Strain

Ukraine_Kyiv_2014 (Region: Ukraine; Strain: ASFV/Kyiv/2016/131; Date: 2014)

Gene

CP530R

Description

Annotated in NCBI, ASFV_Kyiv_2016_131_00152

Location

MN194591.1(+):127077-127388

GenBank Accession

QED21688.1

Full name

Polyprotein pp62 + More

Alternative Name

60 kDa polyprotein
62 kDa polyprotein
60 kDa polyprotein
62 kDa polyprotein

CDS

ATGCCCTCTAATATGAAACAGTTTTGCAAGATTTCTGTATGGCTACAGCAGCACGATCCAGATTTATTAGAAATTATCAACAACTTATGTATGCTTGGCAATTTATCCGCGGCAAAGTACAAACACGGAGTTACCTTCATTTACCCCAAACAGGCAAAGATCCGCGATGAAATAAAAAAACATGCCTACTCCAATGACCCTTCACAAGCCATAAAGACCTTAGAATCACTCATCCTTCCATTTTACATTCCCACTCCAGCGGAGTTCACCGGGGAAATCGGCTCCCTACACCGGAGTGAAATTAGAGGTTGA

Protein

MPSNMKQFCKISVWLQQHDPDLLEIINNLCMLGNLSAAKYKHGVTFIYPKQAKIRDEIKKHAYSNDPSQAIKTLESLILPFYIPTPAEFTGEIGSLHRSEIRGX

Function

Essential for the correct assembly and maturation of the core of the virion.

Miscellaneous

Expressed late in the infection.
Expressed late in the infection.

Similarity

Belongs to the asfivirus polyprotein pp62 family.

Keywords

Complete proteome Host cytoplasm Ubl conjugation Virion Direct protein sequencing Reference proteome

Feature

chain Polyprotein pp62

Uniprot

A0A5B8XBD3 A0A0A1DY09 D4I5T1 A9JLY9 A0A0A1E146 P0CA08 + More
Q65179 E0WM44 X2L313 P0CA06 P0CA05 P0CA07 A9JL58 A0A0C5AYY4 A0A0C5AZE8 A0A2Z5DGI1 A0A2Z6S924

Pubmed

25406173 18198370 26618713 11831707 7853518 8474154 + More
9223468 12438573 19846532 21470447 25645905

EMBL

MN194591 QED21688.1 KM262844 AIY22285.1 FN557520 CBH29194.1 + More
AM712240 CAN10443.1 KM262845 KM102979 KP055815 KX354450 AIY22443.1 AJZ77059.1 AKO62775.1 AOO54444.1 AY261366 U18466 S75219 M96354 FR682468 CBW46760.1 KJ380911 MG939583 MG939587 MG939588 LS478113 AHN94308.1 AXZ95868.1 AXZ96056.1 AXZ96151.1 SPS73517.1 AY261361 AY261360 AY261363 AM712239 CAN10193.1 KM111294 AJL34107.1 KM111295 AJL34272.1 MH025916 MH025917 MH025918 MH025919 MH025920 AXB49322.1 AXB49496.1 AXB49668.1 AXB49839.1 AXB50012.1 BEXD01004040 GBC05960.1

Proteomes

UP000321214 UP000142390 UP000101090 UP000108903 UP000110401 UP000117635 + More
UP000241813 UP000266411 UP000000858 UP000000624 UP000141072 UP000000860 UP000000861 UP000000859 UP000130745 UP000105860 UP000101566 UP000247702

PRIDE

Q65179 P0CA06 P0CA05 P0CA07

ProteinModelPortal

A0A5B8XBD3 A0A0A1DY09 D4I5T1 A9JLY9 A0A0A1E146 P0CA08 + More
Q65179 E0WM44 X2L313 P0CA06 P0CA05 P0CA07 A9JL58 A0A0C5AYY4 A0A0C5AZE8 A0A2Z5DGI1 A0A2Z6S924

PDB

5K5X E-value=0.344544, Score=71

Ontologies

KEGG

vg:22220323

GO

GO:0044220 C:host cell perinuclear region of cytoplasm
GO:0019012 C:virion

Subcellular Location

From MSLVP

Host nucleus. Host cytoplasm.

From Uniprot

   Found in perinuclear cytoplasmic viral factories during assembly.   With evidence from 2 publications.
Virion   Located in the core shell, which functions like a matrix between the DNA and the inner envelope.   With evidence from 2 publications.
   Found in perinuclear cytoplasmic viral factories during assembly.   With evidence from 2 publications.
Virion   Located in the core shell, which functions like a matrix between the DNA and the inner envelope.   With evidence from 2 publications.

Topology

Length:

104

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.02191

Exp number, first 60 AAs:

0.0125

Total prob of N-in:

0.39557

Length:

103

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.02216

Exp number, first 60 AAs:

0.01256

Total prob of N-in:

0.39730

outside

1 - 104

outside

1 - 103

Population Genetic Test Statistics

Pi

0.0272174

Theta

0.0295818

Tajima's D

-0.2619737

CLR

0

Interpretation

Possibly Balancing Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971
China_AnhuiXCGQ_2018
China_ASFV-SY18_2018
Poland_Pol16_20186_o7_2016-2017
Poland_Pol16_20538_o9_2016-2017
Poland_Pol16_20540_o10_2016-2017
Poland_Pol16_29413_o23_2016-2017
Poland_Pol17_03029_C201_2016-2017
Poland_Pol17_04461_C210_2016-2017
Poland_Pol17_05838_C220_2016-2017
Poland_ASFV_POL_Podlaskie_2015
Uganda_R8_2015
Uganda_R7_2015
Uganda_R25_2015
Uganda_N10_2015
Uganda_R35_2015
Estonia_2014
Italy_26544_OG10_2010
Italy_47_Ss_2008_2008
Portugal_OURT_88.3_1988
Spain_E75_1975
Russia_Georgia_2007
Kenya_Tk1_2005
Kenya_Bus_2006
Russia_Odintsovo_2014
Portugal_L60_1960
Portugal_NHV_1968
Russia_Kashino_2013
Benin_1997
South_Africa_KNP_Pretorisuskop_1996
South_Africa_MGR_Mkuzi_1979
Malawi_Lil_1983
Kenya_1950
Namibia_Warthog_1980
South_Africa_Warmbaths_1987
Malawi_Tengani_1962
China_HLJ_2018
China_LN_2018
China_wbBS01_2018
Ukraine_Kyiv_2014	Protein	QED21688.1
Belgium_Etalle_wb_2018
Lithuania_LT14_2014
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence