Strain

China_HLJ_2018 (Region: China, Heilongjiang; Strain: Pig/HLJ/2018; Date: 2018)

Gene

I215L

Description

Annotated in NCBI, I215L

Location

MK333180.1(-):173844-174482

GenBank Accession

QBH90629.1

Full name

Ubiquitin-conjugating enzyme E2

Alternative Name

E2 ubiquitin-conjugating enzyme
Ubiquitin carrier protein
Ubiquitin-protein ligase
pI215L

CDS

ATGGTTTCCAGGTTTTTAATAGCAGAGTACAGACACCTGATAGAGAATCCCTCTGAGAATTTCAAAATTTCGGTGAATGAGAAGGATATGACTGAATGGGATGTGATCTTAAGGGGCCCACCTGACACTTTTTATGAGGGAGGCTTATTCAAGGCAAAAATCGCCTTTCCTCCAGAATACCCATATGCACCACCCAGATTAACATTCACCTCTGAAATGTGGCATCCTAATATTTACTCTGATGGAAAACTATGTATCTCTATCTTACACGGAGACAATGCTGAAGAACAGGGAATGACTTGGTCTCCGGCTCAAAAGATTGATACCATTCTTCTTAGTGTAATTTCACTGCTCAATGAGCCAAATCCAGATTCTCCAGCAAATGTGGATGCAGCTAAAAGCTACCGTAAACTCTTATATAAAGAGGATTTGGAATCATATCCCATGGAAGTTAAAAGGACTGTCAAAAAATCATTAGATGAGTGTTCACCAGAAGACATAGAATATTTTAAAAATGCTGCATCCAATGTTCCACCAATACCCAGTGATGCTTATGAAGATGAATGTGAAGAAATGGAGGATGACACATATATCTTAACCTATGATGATGAGGATGAAGAAGAGGAGGATGATGAGTAA

Protein

MVSRFLIAEYRHLIENPSENFKISVNEKDMTEWDVILRGPPDTFYEGGLFKAKIAFPPEYPYAPPRLTFTSEMWHPNIYSDGKLCISILHGDNAEEQGMTWSPAQKIDTILLSVISLLNEPNPDSPANVDAAKSYRKLLYKEDLESYPMEVKRTVKKSLDECSPEDIEYFKNAASNVPPIPSDAYEDECEEMEDDTYILTYDDEDEEEEDDE

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication.

Catalytic Activity

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.

Cofactor

Mg(2+)

Biophysicochemical Properties

Optimum pH is 7.5.

Similarity

Belongs to the ubiquitin-conjugating enzyme family.

Keywords

ATP-binding Complete proteome Early protein Host cytoplasm Host nucleus Late protein Nucleotide-binding Transferase Ubl conjugation pathway Reference proteome

Feature

chain Ubiquitin-conjugating enzyme E2

Uniprot

E0WM91 A0A2X0TKQ7 P0C8G5 A0A1C6ZY40 A9JLE0 D4I5G5 + More
A0A0A1DXV4 P27949 A9JM74 A0A0A1DYL6 P0C8G4 A0A0C5AZJ5 A0A0C5BCF0 P25869 P0C8G3 A0A0L7R7L2 R7QGA4 A0A2V3INM7 M2W4X1 A0A3Q3WAH7 C3YBH3 A0A1Z5LHY2 A0A2R7X0A1 A0A3B5JY78 A0A3P9PW68 A0A1Z5KW93 G3PRH3 A9QQD2 A0A293MZB7 A0A2R5LLG4 A0A087TR10 G3PRG9 A0A3Q3XPQ4 A0A147BUI4 A0A161MYM1 A0A224XWQ0 A0A3Q2YIY5 G3PRH2 G3PRH0 A0A3Q3E2X3 A0A0N5DDP3 A0A3P8NAI1 A0A3Q3E3C8 A0A3Q2X9K1 B7P274 V5HFL8 A0A452SLT0 A0A1A8QNT6 A0A2U9C4P9 C1BHE4 A0A3S3RNF6 A0A0S7K5D8 A0A481SWL2 A0A131Z6F9 A0A484CTE4 H3DDE0 L7LX90 A0A3B1JP22 A0A3Q1C6V9 A0A3P9J7W7 A0A3P9L0F9 A0A3B3HWS4 W5UDQ9 A0A3Q2NVP0 A0A191J724 A0A3B4C3J9 A0A232FH93 A0A3Q2WC29 A0A3P8NIR9 A0A3P9DJG3 A0A023GKI2 G3MMF9 A0A023FH00 A0A1E1XB99 L7M8D0 A0A3B3DUN9 A0A224ZB71 A0A131Z3Y4 A0A131XJ08 A0A3P9IAB1 A0A3Q1FN32 A0A3Q1APN4 A0A3P8UCX7 A0A3Q1GT59 Q6DG18 A0A1A8UVT9 A0A3B3XTJ9 A0A0S7K5A4 A0A3Q3L467 A0A087XM63 A0A3B3TKH6 A0A3P9P7T1 A0A315VBT1 A0A3B3Z0H4 A0A0S7K4D2 M4AY06 A0A3B5LIX4 A0A087Y3Z2 A0A3Q2ZZI8 A0A315VQF0

