Strain

Italy_26544_OG10_2010 (Region: Sardinian,Italy; Strain: 26544/OG10; Date: 2010)

Gene

Thymidine kinase

Description

Annotated in NCBI, non-essential

Location

KM102979.1(+):58428-59018

GenBank Accession

AJZ77133.1

Full name

Thymidine kinase

CDS

ATGAATATAATTAGGAAGCTTAAGCCTGGAACAATTAGCCTTGTGCTGGGACCCATGTTTGCCGGCAAAACTACGTTTCTTATTCATTGCATTTACATGCTCGAACGTTTGGAAAAAAAAGTAGTCTTCATAAAATCTACCAAAAACACCCGAGACAAAACTATTAAAACACACTCCGGTATACAGCTACGACCCAAGCAATGTAAAATCATAGAAAGCACACAGTTATCTGACGTGGGATCTCTCACCGATATCCATGCAGTTGTCGTAGATGAAGCGCATTTTTTTGACGATTTAATAAAATGCCGCACCTGGGCAGAGGAAGAAAAAATTATTATTCTTGCGGGACTCAATGCTTCCTTCGAGCAGAAAATGTTTCCGCCCATCGTTCGTATTTTTCCTTACTGCAGCTGGGTTAAGTATATTGGCCGCACCTGTATGAAATGTAACCAACATAATGCATGCTTTAATGTGCGTAAGAACGCAGACAAGACGCTTATCCTTGCGGGAGGAAGTGAACTGTACGTAACATGTTGTAACAACTGTCTAAAAAATACATTTATTAAGCAGTTGCAACCTATTAAATATTAA

Protein

MNIIRKLKPGTISLVLGPMFAGKTTFLIHCIYMLERLEKKVVFIKSTKNTRDKTIKTHSGIQLRPKQCKIIESTQLSDVGSLTDIHAVVVDEAHFFDDLIKCRTWAEEEKIIILAGLNASFEQKMFPPIVRIFPYCSWVKYIGRTCMKCNQHNACFNVRKNADKTLILAGGSELYVTCCNNCLKNTFIKQLQPIKY

Function

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine (By similarity).
Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine.

Catalytic Activity

ATP + thymidine = ADP + dTMP + H(+)

Similarity

Belongs to the thymidine kinase family.

Keywords

ATP-binding Complete proteome DNA synthesis Kinase Metal-binding Nucleotide-binding Transferase Virulence Zinc Reference proteome

Feature

chain Thymidine kinase

Uniprot

P0C8I5 E0WMI8 Q336E6 A9JKZ6 D4I5N8 A9JLQ8 + More
Q336F0 P18555 Q336E4 Q336E3 Q336E5 I6LA85 Q336E2 P0C8I6 I6LA84 P0C8I4 A0A1W5LD83 A0A0C5AWN5 Q336D8 P0C8I3 A0A0C5AWA4 A0A1D1ZNX1

EC Number

2.7.1.21

Pubmed

21470447 18198370 25406173 26618713 2389555 1987368 + More
11831707 9811782 29143187 25645905

EMBL

AY261363 FR682468 CBW46716.1 AF504897 MG939583 MG939587 + More
MG939588 LS478113 AAQ07941.1 AXZ95826.1 AXZ96014.1 AXZ96109.1 SPS73473.1 AM712239 CAN10149.1 FN557520 CBH29151.1 AM712240 CAN10398.1 AF504890 AF504891 AF504892 AF504893 AF504895 AF504896 AF504902 KM262844 KM262845 KM102979 KP055815 KX354450 AAQ07934.1 AAQ07935.1 AAQ07936.1 AAQ07937.1 AAQ07939.1 AAQ07940.1 AAQ07946.1 AIY22241.1 AIY22400.1 AJZ77133.1 AKO62732.1 AOO54381.1 Z21490 M63119 U18466 AF504899 AAQ07943.1 AF504900 AAQ07944.1 AF504898 AAQ07942.1 AF504894 AAQ07938.1 AF504901 AAQ07945.1 AY261366 AF504903 AAQ07929.1 AY261361 KU199302 ANC68039.1 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34229.1 AXB49279.1 AXB49453.1 AXB49625.1 AXB49796.1 AXB49969.1 AF504904 AF504906 AF504907 AAQ07930.1 AAQ07932.1 AAQ07933.1 AY261360 KM111294 AJL34064.1 GDKF01010125 JAT68497.1

Proteomes

UP000000859 UP000141072 UP000130745 UP000101090 UP000108903 UP000110401 + More
UP000117635 UP000142390 UP000241813 UP000266411 UP000000624 UP000000858 UP000000860 UP000101566 UP000000861 UP000105860

PRIDE

P0C8I5 P18555 P0C8I4

Pfam

PF00265 TK

Interpro

IPR001267   Thymidine_kinase
IPR020633   Thymidine_kinase_CS
IPR027417   P-loop_NTPase

SUPFAM

SSF52540 SSF52540

ProteinModelPortal

P0C8I5 E0WMI8 Q336E6 A9JKZ6 D4I5N8 A9JLQ8 + More
Q336F0 P18555 Q336E4 Q336E3 Q336E5 I6LA85 Q336E2 P0C8I6 I6LA84 P0C8I4 A0A1W5LD83 A0A0C5AWN5 Q336D8 P0C8I3 A0A0C5AWA4 A0A1D1ZNX1

PANTHER

PTHR11441

PDB

4UXI E-value=3.10926e-22, Score=256

Ontologies

KEGG

vg:22220432

GO

GO:0004797  F:thymidine kinase activity
GO:0046872  F:metal ion binding
GO:0005524  F:ATP binding
GO:0071897  P:DNA biosynthetic process
GO:0009405  P:pathogenesis
GO:0016021  C:integral component of membrane

Subcellular Location

From MSLVP

Single-Pass Membrane

Topology

Length:

196

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

19.04813

Exp number, first 60 AAs:

18.94958

Total prob of N-in:

0.76308

POSSIBLE N-term signal

sequence

inside

1 - 11

TMhelix

12 - 34

outside

35 - 196

Population Genetic Test Statistics

Pi

0.0329783

Theta

0.0409595

Tajima's D

-0.679924

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62732.1
China_AnhuiXCGQ_2018	Protein	AYW34022.1
China_ASFV-SY18_2018	Protein	AKO62732.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95826.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10149.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10149.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10149.1
Poland_Pol17_03029_C201_2016-2017	Protein	AXZ96014.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96109.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10149.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10149.1
Uganda_R8_2015
Uganda_R7_2015
Uganda_R25_2015
Uganda_N10_2015
Uganda_R35_2015
Estonia_2014	Protein	SPS73473.1
Italy_26544_OG10_2010	Protein	AJZ77133.1
Italy_47_Ss_2008_2008	Protein	AOO54381.1
Portugal_OURT_88.3_1988	Protein	CAN10398.1
Spain_E75_1975	Protein	CBH29151.1
Russia_Georgia_2007	Protein	CBW46716.1
Kenya_Tk1_2005
Kenya_Bus_2006
Russia_Odintsovo_2014	Protein	CAN10149.1
Portugal_L60_1960	Protein	AIY22241.1
Portugal_NHV_1968	Protein	AIY22400.1
Russia_Kashino_2013	Protein	CAN10149.1
Benin_1997	Protein Protein	CAN10149.1 CAN10149.1
South_Africa_KNP_Pretorisuskop_1996
South_Africa_MGR_Mkuzi_1979	Protein	CAN10149.1
Malawi_Lil_1983	Protein	CAN10149.1
Kenya_1950
Namibia_Warthog_1980	Protein	CAN10149.1
South_Africa_Warmbaths_1987	Protein	CAN10149.1
Malawi_Tengani_1962	Protein	CAN10149.1
China_HLJ_2018	Protein	QBH90538.1
China_LN_2018	Protein	QBH90723.1
China_wbBS01_2018	Protein	QDL88081.1
Ukraine_Kyiv_2014	Protein Protein	QED21609.1 QED21610.1
Belgium_Etalle_wb_2018	Protein	QED90493.1
Lithuania_LT14_2014	Protein	QEY87856.1
South_Africa_1985	Protein	QGM12905.1
South_Africa_2_2008	Protein	QGM12831.1
Zambia_LIV_1983	Protein	QGM12712.1

Sequence