Strain

Lithuania_LT14_2014 (Region: Lithuania; Strain: ASFV/LT14/1490; Date: 2014)

Gene

F334L

Description

Annotated in NCBI, NA

Location

MK628478.1(-):55993-56997

GenBank Accession

QEY87850.1

Full name

Ribonucleoside-diphosphate reductase small chain + More
Ribonucleoside-diphosphate reductase subunit beta

Alternative Name

Ribonucleotide reductase small subunit

CDS

ATGTTAATATTTATTTCAAATATGGAGGAATTACTAATTGAAAATTCCCAACGTTTCACTATTTTTCCTATTCAGCACCCCGAATGTTGGAACTGGTATAAAAAATTAGAAAGTTTGACGTGGACGGCGCAGGAGGTGGACATGTGTAAGGACATTGATGATTGGGAAGCCATGCCAAAGCCACAGCGCGAATTTTATAAACAAATTTTAGCCTTCTTTGTTGTTGCCGACGAAATTGTTATCGAAAACCTTTTAACCAATTTTATGCGCGAAATTAAAGTAAAGGAAGTGTTATACTTTTATACAATGCAGGCTGCCCAAGAGTGCGTTCATAGCGAAGCCTACTCAATACAGGTAAAAACGCTGATCCCGGATGAGAAGGAACAACAGCGCATCTTTAGCGGCATTGAAAAACACCCTATCATAAAAAAAATGGCGCAGTGGGTGCGGCAGTGGATGGACCCCGACAGGAACACTCTAGGTGAGCGGCTGGTGGGCTTCGCCGCCGTAGAAGGCATTTTATTTCAAAACCACTTTGTGGCGATCCAGTTTCTTAAGGAACAGAATATTATGCCGGGGCTTGTAAGCTATAATGAGTTTATCTCACGTGATGAAGGGATGCACTGTAGTTTTGCGTGTTTTCTTATCTCAAATTACGTGTATAACATTCCCGAGGAAAAGATCATTCACAAAATTTTGAAGGAGGCTGTGGAGTTGGTGGATGAGTTTATTAACTATGCCTTTGACAAGGCAAGAGGAAGAGTTCCCGGTTTTTCAAAAGAAATGCTATTTCAATATATACGCTACTTTACAGATAATTTGTGCTTCATGATGCAGTGCAAAAGCATTTATAAGGTGGGAAACCCTTTTCCTCAGATGACTAAATTCTTTCTTAATGAAGTAGAAAAAACCAACTTTTTCGAGTTAAGACCTACGCAGTATCAGAACTGTGTTAAGGATGATGCGTTTGCCTTCAAACTCTTTTTAAACGACGATGATTTTTAA

Protein

MLIFISNMEELLIENSQRFTIFPIQHPECWNWYKKLESLTWTAQEVDMCKDIDDWEAMPKPQREFYKQILAFFVVADEIVIENLLTNFMREIKVKEVLYFYTMQAAQECVHSEAYSIQVKTLIPDEKEQQRIFSGIEKHPIIKKMAQWVRQWMDPDRNTLGERLVGFAAVEGILFQNHFVAIQFLKEQNIMPGLVSYNEFISRDEGMHCSFACFLISNYVYNIPEEKIIHKILKEAVELVDEFINYAFDKARGRVPGFSKEMLFQYIRYFTDNLCFMMQCKSIYKVGNPFPQMTKFFLNEVEKTNFFELRPTQYQNCVKDDAFAFKLFLNDDDF

Function

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic Activity

[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate

Cofactor

Fe cation

Subunit

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).

Similarity

Belongs to the ribonucleoside diphosphate reductase small chain family.

Keywords

Complete proteome DNA replication Iron Metal-binding Oxidoreductase Reference proteome

Feature

chain Ribonucleoside-diphosphate reductase small chain

Uniprot

D4I5N2 A9JLN9 E0WMI2 A0A0A1E0M7 P42492 A0A5B8XAR1 + More
P0C8I2 A0A1C6ZY12 A9JKY6 P26713 A0A0C5BCL6 P0C8I0 A0A0C5BC64 P0C8I1 M1JSJ7 A0A1A9Y0F4 A0A1B0BB24 D3TQH9 A0A1B0FQN1 A0A1B0A7I9 A0A223FML1 Q91BG2 A0A223FN71 I4DQD3 A0A1A9UY57 A0A2G2J464 A0A2G1VVU8 A0A194QA43 A0A4P8NQ48 A0A212FIQ5 H9JVE7 A0A498HJF3 A0A540LUW0 A0A519M869 A0A2E3F0Z3 A0A519T8N5 A0A0K8TMM2 A0A140HQZ1 S4PCX5 A0A540KW72 A0A519RWZ3 A0A182PIU1 A0A4Q3JPE0 A0A182FFL1 A0A328B6Y1 G3EI98 A0A4C1T8Y6 A0A498J176 A0A2H1W8N5 A0A358Y5L0 A0A3D9LHD1 A0A1D8QNI3 A0A519MHP2 A0A2S1XBD3 A0A140HRH7 A0A1B9E4J1 A0A4V1F1H6 V9TIJ5 A0A1D8P8V2 Q9YMK7 A0A3S2LZT2 A0A140HQG9 A0A0D3QW76 A0A4P8NQE6 A0A513WWF5 A0A1S5YDT4 A0A0A0YZ80 A0A0L7LTX7 A0A495VGY0 A0A2T5YT83 A0A076HQM0 A0A142EIC8 A0A2W1BFZ9 A0A3R7XHS5 A0A1A9WBA5 R1BJX5 A0A484DRK8 W8EVA9 A0A3S0HM69 A0A1I2TZW7 A0A1C9KC71 T1DRH5 A0A353F9F7 A0A2M4ASK8 A0A182X294 A0A519MDW7 A0A519T6Z6 A0A182WMC5 W5J0Z2 A0A4Y9QHL9 A0A519MHY4 A0A4R8I3N4 A0A4V3ASC0 A0A182I6F5 A0A2A4JLF6 A0A182K769 A0A349NQK5 A0A2M4CHV7 A0A558BV97 A0A2T2VQT0

EC Number

1.17.4.1

Pubmed

18198370 21470447 25406173 26618713 11831707 1871976 + More
25645905 23219924 20353571 28762208 11531416 22651552 29099490 26354079 22118469 19121390 24081323 24773378 30498029 26369729 23622113 21813608 31044173 30148503 26728722 24370838 9887315 25908142 28392286 25593255 26227816 27174266 28756777 28713657 23760476 27613417 20920257 23761445

EMBL

FN557520 CBH29145.1 AM712240 CAN10392.1 FR682468 CBW46710.1 + More
KM262844 KM262845 KP055815 KX354450 MG939583 MG939587 MG939588 LS478113 AIY22235.1 AIY22394.1 AKO62726.1 AOO54374.1 AXZ95820.1 AXZ96008.1 AXZ96103.1 SPS73467.1 U18466 MN194591 QED21600.1 AY261366 KM102979 AJZ77097.1 AM712239 CAN10143.1 M64728 AY261361 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34223.1 AXB49273.1 AXB49447.1 AXB49619.1 AXB49790.1 AXB49963.1 AY261360 KM111294 AJL34058.1 AY261363 JX454574 AGE89912.1 JXJN01011284 EZ423681 ADD19957.1 CCAG010009454 MF001304 AST09231.1 AF325155 AAL01745.1 MF001305 AST09433.1 AK404052 BAM20123.1 NVVZ01000139 PHR93058.1 NQXA01000001 PHQ30906.1 KQ459465 KPJ00301.1 MK411291 QCQ67370.1 AGBW02008342 OWR53617.1 BABH01036043 BABH01036044 BABH01036045 BABH01036046 AB703263 BAL72672.1 RDQH01000343 RXH69625.1 VIEB01000462 TQD90072.1 SEBX01000061 RZJ57757.1 PAPI01000004 MBA80142.1 SECF01000191 RZK31374.1 GDAI01002180 JAI15423.1 KT626571 AMO27788.1 GAIX01003906 JAA88654.1 VIEB01000906 TQD78473.1 SEBY01000789 RZK15833.1 SEBF01000123 RYE90506.1 QHKM01000012 RAK62657.1 HQ849551 AEN03609.1 BGZK01004799 GBP10973.1 RDQH01000335 RXH89250.1 ODYU01007043 SOQ49459.1 DORI01000167 HBW86445.1 QREG01000001 REE05914.1 KX618634 AOW42794.1 SECA01000130 RZJ61038.1 MF311096 AWJ76733.1 KT626572 KU862282 AMO27974.1 AMO65605.1 LVEP01000019 OCB76849.1 MK411293 QCQ67689.1 KF695050 AHC69623.1 CP017478 AOW21016.1 AF081810 AAC70306.1 RSAL01000095 RVE47802.1 KT626570 AMO27616.1 KP027546 AJR20393.1 MK411292 QCQ67530.1 MK264918 QDH05963.1 KY249580 AQQ80135.1 KM386655 AIX47958.1 JTDY01000137 KOB78661.1 RBXM01000001 RKT48631.1 QBKI01000001 PTX22513.1 CP006587 AII52115.1 CP012836 AMQ54883.1 KZ150213 PZC72157.1 NHJR02000004 RPG62716.1 KB868468 EOD10158.1 SHOA01000120 TDH65069.1 CP007145 AHJ95667.1 RXOF01000009 RTQ48510.1 FOOT01000003 SFG70422.1 KU749311 AOP31730.1 GAMD01000630 JAB00961.1 DNQR01000097 HBF19142.1 GGFK01010436 MBW43757.1 SECA01000305 RZJ59466.1 SECD01001503 RZK30680.1 ADMH02002207 ETN57812.1 SPSB01000006 TFV92161.1 SECA01000119 RZJ61152.1 SOEL01000014 TDX70294.1 SMUW01000023 TDK49917.1 APCN01003635 NWSH01001095 PCG72649.1 DMXF01000252 HAT65069.1 GGFL01000736 MBW64914.1 VMRJ01000003 TVT40440.1 PXYR01000010 PSR08030.1

