Strain

Lithuania_LT14_2014 (Region: Lithuania;  Strain: ASFV/LT14/1490;  Date: 2014)

Gene

EP1242L

Description

Annotated in NCBI,  NA

Location

MK628478.1(-):66536-70264

GenBank Accession

QEY87859.1

Full name

DNA-directed RNA polymerase subunit beta       + More
DNA-directed RNA polymerase subunit 2      

CDS

ATGGAACCCTTGCGGCCGCAAATAACCTACGGCCCCATTGAAACCGTGGATAATGAGGAGCTTACAGAGGCTGATATGCTGAGCTTTATCTCCGCTGCAGTAAACTCTACAGGATTGATAGGGTATAACATCAAAAGCTTCGACGATCTTATGGACAATGGAATTCCGCAAATTGTAAAACAAATGTTTAACGTGGATATTACTTATAAAGACCAACGCGACCATACTGAAATAGATAAGCTTCGGGAATCAGTTCAGATCCAATTTAACTTTACAGATGTGAACATTGAACGCCCGCAACATCGAAACTATTCACAGGGAAACAAGATAAATTTACTACCCAATAAGGCACGCCTGTGTGGCCTTTCCTATTCGGGCCCGGTTAATCTAGCCGCCGAGGTTATTTTAACGGCGCACTACAGCAATGGTCGGCAGGAGGTAAAACGAGCAAGTATTCCTCCTTTTCAGGTATCTACATTTCCCATCATGCGGGGCAGCAACCGCTGCCACACTCATCACCTATCAAAAACCGCAAAAAAAGAAATAGGAGAAGACCCTAATGAACCCGGCGGCTACTTTATAGCCCGAGGCGGGGAATGGGTCGTAGACCTCTTAGAAAACATCCGCTTTAACACGCTTCACATCCATTACCATACCATGCAACAAGGGAATAATGAAATCATTCGCGGCGAGTTTATCTCCCAACCGGGAGGTGCCTTCGAAAATAGCTCTCAAATTATTATACGCTACATGACCACCGGCGCCATTACCATTGAAATCAATAGCACCAAGTTCTCAAAACTTCGCATACCTTGGTATCTCATCTTTCGCATGTTTGGAATGACAGGCGATGACAGCATCATTGAGCAAGTGGTATTTGATCTAGAATCCAACTCCCTTGTGAATACCTTCATGATAGAAATTCTGGAAAAATCCATTCATGTTTTAGACCCCATATTTCAACCGGTCCAACACGAGCTGAACCGAGAAAAAATTATCCAGTTTTTATCCGAGAAGGTTTCCAAATTTGTCTCAAACCCATCTGCTTACAAAAGCGATGAAAATGCGGTACAATACTTAAATGAAAGACAACTTACTATTTTAGACAAAATATTGCTGCCACACATGGGCCAAACCGCAGATACACGTGTACGAAAGCTGCGCTTTTTAGGCCTGCTTATTCATAAAATACTTCTTGTCATTATGAATGTGTTTCCACCCACGGACCGTGATAGCTACCGCACCAAGCGGGTGCACGGCTCAGGGGTCTCACTCGCCAAGGCCTTTAAGGCTATTTTTAATACTTCCGTGATAGCACCCATCATCAATGGGTTCAAAGAACTTCTTAAACAAACAGCCTTTGAGGAGCTTACTCAGCGAAACATTATCGAGGCATTTTCTGCCGCTTTATCAAAAAATACCGCCTCCGATCTTAATCGCAGTATGGAACAGTCTATCATTTCAGGAAATAAAACCATCATGGTGCGACAGCGCCCCATTGTAAACAGAGTATCTACTCAAAGTTTGGAAAGAAAAAATTTATTAAATACCATTTCAGCATTGCGTACGGTAAATACTCATAACACCACCAACGCATCCAAACAAACAGAAAGGGCTGACATGATGCGTCGCGTGCACGCATCCTATCCTGGATACATCTGCGTAGCCCAGTCAGCCGATACTGGTGAAAAAGTAGGCATGTCTAAACAGTTAGCCATTACTGCCAATGTCTGCACCGCCGGAGAAGTACTTTCGTTAAAACAACGCCTTCTATCAGATCCGGCCATTCAACAGTTAGCCGATGTTTCCAACAAGGACATTGTACGAAAGGGCCTAGCGCGCGTATTTATCAACGGAGAATGGATCGGCTGCTGCACCAATGCCTTTGAGCTTGCTCAGCGGTATAGAATGCTTCGCCGTGAGGGCAAGGTTGTGCATCCCCATACCACCATCTACTGGGATAGCATGGTAGACGAAGTAGAATTTTGGTTGGACGTCGGTAGGCTGACCCGCCCCCTACTCATTGTAGATAATAATATTGAAAAATACAATCAAGCCTGCTACAAGGCCGCAGAGGCCAGAAAAAAAGGCGATAAAGACTGGGAAAAACATAAAATCCCTTTCATTCAAAATACGCGATTTACGCCTCAAATGGCAAAAGACATTTTAGCCGGAACGCTTACACTTGAAGATCTCGTAGCACAGGGCATCTGTGAGTTCATTACCCCCGAGGAGGCGGAAAACTGTCTTGTTGCCTTCTCCATCATCGAGCTACGCAAACATAAACATGATGTAACGCGTCGTTTCACACACGTGGACGTGCCCCAAGCTATTTTGGGCCTTGCCGCCCTAGTGTCCCCCTACGCAAACTGTACCCAACCTGCGCGTGTTACCTATGAAACAAACCAAGGCCGGCAAACGGGGGGCTGGTATTGCTTTAGCTGGCCATATCGTGTCGACATGAACCGCTTCTTCCAGTTTTACAATGAAATGCCGCTTGTAAAGACCATAGCACATAACTATGTCATACCCAACGGGCTTAATACCATTGTGGCTTATATGATCTACGGCGGCTACAATCAGGAGGACTCTGTGATCGTGTCGCAGTCCTTTATTGACAGGGGCGGCTTTGCCGGCACCTTCTACCGAGAAGAAAAAGTAGAATTAGAAAGCGATATAGAGTCGTTTGGGAAGCCCGACCCTTTAATCACCAAAAATCTTAAGCCTGGTGCTAATTACGAAAAGCTTGTAGATGGATTCGTGCCCGTAGGAACGGTGGTCAAAAAGGGCGACATCATTATAGGAAAAGTGGCCAAAATACGGGGCGAAAAAGACGAACTCAACAAATACATCGACCGGTCTGTCATGTACGGCTTCGATGAGCCGGCCGTCGTGGATGCTGTGATGCGTCCCCATGGGCCTAATGACGAGATCTTTGGCCTCATGCGCCTGCGCTATGAAAGAAATCTCAATATAGGCGACAAAATGAGTTCTCGCAGCGGGAACAAGGGCATTGCGGCGCTGGCCCTGCCCACTTCAGACATGCCCTTCACAGAGGACGGATTGCAGCCCGACCTCATTGTAAACCCCCACTCACATCCCTCCCGAATGACCAACGGTCAAATGATAGAAACCACGGTTGGTCTAGCAAATGCCCTACAGGGGGTGGTAACAGACGGCACCGCATTCCTGCCTATTAATGTGCAGCTTCTTTCAGAAAGACTGGCGCAAGAAGGTCTACGCTTCAATGGATGCCAAAAAATGTTCAACGGCCAGACCGGCGAGTACTTTGATGCGGCCATCTTCATAGGACCGACCTATCACCAGCGCCTGCAAAAATTTGTGCTGGATGACCGATACGCCGTTGCAAGCTATGGGCCCACGGATGCCCTCACAGGCCAGCCGTTGGACGGCAAACGATCGCACGGAGGTCTTCGTCTGGGAGAAATGGAGCATTGGGTGCTTACGGCGCAGGGCGCCATGCAAACCATCATAGAAAAATCGCACGACGACTCGGATGGCTGCATCTCCTACATCTGTAGAAACTGTGGCGAGCCTGCCATCTATAACGCGAGTCATCCCATCTACAAGTGCATGAACTGTGATGTCCAGGCCGATATCGGCATGGTAGATAGCCGTCGCTCTTCCATCGTATTTCAACACGAAATGCGAGCGGCCAATGTTAATATTACATCCGTGCTTTCACCAAGAGTTTTTCAACCAGCTTAA

Protein

MEPLRPQITYGPIETVDNEELTEADMLSFISAAVNSTGLIGYNIKSFDDLMDNGIPQIVKQMFNVDITYKDQRDHTEIDKLRESVQIQFNFTDVNIERPQHRNYSQGNKINLLPNKARLCGLSYSGPVNLAAEVILTAHYSNGRQEVKRASIPPFQVSTFPIMRGSNRCHTHHLSKTAKKEIGEDPNEPGGYFIARGGEWVVDLLENIRFNTLHIHYHTMQQGNNEIIRGEFISQPGGAFENSSQIIIRYMTTGAITIEINSTKFSKLRIPWYLIFRMFGMTGDDSIIEQVVFDLESNSLVNTFMIEILEKSIHVLDPIFQPVQHELNREKIIQFLSEKVSKFVSNPSAYKSDENAVQYLNERQLTILDKILLPHMGQTADTRVRKLRFLGLLIHKILLVIMNVFPPTDRDSYRTKRVHGSGVSLAKAFKAIFNTSVIAPIINGFKELLKQTAFEELTQRNIIEAFSAALSKNTASDLNRSMEQSIISGNKTIMVRQRPIVNRVSTQSLERKNLLNTISALRTVNTHNTTNASKQTERADMMRRVHASYPGYICVAQSADTGEKVGMSKQLAITANVCTAGEVLSLKQRLLSDPAIQQLADVSNKDIVRKGLARVFINGEWIGCCTNAFELAQRYRMLRREGKVVHPHTTIYWDSMVDEVEFWLDVGRLTRPLLIVDNNIEKYNQACYKAAEARKKGDKDWEKHKIPFIQNTRFTPQMAKDILAGTLTLEDLVAQGICEFITPEEAENCLVAFSIIELRKHKHDVTRRFTHVDVPQAILGLAALVSPYANCTQPARVTYETNQGRQTGGWYCFSWPYRVDMNRFFQFYNEMPLVKTIAHNYVIPNGLNTIVAYMIYGGYNQEDSVIVSQSFIDRGGFAGTFYREEKVELESDIESFGKPDPLITKNLKPGANYEKLVDGFVPVGTVVKKGDIIIGKVAKIRGEKDELNKYIDRSVMYGFDEPAVVDAVMRPHGPNDEIFGLMRLRYERNLNIGDKMSSRSGNKGIAALALPTSDMPFTEDGLQPDLIVNPHSHPSRMTNGQMIETTVGLANALQGVVTDGTAFLPINVQLLSERLAQEGLRFNGCQKMFNGQTGEYFDAAIFIGPTYHQRLQKFVLDDRYAVASYGPTDALTGQPLDGKRSHGGLRLGEMEHWVLTAQGAMQTIIEKSHDDSDGCISYICRNCGEPAIYNASHPIYKCMNCDVQADIGMVDSRRSSIVFQHEMRAANVNITSVLSPRVFQPA

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit 2 is the second largest component of RNA polymerase. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. DNA-dependent RNA polymerase is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)

Subunit

Component of the RNA polymerase that consists of at least 6 subunits.

Miscellaneous

The binding of ribonucleoside triphosphate to the RNA polymerase transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol subunits (By similarity).

Similarity

Belongs to the RNA polymerase beta chain family.

Keywords

Complete proteome   DNA-directed RNA polymerase   Host cytoplasm   Metal-binding   Nucleotidyltransferase   Transcription   Transferase   Zinc   Zinc-finger   Reference proteome  

Feature

chain  DNA-directed RNA polymerase subunit 2

Uniprot

A0A0C5AYW2   P0C8K5   P0C8K4   P0C8K2   E0WMJ1   A0A2X0RU95   + More
A0A0C5AZ99   A0A2Z5DG98   A0A1C6ZXW3   A9JL02   D4I5P1   A9JLR5   A0A0A1E066   P42487   P0C8K3  

EC Number

2.7.7.6

Pubmed

25645905    21470447    18198370    25406173    26618713    8506138    + More
11831707   

EMBL

KM111294    AJL34067.1    AY261366    AY261363    AY261360    FR682468    + More
CBW46719.1    MG939583    MG939587    MG939588    LS478113    AXZ95829.1    AXZ96017.1    AXZ96112.1    SPS73476.1    KM111295    AJL34232.1    MH025916    MH025917    MH025918    MH025919    MH025920    AXB49282.1    AXB49456.1    AXB49628.1    AXB49799.1    AXB49972.1    KM102979    KX354450    AJZ77042.1    AOO54384.1    AM712239    CAN10153.1    FN557520    CBH29154.1    AM712240    CAN10401.1    KM262844    KM262845    KP055815    AIY22244.1    AIY22403.1    AKO62735.1    Z21490    U18466    AY261361   

Proteomes

UP000105860    UP000000858    UP000000859    UP000000861    UP000141072    UP000101566    + More
UP000117635    UP000266411    UP000130745    UP000101090    UP000108903    UP000110401    UP000142390    UP000241813    UP000000624    UP000000860   

PRIDE

P0C8K3   

Pfam

PF04560   RNA_pol_Rpb2_7
PF04563   RNA_pol_Rpb2_1
PF04565   RNA_pol_Rpb2_3
PF04566   RNA_pol_Rpb2_4
PF04561   RNA_pol_Rpb2_2
PF00562   RNA_pol_Rpb2_6

Interpro

IPR007641   RNA_pol_Rpb2_7
IPR007644   RNA_pol_bsu_protrusion
IPR007121   RNA_pol_bsu_CS
IPR007642   RNA_pol_Rpb2_2
IPR037034   RNA_pol_Rpb2_2_sf
IPR014724   RNA_pol_RPB2_OB-fold
IPR007646   RNA_pol_Rpb2_4
IPR007645   RNA_pol_Rpb2_3
IPR015712   DNA-dir_RNA_pol_su2
IPR037033   DNA-dir_RNAP_su2_hyb_sf
IPR007120   DNA-dir_RNAP_su2_dom

Gene 3D

2.40.270.10     2.40.50.150     3.90.1110.10    

ProteinModelPortal

A0A0C5AYW2   P0C8K5   P0C8K4   P0C8K2   E0WMJ1   A0A2X0RU95   + More
A0A0C5AZ99   A0A2Z5DG98   A0A1C6ZXW3   A9JL02   D4I5P1   A9JLR5   A0A0A1E066   P42487   P0C8K3  

PANTHER

PTHR20856  

PDB

6EXV     E-value=4.96956e-125,     Score=1151