Strain

South_Africa_2_2008 (Region: South Africa;  Strain: RSA_2_2008;  Date: 2008)

Gene

pK196R

Description

Annotated in NCBI,  K196R

Location

MN336500.1(+):63256-63846

GenBank Accession

QGM12831.1

Full name

Thymidine kinase       + More
Thymidine kinase 1      

Alternative Name

Calmodulin-binding protein ThyB

CDS

ATGAATATATTCAGGAAGCTTATACCTGGAACAATTACCCTTGTGCTGGGACCCATGTTTGCCGGCAAAACTACGTTTCTTATTCATTGCATTTACATGCTCGAACGTTTGGAAAAAAAAGTACTCTTCATACAATCTACAAAAAACACCCGAGACAAAACTATTACAACACACTCCGGTATACAGCTACGATCCAAGCAATGTACAATCATACAAAGCACACAGTTATCTGTCGTGGGATCTCTCACCGATATCCATGCAGTTGTCATACATGCAGCGCATTTTTTTGTCGATTTATTACAATGCCGCACCTGGGCAGATGCAGAAAAAATTATTATTCTTGCGGGACTCAATGCTTCCTTCGAGCAGAAAATGTTTCCGCCCATCGTTCGTATTTTTCCTTACTGCAGCTGGGTTATGTATATTGGCCGCACCTGTATGCAATGTATCCGACATATTGCATGCTTTATTGTGCGTACGAACGCAGACAAGACGCTTATCCTTGCGGGAGGAAGTGTACTGTACGTATCATGTTGTATCAACTGTCTACAAAATACATTTATTATGCAGTTGCAACCTATTATATATTAA

Protein

MNIFRKLIPGTITLVLGPMFAGKTTFLIHCIYMLERLEKKVLFIQSTKNTRDKTITTHSGIQLRSKQCTIIQSTQLSVVGSLTDIHAVVIHAAHFFVDLLQCRTWADAEKIIILAGLNASFEQKMFPPIVRIFPYCSWVMYIGRTCMQCIRHIACFIVRTNADKTLILAGGSVLYVSCCINCLQNTFIMQLQPIIY

Function

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine.
Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine (By similarity).

Catalytic Activity

ATP + thymidine = ADP + dTMP + H(+)

Biophysicochemical Properties

5.1 uM for thymidine

Subunit

Interacts with calmodulin in the presence of Ca(2+).

Similarity

Belongs to the thymidine kinase family.

Keywords

ATP-binding   Complete proteome   DNA synthesis   Kinase   Metal-binding   Nucleotide-binding   Transferase   Virulence   Zinc   Reference proteome   Calmodulin-binding  

Feature

chain  Thymidine kinase

Uniprot

Q336E5   I6LA85   I6LA84   P0C8I4   P0C8I5   Q336E4   + More
A9JKZ6   D4I5N8   A9JLQ8   Q336F0   P18555   E0WMI8   Q336E6   Q336E3   Q336E2   P0C8I6   A0A1W5LD83   A0A0C5AWN5   Q336D8   P0C8I3   A0A0C5AWA4   K8F5H9   A0A0K8SGE3   A0A1Z5KTU8   Q27564   A0A0A9YI46   A0A146LTU8   A0A353J6W1   F0ZSA2  

EC Number

2.7.1.21

Pubmed

9811782    18198370    25406173    26618713    2389555    1987368    + More
11831707    21470447    29143187    25645905    28528879    15883042    17448496    8643615    15875012    11923193    12912885    25401762    26823975    30148503    21356102   

EMBL

AF504898    AAQ07942.1    AF504894    AAQ07938.1    AF504903    AAQ07929.1    + More
AY261361    AY261363    AF504899    AAQ07943.1    AM712239    CAN10149.1    FN557520    CBH29151.1    AM712240    CAN10398.1    AF504890    AF504891    AF504892    AF504893    AF504895    AF504896    AF504902    KM262844    KM262845    KM102979    KP055815    KX354450    AAQ07934.1    AAQ07935.1    AAQ07936.1    AAQ07937.1    AAQ07939.1    AAQ07940.1    AAQ07946.1    AIY22241.1    AIY22400.1    AJZ77133.1    AKO62732.1    AOO54381.1    Z21490    M63119    U18466    FR682468    CBW46716.1    AF504897    MG939583    MG939587    MG939588    LS478113    AAQ07941.1    AXZ95826.1    AXZ96014.1    AXZ96109.1    SPS73473.1    AF504900    AAQ07944.1    AF504901    AAQ07945.1    AY261366    KU199302    ANC68039.1    KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    AJL34229.1    AXB49279.1    AXB49453.1    AXB49625.1    AXB49796.1    AXB49969.1    AF504904    AF504906    AF504907    AAQ07930.1    AAQ07932.1    AAQ07933.1    AY261360    KM111294    AJL34064.1    FO082264    CCO20075.1    GBRD01013464    GBRD01013463    GBRD01013461    JAG52362.1    GFJQ02008444    JAV98525.1    AY192984    AF510846    U61990    AAFI02000131    AAB03673.1    AAM44288.1    AAO64434.1    EAL62823.1    GBHO01030448    GBHO01012303    GBHO01010378    GBHO01009703    GBHO01009695    JAG13156.1    JAG31301.1    JAG33226.1    JAG33901.1    JAG33909.1    GDHC01008084    JAQ10545.1    DNQG01000027    HBF67246.1    GL871154    EGC33181.1   

Proteomes

UP000000860    UP000000859    UP000130745    UP000101090    UP000108903    UP000110401    + More
UP000117635    UP000142390    UP000241813    UP000266411    UP000000624    UP000141072    UP000000858    UP000101566    UP000000861    UP000105860    UP000198341    UP000002195    UP000262368    UP000001064   

PRIDE

P0C8I4    P0C8I5    P18555   

Pfam

PF00265   TK

Interpro

IPR001267   Thymidine_kinase
IPR027417   P-loop_NTPase
IPR020633   Thymidine_kinase_CS

SUPFAM

SSF52540   SSF52540

ProteinModelPortal

Q336E5   I6LA85   I6LA84   P0C8I4   P0C8I5   Q336E4   + More
A9JKZ6   D4I5N8   A9JLQ8   Q336F0   P18555   E0WMI8   Q336E6   Q336E3   Q336E2   P0C8I6   A0A1W5LD83   A0A0C5AWN5   Q336D8   P0C8I3   A0A0C5AWA4   K8F5H9   A0A0K8SGE3   A0A1Z5KTU8   Q27564   A0A0A9YI46   A0A146LTU8   A0A353J6W1   F0ZSA2  

PANTHER

PTHR11441  

PDB

4UXI     E-value=3.10926e-22,     Score=256