Strain

Kenya_1950 (Region: Kenya ;  Strain: Kenya 1950;  Date: 1950)

Gene

BA71V-I215L (k13L)

Description

With frameshift mutation but translatable,  similar to African swine fever virus E75 strain E75 of INSD accession FN557520

Location

AY261360.1(-):178161-178817

GenBank Accession

AJL34317.1

Full name

Ubiquitin-conjugating enzyme E2      

Alternative Name

E2 ubiquitin-conjugating enzyme
Ubiquitin carrier protein
Ubiquitin-protein ligase
pI215L

CDS

ATGGTTTCCAGATTTTTACTGACAGAGTACAGAAACCTAACAGAGAATCCTTCTGAACATTTCAAAGTCTCAGTGAATGAGAATAATCTAACTGAATGGGATGTCATCTTAAAGGGTCCATCTGACACTTTTTATGAGGGAGGCTTATTCAAGGCAAAGATCACCTTTCCTCCAAATTATCCATATGAACCACCCAAATTAACATTCACCTCTGAAATGTGGCATCCTAATATCTATCCTGATGGGAGACTATGCATCTCTATCTTACACACAGACAATGTTGAGGAAAGGGGAATGACTTGGTCTCCAGCTCAAAAGATTGAAACTGTTCTTCTTAGTGTAATCTCACTGTTCAATGAGCCAAATACGGATTCTCCAGCAAATGTAGATGCGGCCAAAAGCTACCGTAAATTGATGCATAAAGAGGATTTGGAATCATACCCCATGGAAGTTAAAAAGACTGTCAAAAAATCATTAGATGAGTGTTCAGCAGAAGATATAGAATATTTTAAAAATGCTGTATTCAATGTTCCAGCCATTCCCAGTGATGCTTATGAAGATGAACATGAAGATGAAGAAATGGAAGATGGCACATATATCTTAACCTATGATGATGAGGATGAAGAAGAGGAGGATGATGAGATGGATGATGAGTAA

Protein

MVSRFLLTEYRNLTENPSEHFKVSVNENNLTEWDVILKGPSDTFYEGGLFKAKITFPPNYPYEPPKLTFTSEMWHPNIYPDGRLCISILHTDNVEERGMTWSPAQKIETVLLSVISLFNEPNTDSPANVDAAKSYRKLMHKEDLESYPMEVKKTVKKSLDECSAEDIEYFKNAVFNVPAIPSDAYEDEHEDEEMEDGTYILTYDDEDEEEEDDEMDDE

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication.

Catalytic Activity

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.

Cofactor

Mg(2+)

Biophysicochemical Properties

Optimum pH is 7.5.

Similarity

Belongs to the ubiquitin-conjugating enzyme family.

Keywords

ATP-binding   Complete proteome   Early protein   Host cytoplasm   Host nucleus   Late protein   Nucleotide-binding   Transferase   Ubl conjugation pathway   Reference proteome  

Feature

chain  Ubiquitin-conjugating enzyme E2

Uniprot

A0A0C5AZJ5   A0A0C5BCF0   P0C8G3   P0C8G4   A0A1C6ZY40   A9JLE0   + More
D4I5G5   A0A0A1DXV4   P27949   A9JM74   A0A0A1DYL6   E0WM91   A0A2X0TKQ7   P0C8G5   P25869   A0A0L7R7L2   A0A1Z5LHY2   A0A1Z5KW93   A0A2R5LLG4   A0A293MZB7   A0A0D7BGI5   A0A087TR10   A0A147BUI4   A9QQD2   B7P274   V5HFL8   K5VP26   A0A0D0BHT8   A0A131Z6F9   L7LX90   A0A165SCC1   A0A166A000   J3JTL0   A0A0L0HLC1   A0A023GKI2   G3MMF9   A0A023FH00   A0A1E1XB99   L7M8D0   A0A224ZB71   A0A131Z3Y4   A0A131XJ08   S4PDQ4   A0A0T6B5F6   V5H1B7   A0A1B7N0Q2   A0A077YYV0   A0A2S2NNN7   A0A1J8Q2W2   T1IWR4   A0A2R7X0A1   A0A2B4S474   R7QGA4   A0A165DMV0   A0A2V3INM7   A0A161MYM1   A0A2H8TXE0   C3YBH3   C4WY13   A0A224XWQ0   A0A0D0DPY1   A0A2S2R7I8   A0A328EEQ5   M2PGB5   A0A034W735   A0A0P8XUM2   A0A0A1X533   A0A0P8ZV15   Q6NN25   Q2M1D5   W8C2V6   A0A0P8YAE9   B3MAC8   A0A3B0L036   A0A1W4VAW8   A0A1W4VPA1   B9HMG0   A0A0J9RX02   A0A2L2YIS9   M2W4X1   Q6AWG5   A0A1W4VMX7   A0A0R3SDC9   A0A0J9RVP3   Q8IQM5   A0A0J9RW33   Q9VUR4   A0A212EKI5   A0A0L0CEC3   B4L0I7   A0A0N5DDP3   A0A0Q5UJZ3   A0A0Q5U4V9   A0A286XST4   A0A0R1DYI9   A0A0Q9XFQ3   S7Q6S8   B4N4V9   A0A1I7Y1F2   A0A0R1E483  

EC Number

2.3.2.23

Pubmed

25645905    18198370    25406173    26618713    1309282    11831707    + More
29472632    21470447    1310934    28528879    25683379    25765539    23045686    26830274    25576852    26659563    22516182    23537049    22216098    28503490    28797301    23622113    23503846    29688518    18563158    22434909    25348373    17994087    18057021    25830018    15632085    24495485    16973872    22936249    26561354    23471408    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    22118469    26108605    21993624    17550304    22745431    26392177   

EMBL

KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    + More
AJL34317.1    AXB49369.1    AXB49543.1    AXB49715.1    AXB49886.1    AXB50059.1    KM111294    AJL34153.1    AY261360    AY261363    KM102979    KX354450    AJZ77128.1    AOO54513.1    AM712239    CAN10240.1    FN557520    CBH29241.1    KM262844    KP055815    AIY22332.1    AKO62822.1    M77121    U18466    AM712240    CAN10490.1    KM262845    AIY22490.1    FR682468    CBW46807.1    MG939583    MG939588    LS478113    AXZ95912.1    AXZ96195.1    SPS73564.1    AY261366    X62440    X71982    AY261361    KQ414638    KOC66862.1    GFJQ02000346    JAW06624.1    GFJQ02007594    JAV99375.1    GGLE01006244    MBY10370.1    GFWV01019942    MAA44670.1    KN880481    KIY69582.1    KK116364    KFM67549.1    GEGO01001212    JAR94192.1    EU247218    ABX75517.1    ABJB010215757    ABJB010558256    ABJB010573273    ABJB010800097    ABJB011088916    ABJB011107550    DS620720    EEC00696.1    GADI01004051    GANP01008144    GEFM01005447    JAA69757.1    JAB76324.1    JAP70349.1    JH971404    EKM76214.1    KN835169    KIK45642.1    GEDV01002541    JAP86016.1    GACK01008837    JAA56197.1    KV425574    KZT24952.1    KV426130    KZV87299.1    APGK01059412    APGK01059413    BT126567    KB741293    KB632046    AEE61531.1    ENN70240.1    ERL88251.1    KQ257454    KND01599.1    GBBM01002133    JAC33285.1    JO843060    AEO34677.1    GBBK01003306    JAC21176.1    GFAC01002668    JAT96520.1    GACK01005606    JAA59428.1    GFPF01013098    MAA24244.1    GEDV01002540    JAP86017.1    GEFH01002219    JAP66362.1    GAIX01007395    JAA85165.1    LJIG01009780    KRT82395.1    GALX01000454    JAB68012.1    KV448296    OAX38426.1    HG805835    CDW52934.1    GGMR01006190    MBY18809.1    LVVM01003391    OJA15015.1    JH431629    KK856214    PTY25219.1    LSMT01000169    PFX24701.1    HG001780    CDF36445.1    KV427631    KZT05227.1    NBIV01000115    PXF43686.1    GEMB01003945    JAR99311.1    GFXV01006765    MBW18570.1    GG666497    EEN62330.1    ABLF02032289    AK342968    BAH72783.1    GFTR01003977    JAW12449.1    KN825125    KIK94173.1    GGMS01016457    MBY85660.1    NQVE01000005    RAL54861.1    KB445801    EMD35024.1    GAKP01009002    GAKP01009001    JAC49950.1    CH902618    KPU78428.1    KPU78429.1    GBXI01007858    JAD06434.1    KPU78426.1    BT011473    AAR99131.1    CH379069    EAL30639.3    GAMC01006370    GAMC01006369    JAC00187.1    KPU78427.1    EDV40179.2    OUUW01000027    SPP89738.1    CM009297    PNT22894.1    CM002912    KMY99784.1    IAAA01026973    LAA07937.1    KB454497    EME30786.1    BT015283    AAT94512.1    UYSG01000693    VDL20895.1    KMY99783.1    AE014296    AAN11776.1    AGB94578.1    KMY99782.1    BT100166    AAF49611.2    ACY07071.1    AGBW02014274    OWR41997.1    JRES01000502    KNC30596.1    CH933809    EDW19156.2    CH954178    KQS44112.1    KQS44111.1    AAKN02057514    CM000159    KRK02102.1    KRK02109.1    KRG06521.1    KB469301    EPQ55751.1    CH964101    EDW79398.1    KRK02103.1    KRK02106.1   

Proteomes

UP000101566    UP000105860    UP000000861    UP000000859    UP000117635    UP000266411    + More
UP000130745    UP000101090    UP000142390    UP000241813    UP000000624    UP000108903    UP000110401    UP000141072    UP000000858    UP000000860    UP000053825    UP000054007    UP000054359    UP000001555    UP000008493    UP000054485    UP000076761    UP000077266    UP000019118    UP000030742    UP000053201    UP000092154    UP000183567    UP000014500    UP000225706    UP000012073    UP000076871    UP000247409    UP000001554    UP000007819    UP000054538    UP000016930    UP000007801    UP000001819    UP000192221    UP000006729    UP000030680    UP000046397    UP000000803    UP000007151    UP000037069    UP000009192    UP000046395    UP000008711    UP000005447    UP000002282    UP000030669    UP000007798    UP000095287   

PRIDE

Q6NN25   

Pfam

PF00179   UQ_con

Interpro

IPR016135   UBQ-conjugating_enzyme/RWD
IPR023313   UBQ-conjugating_AS
IPR000608   UBQ-conjugat_E2

SUPFAM

SSF54495   SSF54495

Gene 3D

3.10.110.10    

CDD

cd00195  UBCc

ProteinModelPortal

A0A0C5AZJ5   A0A0C5BCF0   P0C8G3   P0C8G4   A0A1C6ZY40   A9JLE0   + More
D4I5G5   A0A0A1DXV4   P27949   A9JM74   A0A0A1DYL6   E0WM91   A0A2X0TKQ7   P0C8G5   P25869   A0A0L7R7L2   A0A1Z5LHY2   A0A1Z5KW93   A0A2R5LLG4   A0A293MZB7   A0A0D7BGI5   A0A087TR10   A0A147BUI4   A9QQD2   B7P274   V5HFL8   K5VP26   A0A0D0BHT8   A0A131Z6F9   L7LX90   A0A165SCC1   A0A166A000   J3JTL0   A0A0L0HLC1   A0A023GKI2   G3MMF9   A0A023FH00   A0A1E1XB99   L7M8D0   A0A224ZB71   A0A131Z3Y4   A0A131XJ08   S4PDQ4   A0A0T6B5F6   V5H1B7   A0A1B7N0Q2   A0A077YYV0   A0A2S2NNN7   A0A1J8Q2W2   T1IWR4   A0A2R7X0A1   A0A2B4S474   R7QGA4   A0A165DMV0   A0A2V3INM7   A0A161MYM1   A0A2H8TXE0   C3YBH3   C4WY13   A0A224XWQ0   A0A0D0DPY1   A0A2S2R7I8   A0A328EEQ5   M2PGB5   A0A034W735   A0A0P8XUM2   A0A0A1X533   A0A0P8ZV15   Q6NN25   Q2M1D5   W8C2V6   A0A0P8YAE9   B3MAC8   A0A3B0L036   A0A1W4VAW8   A0A1W4VPA1   B9HMG0   A0A0J9RX02   A0A2L2YIS9   M2W4X1   Q6AWG5   A0A1W4VMX7   A0A0R3SDC9   A0A0J9RVP3   Q8IQM5   A0A0J9RW33   Q9VUR4   A0A212EKI5   A0A0L0CEC3   B4L0I7   A0A0N5DDP3   A0A0Q5UJZ3   A0A0Q5U4V9   A0A286XST4   A0A0R1DYI9   A0A0Q9XFQ3   S7Q6S8   B4N4V9   A0A1I7Y1F2   A0A0R1E483  

PDB

2CYX     E-value=6.01097e-35,     Score=366