ASFVdb The African Swine Fever Virus Database
Strain
Russia_Georgia_2007 (Region: Russia;  Strain: Georgia 2007/1;  Date: 2007)
Gene
A238L
Description
Annotated in NCBI,  contains IkB-like ankyrin repeats
GenBank Accession
Full name
IkB-like protein      
Alternative Name
Ankyrin repeat domain-containing protein A238L
p28
 

Sequence

CDS
ATGTTTCCAGAAAGGGAGATAGAAAACTTGTTTGTTAAATGGATTAAAAAACATATTAGAAATGGGAATCTTACACTATTTGAGGAATTTTTTAAAACAGATCCGTGGATTGTCAATAGATGCGATAAAAATGGATCCTCGGTATTCATGTGGATATGCATCTACGGACGTATAGACTTTTTAAAATTTCTTTTTGAACAAGAATCTTATCCTGGAGAAATAATTAACCCTCATAGGAGGGATAAAGATGGAAACTCTGCTTTACATTATTTAGCTGAGAAAAAAAATCATTTAATCCTGGAAGGGGTGTTGGGCTATTTCGGAAAAAATGGGACCAGAATTTGTTTACCGAATTTTAATGGGATGACTCCTGTGATGAAGGCCGCAATACGGGGCCGTTCTTTAAATATGCTTTCTCTCATAAAATTTGGAGCAGATCCGACTCAAAAAGACTATCATAGAGGTTTTACTGCTTGGGACTGGGCAGTCTTTACAGGAAACATGGAGTTAGTCAAATCTCTTAACCATGACTACCAAAACCTCTCTACATGCATTTCCCTCTTTACAAGCTGGATGTTTTCCACCGGTGGTTTAAGAAAAAGCCCAAAATTATTATTACTGGCTGTAAACATAATGTCTATGAAAAACTTCCTGAACAGAATCCAAAGTTCCTGTGTGTAA
Protein
MFPEREIENLFVKWIKKHIRNGNLTLFEEFFKTDPWIVNRCDKNGSSVFMWICIYGRIDFLKFLFEQESYPGEIINPHRRDKDGNSALHYLAEKKNHLILEGVLGYFGKNGTRICLPNFNGMTPVMKAAIRGRSLNMLSLIKFGADPTQKDYHRGFTAWDWAVFTGNMELVKSLNHDYQNLSTCISLFTSWMFSTGGLRKSPKLLLLAVNIMSMKNFLNRIQSSCV