Proteomes

UP000101090 UP000108903 UP000141072 UP000110401 UP000142390 UP000241813 + More
UP000266411 UP000000624 UP000321214 UP000000858 UP000117635 UP000130745 UP000000860 UP000101566 UP000000861 UP000105860 UP000000859 UP000232896 UP000092443 UP000092460 UP000092444 UP000092445 UP000217350 UP000202667 UP000217248 UP000078200 UP000229433 UP000053268 UP000007151 UP000005204 UP000290289 UP000315295 UP000230854 UP000075885 UP000292505 UP000069272 UP000248553 UP000164653 UP000299102 UP000256779 UP000093510 UP000176050 UP000203997 UP000283053 UP000037510 UP000267061 UP000244225 UP000028590 UP000073816 UP000091820 UP000013827 UP000294530 UP000019423 UP000282184 UP000198724 UP000203649 UP000076407 UP000075920 UP000000673 UP000297647 UP000295004 UP000295438 UP000075840 UP000218220 UP000075881 UP000317624 UP000242248

PRIDE

H9JVE7

Pfam

PF00268 Ribonuc_red_sm

Interpro

IPR009078   Ferritin-like_SF
IPR000358   RNR_small_fam
IPR012348   RNR-like
IPR033909   RNR_small
IPR030475   RNR_small_AS

SUPFAM

SSF47240 SSF47240

Gene 3D

1.10.620.20

CDD

cd01049 RNRR2

ProteinModelPortal

D4I5N2 A9JLN9 E0WMI2 A0A0A1E0M7 P42492 A0A5B8XAR1 + More
P0C8I2 A0A1C6ZY12 A9JKY6 P26713 A0A0C5BCL6 P0C8I0 A0A0C5BC64 P0C8I1 M1JSJ7 A0A1A9Y0F4 A0A1B0BB24 D3TQH9 A0A1B0FQN1 A0A1B0A7I9 A0A223FML1 Q91BG2 A0A223FN71 I4DQD3 A0A1A9UY57 A0A2G2J464 A0A2G1VVU8 A0A194QA43 A0A4P8NQ48 A0A212FIQ5 H9JVE7 A0A498HJF3 A0A540LUW0 A0A519M869 A0A2E3F0Z3 A0A519T8N5 A0A0K8TMM2 A0A140HQZ1 S4PCX5 A0A540KW72 A0A519RWZ3 A0A182PIU1 A0A4Q3JPE0 A0A182FFL1 A0A328B6Y1 G3EI98 A0A4C1T8Y6 A0A498J176 A0A2H1W8N5 A0A358Y5L0 A0A3D9LHD1 A0A1D8QNI3 A0A519MHP2 A0A2S1XBD3 A0A140HRH7 A0A1B9E4J1 A0A4V1F1H6 V9TIJ5 A0A1D8P8V2 Q9YMK7 A0A3S2LZT2 A0A140HQG9 A0A0D3QW76 A0A4P8NQE6 A0A513WWF5 A0A1S5YDT4 A0A0A0YZ80 A0A0L7LTX7 A0A495VGY0 A0A2T5YT83 A0A076HQM0 A0A142EIC8 A0A2W1BFZ9 A0A3R7XHS5 A0A1A9WBA5 R1BJX5 A0A484DRK8 W8EVA9 A0A3S0HM69 A0A1I2TZW7 A0A1C9KC71 T1DRH5 A0A353F9F7 A0A2M4ASK8 A0A182X294 A0A519MDW7 A0A519T6Z6 A0A182WMC5 W5J0Z2 A0A4Y9QHL9 A0A519MHY4 A0A4R8I3N4 A0A4V3ASC0 A0A182I6F5 A0A2A4JLF6 A0A182K769 A0A349NQK5 A0A2M4CHV7 A0A558BV97 A0A2T2VQT0

PANTHER

PTHR23409

PDB

1XSM E-value=1.16382e-65, Score=634

Ontologies

KEGG

vg:22220426 vg:922197 vg:11107163 vg:1488565 vg:29063987

GO

GO:0009263  P:deoxyribonucleotide biosynthetic process
GO:0016491  F:oxidoreductase activity
GO:0046872  F:metal ion binding
GO:0004748  F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
GO:0006260  P:DNA replication
GO:0016021  C:integral component of membrane

Subcellular Location

From MSLVP

Cytoplasm

Topology

Length:

334

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.13489

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00845

outside

1 - 334

Population Genetic Test Statistics

Pi

0.0263481

Theta

0.0409595

Tajima's D

-0.8193632

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62726.1
China_AnhuiXCGQ_2018	Protein	AYW34016.1
China_ASFV-SY18_2018	Protein	AKO62726.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95820.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10392.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10392.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10392.1
Poland_Pol17_03029_C201_2016-2017	Protein	AXZ96008.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96103.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10392.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10392.1
Uganda_R8_2015	Protein	AXB49273.1
Uganda_R7_2015	Protein	AXB49447.1
Uganda_R25_2015	Protein	AXB49619.1
Uganda_N10_2015	Protein	AXB49790.1
Uganda_R35_2015	Protein	AXB49963.1
Estonia_2014	Protein	SPS73467.1
Italy_26544_OG10_2010	Protein	AJZ77097.1
Italy_47_Ss_2008_2008	Protein	AOO54374.1
Portugal_OURT_88.3_1988	Protein Protein	CAN10392.1 CAN10392.1
Spain_E75_1975	Protein	CBH29145.1
Russia_Georgia_2007	Protein	CBW46710.1
Kenya_Tk1_2005	Protein	AJL34058.1
Kenya_Bus_2006	Protein	AJL34223.1
Russia_Odintsovo_2014	Protein	CAN10392.1
Portugal_L60_1960	Protein	AIY22235.1
Portugal_NHV_1968	Protein	AIY22394.1
Russia_Kashino_2013	Protein	CAN10392.1
Benin_1997	Protein	CAN10143.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10392.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10392.1
Malawi_Lil_1983	Protein	CAN10392.1
Kenya_1950	Protein	CAN10392.1
Namibia_Warthog_1980	Protein	CAN10392.1
South_Africa_Warmbaths_1987	Protein	CAN10392.1
Malawi_Tengani_1962	Protein	CAN10392.1
China_HLJ_2018	Protein	QBH90532.1
China_LN_2018	Protein	QBH90717.1
China_wbBS01_2018	Protein	QDL88075.1
Ukraine_Kyiv_2014	Protein	QED21600.1
Belgium_Etalle_wb_2018	Protein	QED90487.1
Lithuania_LT14_2014	Protein	QEY87850.1
South_Africa_1985	Protein	CAN10392.1
South_Africa_2_2008	Protein	CAN10392.1
Zambia_LIV_1983	Protein	CAN10392.1

Sequence