EC Number

2.3.2.23

Pubmed

21470447 18198370 25406173 26618713 1309282 11831707 + More
29472632 25645905 1310934 23503846 29688518 23471408 18563158 28528879 21551351 25765539 30926861 26830274 15496914 25576852 25329095 17554307 23127152 26704851 28648823 25186727 22216098 28503490 29451363 28797301 29703783 23542700

EMBL

FR682468 CBW46807.1 MG939583 MG939588 LS478113 AXZ95912.1 + More
AXZ96195.1 SPS73564.1 AY261366 KM102979 KX354450 AJZ77128.1 AOO54513.1 AM712239 CAN10240.1 FN557520 CBH29241.1 KM262844 KP055815 AIY22332.1 AKO62822.1 M77121 U18466 AM712240 CAN10490.1 KM262845 AIY22490.1 AY261363 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34317.1 AXB49369.1 AXB49543.1 AXB49715.1 AXB49886.1 AXB50059.1 KM111294 AJL34153.1 X62440 X71982 AY261361 AY261360 KQ414638 KOC66862.1 HG001780 CDF36445.1 NBIV01000115 PXF43686.1 KB454497 EME30786.1 GG666497 EEN62330.1 GFJQ02000346 JAW06624.1 KK856214 PTY25219.1 GFJQ02007594 JAV99375.1 EU247218 ABX75517.1 GFWV01019942 MAA44670.1 GGLE01006244 MBY10370.1 KK116364 KFM67549.1 GEGO01001212 JAR94192.1 GEMB01003945 JAR99311.1 GFTR01003977 JAW12449.1 ABJB010215757 ABJB010558256 ABJB010573273 ABJB010800097 ABJB011088916 ABJB011107550 DS620720 EEC00696.1 GADI01004051 GANP01008144 GEFM01005447 JAA69757.1 JAB76324.1 JAP70349.1 HAEG01013519 SBR95201.1 CP026254 AWP11053.1 BT074023 ACO08447.1 NCKU01007799 RWS02373.1 GBYX01220595 JAO72423.1 MH602729 QBH72974.1 GEDV01002541 JAP86016.1 SCKG01000012 TDH05555.1 GACK01008837 JAA56197.1 JT412038 AHH39704.1 KP938524 ALK16252.1 NNAY01000250 OXU29707.1 GBBM01002133 JAC33285.1 JO843060 AEO34677.1 GBBK01003306 JAC21176.1 GFAC01002668 JAT96520.1 GACK01005606 JAA59428.1 GFPF01013098 MAA24244.1 GEDV01002540 JAP86017.1 GEFH01002219 JAP66362.1 BC076537 AAH76537.1 HAEJ01010990 SBS51447.1 GBYX01220589 JAO72424.1 AYCK01020009 NHOQ01001926 PWA20853.1 GBYX01220596 JAO72422.1 AYCK01014032 NHOQ01001318 PWA25391.1

Proteomes

UP000141072 UP000000858 UP000117635 UP000266411 UP000130745 UP000101090 + More
UP000142390 UP000241813 UP000000624 UP000108903 UP000110401 UP000000859 UP000101566 UP000105860 UP000000860 UP000000861 UP000053825 UP000012073 UP000247409 UP000030680 UP000261620 UP000001554 UP000005226 UP000007635 UP000054359 UP000264820 UP000261660 UP000046395 UP000001555 UP000291022 UP000246464 UP000285301 UP000007303 UP000018467 UP000257160 UP000001038 UP000221080 UP000265000 UP000261440 UP000264840 UP000261560 UP000257200 UP000261480 UP000261640 UP000028760 UP000261500 UP000002852 UP000261380 UP000264800

Pfam

PF00179 UQ_con

Interpro

IPR016135   UBQ-conjugating_enzyme/RWD
IPR023313   UBQ-conjugating_AS
IPR000608   UBQ-conjugat_E2

SUPFAM

SSF54495 SSF54495

Gene 3D

3.10.110.10

CDD

cd00195 UBCc

ProteinModelPortal

E0WM91 A0A2X0TKQ7 P0C8G5 A0A1C6ZY40 A9JLE0 D4I5G5 + More
A0A0A1DXV4 P27949 A9JM74 A0A0A1DYL6 P0C8G4 A0A0C5AZJ5 A0A0C5BCF0 P25869 P0C8G3 A0A0L7R7L2 R7QGA4 A0A2V3INM7 M2W4X1 A0A3Q3WAH7 C3YBH3 A0A1Z5LHY2 A0A2R7X0A1 A0A3B5JY78 A0A3P9PW68 A0A1Z5KW93 G3PRH3 A9QQD2 A0A293MZB7 A0A2R5LLG4 A0A087TR10 G3PRG9 A0A3Q3XPQ4 A0A147BUI4 A0A161MYM1 A0A224XWQ0 A0A3Q2YIY5 G3PRH2 G3PRH0 A0A3Q3E2X3 A0A0N5DDP3 A0A3P8NAI1 A0A3Q3E3C8 A0A3Q2X9K1 B7P274 V5HFL8 A0A452SLT0 A0A1A8QNT6 A0A2U9C4P9 C1BHE4 A0A3S3RNF6 A0A0S7K5D8 A0A481SWL2 A0A131Z6F9 A0A484CTE4 H3DDE0 L7LX90 A0A3B1JP22 A0A3Q1C6V9 A0A3P9J7W7 A0A3P9L0F9 A0A3B3HWS4 W5UDQ9 A0A3Q2NVP0 A0A191J724 A0A3B4C3J9 A0A232FH93 A0A3Q2WC29 A0A3P8NIR9 A0A3P9DJG3 A0A023GKI2 G3MMF9 A0A023FH00 A0A1E1XB99 L7M8D0 A0A3B3DUN9 A0A224ZB71 A0A131Z3Y4 A0A131XJ08 A0A3P9IAB1 A0A3Q1FN32 A0A3Q1APN4 A0A3P8UCX7 A0A3Q1GT59 Q6DG18 A0A1A8UVT9 A0A3B3XTJ9 A0A0S7K5A4 A0A3Q3L467 A0A087XM63 A0A3B3TKH6 A0A3P9P7T1 A0A315VBT1 A0A3B3Z0H4 A0A0S7K4D2 M4AY06 A0A3B5LIX4 A0A087Y3Z2 A0A3Q2ZZI8 A0A315VQF0

PDB

2CYX E-value=6.01097e-35, Score=366

Ontologies

KEGG

vg:22220370

GO

GO:0016567  P:protein ubiquitination
GO:0042025  C:host cell nucleus
GO:0005524  F:ATP binding
GO:0030430  C:host cell cytoplasm
GO:0016740  F:transferase activity
GO:0016874  F:ligase activity
GO:0061631  F:ubiquitin conjugating enzyme activity
GO:0000209  P:protein polyubiquitination
GO:0043161  P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0006511  P:ubiquitin-dependent protein catabolic process

Subcellular Location

From MSLVP

Cytoplasm

From Uniprot

Host cytoplasm Accumulates in cytoplasmic viral factories. Faintly detected in the nucleus. With evidence from 1 publications.
Host nucleus Accumulates in cytoplasmic viral factories. Faintly detected in the nucleus. With evidence from 1 publications.

Topology

Length:

212

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0

Exp number, first 60 AAs:

0

Total prob of N-in:

0.04002

outside

1 - 212

Population Genetic Test Statistics

Pi

0.0564953

Theta

0.0648525

Tajima's D

-0.4543998

CLR

0.182016

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62822.1
China_AnhuiXCGQ_2018	Protein	AYW34112.1
China_ASFV-SY18_2018	Protein	AKO62822.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95912.1
Poland_Pol16_20538_o9_2016-2017
Poland_Pol16_20540_o10_2016-2017
Poland_Pol16_29413_o23_2016-2017
Poland_Pol17_03029_C201_2016-2017
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96195.1
Poland_Pol17_05838_C220_2016-2017
Poland_ASFV_POL_Podlaskie_2015
Uganda_R8_2015	Protein	AXB49369.1
Uganda_R7_2015	Protein	AXB49543.1
Uganda_R25_2015	Protein	AXB49715.1
Uganda_N10_2015	Protein	AXB49886.1
Uganda_R35_2015	Protein	AXB50059.1
Estonia_2014	Protein	SPS73564.1
Italy_26544_OG10_2010	Protein	AJZ77128.1
Italy_47_Ss_2008_2008	Protein	AOO54513.1
Portugal_OURT_88.3_1988	Protein	CAN10490.1
Spain_E75_1975	Protein	CBH29241.1
Russia_Georgia_2007	Protein	CBW46807.1
Kenya_Tk1_2005	Protein	AJL34153.1
Kenya_Bus_2006	Protein Protein	AJL34317.1 AJL34317.1
Russia_Odintsovo_2014
Portugal_L60_1960	Protein	AIY22332.1
Portugal_NHV_1968	Protein	AIY22490.1
Russia_Kashino_2013
Benin_1997	Protein	CAN10240.1
South_Africa_KNP_Pretorisuskop_1996	Protein	AJL34317.1
South_Africa_MGR_Mkuzi_1979
Malawi_Lil_1983
Kenya_1950	Protein	AJL34317.1
Namibia_Warthog_1980
South_Africa_Warmbaths_1987
Malawi_Tengani_1962
China_HLJ_2018	Protein	QBH90629.1
China_LN_2018	Protein	QBH90814.1
China_wbBS01_2018	Protein	QDL88172.1
Ukraine_Kyiv_2014	Protein	QED21757.1
Belgium_Etalle_wb_2018	Protein	QED90584.1
Lithuania_LT14_2014	Protein	QEY87947.1
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence