Strain

Uganda_N10_2015 (Region: Uganda, Tororo district; Strain: N10; Date: 2015)

Gene

pA240L

Description

Annotated in NCBI, gene expression: early

Location

MH025919.1(-):46273-46980

GenBank Accession

AXB49781.1

Full name

Thymidylate kinase       + More
Putative thymidylate kinase 251L
dTMP kinase

Alternative Name

dTMP kinase

CDS

ATGCGTGGAGTACTTATTGCCATCGAAGGAATCAATGGTGTGGGGAAAAGCACACAAGCAAGAAAGCTAAAAAATGCATTGGAAAATAAGAGATATGATGTGATATATATGCATTTTCCCAGTCCAAACACGGACACCGGTAAGCTTATATTGGATGTATTAAATAAAATCGTAAAAATGCCATCAGAAGAATTGCACGGATTATTCACAAAACATCGATGTGAATTTACGGCAGAAATTGCAGCACTACTTAAACTAAATTTTATTGTAATTGTGGACCGTTATATTTGGTCTGGTTTGGCATATGCTCAAGCAGACAGAATAATGATTGATACCAAAAATACTTTCAATCCGGATTACACATTCTTTTTATCTTCAAACAAACCATTAAATGAAAAACCCTTACATTTACAACGATTGTATGAAACAGAAGAAAAACAAGAAATAATTTTTACTCAATTTATTAATATCATAAATGAAGTACCTAAAAATAAGTTTTGTATTATTCCTGCAAATCTTAATAAGGAAATAATTGATAAGATCATTTTCTCAAAAACGATAAAAGTTTTTGAGAAAAACTTAAATTTAGACTATATTAAAATGTATGATGGTATGTATTTTAATATTCACGACTTAGATTTAATCCGTTTTGATTGGCAAAAATGTATTGAAGAAGATGATGGCATAGATGAGGAACACGGATTATAA

Protein

MRGVLIAIEGINGVGKSTQARKLKNALENKRYDVIYMHFPSPNTDTGKLILDVLNKIVKMPSEELHGLFTKHRCEFTAEIAALLKLNFIVIVDRYIWSGLAYAQADRIMIDTKNTFNPDYTFFLSSNKPLNEKPLHLQRLYETEEKQEIIFTQFINIINEVPKNKFCIIPANLNKEIIDKIIFSKTIKVFEKNLNLDYIKMYDGMYFNIHDLDLIRFDWQKCIEEDDGIDEEHGL

Function

Catalyzes the conversion of dTMP to dTDP.

Catalytic Activity

ATP + dTMP = ADP + dTDP

Similarity

Belongs to the thymidylate kinase family.

Keywords

ATP-binding Complete proteome Kinase Nucleotide biosynthesis Nucleotide-binding Transferase Reference proteome

Feature

chain Thymidylate kinase

Uniprot

A0A2Z5DGC8 A0A0C5AZC7 P0C8F9 E0WMH3 A0A2X0RUU2 P0C8G2 + More
A0A0N6WHZ1 A9JKX1 D4I5M3 A9JLM6 A0A0A1E300 P42490 P0C8G1 A0A220T6K7 A0A0L8HLB6 A0A023F7Z7 A0A0V0GB06 C4WTA6 A0A2A3EH77 R4FN90 A0A0P4VS64 T1HFS6 A0A1B7P8T1 A0A069DQQ3 A0A224XKB1 D0AB90 A0A2A4JCZ2 A0A212EPA4 L7X3W4 A0A1B6EFI8 J9K470 F0UPZ4 C6HED4 A0A1J9PA31 A0A067REX9 G5E3F1 A6QT32 C0NTM9 A0A1B6LJ93 A0A163EG61 A0A0L8G043 A0A3M6YZH8 A0A2H8TXE8 A0A2H1VL46 W8C5G7 B6K7E8 A0A0N0BJJ9 A0A1Y1JRQ2 A0A017SNY0 A0A088AK34 A0A1E3BIW0 Q91FS1 A0A0L0G1C5 A0A0L0G0L3 D6WW08 A0A2C5ZA62 A0A212CGJ5 Q6DF36 F2RNV5 A0A1B6K446 A0A0J7KEL4 A0A1B6LBU9 V8NMQ6 A0A194QSK5 V5GJC1 A0A1J1IJ42 A0A3M7HM84 A0A1B6M0Z6 A0A1L8G4G1 A0A0N1PFY9 Q6NUD2 A0A3M6ZXQ0 A0A317W3D2 A0A3M7GXU5 M2LHW9 A0A336L6X4 A0A2P2ENS6 A0A059JA89 F2PS14 A0A336K3L0 A0A2Y9RQ05 A1CZX2 A0A1I8Q3P3 A0A0H1BM71 A0A319DFP8 A0A2Y9SP80 A0A0U5GR06 A0A2J8IPB7 A0A2J8RPT4 G5E9E9 A0A0H5QIM5 A0A1Y3BL63 A0A3P3YJU7 A0A3M7DCF2 G3QFV9 A0A2R5GGP8 A1C4Z3 A0A3M6WU44 A0A1F5WCZ8

EC Number

2.7.4.9

Pubmed

25645905 21470447 26618713 18198370 25406173 8393914 + More
11831707 28685222 25474469 27129103 26334808 22118469 23674305 24845553 19717792 29764372 24495485 21511999 24811710 27267057 11448171 17239238 18362917 19820115 20431018 22951933 24297900 26354079 27762356 23236275 18404212 11181995 15815621 19413330 21269460 25944712 27774985

EMBL

MH025916 MH025917 MH025918 MH025919 MH025920 AXB49264.1 + More
AXB49438.1 AXB49610.1 AXB49781.1 AXB49954.1 KM111294 AJL34050.1 AY261360 FR682468 CBW46701.1 MG939583 MG939587 MG939588 LS478113 AXZ95812.1 AXZ96000.1 AXZ96095.1 SPS73458.1 AY261366 KP055815 AKO62717.1 AM712239 CAN10134.1 FN557520 CBH29136.1 AM712240 CAN10383.1 KM262844 KM262845 KM102979 KX354450 AIY22226.1 AIY22385.1 AJZ77121.1 AOO54362.1 Z19544 U18466 AY261363 KY747497 ASK51348.1 KQ417858 KOF90031.1 GBBI01001330 JAC17382.1 GECL01000844 JAP05280.1 AK340538 BAH71126.1 KZ288256 PBC30569.1 GAHY01001394 JAA76116.1 GDKW01001174 JAI55421.1 ACPB03001438 ACPB03001439 LGUA01000010 OAX85408.1 GBGD01002927 JAC85962.1 GFTR01003511 JAW12915.1 FP102340 CBH09285.1 NWSH01001832 PCG69967.1 AGBW02013517 OWR43287.1 KC469893 AGC92695.1 GEDC01000601 JAS36697.1 ABLF02029968 DS990640 EGC47044.1 GG692424 EER41155.1 LGRN01000307 OJD13368.1 KK852730 KDR17511.1 JP287915 AEQ17250.1 CH476655 EDN02674.1 GG663371 EEH05390.1 GEBQ01024689 GEBQ01016298 JAT15288.1 JAT23679.1 KV441026 KV440973 OAD67069.1 OAD78460.1 KQ424852 KOF70367.1 QWIJ01000749 QWIK01000314 RMX79079.1 RMY08443.1 GFXV01006666 MBW18471.1 ODYU01003152 SOQ41543.1 GAMC01004884 JAC01672.1 KE651168 EEB09452.1 KQ435715 KOX79122.1 BDQF01000015 GAW83877.1 KK088413 EYE98501.1 JXNT01000003 ODM20884.1 AF303741 KQ241912 KNC82591.1 KNC82590.1 KQ971361 EFA08206.2 NJEU01000301 PHH76763.1 MKHE01000020 OWK05065.1 AAMC01060133 BC076907 CR761321 AAH76907.1 CAJ81735.1 GG698478 EGD93011.1 GECU01003532 GECU01001488 JAT04175.1 JAT06219.1 LBMM01008713 KMQ88679.1 GEBQ01018826 GEBQ01010692 JAT21151.1 JAT29285.1 AZIM01002690 ETE63544.1 KQ461155 KPJ08299.1 GALX01006754 JAB61712.1 CVRI01000054 CRL00243.1 QWIP01000777 QWIO01000007 RMY54745.1 RMZ14481.1 GEBQ01018403 GEBQ01010417 JAT21574.1 JAT29560.1 CM004475 OCT78789.1 KQ459232 KQ459127 KPJ02634.1 KPJ03781.1 BC068663 AAH68663.1 QWIL01000365 QWIM01000375 RMY19829.1 RMY35426.1 MSFL01000015 PWY79628.1 QWIQ01000120 RMZ05829.1 KB445559 EMC93777.1 UFQS01001813 UFQT01001813 SSX12427.1 SSX31878.1 BFBS01016141 GBF62709.1 AOKY01000242 KDB24755.1 DS995734 EGE04682.1 UFQS01000086 UFQT01000086 SSW99077.1 SSX19459.1 DS027686 EAW24292.1 LDEV01001147 KLJ12142.1 KZ825842 PYH96139.1 CDMC01000002 CEN60262.1 NBAG03000666 PNI12345.1 NDHI03003662 PNJ10537.1 AC133528 CH471063 EAW71286.1 HACM01001055 CRZ01497.1 MUJZ01016332 OTF80847.1 OVEO01000013 SPR00040.1 QWIN01000022 RMY61975.1 CABD030019471 CABD030019472 BEYU01000065 GBG29775.1 DS027004 EAW14761.1 QWIJ01000460 RMX82097.1 MFHJ01000037 OGF73493.1

