Strain

Benin_1997 (Region: Benin;  Strain: Benin 97/1;  Date: 1997)

Gene

ubiquitin-conjugating enzyme

Description

Annotated in NCBI,  gene expression: immediate-early

Location

AM712239.1(-):168429-169076

GenBank Accession

CAN10240.1

Full name

Ubiquitin-conjugating enzyme E2      

Alternative Name

E2 ubiquitin-conjugating enzyme
Ubiquitin carrier protein
Ubiquitin-protein ligase
pI215L

CDS

ATGGTTTCCAGGTTTTTAATAGCAGAGTACAGACACCTGATAGAGAACCCCTCTGAGAATTTCAAAATTTCAGTGAATGAGAATAATATAACTGAATGGGATGTGATCTTAAGGGGCCCACCTGACACTCTTTATGAGGGAGGCTTATTCAAGGCAAAAGTCGCCTTTCCTCCAGAATACCCATATGCACCACCCAAATTAACATTCACCTCTGAAATGTGGCATCCTAATATTTACCCTGATGGAAGACTATGTATCTCTATCTTACACGGAGACAATGCTGAAGAACAGGGAATGACTTGGTCTCCGGCTCAAAAGATTGATACCATTCTTCTTAGTGTAATTTCATTGCTCAATGAGCCAAATCCAGATTCTCCAGCAAATGTGGATGCAGCTAAAAGCTACCGTAAATATGTATATAAAGAGGATTTGGAATCATATCCCATGGAAGTTAAAAAGACTGTCAAAAAATCATTAGATGAGTGTTCACCAGAAGACATAGAATATTTTAAAAATGCTGCATCCAATGTTCCACCAATACCCAGTGATGCTTATGAAGATGAATGTGAAGAAATGGAGGATGACACATATATCTTAACCTATGATGATGATGAAGAAGAGGAGGATGAAGAGATGGATGATGAGTAA

Protein

MVSRFLIAEYRHLIENPSENFKISVNENNITEWDVILRGPPDTLYEGGLFKAKVAFPPEYPYAPPKLTFTSEMWHPNIYPDGRLCISILHGDNAEEQGMTWSPAQKIDTILLSVISLLNEPNPDSPANVDAAKSYRKYVYKEDLESYPMEVKKTVKKSLDECSPEDIEYFKNAASNVPPIPSDAYEDECEEMEDDTYILTYDDDEEEEDEEMDDE

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome. By controlling the ubiquitination status of specific host proteins, the virus may target them to degradation and thereby optimize the viral replication.

Catalytic Activity

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.

Cofactor

Mg(2+)

Biophysicochemical Properties

Optimum pH is 7.5.

Similarity

Belongs to the ubiquitin-conjugating enzyme family.

Keywords

ATP-binding   Complete proteome   Early protein   Host cytoplasm   Host nucleus   Late protein   Nucleotide-binding   Reference proteome   Transferase   Ubl conjugation pathway  

Feature

chain  Ubiquitin-conjugating enzyme E2

Uniprot

A0A1C6ZY40   A9JM74   A0A0A1DYL6   A9JLE0   D4I5G5   A0A0A1DXV4   + More
P27949   P0C8G5   E0WM91   A0A2X0TKQ7   P0C8G4   A0A0C5BCF0   A0A0C5AZJ5   P0C8G3   P25869   A0A0L7R7L2   A0A2V3INM7   M2W4X1   A0A1Z5LHY2   A0A166A000   A0A087TR10   A0A059A8R9   A9QQD2   A0A1Z5KW93   A0A061QWU1   A0A2R5LLG4   A0A293MZB7   A0A059A7Y8   A0A397W6C0   R7QGA4   J3JTL0   A0A147BUI4   C3YBH3   B7P274   V5HFL8   L7LX90   A0A131Z6F9   A0A1Y1Y1T0   A0A2R7X0A1   A0A023GKI2   G3MMF9   A0A224ZB71   A0A131Z3Y4   A0A023FH00   A0A131XJ08   A0A1E1XB99   D8U4W4   L7M8D0   A0A1Y2GN14   A0A059AA29   A0A059A923   A0A0T6B5F6   A0A1V9XRN5   A0A0D7BGI5   V5H1B7   B9HMG0   A0A085MIH0   A0A177B2V6   A0A232FH93   A0A077Z2M4   T1IWR4   A0A2L2YIS9   M5FSG7   A0A165SCC1   A0A1V9Z888   A0A2S2NNN7   A0A161MYM1   A0A224XWQ0   A0A034W735   A0A1Y1XZI8   A8NJT4   W8C2V6   A0A0A1X533   A0A0M3QWT2   K5VME4   Q6NN25   Q2M1D5   A0A2S2R7I8   A0A0D0BHT8   A0A0L0CEC3   A0A3B0L036   A0A087SS26   A0A0J9RVP3   Q8IQM5   A0A0X3PA50   J4IA11   A0A0J9RW33   T1IP95   A0A0Q5UJZ4   A0A137R038   B3NI46   A0A166GYH0   S7Q6S8  

EC Number

2.3.2.23

Pubmed

18198370    25406173    26618713    1309282    11831707    29472632    + More
21470447    25645905    1310934    29688518    23471408    28528879    26659563    24919147    23503846    22516182    23537049    18563158    25765539    25576852    26830274    22216098    28797301    28503490    20616280    28327890    25683379    16973872    24929829    28648823    26561354    22745431    25527045    25348373    20547848    24495485    25830018    22937793    15632085    17994087    26108605    25012212    22936249    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    22247176    18057021   

EMBL

KM102979    KX354450    AJZ77128.1    AOO54513.1    AM712240    CAN10490.1    + More
KM262845    AIY22490.1    AM712239    CAN10240.1    FN557520    CBH29241.1    KM262844    KP055815    AIY22332.1    AKO62822.1    M77121    U18466    AY261366    FR682468    CBW46807.1    MG939583    MG939588    LS478113    AXZ95912.1    AXZ96195.1    SPS73564.1    AY261363    KM111294    AJL34153.1    KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    AJL34317.1    AXB49369.1    AXB49543.1    AXB49715.1    AXB49886.1    AXB50059.1    AY261360    X62440    X71982    AY261361    KQ414638    KOC66862.1    NBIV01000115    PXF43686.1    KB454497    EME30786.1    GFJQ02000346    JAW06624.1    KV426130    KZV87299.1    KK116364    KFM67549.1    KK198762    KCW50233.1    EU247218    ABX75517.1    GFJQ02007594    JAV99375.1    GBEZ01024174    GBEZ01020476    JAC62786.1    JAC66202.1    GGLE01006244    MBY10370.1    GFWV01019942    MAA44670.1    KCW50232.1    QKWP01000033    RIB29631.1    HG001780    CDF36445.1    APGK01059412    APGK01059413    BT126567    KB741293    KB632046    AEE61531.1    ENN70240.1    ERL88251.1    GEGO01001212    JAR94192.1    GG666497    EEN62330.1    ABJB010215757    ABJB010558256    ABJB010573273    ABJB010800097    ABJB011088916    ABJB011107550    DS620720    EEC00696.1    GADI01004051    GANP01008144    GEFM01005447    JAA69757.1    JAB76324.1    JAP70349.1    GACK01008837    JAA56197.1    GEDV01002541    JAP86016.1    MCFE01000299    ORX91957.1    KK856214    PTY25219.1    GBBM01002133    JAC33285.1    JO843060    AEO34677.1    GFPF01013098    MAA24244.1    GEDV01002540    JAP86017.1    GBBK01003306    JAC21176.1    GEFH01002219    JAP66362.1    GFAC01002668    JAT96520.1    GL378358    EFJ45260.1    GACK01005606    JAA59428.1    MCFF01000018    ORZ16088.1    KCW50235.1    KCW50234.1    LJIG01009780    KRT82395.1    MNPL01005239    OQR76149.1    KN880481    KIY69582.1    GALX01000454    JAB68012.1    CM009297    PNT22894.1    KL363191    KL367490    KFD57016.1    KFD70116.1    LWCA01000417    OAF68609.1    NNAY01000250    OXU29707.1    HG805835    CDW52950.1    JH431629    IAAA01026973    LAA07937.1    JH795867    EJU00376.1    KV425574    KZT24952.1    JNBR01000369    OQR94224.1    GGMR01006190    MBY18809.1    GEMB01003945    JAR99311.1    GFTR01003977    JAW12449.1    GAKP01009002    GAKP01009001    JAC49950.1    MCFE01000344    ORX91075.1    AACS02000010    EAU87530.2    GAMC01006370    GAMC01006369    JAC00187.1    GBXI01007858    JAD06434.1    CP012525    ALC44733.1    JH930475    EKM52638.1    BT011473    AAR99131.1    CH379069    EAL30639.3    GGMS01016457    MBY85660.1    KN835169    KIK45642.1    JRES01000502    KNC30596.1    OUUW01000027    SPP89738.1    GDKF01001155    KL662173    QOKY01000198    JAT77467.1    KFM28530.1    RMZ53353.1    CM002912    KMY99783.1    AE014296    AAN11776.1    AGB94578.1    GEEE01014496    JAP48729.1    HE797063    CCM02101.1    KMY99782.1    JH431251    CH954178    KQS44114.1    KQ961879    KXN92693.1    EDV52132.2    KQS44113.1    KV424509    KZV71704.1    KB469301    EPQ55751.1   

Proteomes

UP000117635    UP000266411    UP000108903    UP000110401    UP000130745    UP000101090    + More
UP000142390    UP000241813    UP000000624    UP000000858    UP000141072    UP000000859    UP000105860    UP000101566    UP000000861    UP000000860    UP000053825    UP000247409    UP000030680    UP000077266    UP000054359    UP000030711    UP000266673    UP000012073    UP000019118    UP000030742    UP000001554    UP000001555    UP000193498    UP000001058    UP000193648    UP000192247    UP000054007    UP000006729    UP000014500    UP000030653    UP000076761    UP000243579    UP000001861    UP000092553    UP000008370    UP000001819    UP000054485    UP000037069    UP000028924    UP000000803    UP000006352    UP000008711    UP000070249    UP000077086    UP000030669   

PRIDE

Q6NN25   

Pfam

PF00179   UQ_con

Interpro

IPR016135   UBQ-conjugating_enzyme/RWD
IPR000608   UBQ-conjugat_E2
IPR023313   UBQ-conjugating_AS

SUPFAM

SSF54495   SSF54495

Gene 3D

3.10.110.10    

CDD

cd00195  UBCc

ProteinModelPortal

A0A1C6ZY40   A9JM74   A0A0A1DYL6   A9JLE0   D4I5G5   A0A0A1DXV4   + More
P27949   P0C8G5   E0WM91   A0A2X0TKQ7   P0C8G4   A0A0C5BCF0   A0A0C5AZJ5   P0C8G3   P25869   A0A0L7R7L2   A0A2V3INM7   M2W4X1   A0A1Z5LHY2   A0A166A000   A0A087TR10   A0A059A8R9   A9QQD2   A0A1Z5KW93   A0A061QWU1   A0A2R5LLG4   A0A293MZB7   A0A059A7Y8   A0A397W6C0   R7QGA4   J3JTL0   A0A147BUI4   C3YBH3   B7P274   V5HFL8   L7LX90   A0A131Z6F9   A0A1Y1Y1T0   A0A2R7X0A1   A0A023GKI2   G3MMF9   A0A224ZB71   A0A131Z3Y4   A0A023FH00   A0A131XJ08   A0A1E1XB99   D8U4W4   L7M8D0   A0A1Y2GN14   A0A059AA29   A0A059A923   A0A0T6B5F6   A0A1V9XRN5   A0A0D7BGI5   V5H1B7   B9HMG0   A0A085MIH0   A0A177B2V6   A0A232FH93   A0A077Z2M4   T1IWR4   A0A2L2YIS9   M5FSG7   A0A165SCC1   A0A1V9Z888   A0A2S2NNN7   A0A161MYM1   A0A224XWQ0   A0A034W735   A0A1Y1XZI8   A8NJT4   W8C2V6   A0A0A1X533   A0A0M3QWT2   K5VME4   Q6NN25   Q2M1D5   A0A2S2R7I8   A0A0D0BHT8   A0A0L0CEC3   A0A3B0L036   A0A087SS26   A0A0J9RVP3   Q8IQM5   A0A0X3PA50   J4IA11   A0A0J9RW33   T1IP95   A0A0Q5UJZ4   A0A137R038   B3NI46   A0A166GYH0   S7Q6S8  

PDB

2CYX     E-value=6.01097e-35,     Score=366