Strain

Portugal_L60_1960 (Region: Portugal; Strain: L60; Date: 1960)

Gene

thymidylate kinase

Description

Annotated in NCBI, gene expression: early

Location

KM262844.1(-):42151-42873

GenBank Accession

AIY22226.1

Full name

Thymidylate kinase + More
Probable thymidylate kinase

Alternative Name

dTMP kinase

CDS

ATGCGTGGAATACTTATTACCATCGAGGGAATCAATGGTGTGGGAAAAAGCACACAGGCAATGAGATTAAAAAAGGCATTAGAATGCATGGATTATAATGCTGTATGTATACGTTTTCCTAATCCAGACACGACCACCGGTGGCCTTATATTGCAGGTGTTAAATAAAATGACAGAAATGTCATCGGAACAATTGCATAAATTATTTACAAAACATCATAGTGAATTTAGCGCAGAAATTGCAGCACTACTTAAACTAAATTTTATTGTAATAGTGGACCATTATATTTGGTCTGGTTTAGCATATGCTCAAGCAGATGGAATCACGATTGAAACCAAAAATATCTTTAAACCGGATTATACATTCTTTTTATCTTCAAAGAAACCATTGAATGAAAAACCTTTAACCTTACAACGTTTATTTGAAACAAAAGAAAAACAAGAAACAATTTTTACTAATTTTACTATTATCATGAATGATGTACCTAAAAATAGGCTTTGTATTATTCCGGCAACTCTTAATAAGGAAATTATTCATACAATGATTTTAACAAAAACGATAAAAGTTTTTGACAACAACTCATGTTTAAATTATATTAAAATGTACGATGATAAGTATTTAAATGTCCAGGATCTGAATTTATTCGATTTCGATTGGCAAAAATGTATTGAAGATAATAATGATAAAGAAGAATACGACGATGATGATGGCTTTATTATTTAA

Protein

MRGILITIEGINGVGKSTQAMRLKKALECMDYNAVCIRFPNPDTTTGGLILQVLNKMTEMSSEQLHKLFTKHHSEFSAEIAALLKLNFIVIVDHYIWSGLAYAQADGITIETKNIFKPDYTFFLSSKKPLNEKPLTLQRLFETKEKQETIFTNFTIIMNDVPKNRLCIIPATLNKEIIHTMILTKTIKVFDNNSCLNYIKMYDDKYLNVQDLNLFDFDWQKCIEDNNDKEEYDDDDGFII

Catalytic Activity

ATP + dTMP = ADP + dTDP

Similarity

Belongs to the thymidylate kinase family.

Keywords

ATP-binding Complete proteome Kinase Nucleotide biosynthesis Nucleotide-binding Reference proteome Transferase Acetylation Alternative splicing

Feature

chain Thymidylate kinase
splice variant In isoform Short.

Uniprot

A0A0N6WHZ1 A9JKX1 D4I5M3 A9JLM6 A0A0A1E300 P42490 + More
P0C8G2 P0C8G1 E0WMH3 A0A2X0RUU2 A0A0C5AZC7 P0C8F9 A0A2Z5DGC8 D6WW08 A0A286UJZ7 A0A1Y3BL63 A0A3P3YJU7 A0A0H5QIM5 A0A3M7QN55 G5E3F1 A0A166JFD7 A0A2A3EH77 A0A0L8G043 A0A0L8HLB6 V8NMQ6 A0A2P0VN24 C4WTA6 A0A1V9XDP3 A0A163EG61 A0A3B1KAT6 A0A2Y9RQ05 B6K7E8 E4URU8 B0K028 A0A2K6G7K9 A0A0F7Z299 A0A267DTH8 A0A267HA90 A0A2D4FVS7 A0A1I8GGD6 A0A0E3Q2P1 A0A2B7Z168 A0A0F8NK98 P97930-2 Q6GRA7 H2TXQ6 A0A2H8TXE8 A0A1U7QYB1 J9K470 G1SQ07 A0A212EPA4 A0A2Y9RSG8 A0A1Y2C173 A1C4Z3 D3ZUJ5 G3QFV9 A0A177TS54 A0A177UW63 A0A0V0GB06 A0A1I8HW21 A0A1B8DWH0 A0A0F8S9T4 A0A0F8E1V8 A0A2K5D8G5 A0A1Q3ENU6 A0A2J8IPB7 A0A2J8RPT4 G5E9E9 A0A1S3K4Q8 A0A212CGJ5 A0A3B3U8P7 H0W009 V5GJC1 G3NKS7 G3NKU1 A0A088AK34 W9S4H8 R4FN90 A0A1D5PKC2 A0A091CWJ4 A0A1B6EFI8 A0A3P8TZ48 Q6DF36 A0A0P4VS64 T1HFS6 A1CZX2 A0A1A6HU02 Q6NUD2 A0A023F7Z7 A0A1L8G4G1 G7DU40 A0A2P6S678 A0A0F8DJY8 A0A0F8F6C2 A0A0F8C5U1 A0A0F8DQF2 A0A3B5AJ97 L1JWU7 A0A287BR54

EC Number

2.7.4.9

Pubmed

26618713 18198370 25406173 8393914 11831707 21470447 + More
25645905 18362917 19820115 30375419 26659563 24297900 29649682 28327890 25329095 21511999 22951933 15489334 15057822 26392545 25756680 8845311 16141072 21183079 10349636 11042159 11076861 11217851 12466851 12040188 16141073 21551351 21993624 22118469 18404212 15632090 29295979 11181995 15815621 19413330 21269460 25944712 15592404 20431018 27129103 25474469 27762356 21478649 23201678

EMBL

KP055815 AKO62717.1 AM712239 CAN10134.1 FN557520 CBH29136.1 + More
AM712240 CAN10383.1 KM262844 KM262845 KM102979 KX354450 AIY22226.1 AIY22385.1 AJZ77121.1 AOO54362.1 Z19544 U18466 AY261366 AY261363 FR682468 CBW46701.1 MG939583 MG939587 MG939588 LS478113 AXZ95812.1 AXZ96000.1 AXZ96095.1 SPS73458.1 KM111294 AJL34050.1 AY261360 MH025916 MH025917 MH025918 MH025919 MH025920 AXB49264.1 AXB49438.1 AXB49610.1 AXB49781.1 AXB49954.1 KQ971361 EFA08206.2 NBII01000004 PAV19943.1 MUJZ01016332 OTF80847.1 OVEO01000013 SPR00040.1 HACM01001055 CRZ01497.1 REGN01005694 RNA12425.1 JP287915 AEQ17250.1 KV424479 KZV74510.1 KZ288256 PBC30569.1 KQ424852 KOF70367.1 KQ417858 KOF90031.1 AZIM01002690 ETE63544.1 KY322437 AUF82317.1 AK340538 BAH71126.1 MNPL01014534 OQR71462.1 KV441026 KV440973 OAD67069.1 OAD78460.1 KE651168 EEB09452.1 DS989824 EFR01220.1 AC109427 BC159428 AAI59429.1 GBEX01003485 JAI11075.1 NIVC01003210 NIVC01001800 NIVC01000199 PAA52608.1 PAA63936.1 PAA88241.1 NIVC01000010 PAA94462.1 IACJ01093317 LAA51595.1 CP009520 AKB42442.1 PDNA01000009 PGH27150.1 JJQL01000002 KKH51032.1 S82852 S82853 AK088416 BC030178 BC069902 AK011955 AK019284 AK019445 AK160747 CH466520 AAH69902.1 BAB27937.1 BAB31646.1 BAB31722.1 BAE35986.1 EDL39931.1 GFXV01006666 MBW18471.1 ABLF02029968 AGBW02013517 OWR43287.1 MCGR01000140 ORY40627.1 DS027004 EAW14761.1 CH473997 EDL91900.1 CABD030019471 CABD030019472 LWDF01000026 OAJ06127.1 LWDG01000052 OAJ19869.1 GECL01000844 JAP05280.1 KV455024 OBT63461.1 JJQY01000074 KKH96443.1 JJOV01000078 KKG08918.1 BDGU01000813 GAW08865.1 NBAG03000666 PNI12345.1 NDHI03003662 PNJ10537.1 AC133528 CH471063 EAW71286.1 MKHE01000020 OWK05065.1 AAKN02019244 GALX01006754 JAB61712.1 KE346069 EXC25420.1 GAHY01001394 JAA76116.1 AADN04000198 KN124008 KFO22558.1 GEDC01000601 JAS36697.1 AAMC01060133 BC076907 CR761321 AAH76907.1 CAJ81735.1 GDKW01001174 JAI55421.1 ACPB03001438 ACPB03001439 DS027686 EAW24292.1 LZPO01017287 OBS81212.1 BC068663 AAH68663.1 GBBI01001330 JAC17382.1 CM004475 OCT78789.1 BABT02000028 GAA94100.1 PDCK01000040 PRQ54178.1 JJOX01000022 KKG17984.1 JJOZ01000050 KKG22598.1 JJOW01000336 KKG10331.1 JJOY01000048 KKG19934.1 JH992971 EKX53061.1 AEMK02000101

Proteomes

UP000241813 UP000130745 UP000101090 UP000108903 UP000110401 UP000117635 + More
UP000142390 UP000266411 UP000000624 UP000000858 UP000000859 UP000141072 UP000105860 UP000000861 UP000007266 UP000217199 UP000077086 UP000242457 UP000053454 UP000244773 UP000192247 UP000077315 UP000018467 UP000248480 UP000001744 UP000002669 UP000002494 UP000233160 UP000215902 UP000095280 UP000033096 UP000224634 UP000034377 UP000000589 UP000005226 UP000189706 UP000007819 UP000001811 UP000007151 UP000193467 UP000006701 UP000001519 UP000077521 UP000078113 UP000091879 UP000034028 UP000034052 UP000233020 UP000188533 UP000005640 UP000085678 UP000261500 UP000005447 UP000007635 UP000005203 UP000030645 UP000000539 UP000028990 UP000008143 UP000015103 UP000006702 UP000186698 UP000009131 UP000238479 UP000034220 UP000034651 UP000034397 UP000034930 UP000261400 UP000011087 UP000008227

PRIDE

P42490 P0C8G2 P0C8G1 P97930 G5E9E9

Pfam

PF02223 Thymidylate_kin
PF00183 HSP90

Interpro

IPR039430   Thymidylate_kin_like_dom
IPR027417   P-loop_NTPase
IPR018095   Thymidylate_kin_CS
IPR018094   Thymidylate_kinase
IPR037196   HSP90_C
IPR020575   Hsp90_N
IPR036890   HATPase_C_sf
IPR001404   Hsp90_fam
IPR003594   HATPase_C
IPR020568   Ribosomal_S5_D2-typ_fold

SUPFAM

SSF52540   SSF52540
SSF55874   SSF55874
SSF54211   SSF54211
SSF110942   SSF110942

Gene 3D

1.20.120.790 3.30.565.10

CDD

cd00075 HATPase_c

ProteinModelPortal

A0A0N6WHZ1 A9JKX1 D4I5M3 A9JLM6 A0A0A1E300 P42490 + More
P0C8G2 P0C8G1 E0WMH3 A0A2X0RUU2 A0A0C5AZC7 P0C8F9 A0A2Z5DGC8 D6WW08 A0A286UJZ7 A0A1Y3BL63 A0A3P3YJU7 A0A0H5QIM5 A0A3M7QN55 G5E3F1 A0A166JFD7 A0A2A3EH77 A0A0L8G043 A0A0L8HLB6 V8NMQ6 A0A2P0VN24 C4WTA6 A0A1V9XDP3 A0A163EG61 A0A3B1KAT6 A0A2Y9RQ05 B6K7E8 E4URU8 B0K028 A0A2K6G7K9 A0A0F7Z299 A0A267DTH8 A0A267HA90 A0A2D4FVS7 A0A1I8GGD6 A0A0E3Q2P1 A0A2B7Z168 A0A0F8NK98 P97930-2 Q6GRA7 H2TXQ6 A0A2H8TXE8 A0A1U7QYB1 J9K470 G1SQ07 A0A212EPA4 A0A2Y9RSG8 A0A1Y2C173 A1C4Z3 D3ZUJ5 G3QFV9 A0A177TS54 A0A177UW63 A0A0V0GB06 A0A1I8HW21 A0A1B8DWH0 A0A0F8S9T4 A0A0F8E1V8 A0A2K5D8G5 A0A1Q3ENU6 A0A2J8IPB7 A0A2J8RPT4 G5E9E9 A0A1S3K4Q8 A0A212CGJ5 A0A3B3U8P7 H0W009 V5GJC1 G3NKS7 G3NKU1 A0A088AK34 W9S4H8 R4FN90 A0A1D5PKC2 A0A091CWJ4 A0A1B6EFI8 A0A3P8TZ48 Q6DF36 A0A0P4VS64 T1HFS6 A1CZX2 A0A1A6HU02 Q6NUD2 A0A023F7Z7 A0A1L8G4G1 G7DU40 A0A2P6S678 A0A0F8DJY8 A0A0F8F6C2 A0A0F8C5U1 A0A0F8DQF2 A0A3B5AJ97 L1JWU7 A0A287BR54

PANTHER

PTHR11528

PDB

1E9F E-value=3.06859e-12, Score=171

Ontologies

KEGG

vg:22220417

GO

GO:0016301  F:kinase activity
GO:0004798  F:thymidylate kinase activity
GO:0005524  F:ATP binding
GO:0006235  P:dTTP biosynthetic process
GO:0006233  P:dTDP biosynthetic process
GO:0009041  F:uridylate kinase activity
GO:0004550  F:nucleoside diphosphate kinase activity
GO:0006227  P:dUDP biosynthetic process
GO:0005737  C:cytoplasm
GO:0005739  C:mitochondrion
GO:0005634  C:nucleus
GO:0005829  C:cytosol
GO:0045445  P:myoblast differentiation
GO:0005759  C:mitochondrial matrix
GO:0043627  P:response to estrogen
GO:0005758  C:mitochondrial intermembrane space
GO:0046686  P:response to cadmium ion
GO:0071363  P:cellular response to growth factor stimulus
GO:0050145  F:nucleoside monophosphate kinase activity
GO:0009165  P:nucleotide biosynthetic process
GO:0051082  F:unfolded protein binding
GO:0006457  P:protein folding

Subcellular Location

From MSLVP

Single-Pass Membrane

Topology

Length:

240

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.16269

Exp number, first 60 AAs:

0.00197

Total prob of N-in:

0.03101

outside

1 - 240

Population Genetic Test Statistics

Pi

0.0829388

Theta

0.0944343

Tajima's D

-0.5114191

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62717.1
China_AnhuiXCGQ_2018	Protein	AYW34007.1
China_ASFV-SY18_2018	Protein	AKO62717.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95812.1
Poland_Pol16_20538_o9_2016-2017	Genetic Remain	CAN10134.1
Poland_Pol16_20540_o10_2016-2017	Genetic Remain	CAN10134.1
Poland_Pol16_29413_o23_2016-2017	Genetic Remain	CAN10134.1
Poland_Pol17_03029_C201_2016-2017	Protein	AXZ96000.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96095.1
Poland_Pol17_05838_C220_2016-2017	Genetic Remain	CAN10134.1
Poland_ASFV_POL_Podlaskie_2015	Genetic Remain	CAN10134.1
Uganda_R8_2015
Uganda_R7_2015
Uganda_R25_2015
Uganda_N10_2015
Uganda_R35_2015
Estonia_2014	Protein	SPS73458.1
Italy_26544_OG10_2010	Protein	AJZ77121.1
Italy_47_Ss_2008_2008	Protein	AOO54362.1
Portugal_OURT_88.3_1988	Protein	CAN10383.1
Spain_E75_1975	Protein	CBH29136.1
Russia_Georgia_2007	Protein	CBW46701.1
Kenya_Tk1_2005
Kenya_Bus_2006	Protein	AJL34215.1
Russia_Odintsovo_2014	Genetic Remain	CAN10134.1
Portugal_L60_1960	Protein	AIY22226.1
Portugal_NHV_1968	Protein	AIY22385.1
Russia_Kashino_2013	Genetic Remain	CAN10134.1
Benin_1997	Protein Protein	CAN10134.1 CAN10134.1
South_Africa_KNP_Pretorisuskop_1996	Genetic Remain	CAN10134.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10134.1
Malawi_Lil_1983	Protein	CAN10134.1
Kenya_1950
Namibia_Warthog_1980	Genetic Remain	CAN10134.1
South_Africa_Warmbaths_1987	Protein	CAN10134.1
Malawi_Tengani_1962	Protein	CAN10134.1
China_HLJ_2018	Protein	QBH90523.1
China_LN_2018	Protein	QBH90708.1
China_wbBS01_2018	Protein	QDL88066.1
Ukraine_Kyiv_2014	Protein	QED21592.1
Belgium_Etalle_wb_2018	Protein	QED90478.1
Lithuania_LT14_2014	Protein	QEY87841.1
South_Africa_1985	Genetic Remain	CAN10134.1
South_Africa_2_2008	Protein	CAN10134.1
Zambia_LIV_1983	Genetic Remain	CAN10134.1

Sequence