Strain

South_Africa_1985 (Region: South Africa; Strain: SPEC_57; Date: 1985)

Gene

pNP868R

Description

Annotated in NCBI, NP868R

Location

MN394630.1(+):132390-134996

GenBank Accession

QGM12928.1

Full name

Probable mRNA-capping enzyme

CDS

ATGGCTTCTTTACATATTTTACTGGCACGATATCAGAGGTGCTTTATTGTCCAGTCTCTTACAAATACTACTATTGTACTTGCAATACGTTTTCAACAGATATATTTTTTATTATTCAAAACCGTATATGTGGCACTTGTGGCACAAGAGATCCCTACCACCATCTCCCACAGCATCCGCTGCATCAAAAAAGTTCACCATGCAAACCACTGCCGGGAAAAAATTTTGCCGTCGGAGAATCTTTACTTCAAAAAACAGCCTCTCATGTTTTTTATGTTTTCAGAGCCTGCATCTCTGGGCTGTATGGTCTCGCTGGCCATCGAGCAGCCCATTCGTATATTTATCTTGGACTCCTCCGTTCTCGTTCGGCTCAAAAATCGTACGACCTTTCGGGTATCTGTACTTTGGAAAATACAGCTTACCATTGTACAGCAGCTGTTGGGAAGCGAGGTCTCTGCAAAACTTACCGCCTTCAAAACGCTTCTGTTTGTCACCCCAGAGCAACAAACGACAAAAAATATGTTGTCGTTATTATACCCAGATGTCGAATATCTTTACGAAATACAAATACAGTATACAGGAAAGCCCGAATCCCTATCGGCGGCAGATATTATACAAATTACAAACACGGTGTTGTCGCTTATTTCTCCGAACCATTTATTGCTATCAGCCTACCACCAGGCCATTGTATTCATTGCCTCCCATATACTGTCTTCAGAAATCCTTCTTGCACGTATTACGAGCGGGAAGTGGGGGCTTATACGCCTCCTTCCCCAGGTGCAATCCATGTCCAAAGCAGATTACATGCAATTTTATCCGCCTGTTGGTTACTATATATCGGACAAAGCAGATGGAATTACAGGCATCGCCGTCATTCAGGACACGCAAATTTATGTGGTTGCAGACCAGCTTTACAGCCTACGTACCACCGGCATTGTACCCCTTATACCAACCATTTTGGACGGTGTATTTATGCCTGCAAAAAAAGAATTTTATGGGTTTGTTGTCATCATGTATGTGGGCAATCTATTGTCGCAACAGGGGTTTGCAACAAGAATTGTGTCTTTATGCAAGGGCATTACAGCCTTACAAGCATTTATCATACAAGCAGAAATGCAGCCCTTTATTTCGCTATCAAGTGCAGATCCCAACGTGCTGCTCAAAAACTTTGCAAGTATTTTTACGAAAAAAACTCGCCCGTATTCTATTGTTGGCATCATTTTACTACAACCTGGCAATTCTTATCTATATACAAACACTTTTACGTGGAAGCCCACCTGGGATATCACATTACACTTTTTGGTGCGAAAATGTCCAGAGAGTTTATACGTACCAGAGTACGCGCCCAAAAAAGGGTTTTCCCTGCATCTACTATTTGTACGCATCTCCGGAGAGCTTTTTACGAAATTACCGCTATATTGGTGTCCAGGATACACGAAACTATTCCCCGTTACACAGCGCAACCAAAACTACTTTCCAGTACAGTTTCAGCCATCGGATTTTCCATTGGCATTTCTTTATTACCACCCAGATACCTCATCATTTTCTGTTATACATGGAAAGGTCCTTGCAATGCGTTGTCTTACGAGAGAAATCAATCATATCAGCTGGGAAATTGTACAAATCCGGGAGGATACGCAGCAGGATCTTACAACCGGCGGGTATTTTGGTATTGTTTTCAAAACAGCCGAACTCACATGGCTTATCTATATGGATCCCTTTTCCTTTGTGGAGCTGGCAAAGGGCCCTTCTGGAATGTACTTCGCCGGTGCCAAAACCGGCATATACCGCGCTCAAACAGCACTTATTTCCTTTATTATGCAAGAAATCATCCAAAAAATATGTCACCAATCCTGGGTTATCGATCTTGGAATACGAAAGGGGCAGGACCTACGACGCTACCTGGACGCAGGGGTATGGCATCTTGTTGGGATCGATATGGATCAAACCGCGCTCGCAGAGCTTGTTTATCGAAAGTTTTCGCATGCTACAACCCGACAGCACAAGCACGCTACCAACATTTATGTGTTGCATCAAGACCTTGCAGAGCCTGCGAAGGAAATCAGCGAAAAGGTACACCAAATTTACGGGTTTCCCAAGGAGGGAGCTTCTTCCATTGTTACCAACCTGTTTATTCACTATCTTATGCAAAACACGCAGCAGGTGGAAAACCTGGCCGTTTTGTGCCATATGCTTCTTCAGCCGGGGGGAATGGTGTGGTTTACCACCATGCTGGGAGAACGGGTCTTACAATTACTTCATGCAAATACAATACAGCTCAATGCAGTATGGGAGGCACGTGCAAACGAGGTGGTCAAATTTGCTATTATACGTCTCTTTACAGAGGATATATTACAGGAAACTGGGCAAGAAATTGGAGTCCTGTTACCCTTCAGCAATGGCGACTTCTACAATGTATATCTTGTGTACACAGCATTTTTATTTACAATATTTATACATCACGGGTTTTCCCTGGTCCAAAAGCAGTCCTTTATGGACTGGATTCCAGAATTTCAAAACTTTACTACAAGTTTGTATACAATTCTTACAGAAGCCGATACAACTTGGACAAGCCTTTTTGGGTTTATTTGTCTGCGCAAAAATTAA

Protein

MASLHILLARYQRCFIVQSLTNTTIVLAIRFQQIYFLLFKTVYVALVAQEIPTTISHSIRCIKKVHHANHCREKILPSENLYFKKQPLMFFMFSEPASLGCMVSLAIEQPIRIFILDSSVLVRLKNRTTFRVSVLWKIQLTIVQQLLGSEVSAKLTAFKTLLFVTPEQQTTKNMLSLLYPDVEYLYEIQIQYTGKPESLSAADIIQITNTVLSLISPNHLLLSAYHQAIVFIASHILSSEILLARITSGKWGLIRLLPQVQSMSKADYMQFYPPVGYYISDKADGITGIAVIQDTQIYVVADQLYSLRTTGIVPLIPTILDGVFMPAKKEFYGFVVIMYVGNLLSQQGFATRIVSLCKGITALQAFIIQAEMQPFISLSSADPNVLLKNFASIFTKKTRPYSIVGIILLQPGNSYLYTNTFTWKPTWDITLHFLVRKCPESLYVPEYAPKKGFSLHLLFVRISGELFTKLPLYWCPGYTKLFPVTQRNQNYFPVQFQPSDFPLAFLYYHPDTSSFSVIHGKVLAMRCLTREINHISWEIVQIREDTQQDLTTGGYFGIVFKTAELTWLIYMDPFSFVELAKGPSGMYFAGAKTGIYRAQTALISFIMQEIIQKICHQSWVIDLGIRKGQDLRRYLDAGVWHLVGIDMDQTALAELVYRKFSHATTRQHKHATNIYVLHQDLAEPAKEISEKVHQIYGFPKEGASSIVTNLFIHYLMQNTQQVENLAVLCHMLLQPGGMVWFTTMLGERVLQLLHANTIQLNAVWEARANEVVKFAIIRLFTEDILQETGQEIGVLLPFSNGDFYNVYLVYTAFLFTIFIHHGFSLVQKQSFMDWIPEFQNFTTSLYTILTEADTTWTSLFGFICLRKN

Function

Catalyzes the second reaction in the mRNA cap formation pathway.

Catalytic Activity

a 5'-triphospho-(purine-ribonucleotide)-[mRNA] + H2O = a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + H(+) + phosphate
a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + GTP + H(+) = a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + diphosphate
a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-homocysteine

Similarity

In the N-terminal section; belongs to the dsDNA virus mRNA guanylyltransferase family.
In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.

Keywords

Complete proteome GTP-binding Hydrolase Methyltransferase mRNA capping mRNA processing Nucleotide-binding Nucleotidyltransferase RNA-binding S-adenosyl-L-methionine Transferase Reference proteome

Feature

chain Probable mRNA-capping enzyme

Uniprot

D4I5T8 A0A0A1DYG7 A9JM02 A0A0N7CR23 A0A0A1DXR2 A9JL69 + More
A0A1C6ZXW9 P0C993 P32094 P0C994 E0WM51 A0A2X0TKL6 Q65216 P0C995 A0A0C5AWD7 A0A2Z5DGW7 A0A0C5AWT8

Pubmed

25406173 18198370 26618713 8382399 11831707 21470447 + More
8021596 25645905

EMBL

FN557520 CBH29201.1 KM262845 AIY22450.1 AM712240 CAN10450.1 + More
KP055815 AKO62782.1 KM262844 AIY22292.1 AM712239 CAN10200.1 KM102979 KX354450 AJZ77049.1 AOO54458.1 AY261363 L07263 U18466 AY261366 FR682468 CBW46767.1 MG939583 MG939588 LS478113 AXZ95875.1 AXZ96158.1 SPS73524.1 X71982 AY261361 AY261360 KM111294 AJL34114.1 MH025916 MH025917 MH025918 MH025919 MH025920 AXB49329.1 AXB49503.1 AXB49675.1 AXB49846.1 AXB50019.1 KM111295 AJL34279.1

Proteomes

UP000101090 UP000110401 UP000108903 UP000241813 UP000142390 UP000130745 + More
UP000117635 UP000266411 UP000000859 UP000000624 UP000000858 UP000141072 UP000000860 UP000000861 UP000105860 UP000101566

PRIDE

P0C995

Pfam

PF01331 mRNA_cap_enzyme
PF03291 Pox_MCEL

Interpro

IPR001339   mRNA_cap_enzyme
IPR004971   mRNA_G-N7_MeTrfase_dom
IPR033469   CYTH-like_dom_sf
IPR029063   SAM-dependent_MTases

SUPFAM

SSF55154 SSF55154
SSF53335 SSF53335

ProteinModelPortal

D4I5T8 A0A0A1DYG7 A9JM02 A0A0N7CR23 A0A0A1DXR2 A9JL69 + More
A0A1C6ZXW9 P0C993 P32094 P0C994 E0WM51 A0A2X0TKL6 Q65216 P0C995 A0A0C5AWD7 A0A2Z5DGW7 A0A0C5AWT8

PDB

2HV9 E-value=6.37495e-06, Score=123

Ontologies

KEGG

vg:22220330

GO

GO:0006370  P:7-methylguanosine mRNA capping
GO:0004484  F:mRNA guanylyltransferase activity
GO:0003723  F:RNA binding
GO:0004651  F:polynucleotide 5'-phosphatase activity
GO:0005525  F:GTP binding
GO:0004482  F:mRNA (guanine-N7-)-methyltransferase activity

Subcellular Location

From MSLVP

Single-Pass Membrane

Topology

Length:

868

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

29.53674

Exp number, first 60 AAs:

2.95848

Total prob of N-in:

0.12947

outside

1 - 802

TMhelix

803 - 825

inside

826 - 868

Population Genetic Test Statistics

Pi

0.0290969

Theta

0.038686

Tajima's D

-0.8818137

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62782.1
China_AnhuiXCGQ_2018	Protein	AYW34072.1
China_ASFV-SY18_2018	Protein	AKO62782.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95875.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10200.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10200.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10200.1
Poland_Pol17_03029_C201_2016-2017	Protein	CAN10200.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96158.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10200.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10200.1
Uganda_R8_2015	Protein	AXB49329.1
Uganda_R7_2015	Protein	AXB49503.1
Uganda_R25_2015	Protein	AXB49675.1
Uganda_N10_2015	Protein	AXB49846.1
Uganda_R35_2015	Protein	AXB50019.1
Estonia_2014	Protein	SPS73524.1
Italy_26544_OG10_2010	Protein	AJZ77049.1
Italy_47_Ss_2008_2008	Protein	AOO54458.1
Portugal_OURT_88.3_1988	Protein	CAN10450.1
Spain_E75_1975	Protein	CBH29201.1
Russia_Georgia_2007	Protein	CBW46767.1
Kenya_Tk1_2005	Protein	AJL34114.1
Kenya_Bus_2006	Protein	AJL34279.1
Russia_Odintsovo_2014	Protein	CAN10200.1
Portugal_L60_1960	Protein	AIY22292.1
Portugal_NHV_1968	Protein	AIY22450.1
Russia_Kashino_2013	Protein	CAN10200.1
Benin_1997	Protein Protein	CAN10200.1 CAN10200.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10200.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10200.1
Malawi_Lil_1983	Protein	CAN10200.1
Kenya_1950	Protein	CAN10200.1
Namibia_Warthog_1980	Protein	CAN10200.1
South_Africa_Warmbaths_1987	Protein	CAN10200.1
Malawi_Tengani_1962	Protein	CAN10200.1
China_HLJ_2018	Protein	QBH90589.1
China_LN_2018	Protein	QBH90774.1
China_wbBS01_2018	Protein	QDL88132.1
Ukraine_Kyiv_2014	Protein	QED21701.1
Belgium_Etalle_wb_2018	Protein	QED90544.1
Lithuania_LT14_2014	Protein	QEY87907.1
South_Africa_1985	Protein	QGM12928.1
South_Africa_2_2008	Protein	QGM12854.1
Zambia_LIV_1983

Sequence