Strain

Uganda_N10_2015 (Region: Uganda, Tororo district; Strain: N10; Date: 2015)

Gene

pB646L

Description

Annotated in NCBI, gene expression: late

Location

MH025919.1(-):102566-104506

GenBank Accession

AXB49827.1

Full name

Major capsid protein

Alternative Name

p72
p73

CDS

ATGGCATCAGGAGGAGCTTTTTGTCTTATTGCTAACGATGGGAAGGCCGACAAGATCATTTTGGCCCAAGACTTGCTTAATAGCAGGATCTCTAACATTAAAAATGTTAACAAAAGTTATGGGAAACCCGACCCCGAACCCACTTTGAGTCAAATCGAAGAAACACATATGGTGCATTTTAACGCACATTTTAAGCCTTACGTCCCAATAGGGTTTGAATACAATAAAGTACGTCCACATACGGGTACCCCCACATTGGGCAACAAGCTTACCTTTGGTATTCCCCAGTACGGAGACTTTTTCCATGATATGGTAGGCCACCATATATTGGGCGCGTGTCATTCCTCCTGGCAGGATGCTCCGATTCAGGGCTCATCCCAGATGGGGGCCCATGGTCAGCTTCAAACGTTTCCTCGCAACGGATATGACTGGGACAACCAAACACCTTTGGAGGGCGCCGTTTACACGCTTGTGGATCCTTTTGGGAGGCCCATTGTGCCCGGCACAAAGAATGCGTACCGAAACTTGGTTTACTACTGCGAATACCCCGGAGAACGGCTTTATGAAAATGTAAGATTCGATGTAAATGGAAACTCCCTGGACGAATATAGTTCGGATGTTACAACGCTTGTGCGTAAATTTTGCATCCCAGGAGATAAAATGACTGGATATAAGCACTTGGTTGGCCAGGAGGTATCGGTGGAGGGAACCAGTGGCCCTCTCCTATGCAACGTTCAAGATATGCACAAGCCGCACCAGAGCAAACCTATTCTTACCGATGAAAATGATACGCAGCGAACATGCACCCATACCAACCCGAAATTTCTTTCACAGCATTTTCCCGAGAACTCCCACAATATCCAAACAGCAGGTAAACAAGATATTACCCCTATTACGGACGCAACGTATCTGGACATAAGACGTAATGTTCATTACAGCTGTAATGGACCTCAAACCCCTAAATACTATCAGCCCCCTCTTGCGCTCTGGATTAAGCTGCGCTTTTGGTTTAATGAGAACGTGAACCTTGCTATTCCCTCGGTATCTATTCCCTTCGGCGAGCGCTTTATCACCATAAAGCTTGCATCGCAAAAGGATTTGGTGAATGAATTTCCCGGACTTTTTATCCGCCAGTCACGTTTTATACCTGGGCGCCCCAGTAGACGCAACATCCGCTTTAAACCATGGTTTATCCCAGGAGTCATTAGTGAAATCTCGCTCACGAATAATGAGCTTTACATCAATAACCTGTTTGTAACCCCTGAAATACACAATCTTTTTGTAAAACGCGTTCGATTTTCCCTGATACGTGTCCATAAAACGCAGGTGACCCACACGAACAATAACCATCACGATGAAAAACTAATGTCTGCTCTTAAATGGCCCATCGAATATATGTTTATAGGATTAAAACCCACCTGGAATATTTCCGACCAAAATCCTCATCAACACCGAGATTGGCACAAGTTCGGACATGTTGTTAACGCCATTATGCAGCCTACCCACCACGCAGAGGTAAGCTTTCAGGATAGAGATACAGCTCTTCCAGATGCATGTTCATCCATATCTGATATTACCCCCATTACTTATCCGATCACATTACCTATTATTAAAAACATTTCCGTTACTGCTCACGGTATCAATCTTATCGATAAATTTCCATCAAAGTTCTGCAGCTCTTACATACCCTTCCACTACGGAGGCAATTCGATTAAAACCCCCGACGATCCGGGCGCGATGATGATTACCTTTGCTTTGAAACCACGGGAGGAATACCAACCCAGCGGTCATATTAACGTATCCAGAGCAAGAGAATTTTATATTAGCTGGGACACAGATTATGTGGGGTCTATCACCACGGCTGATCTTGTGGTATCGGCATCCGCTATTAACTTTCTTCTTCTTCAGAATGGTTCAGCTGTGTTGCGTTACAGTACCTAA

Protein

MASGGAFCLIANDGKADKIILAQDLLNSRISNIKNVNKSYGKPDPEPTLSQIEETHMVHFNAHFKPYVPIGFEYNKVRPHTGTPTLGNKLTFGIPQYGDFFHDMVGHHILGACHSSWQDAPIQGSSQMGAHGQLQTFPRNGYDWDNQTPLEGAVYTLVDPFGRPIVPGTKNAYRNLVYYCEYPGERLYENVRFDVNGNSLDEYSSDVTTLVRKFCIPGDKMTGYKHLVGQEVSVEGTSGPLLCNVQDMHKPHQSKPILTDENDTQRTCTHTNPKFLSQHFPENSHNIQTAGKQDITPITDATYLDIRRNVHYSCNGPQTPKYYQPPLALWIKLRFWFNENVNLAIPSVSIPFGERFITIKLASQKDLVNEFPGLFIRQSRFIPGRPSRRNIRFKPWFIPGVISEISLTNNELYINNLFVTPEIHNLFVKRVRFSLIRVHKTQVTHTNNNHHDEKLMSALKWPIEYMFIGLKPTWNISDQNPHQHRDWHKFGHVVNAIMQPTHHAEVSFQDRDTALPDACSSISDITPITYPITLPIIKNISVTAHGINLIDKFPSKFCSSYIPFHYGGNSIKTPDDPGAMMITFALKPREEYQPSGHINVSRAREFYISWDTDYVGSITTADLVVSASAINFLLLQNGSAVLRYST

Function

Major capsid protein, one of the most immunogenic protein in natural infections. Plays an essential role in virion assembly.
Major capsid protein, one of the most immunogenic protein in natural infections. Plays an essential role in virion assembly (PubMed:9580160).

Subunit

Homomultimer. The membrane-bound form, but not the cytosolic one, assembles into large complexes.
Homomultimer (PubMed:9573294). The membrane-bound form, but not the cytosolic one, assembles into large complexes (PubMed:9573294).

Similarity

Belongs to the NCLDV major capsid protein family.

Keywords

Capsid protein Complete proteome Host cytoplasm Host endoplasmic reticulum Host membrane Membrane Virion Direct protein sequencing Reference proteome

Feature

chain Major capsid protein

Uniprot

D2WPI9 D2WPI3 D2WPJ1 D2WPI7 D2WPI5 Q65281 + More
A0A1B0V1B5 A0A0C5AZH0 Q5IZJ5 Q65282 Q8V9S6 Q8B389 A1EC50 E0WMM0 Q5IZK2 Q5IZI7 A9JL40 D4I5S0 A9JLW9 Q8UY56 Q5IZK1 A1EC49 Q5IZK0 P22776 Q5IZJ3 Q5IZJ6 A0A1B0VC42 A0A1B0VAK7 Q9QQ83 A0A1X6WF55 K3X3E3 A0A2D5VNF6

Pubmed

20052526 27211823 25645905 15634958 8893801 1550491 + More
21470447 30102848 18198370 25406173 26618713 2327074 11831707 8970959 9580160 9573294 10872875 29337314

EMBL

GQ427193 ADB28078.1 GQ427190 ADB28072.1 GQ427194 ADB28080.1 + More
GQ427192 ADB28076.1 GQ427191 ADB28074.1 L27498 AAA42721.1 KT795353 KT795354 KT795356 KT795358 KT795359 AMQ11000.1 AMQ11001.1 AMQ11003.1 AMQ11005.1 AMQ11006.1 KM111294 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34095.1 AJL34260.1 AXB49310.1 AXB49484.1 AXB49656.1 AXB49827.1 AXB50000.1 AY261360 AY578698 L27499 AAA42722.1 AY261361 L00966 S89966 AAN86724.1 EF121429 ABL67020.1 FR682468 CBW46748.1 AY578690 AY578691 AY578694 AY578696 AY578699 AY578701 AY578702 AY578703 AY578704 AY578707 KJ195685 MH713612 MG939583 MG939587 MG939588 LS478113 AAT84439.1 AAT84440.1 AAT84443.1 AAT84445.1 AAT84448.1 AAT84450.1 AAT84451.1 AAT84452.1 AAT84453.1 AAT84456.1 AHN94306.1 AXP99042.1 AXZ95856.1 AXZ96044.1 AXZ96139.1 SPS73505.1 AY578706 AY261366 AM712239 CAN10181.1 FN557520 CBH29183.1 AM712240 CAN10431.1 L76727 AAL40036.1 AY578692 AAT84441.1 EF121428 ABL67019.1 AY578689 AY578693 AY578695 AY578705 AY578708 KM262844 KM262845 KM102979 KP055815 KX354450 AAT84438.1 AAT84442.1 AAT84444.1 AAT84454.1 AAT84457.1 AIY22273.1 AIY22431.1 AJZ77054.1 AKO62763.1 AOO54423.1 M34142 U18466 AY578700 AAT84449.1 AY578697 AAT84446.1 KT795355 AMQ11002.1 KT795357 AMQ11004.1 AF159503 AAD49226.1 LT706986 SIP85932.1 GL376611 NYYR01000108 MAE21706.1

