Strain

Kenya_Tk1_2005 (Region: Kenya; Strain: Ken05/Tk1; Date: 2005)

Gene

BA71V-A240L

Description

Annotated in NCBI, similar to African swine fever virus E75 strain E75 of INSD accession FN557520

Location

KM111294.1(-):48150-48857

GenBank Accession

AJL34050.1

Full name

Thymidylate kinase       + More
dTMP kinase
Putative thymidylate kinase 251L
Probable thymidylate kinase

Alternative Name

dTMP kinase
dTMP kinase

CDS

ATGCGTGGAATACTTATTGCCATCGAAGGAATCAACGGTGTGGGGAAAAGCACACAGGCAATAAGGTTAAAAAATGCATTGGAAAATAAGAGATATGATGTGATATATATGCATTTTCCCAGTCCAAACACGGATACCGGTAAGCTTATATTGGATGTATTAAATAAAATCGTAAAAATGCCATCAGAACAATTGCATGAATTATTCACAAAACATCGATGTGAATTTACGGCAGAAATTGCAGCACTACTTAAACTAAATTTTATTGTAATTGTGGACCGTTATATTTGGTCTGGTTTGGCATATGCTCAAGCAGACAGAATAATGATTGATACCAAAAATACCTTCAACCCAGATTACACATTCTTTTTATCTTCAAACAAACCATTGAATGAAAAACCCTTACATTTACAACGATTATATGAAACAGAAGAAAAACAAGAAATAATTTTTACTCAATTTATTAATATCATAAATGAAGTACCTAAAGATAAATTTTGTGCTATTCCTGCAAATCTTAATAAGGAAATAATTGATAAGATCATTTTCTCAAAAACGATAAAAGTTTTTGAGAAAAACTTAAATTTAGACTATATTAAAATGTATGATGGTATGTATTTTAATATTCACGACTTAGATCTAATCCGTTTTGATTGGCAAAAATGCATTGAAGAAGATGATGGCATAGATGAGGAATACGGATTATAA

Protein

MRGILIAIEGINGVGKSTQAIRLKNALENKRYDVIYMHFPSPNTDTGKLILDVLNKIVKMPSEQLHELFTKHRCEFTAEIAALLKLNFIVIVDRYIWSGLAYAQADRIMIDTKNTFNPDYTFFLSSNKPLNEKPLHLQRLYETEEKQEIIFTQFINIINEVPKDKFCAIPANLNKEIIDKIIFSKTIKVFEKNLNLDYIKMYDGMYFNIHDLDLIRFDWQKCIEEDDGIDEEYGL

Function

Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.
Catalyzes the conversion of dTMP to dTDP.

Catalytic Activity

ATP + dTMP = ADP + dTDP
ATP + dTMP = ADP + dTDP

Similarity

Belongs to the thymidylate kinase family.

Keywords

ATP-binding Complete proteome Kinase Nucleotide biosynthesis Nucleotide-binding Transferase Reference proteome

Feature

chain Thymidylate kinase

Uniprot

A0A0C5AZC7 P0C8F9 A0A2Z5DGC8 E0WMH3 A0A2X0RUU2 P0C8G2 + More
A0A0N6WHZ1 A9JKX1 D4I5M3 A9JLM6 A0A0A1E300 P42490 P0C8G1 A0A1B7P8T1 A0A0L8HLB6 A0A0V0GB06 A0A220T6K7 R4FN90 C4WTA6 A0A0P4VS64 T1HFS6 A0A2A3EH77 A0A023F7Z7 A0A1B6EFI8 J9K470 F0UPZ4 C6HED4 A0A3M6YZH8 A0A224XKB1 A0A069DQQ3 A0A1J9PA31 D0AB90 A6QT32 C0NTM9 F2RNV5 L7X3W4 A0A212EPA4 A0A2A4JCZ2 A0A3M7DCF2 A0A163EG61 A0A2P2ENS6 A0A059JA89 A0A3M7HM84 F2PS14 A0A3M6ZXQ0 A0A3M7GXU5 G5E3F1 A0A3M6WU44 A0A0L8G043 M2LHW9 B6K7E8 A0A3M6Y233 A0A2H8TXE8 A0A1Z5T5P7 A0A3M7I0E2 A0A1Z5T7D8 A0A3M7IGY7 A0A3M7EVT3 E4URU8 A1CZX2 A0A017SNY0 A9UNQ0 A0A1E3BIW0 A1C4Z3 A0A3M7FAI3 A0A0H1BM71 A0A1F6IG40 A0A1Y1JRQ2 A0A0K8LAQ9 A0A2R5GGP8 A0A0S7DYG1 A0A162I9H9 A0A067REX9 Q6DF36 A0A2B7Z168 A0A1F5WCZ8 A0A0N0BJJ9 A0A397I3P2 A0A2B7WJM9 A0A2G9Z0Z9 A0A229XLS6 A0A3M7G0H7 A0A0J5SNS5 B0Y2U1 Q4WEL7 A0A229WNT2 A0A088AK34 Q91FS1 A0A117L7W8 A0A101DXR5 A0A2D8MM72 W8C5G7 A0A0J7KEL4 V8NMQ6 A0A370IUN5 A0A317W3D2 Q6NUD2 A0A1L8G4G1 A0A0U5GR06

EC Number

2.7.4.9
2.7.4.9

Pubmed

25645905 21470447 26618713 18198370 25406173 8393914 + More
11831707 28685222 27129103 25474469 29764372 26334808 19717792 22951933 23674305 22118469 23236275 21511999 18404212 24811710 18273011 27267057 27774985 27071652 24845553 20431018 26076650 16372009 11448171 17239238 26787827 29337314 24495485 24297900 27762356

EMBL

KM111294 AJL34050.1 AY261360 MH025916 MH025917 MH025918 + More
MH025919 MH025920 AXB49264.1 AXB49438.1 AXB49610.1 AXB49781.1 AXB49954.1 FR682468 CBW46701.1 MG939583 MG939587 MG939588 LS478113 AXZ95812.1 AXZ96000.1 AXZ96095.1 SPS73458.1 AY261366 KP055815 AKO62717.1 AM712239 CAN10134.1 FN557520 CBH29136.1 AM712240 CAN10383.1 KM262844 KM262845 KM102979 KX354450 AIY22226.1 AIY22385.1 AJZ77121.1 AOO54362.1 Z19544 U18466 AY261363 LGUA01000010 OAX85408.1 KQ417858 KOF90031.1 GECL01000844 JAP05280.1 KY747497 ASK51348.1 GAHY01001394 JAA76116.1 AK340538 BAH71126.1 GDKW01001174 JAI55421.1 ACPB03001438 ACPB03001439 KZ288256 PBC30569.1 GBBI01001330 JAC17382.1 GEDC01000601 JAS36697.1 ABLF02029968 DS990640 EGC47044.1 GG692424 EER41155.1 QWIJ01000749 QWIK01000314 RMX79079.1 RMY08443.1 GFTR01003511 JAW12915.1 GBGD01002927 JAC85962.1 LGRN01000307 OJD13368.1 FP102340 CBH09285.1 CH476655 EDN02674.1 GG663371 EEH05390.1 GG698478 EGD93011.1 KC469893 AGC92695.1 AGBW02013517 OWR43287.1 NWSH01001832 PCG69967.1 QWIN01000022 RMY61975.1 KV441026 KV440973 OAD67069.1 OAD78460.1 BFBS01016141 GBF62709.1 AOKY01000242 KDB24755.1 QWIP01000777 QWIO01000007 RMY54745.1 RMZ14481.1 DS995734 EGE04682.1 QWIL01000365 QWIM01000375 RMY19829.1 RMY35426.1 QWIQ01000120 RMZ05829.1 JP287915 AEQ17250.1 QWIJ01000460 RMX82097.1 KQ424852 KOF70367.1 KB445559 EMC93777.1 KE651168 EEB09452.1 QWIL01002081 QWIK01000751 QWIM01000919 QWIN01001560 QWIP01000008 RMX97075.1 RMY01535.1 RMY29847.1 RMY39376.1 RMY78729.1 GFXV01006666 MBW18471.1 MUNK01000116 OTA31342.1 QWIS01000245 QWIT01001168 RMZ00334.1 RMZ19041.1 MUNK01000103 OTA31939.1 QWIT01000397 RMZ24745.1 QWIQ01000623 RMY80698.1 DS989824 EFR01220.1 DS027686 EAW24292.1 KK088413 EYE98501.1 CH991543 EDQ92738.1 JXNT01000003 ODM20884.1 DS027004 EAW14761.1 QWIO01000763 RMY85842.1 LDEV01001147 KLJ12142.1 MFNV01000008 OGH21518.1 BDQF01000015 GAW83877.1 BBXM01000057 GAO84704.1 BEYU01000065 GBG29775.1 BCLY01000012 GAQ08937.1 AZGZ01000017 KZZ90403.1 KK852730 KDR17511.1 AAMC01060133 BC076907 CR761321 AAH76907.1 CAJ81735.1 PDNA01000009 PGH27150.1 MFHJ01000037 OGF73493.1 KQ435715 KOX79122.1 NKHV02000036 RHZ69567.1 PDNB01000270 PGG96717.1 PCRS01000004 PIP25152.1 NIDN01000021 OXN20832.1 QWIR01000013 RMY94437.1 KQ087363 KMK59102.1 DS499597 EDP51193.1 AAHF01000011 EAL85960.1 NKHT01000033 OXN28210.1 AF303741 LGFK01000007 KUK30488.1 LGEW01000003 KUK04860.1 NZOP01000017 MAM42036.1 GAMC01004884 JAC01672.1 LBMM01008713 KMQ88679.1 AZIM01002690 ETE63544.1 QFBF01000058 RDI77556.1 MSFL01000015 PWY79628.1 BC068663 AAH68663.1 CM004475 OCT78789.1 CDMC01000002 CEN60262.1

Proteomes

UP000105860 UP000000861 UP000141072 UP000000858 UP000241813 UP000130745 + More
UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 UP000266411 UP000000624 UP000000859 UP000091918 UP000053454 UP000217428 UP000015103 UP000242457 UP000007819 UP000008142 UP000002624 UP000182235 UP000009297 UP000001631 UP000009172 UP000007151 UP000218220 UP000077315 UP000245106 UP000024533 UP000009169 UP000011761 UP000001744 UP000194280 UP000002669 UP000006702 UP000019804 UP000001357 UP000094569 UP000006701 UP000036893 UP000051487 UP000027135 UP000008143 UP000224634 UP000053105 UP000215394 UP000223968 UP000215289 UP000053005 UP000001699 UP000002530 UP000215142 UP000005203 UP000001359 UP000254829 UP000247233 UP000186698 UP000054771

PRIDE

P0C8G2 P42490 P0C8G1

Pfam

PF02223 Thymidylate_kin

Interpro

IPR018095   Thymidylate_kin_CS
IPR039430   Thymidylate_kin_like_dom
IPR027417   P-loop_NTPase
IPR018094   Thymidylate_kinase

SUPFAM

SSF52540 SSF52540

ProteinModelPortal

A0A0C5AZC7 P0C8F9 A0A2Z5DGC8 E0WMH3 A0A2X0RUU2 P0C8G2 + More
A0A0N6WHZ1 A9JKX1 D4I5M3 A9JLM6 A0A0A1E300 P42490 P0C8G1 A0A1B7P8T1 A0A0L8HLB6 A0A0V0GB06 A0A220T6K7 R4FN90 C4WTA6 A0A0P4VS64 T1HFS6 A0A2A3EH77 A0A023F7Z7 A0A1B6EFI8 J9K470 F0UPZ4 C6HED4 A0A3M6YZH8 A0A224XKB1 A0A069DQQ3 A0A1J9PA31 D0AB90 A6QT32 C0NTM9 F2RNV5 L7X3W4 A0A212EPA4 A0A2A4JCZ2 A0A3M7DCF2 A0A163EG61 A0A2P2ENS6 A0A059JA89 A0A3M7HM84 F2PS14 A0A3M6ZXQ0 A0A3M7GXU5 G5E3F1 A0A3M6WU44 A0A0L8G043 M2LHW9 B6K7E8 A0A3M6Y233 A0A2H8TXE8 A0A1Z5T5P7 A0A3M7I0E2 A0A1Z5T7D8 A0A3M7IGY7 A0A3M7EVT3 E4URU8 A1CZX2 A0A017SNY0 A9UNQ0 A0A1E3BIW0 A1C4Z3 A0A3M7FAI3 A0A0H1BM71 A0A1F6IG40 A0A1Y1JRQ2 A0A0K8LAQ9 A0A2R5GGP8 A0A0S7DYG1 A0A162I9H9 A0A067REX9 Q6DF36 A0A2B7Z168 A0A1F5WCZ8 A0A0N0BJJ9 A0A397I3P2 A0A2B7WJM9 A0A2G9Z0Z9 A0A229XLS6 A0A3M7G0H7 A0A0J5SNS5 B0Y2U1 Q4WEL7 A0A229WNT2 A0A088AK34 Q91FS1 A0A117L7W8 A0A101DXR5 A0A2D8MM72 W8C5G7 A0A0J7KEL4 V8NMQ6 A0A370IUN5 A0A317W3D2 Q6NUD2 A0A1L8G4G1 A0A0U5GR06

PDB

2WWI E-value=1.24041e-12, Score=174

Ontologies

KEGG

vg:22220417 vg:1733213

GO

GO:0006233  P:dTDP biosynthetic process
GO:0004798  F:thymidylate kinase activity
GO:0005524  F:ATP binding
GO:0006235  P:dTTP biosynthetic process
GO:0016301  F:kinase activity
GO:0009041  F:uridylate kinase activity
GO:0005634  C:nucleus
GO:0005829  C:cytosol
GO:0005737  C:cytoplasm
GO:0005739  C:mitochondrion
GO:0006227  P:dUDP biosynthetic process
GO:0004550  F:nucleoside diphosphate kinase activity

Subcellular Location

From MSLVP

Single-Pass Membrane

Topology

Length:

235

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.17619

Exp number, first 60 AAs:

0.00157

Total prob of N-in:

0.02585

Length:

235

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.17619

Exp number, first 60 AAs:

0.00157

Total prob of N-in:

0.02585

outside

1 - 235

outside

1 - 235

Population Genetic Test Statistics

Pi

0.0884416

Theta

0.0967098

Tajima's D

-0.6238942

CLR

0.30277

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971
China_AnhuiXCGQ_2018
China_ASFV-SY18_2018
Poland_Pol16_20186_o7_2016-2017
Poland_Pol16_20538_o9_2016-2017
Poland_Pol16_20540_o10_2016-2017
Poland_Pol16_29413_o23_2016-2017
Poland_Pol17_03029_C201_2016-2017
Poland_Pol17_04461_C210_2016-2017
Poland_Pol17_05838_C220_2016-2017
Poland_ASFV_POL_Podlaskie_2015
Uganda_R8_2015	Protein	AXB49264.1
Uganda_R7_2015	Protein	AXB49438.1
Uganda_R25_2015	Protein	AXB49610.1
Uganda_N10_2015	Protein	AXB49781.1
Uganda_R35_2015	Protein	AXB49954.1
Estonia_2014
Italy_26544_OG10_2010
Italy_47_Ss_2008_2008
Portugal_OURT_88.3_1988
Spain_E75_1975
Russia_Georgia_2007
Kenya_Tk1_2005	Protein Protein	AJL34050.1 AJL34050.1
Kenya_Bus_2006
Russia_Odintsovo_2014
Portugal_L60_1960
Portugal_NHV_1968
Russia_Kashino_2013
Benin_1997
South_Africa_KNP_Pretorisuskop_1996
South_Africa_MGR_Mkuzi_1979
Malawi_Lil_1983
Kenya_1950	Protein	AJL34050.1
Namibia_Warthog_1980
South_Africa_Warmbaths_1987
Malawi_Tengani_1962
China_HLJ_2018
China_LN_2018
China_wbBS01_2018
Ukraine_Kyiv_2014
Belgium_Etalle_wb_2018
Lithuania_LT14_2014
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence