Strain

South_Africa_KNP_Pretorisuskop_1996 (Region: South Africa, Kruger National Park;  Strain: Pretorisuskop/96/4;  Date: 1996)

Gene

Guanylyltransferase

Description

Annoated by sequence mapping,  structural protein

Location

AY261363.1(+):135753-138359

GenBank Accession

CAN10200.1

Full name

Probable mRNA-capping enzyme      

CDS

ATGGCTTCTTTAGATAATTTAGTGGCACGATATCAGAGGTGCTTTAATGACCAGTCTCTCAAAAATAGTACTATTGAACTTGAAATACGTTTTCAACAGATAAATTTTTTATTATTCAAAACCGTATATGAGGCACTTGTGGCACAAGAGATCCCTAGCACCATCTCCCACAGCATCCGTTGCATCAAAAAAGTTCACCATGAAAACCACTGCCGGGAAAAAATTTTGCCGTCGGAGAATTTTTACTTCAAAAAACAGCCTCTCATGTTTTTTAAGTTTTCAGAGCCTGCATCTCTGGGCTGTAAGGTCTCGCTGGCCATCGAGCAGCCCATTCGTAAATTTATCTTGGACTCCTCCGTTCTCGTTCGGCTCAAAAATCGTACGACCTTCCGGGTATCTGAACTTTGGAAAATAGAGCTTACCATTGTAAAGCAGCTGATGGGAAGCGAGGTCTCTGCAAAACTTACCGCCTTCAAAACGCTCCTGTTTGACACCCCAGAGCAACAAACGACAAAAAATATGATGTCGTTAATAAACCCAGATGACGAATATCTTTATGAAATAGAAATAGAGTATACAGGAAAGCCCGAATCCCTAACGGCGGCAGATGTTATAAAAATTAAAAACACGGTGTTGACACTTATTTCTCCGAACCATTTAATGCTAACAGCCTACCACCAGGCCATTGAATTCATTGCCTCCCATATACTGTCCTCAGAAATCCTTCTTGCACGTATTAAGAGCGGGAAGTGGGGGCTTAAACGCCTCCTTCCCCAGGTGAAATCCATGACCAAAGCGGATTACATGAAATTTTATCCGCCTGTTGGTTACTATATAACGGACAAAGCAGATGGAATTAGAGGCATCGCCGTCATTCAGGACACACAAATTTATGTGGTTGCAGACCAGCTTTACAGCCTAGGTACCACCGGCATTGAACCCCTTAAACCAACCATTTTGGACGGTGAATTTATGCCTGAAAAAAAAGAATTTTATGGGTTTGATGTCATCATGTATGAGGGCAATCTATTGACGCAACAGGGGTTTGAAACAAGAATTGAGACTTTAAGCAAGGGCATTAAAGTCTTACAAGCGTTTAACATAAAAGCAGAAATGAAGCCCTTTATTTCGCTAACAAGTGCAGATCCCAACGTGCTCCTCAAAAACTTTGAAAGCATTTTTAAGAAAAAAACTCGCCCGTATTCTATTGATGGCATCATTTTAGTAGAACCTGGCAATTCTTATCTAAATACAAACACTTTTAAGTGGAAGCCCACCTGGGATAACACATTAGACTTTTTGGTGCGAAAATGTCCAGAGAGTTTAAACGTACCAGAGTACGCGCCCAAAAAAGGGTTTTCCCTGCATCTACTATTTGTAGGCATTTCCGGAGAGCTTTTCAAGAAATTAGCGCTAAATTGGTGTCCAGGATACACGAAACTATTCCCCGTTACACAGCGCAACCAAAACTACTTTCCAGTACAGTTCCAGCCATCGGATTTTCCATTGGCATTTCTTTATTACCACCCAGATACCTCGTCATTTTCTGATATAGATGGAAAGGTCCTTGAAATGCGTTGTCTTAAGAGAGAAATCAATCACGTCAGCTGGGAAATTGTAAAAATCCGGGAGGATAGGCAGCAGGATCTTAAAACCGGCGGGTATTTTGGCAATGATTTCAAAACAGCCGAACTTACATGGCTTAACTATATGGATCCCTTTTCCTTTGAGGAGCTGGCAAAGGGCCCCTCTGGAATGTACTTCGCCGGTGCCAAAACCGGCATATACCGCGCTCAAACAGCACTTATTTCCTTTATTAAGCAAGAAATCATCCAAAAAATAAGTCACCAATCCTGGGTTATCGATCTTGGAATAGGAAAGGGGCAGGACCTAGGACGTTACTTGGACGCAGGGGTAAGGCATCTTGTTGGGATCGATAAGGATCAAACCGCGCTCGCGGAGCTTGTTTATCGAAAGTTTTCGCATGCTACAACCCGACAGCACAAGCACGCTACCAACATTTATGTGTTGCATCAAGACCTCGCAGAGCCTGCGAAGGAAATCAGCGAAAAGGTACACCAAATTTACGGGTTTCCCAAGGAGGGAGCTTCTTCCATTGTTAGCAACCTGTTTATTCACTATCTTATGAAAAACACGCAGCAGGTGGAAAACCTGGCCGTTCTGTGCCATAAGCTTCTTCAGCCGGGGGGAATGGTGTGGTTTACCACCATGCTGGGAGAACGGGTCTTAGAATTACTTCATGAAAATAGAATAGAGCTCAATGAAGTATGGGAAGCACGTGAAAACGAGGTGGTCAAATTTGCTATTAAACGTCTCTTTAAAGAGGATATATTACAGGAAACTGGGCAAGAAATTGGAGTCTTGTTACCCTTCAGCAATGGCGACTTCTACAATGAGTATCTTGTGAACACGGCGTTTTTAATTAAAATATTTAAACATCACGGATTTTCCCTGGTCCAAAAGCAGTCCTTTAAGGACTGGATTCCAGAATTTCAAAACTTTAGTAAAAGTTTGTATAAAATTCTTACAGAAGCCGATAAAACTTGGACAAGCCTTTTTGGGTTTATTTGTCTGCGCAAAAATTAA

Protein

MASLDNLVARYQRCFNDQSLKNSTIELEIRFQQINFLLFKTVYEALVAQEIPSTISHSIRCIKKVHHENHCREKILPSENFYFKKQPLMFFKFSEPASLGCKVSLAIEQPIRKFILDSSVLVRLKNRTTFRVSELWKIELTIVKQLMGSEVSAKLTAFKTLLFDTPEQQTTKNMMSLINPDDEYLYEIEIEYTGKPESLTAADVIKIKNTVLTLISPNHLMLTAYHQAIEFIASHILSSEILLARIKSGKWGLKRLLPQVKSMTKADYMKFYPPVGYYITDKADGIRGIAVIQDTQIYVVADQLYSLGTTGIEPLKPTILDGEFMPEKKEFYGFDVIMYEGNLLTQQGFETRIETLSKGIKVLQAFNIKAEMKPFISLTSADPNVLLKNFESIFKKKTRPYSIDGIILVEPGNSYLNTNTFKWKPTWDNTLDFLVRKCPESLNVPEYAPKKGFSLHLLFVGISGELFKKLALNWCPGYTKLFPVTQRNQNYFPVQFQPSDFPLAFLYYHPDTSSFSDIDGKVLEMRCLKREINHVSWEIVKIREDRQQDLKTGGYFGNDFKTAELTWLNYMDPFSFEELAKGPSGMYFAGAKTGIYRAQTALISFIKQEIIQKISHQSWVIDLGIGKGQDLGRYLDAGVRHLVGIDKDQTALAELVYRKFSHATTRQHKHATNIYVLHQDLAEPAKEISEKVHQIYGFPKEGASSIVSNLFIHYLMKNTQQVENLAVLCHKLLQPGGMVWFTTMLGERVLELLHENRIELNEVWEARENEVVKFAIKRLFKEDILQETGQEIGVLLPFSNGDFYNEYLVNTAFLIKIFKHHGFSLVQKQSFKDWIPEFQNFSKSLYKILTEADKTWTSLFGFICLRKNX

Function

Catalyzes the second reaction in the mRNA cap formation pathway.

Catalytic Activity

a 5'-triphospho-(purine-ribonucleotide)-[mRNA] + H2O = a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + H(+) + phosphate
a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + GTP + H(+) = a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + diphosphate
a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-homocysteine

Similarity

In the N-terminal section; belongs to the dsDNA virus mRNA guanylyltransferase family.
In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.

Keywords

Complete proteome   GTP-binding   Hydrolase   Methyltransferase   mRNA capping   mRNA processing   Nucleotide-binding   Nucleotidyltransferase   RNA-binding   S-adenosyl-L-methionine   Transferase   Reference proteome  

Feature

chain  Probable mRNA-capping enzyme

Uniprot

A0A0N7CR23   E0WM51   A0A2X0TKL6   A9JL69   A9JM02   A0A0A1DYG7   + More
A0A0A1DXR2   A0A1C6ZXW9   P0C994   P32094   D4I5T8   P0C993   Q65216   P0C995   A0A2Z5DGW7   A0A0C5AWD7   A0A0C5AWT8  

Pubmed

26618713    21470447    18198370    25406173    8382399    11831707    + More
8021596    25645905   

EMBL

KP055815    AKO62782.1    FR682468    CBW46767.1    MG939583    MG939588    + More
LS478113    AXZ95875.1    AXZ96158.1    SPS73524.1    AM712239    CAN10200.1    AM712240    CAN10450.1    KM262845    AIY22450.1    KM262844    AIY22292.1    KM102979    KX354450    AJZ77049.1    AOO54458.1    AY261366    L07263    U18466    FN557520    CBH29201.1    AY261363    X71982    AY261361    AY261360    MH025916    MH025917    MH025918    MH025919    MH025920    AXB49329.1    AXB49503.1    AXB49675.1    AXB49846.1    AXB50019.1    KM111294    AJL34114.1    KM111295    AJL34279.1   

Proteomes

UP000241813    UP000141072    UP000130745    UP000108903    UP000110401    UP000142390    + More
UP000117635    UP000266411    UP000000858    UP000000624    UP000101090    UP000000859    UP000000860    UP000000861    UP000105860    UP000101566   

PRIDE

P0C995   

Pfam

PF01331   mRNA_cap_enzyme
PF03291   Pox_MCEL

Interpro

IPR029063   SAM-dependent_MTases
IPR004971   mRNA_G-N7_MeTrfase_dom
IPR033469   CYTH-like_dom_sf
IPR001339   mRNA_cap_enzyme

SUPFAM

SSF53335   SSF53335
SSF55154   SSF55154

ProteinModelPortal

A0A0N7CR23   E0WM51   A0A2X0TKL6   A9JL69   A9JM02   A0A0A1DYG7   + More
A0A0A1DXR2   A0A1C6ZXW9   P0C994   P32094   D4I5T8   P0C993   Q65216   P0C995   A0A2Z5DGW7   A0A0C5AWD7   A0A0C5AWT8  

PDB

2HV9     E-value=6.37495e-06,     Score=123