Strain

Uganda_R25_2015 (Region: Uganda, Tororo district; Strain: R25; Date: 2015)

Gene

pCP530R

Description

Annotated in NCBI, precursor of structural proteins p35 and p15

Location

MH025918.1(+):124547-126151

GenBank Accession

AXB49668.1

Full name

Polyprotein pp62

Alternative Name

60 kDa polyprotein
62 kDa polyprotein

CDS

ATGCCCTCTAACATGAAACAGTTTTGCAAGATTTCTGTATGGCTACAGCGGCACGATCCGGATCTATTAGAAATTATCAATAACCTATGTATGCTTGGCAATTTATCCGCGGCAAAGTACAAACACGGAGTGACCTTCATTTATCCCAAACAGGCAAAGATACGTGATGAAATAAAAAAACATGCCTATTCCAATGACCCCTCACAGGCCATAAAGACCCTAGAATCACTCATCCTTCCATTTTACATTCCCACCCCAATGGAGTTCACCGGGGAAATCGGCTCCTACACCGGAGTGAAATTAGAGGTCGAAAAAAAGGAAGCGAATAAAGTTGTTTTGAAAAATGGGGAAGCAGTCCTAATACCAGCGGCTGATTTTAAACCCTTTCCTGATCGCCGGCTAGCGGTCTGGATCATGGAGTCAGGCTCTATGCCCCTAGAAGGGCCTCCCTATAAGCGGAAAAAAGAGGGTGGAGGGAATGACCCGCCGATTTCAAAGCATATCTCGCCGTATACTCCGCGCACGCGTATTGCCATTGAGGTAGAAAAGGCCTTCGATGAATGTATGCGTCAAAACTGGTGTAGTGTCAATAATCCCTATCTTGCCAAATCGGTCTCCTTGCTGTCTTTCTTGTCGCTCAACCATCCCACCGAGTTTATTAAGGTCCTGCCGCTTATAGACTTTGACCCCTTGGTGACCTTTTATCTACTTCTTGAGCCGTATAAAACGCATGGAGATGACTTTTTAATTCCGGAAACAATTTTATTCGGCCCCACCGGATGGAACGGCACAGATCTGTATCAAAGTGCCATGCTAGAATTTAAAAAGTTTTTTACGCAGATTACCCGCCAAACCTTTATGGATATAGCCGATACGGCCACTAAGGAGGTAGATGTTCCCATATGCTACTCAGATCCTGAAACCGTACATTCCTATGCTAATCACGTGCGTACTGAAATTTTGCATCATAACATGGTAAACAAAGTTACAACGCCTAACCTGGTCGTACAGGTCTACAATGAGCTCGAGCAAACCAACACAATACGACATTACGGGCCTATTTTCCCGGAAAGTACCATCAACGCACTGCGTTTCTGGAAAAAGCTGTGGCAGGATGAACAGCGGTTTGTTATCCATGGCCTGCACCGCACGTTGATGGATCAACCCACCTATGAAACCTCTGAGTTTGCAGAGATCGTTAGAAATTTACGTTTTTCGCGTCCCGGCAATAACTATATAAACGAGCTCAATATCACAAGTCCTGCTATGTACGGCGACAAGCATACCACCGGAGATATTGCGCCCAATGATAGGTTTGCCATGTTGGTGGCCTTTATTAACAGCACTGACTTTTTATATACCGCGATTCCAGAGGAAAAGGTGGGGGGAAATGATACACAAACCGGTCCCCAAACCAGTAGCCTTACAGATCTAGTTCCAACACGGCTACACTCTTTTTTAAACCATAATCTAAGCAAACTCAAAATATTGAATCGTGCGCAGCAAACGGTTAAAAATATTCTTTCAAATGATTGTCTTAATCAACTAAAACATTATGTTAAACACACGGGAAAAAATGAAATACTAAAGATACTTCAAGAATAA

Protein

MPSNMKQFCKISVWLQRHDPDLLEIINNLCMLGNLSAAKYKHGVTFIYPKQAKIRDEIKKHAYSNDPSQAIKTLESLILPFYIPTPMEFTGEIGSYTGVKLEVEKKEANKVVLKNGEAVLIPAADFKPFPDRRLAVWIMESGSMPLEGPPYKRKKEGGGNDPPISKHISPYTPRTRIAIEVEKAFDECMRQNWCSVNNPYLAKSVSLLSFLSLNHPTEFIKVLPLIDFDPLVTFYLLLEPYKTHGDDFLIPETILFGPTGWNGTDLYQSAMLEFKKFFTQITRQTFMDIADTATKEVDVPICYSDPETVHSYANHVRTEILHHNMVNKVTTPNLVVQVYNELEQTNTIRHYGPIFPESTINALRFWKKLWQDEQRFVIHGLHRTLMDQPTYETSEFAEIVRNLRFSRPGNNYINELNITSPAMYGDKHTTGDIAPNDRFAMLVAFINSTDFLYTAIPEEKVGGNDTQTGPQTSSLTDLVPTRLHSFLNHNLSKLKILNRAQQTVKNILSNDCLNQLKHYVKHTGKNEILKILQE

Function

Essential for the correct assembly and maturation of the core of the virion.

Miscellaneous

Expressed late in the infection.

Similarity

Belongs to the asfivirus polyprotein pp62 family.

Keywords

Complete proteome Host cytoplasm Ubl conjugation Virion Direct protein sequencing Reference proteome

Feature

chain Polyprotein pp62

Uniprot

A0A0C5AZE8 A0A2Z5DGI1 E0WM44 X2L313 D4I5T1 A9JLY9 + More
A0A0A1E146 P0CA08 Q65179 A0A0A1DY09 P0CA07 A9JL58 P0CA05 A0A0C5AYY4 P0CA06 A0A2Z6S924

Pubmed

25645905 21470447 18198370 25406173 26618713 11831707 + More
7853518 8474154 9223468 12438573 19846532

EMBL

KM111295 AJL34272.1 MH025916 MH025917 MH025918 MH025919 + More
MH025920 AXB49322.1 AXB49496.1 AXB49668.1 AXB49839.1 AXB50012.1 FR682468 CBW46760.1 KJ380911 MG939583 MG939587 MG939588 LS478113 AHN94308.1 AXZ95868.1 AXZ96056.1 AXZ96151.1 SPS73517.1 FN557520 CBH29194.1 AM712240 CAN10443.1 KM262845 KM102979 KP055815 KX354450 AIY22443.1 AJZ77059.1 AKO62775.1 AOO54444.1 AY261366 U18466 S75219 M96354 KM262844 AIY22285.1 AY261363 AM712239 CAN10193.1 AY261360 KM111294 AJL34107.1 AY261361 BEXD01004040 GBC05960.1

Proteomes

UP000101566 UP000141072 UP000101090 UP000108903 UP000110401 UP000117635 + More
UP000241813 UP000266411 UP000000858 UP000000624 UP000142390 UP000000859 UP000130745 UP000000861 UP000105860 UP000000860 UP000247702

PRIDE

Q65179 P0CA07 P0CA05 P0CA06

ProteinModelPortal

A0A0C5AZE8 A0A2Z5DGI1 E0WM44 X2L313 D4I5T1 A9JLY9 + More
A0A0A1E146 P0CA08 Q65179 A0A0A1DY09 P0CA07 A9JL58 P0CA05 A0A0C5AYY4 P0CA06 A0A2Z6S924

PDB

5K5X E-value=5.49942, Score=69

Ontologies

KEGG

vg:22220323

GO

GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0019012 C:virion

Subcellular Location

From MSLVP

Host nucleus > host nucleolus. Host cytoplasm. Secreted.

From Uniprot

Found in perinuclear cytoplasmic viral factories during assembly. With evidence from 2 publications.
Virion Located in the core shell, which functions like a matrix between the DNA and the inner envelope. With evidence from 2 publications.

Topology

Length:

534

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01971

Exp number, first 60 AAs:

0.00437

Total prob of N-in:

0.00370

outside

1 - 534

Population Genetic Test Statistics

Pi

0.0304276

Theta

0.0386839

Tajima's D

-0.6386565

CLR

0.063536

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein Protein	QED21688.1 AKO62775.1
China_AnhuiXCGQ_2018	Protein Protein	QED21688.1 AYW34065.1
China_ASFV-SY18_2018	Protein	AKO62775.1
Poland_Pol16_20186_o7_2016-2017	Protein Protein	QED21688.1 AXZ95868.1
Poland_Pol16_20538_o9_2016-2017	Protein Protein	AJL34107.1 QED21688.1
Poland_Pol16_20540_o10_2016-2017	Protein Protein	AJL34107.1 QED21688.1
Poland_Pol16_29413_o23_2016-2017	Protein Protein	AJL34107.1 QED21688.1
Poland_Pol17_03029_C201_2016-2017	Protein Protein	QED21688.1 AXZ96056.1
Poland_Pol17_04461_C210_2016-2017	Protein Protein	QED21688.1 AXZ96151.1
Poland_Pol17_05838_C220_2016-2017	Protein Protein	AJL34107.1 QED21688.1
Poland_ASFV_POL_Podlaskie_2015	Protein Protein	AJL34107.1 QED21688.1
Uganda_R8_2015	Protein Protein	QED21688.1 AXB49322.1
Uganda_R7_2015	Protein Protein	QED21688.1 AXB49496.1
Uganda_R25_2015	Protein Protein	QED21688.1 AXB49668.1
Uganda_N10_2015	Protein Protein	QED21688.1 AXB49839.1
Uganda_R35_2015	Protein Protein	QED21688.1 AXB50012.1
Estonia_2014	Protein Protein	QED21688.1 SPS73517.1
Italy_26544_OG10_2010	Protein Protein	QED21688.1 AJZ77059.1
Italy_47_Ss_2008_2008	Protein Protein	QED21688.1 AOO54444.1
Portugal_OURT_88.3_1988	Protein Protein	QED21688.1 CAN10443.1
Spain_E75_1975	Protein Protein	QED21688.1 CBH29194.1
Russia_Georgia_2007	Protein Protein	QED21688.1 CBW46760.1
Kenya_Tk1_2005	Protein Protein Protein	AJL34107.1 QED21688.1 AJL34107.1
Kenya_Bus_2006	Protein Protein	QED21688.1 AJL34272.1
Russia_Odintsovo_2014	Protein Protein	AJL34107.1 QED21688.1
Portugal_L60_1960	Protein Protein	QED21688.1 AIY22285.1
Portugal_NHV_1968	Protein Protein	QED21688.1 AIY22443.1
Russia_Kashino_2013	Protein Protein	AJL34107.1 QED21688.1
Benin_1997	Protein Protein	QED21688.1 CAN10193.1
South_Africa_KNP_Pretorisuskop_1996	Protein Protein	AJL34107.1 QED21688.1
South_Africa_MGR_Mkuzi_1979	Protein Protein	AJL34107.1 QED21688.1
Malawi_Lil_1983	Protein Protein	AJL34107.1 QED21688.1
Kenya_1950	Protein Protein	AJL34107.1 QED21688.1
Namibia_Warthog_1980	Protein Protein	AJL34107.1 QED21688.1
South_Africa_Warmbaths_1987	Protein Protein	AJL34107.1 QED21688.1
Malawi_Tengani_1962	Protein Protein	AJL34107.1 QED21688.1
China_HLJ_2018	Protein Protein	QED21688.1 QBH90582.1
China_LN_2018	Protein Protein	QED21688.1 QBH90767.1
China_wbBS01_2018	Protein Protein	QED21688.1 QDL88125.1
Ukraine_Kyiv_2014	Protein Protein	QED21688.1 QED21689.1
Belgium_Etalle_wb_2018	Protein Protein	QED21688.1 QED90537.1
Lithuania_LT14_2014	Protein Protein	QED21688.1 QEY87900.1
South_Africa_1985	Protein Protein	QED21688.1 QGM12924.1
South_Africa_2_2008	Protein Protein	QED21688.1 QGM12850.1
Zambia_LIV_1983	Protein	QED21688.1

Sequence