Proteomes

UP000101566 UP000105860 UP000000861 UP000000860 UP000141072 UP000000858 + More
UP000130745 UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 UP000241813 UP000266411 UP000000624 UP000201921 UP000019132

Pfam

PF04451 Capsid_NCLDV
PF16903 Capsid_N

Interpro

IPR016112   VP_dsDNA_II
IPR007542   MCP_C
IPR038519   MCP_C_sf
IPR031654   Capsid_N

SUPFAM

SSF49749 SSF49749

Gene 3D

2.70.9.20

ProteinModelPortal

D2WPI9 D2WPI3 D2WPJ1 D2WPI7 D2WPI5 Q65281 + More
A0A1B0V1B5 A0A0C5AZH0 Q5IZJ5 Q65282 Q8V9S6 Q8B389 A1EC50 E0WMM0 Q5IZK2 Q5IZI7 A9JL40 D4I5S0 A9JLW9 Q8UY56 Q5IZK1 A1EC49 Q5IZK0 P22776 Q5IZJ3 Q5IZJ6 A0A1B0VC42 A0A1B0VAK7 Q9QQ83 A0A1X6WF55 K3X3E3 A0A2D5VNF6

PDB

6KU9 E-value=0, Score=3433

Ontologies

KEGG

vg:22220311

GO

GO:0019028  C:viral capsid
GO:0005198  F:structural molecule activity
GO:0044164  C:host cell cytosol
GO:0044167  C:host cell endoplasmic reticulum membrane
GO:0016020  C:membrane

Subcellular Location

From MSLVP

Capsid

From Uniprot

Virion   Present in the external surfaces of intracellular virus particles.   With evidence from 1 publications.
Host endoplasmic reticulum membrane   Present in the external surfaces of intracellular virus particles.   With evidence from 1 publications.
Host cytoplasm   Present in the external surfaces of intracellular virus particles.   With evidence from 1 publications.
Host cytosol   Present in the external surfaces of intracellular virus particles.   With evidence from 1 publications.

Topology

Length:

646

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.98282

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00013

outside

1 - 646

Population Genetic Test Statistics

Pi

0.0270466

Theta

0.0352706

Tajima's D

-0.8651392

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62763.1
China_AnhuiXCGQ_2018	Protein	AYW34053.1
China_ASFV-SY18_2018	Protein	AKO62763.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95856.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10181.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10181.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10181.1
Poland_Pol17_03029_C201_2016-2017	Protein	AXZ96044.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96139.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10181.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10181.1
Uganda_R8_2015	Protein	AXB49310.1
Uganda_R7_2015	Protein	AXB49484.1
Uganda_R25_2015	Protein	AXB49656.1
Uganda_N10_2015	Protein	AXB49827.1
Uganda_R35_2015	Protein	AXB50000.1
Estonia_2014	Protein	SPS73505.1
Italy_26544_OG10_2010	Protein	AJZ77054.1
Italy_47_Ss_2008_2008	Protein	AOO54423.1
Portugal_OURT_88.3_1988	Protein	CAN10431.1
Spain_E75_1975	Protein	CBH29183.1
Russia_Georgia_2007	Protein	CBW46748.1
Kenya_Tk1_2005	Protein	AJL34095.1
Kenya_Bus_2006	Protein	AJL34260.1
Russia_Odintsovo_2014	Protein	CAN10181.1
Portugal_L60_1960	Protein	AIY22273.1
Portugal_NHV_1968	Protein	AIY22431.1
Russia_Kashino_2013	Protein	CAN10181.1
Benin_1997	Protein Protein	CAN10181.1 CAN10181.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10181.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10181.1
Malawi_Lil_1983	Protein	CAN10181.1
Kenya_1950	Protein	CAN10181.1
Namibia_Warthog_1980	Protein	CAN10181.1
South_Africa_Warmbaths_1987	Protein	CAN10181.1
Malawi_Tengani_1962	Protein	CAN10181.1
China_HLJ_2018	Protein	QBH90570.1
China_LN_2018	Protein	QBH90755.1
China_wbBS01_2018	Protein	QDL88113.1
Ukraine_Kyiv_2014	Protein Protein Protein	QED21665.1 QED21666.1 QED21668.1
Belgium_Etalle_wb_2018	Protein	QED90525.1
Lithuania_LT14_2014	Protein	QEY87888.1
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence