGCAGTCCTTTATTGACAGGGGCGGCTTTGCCGGCACCTTCTACCGAGAAGAAAAAGTAGAATTAGAAAGCGATATAGAGTCGTTTGGGAAGCCCGACCCTTTAATCACCAAAAATCTTAAGCCTGGTGCTAATTACGAAAAGCTTGTAGATGGATTCGTGCCCGTAGGAACGGTGGTCAAAAAGGGCGACATCATTATAGGAAAAGTGGCCAAAATACGGGGCGAAAAAGACGAACTCAACAAATACATCGACCGGTCTGTCATGTACGGCTTCGATGAGCCGGCCGTCGTGGATGCTGTGATGCGTCCCCATGGGCCTAATGACGAGATCTTTGGCCTCATGCGCCTGCGCTATGAAAGAAATCTCAATATAGGCGACAAAATGAGTTCTCGCAGCGGGAACAAGGGCATTGCGGCGCTGGCCCTGCCCACTTCAGACATGCCCTTCACAGAGGACGGATTGCAGCCCGACCTCATTGTAAACCCCCACTCACATCCCTCCCGAATGACCAACGGTCAAATGATAGAAACCACGGTTGGTCTAGCAAATGCCCTACAGGGGGTGGTAACAGACGGCACCGCATTCCTGCCTATTAATGTGCAGCTTCTTTCAGAAAGACTGGCGCAAGAAGGTCTACGCTTCAATGGATGCCAAAAAATGTTCAACGGCCAGACCGGCGAGTACTTTGATGCGGCCATCTTCATAGGACCGACCTATCACCAGCGCCTGCAAAAATTTGTGCTGGATGACCGATACGCCGTTGCAAGCTATGGGCCCACGGATGCCCTCACAGGCCAGCCGTTGGACGGCAAACGATCGCACGGAGGTCTTCGTCTGGGAGAAATGGAGCATTGGGTGCTTACGGCGCAGGGCGCCATGCAAACCATCATAGAAAAATCGCACGACGACTCGGATGGCTGCATCTCCTACATCTGTAGAAACTGTGGCGAGCCTGCCATCTATAACGCGAGTCATCCCATCTACAAGTGCATGAACTGTGATGTCCAGGCCGATATCGGCATGGTAGATAGCCGTCGCTCTTCCATCGTATTTCAACACGAAATGCGAGCGGCCAATGTTAATATTACATCCGTGCTTTCACCAAGAGTTTTTCAACCAGCTTAA

Protein

MAKDILAGTLTLEDLVAQGICEFITPEEAENCLVAFSIIELRKHKHDVTRRFTHVDVPQAILGLAALVSPYANCTQPARVTYETNQGRQTGGWYCFSWPYRVDMNRFFQFYNEMPLVKTIAHNYVIPNGLNTIVAYMIYGGYNQEDSVIVSQSFIDRGGFAGTFYREEKVELESDIESFGKPDPLITKNLKPGANYEKLVDGFVPVGTVVKKGDIIIGKVAKIRGEKDELNKYIDRSVMYGFDEPAVVDAVMRPHGPNDEIFGLMRLRYERNLNIGDKMSSRSGNKGIAALALPTSDMPFTEDGLQPDLIVNPHSHPSRMTNGQMIETTVGLANALQGVVTDGTAFLPINVQLLSERLAQEGLRFNGCQKMFNGQTGEYFDAAIFIGPTYHQRLQKFVLDDRYAVASYGPTDALTGQPLDGKRSHGGLRLGEMEHWVLTAQGAMQTIIEKSHDDSDGCISYICRNCGEPAIYNASHPIYKCMNCDVQADIGMVDSRRSSIVFQHEMRAANVNITSVLSPRVFQPA

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit 2 is the second largest component of RNA polymerase. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. DNA-dependent RNA polymerase is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)

Cofactor

Zn(2+)

Subunit

Component of the RNA polymerase that consists of at least 6 subunits.
The S.acidocaldarius RNAP is composed of 13 subunits.

Miscellaneous

The binding of ribonucleoside triphosphate to the RNA polymerase transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol subunits (By similarity).

Similarity

Belongs to the RNA polymerase beta chain family.

Keywords

Complete proteome   DNA-directed RNA polymerase   Host cytoplasm   Metal-binding   Nucleotidyltransferase   Transcription   Transferase   Zinc   Zinc-finger   Reference proteome  

Feature

chain  DNA-directed RNA polymerase subunit 2

Uniprot

A0A5B8XA69   A9JL02   D4I5P1   A9JLR5   A0A0A1E066   A0A1C6ZXW3   + More
P0C8K5   P0C8K4   P42487   E0WMJ1   A0A2X0RU95   P0C8K3   A0A2Z5DG98   A0A0C5AZ99   P0C8K2   A0A0C5AYW2   W2YUK5   W2PT56   A0A141ZIB2   A0A0H3TLG3   A0A080ZP50   A0A1X7BZ16   K3X6E1   A0A142C0P0   A0A1X7QFP1   A0A1V0CPA2   A0A532T2Q0   A0A3A4UP55   A0A3M1HA34   E0SPC4   A0A2G9N4N3   A0A256XX99   A0A2D6RMB3   A0A2R6BL91   A0A2R6AIW4   A0A2R6AZS9   A0A1J4Y7R0   A0A2R6ATD6   A0A2R6AE06   A0A2H9PTK8   B8D6H0   I3XTR1   A0A2D6NH67   Q74MN2   A0A3M1JF22   A0A2D5XE53   M1IPB9   M1JAY7   A0A0U3H235   P11513   Q976A4   A0A497KAX2   A0A497TC38   A0A2R4ZS74   A0A2V2XUS7   X1KXT4   A0A2G9PA94   A0A497M431   A0A0B3AEH2   A0A523QEP3   A0A2D6XA77   A0A256ZXK8   A0A497MXJ8   A0A2R6C917   A0A1S6HBB0   A0A257A816   A0A497FI24   D3S665   A0A497FXV1   A0A497G366   M1VFL8   A0A1S6H7S8   A0A1S6HA27   A0A497LDW0   A0A497Q2M7   J9EEU9   D5U3G7  

EC Number

2.7.7.6

Pubmed

18198370    25406173    26618713    8506138    11831707    21470447    + More
25645905    26903952    25878099    20626842    27801826    28644444    21304693    27112493    19114480    23012283    14566062    22418622    2501756    15995215    11572479    24624126    28835260    15071595    17623057    23382650    21304709   

EMBL

MN194591    QED21614.1    AM712239    CAN10153.1    FN557520    CBH29154.1    + More
AM712240    CAN10401.1    KM262844    KM262845    KP055815    AIY22244.1    AIY22403.1    AKO62735.1    KM102979    KX354450    AJZ77042.1    AOO54384.1    AY261366    AY261363    Z21490    U18466    FR682468    CBW46719.1    MG939583    MG939587    MG939588    LS478113    AXZ95829.1    AXZ96017.1    AXZ96112.1    SPS73476.1    AY261361    MH025916    MH025917    MH025918    MH025919    MH025920    AXB49282.1    AXB49456.1    AXB49628.1    AXB49799.1    AXB49972.1    KM111295    AJL34232.1    AY261360    KM111294    AJL34067.1    ANIY01003006    ETP37619.1    KI669605    ETN04142.1    KU702948    KU702952    AMN82980.1    AMN84950.1    KJ614390    KU702950    AIB51752.1    AMN83967.1    ANJA01002649    ETO68411.1    LT839607    SME64703.1    GL376588    KU702949    KU702951    KU556803    AMN83870.1    AMN84468.1    AMP44390.1    CZDJ02000003    SMH63388.1    KX552040    ARA72182.1    NJBG01000001    TKJ16953.1    QZIM01000013    RJQ21011.1    RFJL01000033    RME78118.1    CP002098    ADM28051.1    PCXZ01000033    PIN86044.1    NJDL01000002    OYT32825.1    NZDG01000051    MAG78570.1    NEXM01000016    PSN99376.1    NEXD01000008    PSN86315.1    NEXA01000130    PSN91788.1    MNWW01000002    OIO66765.1    NEXB01000001    PSN89641.1    NEXC01000001    PSN84569.1    PFNP01000218    PIZ53873.1    CP001140    ACL11701.1    CP003321    AFL67335.1    NZCX01000024    MAG40013.1    AE017199    AAR39011.1    RFJB01000153    RMF05022.1    NYYQ01000051    MAE42961.1    CP002817    AGE70642.1    CP002818    AGE72915.1    CP013694    CP013695    ALU29008.1    ALU31735.1    X14818    CP000077    BA000023    BAB65244.1    QMXI01000022    RLG99816.1    QMZW01000031    RLJ05875.1    BEWT01000012    GAY25882.1    QFWU01000013    PWV37534.1    BARV01011732    GAI11508.1    PEZQ01000008    PIN99822.1    QMXY01000024    RLI21975.1    JWKP01000004    KHO46961.1    SOKQ01000014    TES83859.1    NZER01000235    MAH47818.1    NJEC01000016    OYT57167.1    QMYG01000048    RLI30699.1    NEXF01000265    PSO07373.1    KY476720    AQS32809.1    NJED01000003    OYT60966.1    QMRG01000064    RLE63892.1    CP001901    ADC70329.1    QMRP01000007    RLE82738.1    QMSB01000011    RLF00351.1    AP006487    BAM79323.1    KY476704    AQS31468.1    KY476713    AQS32379.1    QMXR01000044    RLI13260.1    QMYV01000391    RLI58600.1    AMCR01022013    EJY64666.1    CP001939    ADG91667.1   

Proteomes

UP000321214    UP000130745    UP000101090    UP000108903    UP000110401    UP000142390    + More
UP000241813    UP000117635    UP000266411    UP000000858    UP000000859    UP000000624    UP000141072    UP000000860    UP000101566    UP000000861    UP000105860    UP000018948    UP000018817    UP000244713    UP000244774    UP000244303    UP000244833    UP000028582    UP000019132    UP000244556    UP000244783    UP000244814    UP000204182    UP000317367    UP000266049    UP000275188    UP000001304    UP000229410    UP000216128    UP000226806    UP000240381    UP000240569    UP000240838    UP000182568    UP000241473    UP000240880    UP000228584    UP000006903    UP000006175    UP000221720    UP000000578    UP000269239    UP000226537    UP000011281    UP000011280    UP000060043    UP000065473    UP000001018    UP000001015    UP000267990    UP000270304    UP000245022    UP000246840    UP000228724    UP000272007    UP000319603    UP000226719    UP000216854    UP000280664    UP000242015    UP000237430    UP000275342    UP000002189    UP000271663    UP000282419    UP000007014    UP000266938    UP000269983    UP000006077    UP000002376   

PRIDE

P0C8K3    P11513   

Pfam

PF04560   RNA_pol_Rpb2_7
PF00562   RNA_pol_Rpb2_6
PF04566   RNA_pol_Rpb2_4
PF04565   RNA_pol_Rpb2_3
PF04563   RNA_pol_Rpb2_1
PF04561   RNA_pol_Rpb2_2
PF04567   RNA_pol_Rpb2_5
PF14528   LAGLIDADG_3

Interpro

IPR014724   RNA_pol_RPB2_OB-fold
IPR007120   DNA-dir_RNAP_su2_dom
IPR015712   DNA-dir_RNA_pol_su2
IPR037033   DNA-dir_RNAP_su2_hyb_sf
IPR007121   RNA_pol_bsu_CS
IPR007641   RNA_pol_Rpb2_7
IPR007644   RNA_pol_bsu_protrusion
IPR007642   RNA_pol_Rpb2_2
IPR007646   RNA_pol_Rpb2_4
IPR037034   RNA_pol_Rpb2_2_sf
IPR007645   RNA_pol_Rpb2_3
IPR007647   RNA_pol_Rpb2_5
IPR004042   Intein_endonuc
IPR006142   INTEIN
IPR027434   Homing_endonucl
IPR036844   Hint_dom_sf
IPR004860   LAGLIDADG_2
IPR019969   RNAP_B

SUPFAM

SSF55608   SSF55608
SSF51294   SSF51294

Gene 3D

2.40.270.10     2.40.50.150     3.90.1110.10     3.10.28.10    

CDD

cd00653  RNA_pol_B_RPB2

ProteinModelPortal

A0A5B8XA69   A9JL02   D4I5P1   A9JLR5   A0A0A1E066   A0A1C6ZXW3   + More
P0C8K5   P0C8K4   P42487   E0WMJ1   A0A2X0RU95   P0C8K3   A0A2Z5DG98   A0A0C5AZ99   P0C8K2   A0A0C5AYW2   W2YUK5   W2PT56   A0A141ZIB2   A0A0H3TLG3   A0A080ZP50   A0A1X7BZ16   K3X6E1   A0A142C0P0   A0A1X7QFP1   A0A1V0CPA2   A0A532T2Q0   A0A3A4UP55   A0A3M1HA34   E0SPC4   A0A2G9N4N3   A0A256XX99   A0A2D6RMB3   A0A2R6BL91   A0A2R6AIW4   A0A2R6AZS9   A0A1J4Y7R0   A0A2R6ATD6   A0A2R6AE06   A0A2H9PTK8   B8D6H0   I3XTR1   A0A2D6NH67   Q74MN2   A0A3M1JF22   A0A2D5XE53   M1IPB9   M1JAY7   A0A0U3H235   P11513   Q976A4   A0A497KAX2   A0A497TC38   A0A2R4ZS74   A0A2V2XUS7   X1KXT4   A0A2G9PA94   A0A497M431   A0A0B3AEH2   A0A523QEP3   A0A2D6XA77   A0A256ZXK8   A0A497MXJ8   A0A2R6C917   A0A1S6HBB0   A0A257A816   A0A497FI24   D3S665   A0A497FXV1   A0A497G366   M1VFL8   A0A1S6H7S8   A0A1S6HA27   A0A497LDW0   A0A497Q2M7   J9EEU9   D5U3G7  

PANTHER

PTHR20856  

PDB

6EXV     E-value=4.96956e-125,     Score=1151