Summary

Function
I-kappa-B- (IkB)-like protein that inhibits the binding of NF-kappa-B to DNA, thereby downregulating proinflammatory cytokine production (By similarity). Forms a heterodimer with the NF-kappa-B subunit RELA/p65 and prevents the activation of the NF-kappa-B transcription factor (PubMed:10934190). Also inhibits the host calcineurin phosphatase activity, which is required for the induction of nuclear factor of activated T cells(NFAT)-dependent immune response genes (By similarity). Inhibits calcineurin function, which is required for the induction of nuclear factor of activated T cells (NFAT)-dependent immune response genes (By similarity). Prevents the binding of substrates to calcineurin without affecting the phosphatase activity (By similarity). Does not contain the serine residues that are phosphorylated by host IkB kinase and thus is not degraded following stimulation of the NFkB pathway (PubMed:10934190).
IkB-like protein that inhibits the binding of NF-kappa-B to DNA, thereby downregulating proinflammatory cytokine production (By similarity). Forms a heterodimer with the NF-kappa-B subunit RELA/p65 and prevents the activation of the NF-kappa-B transcription factor (By similarity). Inhibits calcineurin function, which is required for the induction of nuclear factor of activated T cells (NFAT)-dependent immune response genes. Prevents the binding of substrates to calcineurin without affecting the phosphatase activity (By similarity). Does not contain the serine residues that are phosphorylated by host IkB kinase and thus is not degraded following stimulation of the NFkB pathway (By similarity).
IkB-like protein that inhibits the binding of NF-kappa-B to DNA, thereby downregulating proinflammatory cytokine production (PubMed:8970976). Forms a heterodimer with the NF-kappa-B subunit RELA/p65 and prevents the activation of the NF-kappa-B transcription factor (By similarity). Inhibits calcineurin function, which is required for the induction of nuclear factor of activated T cells (NFAT)-dependent immune response genes (PubMed:23468591, PubMed:9677199). Prevents the binding of substrates to calcineurin without affecting the phosphatase activity (PubMed:23468591). Does not contain the serine residues that are phosphorylated by host IkB kinase and thus is not degraded following stimulation of the NFkB pathway (By similarity).
Subunit
Interacts with host PPIA (By similarity). Interacts with host PPP3CA/Calcineurin (PubMed:18261759). Interacts with host RELA/p65; interaction of the 32 kDa form with host RELA results in the formation of a stable complex with NF-kappa-B (PubMed:10934190). Interacts with host PPP3R1 (By similarity). Interacts with host EP300; this interaction inhibits the association of host EP300 with host RELA, JUN and NFATC2 (PubMed:16365438).
Interacts with host PPIA. Interacts with host PPP3CA/Calcineurin (By similarity). Interacts with host RELA/p65; interaction of the 32 kDa form with host RELA results in the formation of a stable complex with NF-kappa-B (By similarity). Interacts with host PPP3R1 (By similarity). Interacts with host EP300; this interaction inhibits the association of host EP300 with host RELA, JUN and NFATC2 (By similarity).
Interacts with host PPIA (PubMed:9677199). Interacts with host PPP3CA/Calcineurin (PubMed:9677199, PubMed:11000210, PubMed:23468591). Interacts with host RELA/p65; interaction of the 32 kDa form with host RELA results in the formation of a stable complex with NF-kappa-B (By similarity). Interacts with host PPP3R1 (PubMed:23468591). Interacts with host EP300; this interaction inhibits the association of host EP300 with host RELA, JUN and NFATC2 (By similarity).
Similarity
Belongs to the asfivirus A238L family.
Keywords
ANK repeat   Complete proteome   Host cytoplasm   Host nucleus   Host-virus interaction   Inhibition of host NF-kappa-B by virus   Reference proteome   Repeat   Ubl conjugation   3D-structure  
Feature
chain  IkB-like protein
EMBL
FR682468    CBW46705.1    KU752596    KU752597    KU752598    KU752599    + More
KU752600    KU752601    KU752602    KU752603    KU752604    KU752605    KU752606    KU752607    KU752608    KU752609    KU752610    KU752611    KU752612    KU752613    KU752614    KU752615    KU752616    KU752617    KU752618    KU752619    KU752620    KU752621    KU752622    KU752623    KU752624    KU752625    KU752626    KU752627    KU752628    KU752629    KU752630    KU752631    KU752632    KU752633    KU752634    KU752635    KU752636    KU752637    KU752638    KU752639    KU752640    KU752641    KU752642    KU752643    KU752644    KU752645    KU752646    KU752647    KU752648    KU752649    KU752650    KU752651    KU752652    KU752653    KU752654    KU752655    KU752656    KU752657    KU752658    KU752659    KU752660    KU752661    KU752662    LS478113    ANC98785.1    ANC98786.1    ANC98787.1    ANC98788.1    ANC98789.1    ANC98790.1    ANC98791.1    ANC98792.1    ANC98793.1    ANC98794.1    ANC98795.1    ANC98796.1    ANC98797.1    ANC98798.1    ANC98799.1    ANC98800.1    ANC98801.1    ANC98802.1    ANC98803.1    ANC98804.1    ANC98805.1    ANC98806.1    ANC98807.1    ANC98808.1    ANC98809.1    ANC98810.1    ANC98811.1    ANC98812.1    ANC98813.1    ANC98814.1    ANC98815.1    ANC98816.1    ANC98817.1    ANC98818.1    ANC98819.1    ANC98820.1    ANC98821.1    ANC98822.1    ANC98823.1    ANC98824.1    ANC98825.1    ANC98826.1    ANC98827.1    ANC98828.1    ANC98829.1    ANC98830.1    ANC98831.1    ANC98832.1    ANC98833.1    ANC98834.1    ANC98835.1    ANC98836.1    ANC98837.1    ANC98838.1    ANC98839.1    ANC98840.1    ANC98841.1    ANC98842.1    ANC98843.1    ANC98844.1    ANC98845.1    ANC98846.1    ANC98847.1    ANC98848.1    ANC98849.1    ANC98850.1    ANC98851.1    SPS73462.1    MG939583    MG939587    MG939588    AXZ95815.1    AXZ96003.1    AXZ96098.1    FN557520    CBH29140.1    AM712240    CAN10387.1    AF014475    AF014476    AF014477    AF014480    AF014482    AF014485    FJ972623    FJ972624    KM262844    KM262845    KP055815    KX354450    AAB71357.1    AAB71358.1    AAB71359.1    AAB71362.1    AAB71364.1    AAB71367.1    ACS29491.1    ACS29492.1    AIY22230.1    AIY22389.1    AKO62721.1    AOO54366.1    U18466    AM712239    CAN10138.1    KM102979    AJZ77124.1    AF014483    AAB71365.1    AF014472    AAB71354.1    AY261366    AF014474    AAB71356.1    AF014473    AAB71355.1    AY261363    AF014481    AAB71363.1    AF014484    AAB71366.1    AF014478    AAB71360.1    AY261360    KM111294    AJL34053.1    KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    AJL34218.1    AXB49268.1    AXB49442.1    AXB49614.1    AXB49785.1    AXB49958.1    AF014479    AY261361   
Pfam
PF00023   Ank
Interpro
IPR002110   Ankyrin_rpt
IPR020683   Ankyrin_rpt-contain_dom
IPR036770   Ankyrin_rpt-contain_sf
SUPFAM
SSF48403   SSF48403
Gene 3D
PDB
5FFJ     E-value=7.2654e-14,     Score=191

Ontologies

KEGG

Subcellular Location

From MSLVP
Nucleus
From Uniprot
Host nucleus   Binding to host PPP3CA/Calcineurin may mask the second nuclear localization signal thereby contributing to the cytoplasmic retention of A238L.   With evidence from 5 publications.
Host cytoplasm   Binding to host PPP3CA/Calcineurin may mask the second nuclear localization signal thereby contributing to the cytoplasmic retention of A238L.   With evidence from 5 publications.

Topology

Length:
226
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.0742
Exp number, first 60 AAs:
0.01216
Total prob of N-in:
0.02268
outside
1  -  226
 
 

Population Genetic Test Statistics

Pi
0.0747625
Theta
0.0853322
Tajima's D
-0.4251046
CLR
0
Interpretation
No evidence of Selection

Multiple alignment of Orthologues

 
 

Gene Tree

 
 

Orthologous in Strains

Strain Availability Status Gene
Spain_BA71_1971 Protein
AKO62721.1
China_AnhuiXCGQ_2018 Protein
AYW34011.1
China_ASFV-SY18_2018 Protein
AKO62721.1
Poland_Pol16_20186_o7_2016-2017 Protein
AXZ95815.1
Poland_Pol16_20538_o9_2016-2017    
Poland_Pol16_20540_o10_2016-2017    
Poland_Pol16_29413_o23_2016-2017    
Poland_Pol17_03029_C201_2016-2017 Protein
AXZ96003.1
Poland_Pol17_04461_C210_2016-2017 Protein
AXZ96098.1
Poland_Pol17_05838_C220_2016-2017    
Poland_ASFV_POL_Podlaskie_2015    
Uganda_R8_2015    
Uganda_R7_2015    
Uganda_R25_2015    
Uganda_N10_2015    
Uganda_R35_2015    
Estonia_2014 Protein
SPS73462.1
Italy_26544_OG10_2010 Protein
AJZ77124.1
Italy_47_Ss_2008_2008 Protein
AOO54366.1
Portugal_OURT_88.3_1988 Protein
CAN10387.1
Spain_E75_1975 Protein
CBH29140.1
Russia_Georgia_2007 Protein
CBW46705.1
Kenya_Tk1_2005    
Kenya_Bus_2006    
Russia_Odintsovo_2014    
Portugal_L60_1960 Protein
AIY22230.1
Portugal_NHV_1968 Protein
AIY22389.1
Russia_Kashino_2013    
Benin_1997 Protein
CAN10138.1
South_Africa_KNP_Pretorisuskop_1996    
South_Africa_MGR_Mkuzi_1979    
Malawi_Lil_1983    
Kenya_1950    
Namibia_Warthog_1980    
South_Africa_Warmbaths_1987    
Malawi_Tengani_1962    
China_HLJ_2018 Protein
QBH90527.1
China_LN_2018 Protein
QBH90712.1
China_wbBS01_2018 Protein
QDL88070.1
Ukraine_Kyiv_2014 Protein
Protein
QED21595.1
QED21595.1
Belgium_Etalle_wb_2018 Protein
QED90482.1
Lithuania_LT14_2014 Protein
QEY87845.1
South_Africa_1985    
South_Africa_2_2008    
Zambia_LIV_1983    
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