Proteomes

UP000105860 UP000000861 UP000141072 UP000000858 UP000241813 UP000130745 + More
UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 UP000266411 UP000000624 UP000000859 UP000217428 UP000053454 UP000242457 UP000015103 UP000091918 UP000218220 UP000007151 UP000007819 UP000008142 UP000002624 UP000182235 UP000027135 UP000009297 UP000001631 UP000077315 UP000001744 UP000053105 UP000019804 UP000005203 UP000094569 UP000001359 UP000054560 UP000007266 UP000224854 UP000008143 UP000009172 UP000053240 UP000183832 UP000186698 UP000053268 UP000247233 UP000011761 UP000245106 UP000024533 UP000009169 UP000248480 UP000006702 UP000095300 UP000247810 UP000248484 UP000054771 UP000005640 UP000001519 UP000006701

PRIDE

P0C8G2 P42490 P0C8G1 G5E9E9

Pfam

PF02223   Thymidylate_kin
PF00370   FGGY_N
PF02782   FGGY_C

Interpro

IPR039430   Thymidylate_kin_like_dom
IPR027417   P-loop_NTPase
IPR018095   Thymidylate_kin_CS
IPR018094   Thymidylate_kinase
IPR017986   WD40_repeat_dom
IPR040251   SEC31-like
IPR001680   WD40_repeat
IPR036322   WD40_repeat_dom_sf
IPR018485   Carb_kinase_FGGY_C
IPR015943   WD40/YVTN_repeat-like_dom_sf
IPR018484   Carb_kinase_FGGY_N

SUPFAM

SSF52540 SSF52540
SSF50978 SSF50978

Gene 3D

2.130.10.10

ProteinModelPortal

A0A2Z5DGC8 A0A0C5AZC7 P0C8F9 E0WMH3 A0A2X0RUU2 P0C8G2 + More
A0A0N6WHZ1 A9JKX1 D4I5M3 A9JLM6 A0A0A1E300 P42490 P0C8G1 A0A220T6K7 A0A0L8HLB6 A0A023F7Z7 A0A0V0GB06 C4WTA6 A0A2A3EH77 R4FN90 A0A0P4VS64 T1HFS6 A0A1B7P8T1 A0A069DQQ3 A0A224XKB1 D0AB90 A0A2A4JCZ2 A0A212EPA4 L7X3W4 A0A1B6EFI8 J9K470 F0UPZ4 C6HED4 A0A1J9PA31 A0A067REX9 G5E3F1 A6QT32 C0NTM9 A0A1B6LJ93 A0A163EG61 A0A0L8G043 A0A3M6YZH8 A0A2H8TXE8 A0A2H1VL46 W8C5G7 B6K7E8 A0A0N0BJJ9 A0A1Y1JRQ2 A0A017SNY0 A0A088AK34 A0A1E3BIW0 Q91FS1 A0A0L0G1C5 A0A0L0G0L3 D6WW08 A0A2C5ZA62 A0A212CGJ5 Q6DF36 F2RNV5 A0A1B6K446 A0A0J7KEL4 A0A1B6LBU9 V8NMQ6 A0A194QSK5 V5GJC1 A0A1J1IJ42 A0A3M7HM84 A0A1B6M0Z6 A0A1L8G4G1 A0A0N1PFY9 Q6NUD2 A0A3M6ZXQ0 A0A317W3D2 A0A3M7GXU5 M2LHW9 A0A336L6X4 A0A2P2ENS6 A0A059JA89 F2PS14 A0A336K3L0 A0A2Y9RQ05 A1CZX2 A0A1I8Q3P3 A0A0H1BM71 A0A319DFP8 A0A2Y9SP80 A0A0U5GR06 A0A2J8IPB7 A0A2J8RPT4 G5E9E9 A0A0H5QIM5 A0A1Y3BL63 A0A3P3YJU7 A0A3M7DCF2 G3QFV9 A0A2R5GGP8 A1C4Z3 A0A3M6WU44 A0A1F5WCZ8

PANTHER

PTHR13923

PDB

2WWI E-value=1.24041e-12, Score=174

Ontologies

KEGG

vg:22220417 vg:1733213

GO

GO:0006233  P:dTDP biosynthetic process
GO:0004798  F:thymidylate kinase activity
GO:0005524  F:ATP binding
GO:0006235  P:dTTP biosynthetic process
GO:0016301  F:kinase activity
GO:0009041  F:uridylate kinase activity
GO:0004550  F:nucleoside diphosphate kinase activity
GO:0006227  P:dUDP biosynthetic process
GO:0005737  C:cytoplasm
GO:0005739  C:mitochondrion
GO:0005634  C:nucleus
GO:0005829  C:cytosol
GO:0006888  P:endoplasmic reticulum to Golgi vesicle-mediated transport
GO:0016773  F:phosphotransferase activity, alcohol group as acceptor
GO:0005975  P:carbohydrate metabolic process
GO:0071363  P:cellular response to growth factor stimulus

Subcellular Location

From MSLVP

Single-Pass Membrane

Topology

Length:

235

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.19874

Exp number, first 60 AAs:

0.00017

Total prob of N-in:

0.02718

outside

1 - 235

Population Genetic Test Statistics

Pi

0.081592

Theta

0.0932965

Tajima's D

-0.5283898

CLR

0.508615

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971
China_AnhuiXCGQ_2018
China_ASFV-SY18_2018
Poland_Pol16_20186_o7_2016-2017
Poland_Pol16_20538_o9_2016-2017
Poland_Pol16_20540_o10_2016-2017
Poland_Pol16_29413_o23_2016-2017
Poland_Pol17_03029_C201_2016-2017
Poland_Pol17_04461_C210_2016-2017
Poland_Pol17_05838_C220_2016-2017
Poland_ASFV_POL_Podlaskie_2015
Uganda_R8_2015	Protein	AXB49264.1
Uganda_R7_2015	Protein	AXB49438.1
Uganda_R25_2015	Protein	AXB49610.1
Uganda_N10_2015	Protein	AXB49781.1
Uganda_R35_2015	Protein	AXB49954.1
Estonia_2014
Italy_26544_OG10_2010
Italy_47_Ss_2008_2008
Portugal_OURT_88.3_1988
Spain_E75_1975
Russia_Georgia_2007
Kenya_Tk1_2005	Protein Protein	AJL34050.1 AJL34050.1
Kenya_Bus_2006
Russia_Odintsovo_2014
Portugal_L60_1960
Portugal_NHV_1968
Russia_Kashino_2013
Benin_1997
South_Africa_KNP_Pretorisuskop_1996
South_Africa_MGR_Mkuzi_1979
Malawi_Lil_1983
Kenya_1950	Protein	AJL34050.1
Namibia_Warthog_1980
South_Africa_Warmbaths_1987
Malawi_Tengani_1962
China_HLJ_2018
China_LN_2018
China_wbBS01_2018
Ukraine_Kyiv_2014
Belgium_Etalle_wb_2018
Lithuania_LT14_2014
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence