Strain

Malawi_Lil_1983 (Region: Malawi, Chalaswa; Strain: Malawi Lil-20/1 (1983); Date: 1983)

Gene

Helicase

Description

Annoated by sequence mapping, Member of helicase superfamily II group including vaccinia D11L and D6R, and ASFV pD1133L

Location

AY261361.1(-):155859-157979

GenBank Accession

CAN10221.1

Full name

Putative ATP-dependent RNA helicase Q706L

CDS

ATGTCTTGCGTCCACAACAACACCTCCTTCCCCGTCCAAATAGAAGCATACCTAAAGGAGGTATTTGAAAAATATAAAGAGCTTCAGGAAAGCAAAGACACCTCACTCACTGCCCGTTTTGCACGAGTACTGAAATACTACCAGTTTTTAATTTACTCCGCCTTTTCGGATCCTAAATTTGGGATAGGCCAGGGGGAAAACACACGGGGCCTCCTCATCTATCATCAGATGGGCATGGGGAAAACCATTTTGAGCCTTTCGCTTGCTATTTCGCTTTCACATATTTACAACCCGATTTTGATCGCACCCAAATCGTTGCACTCAAACTTCCAACAGTCTTTGCTAAAGCTTATTAAACTTTTATATCCGGAAACGACGGATCACTCCAAAGAGCTACAAAAAATCTCGCGGCGCTTTAGGTTTGTGTCCCTAGACGCTTACAACATGGGGCAGCAGATCATAAAGGCGGGAGGGTCCCTAAACGGATGCCTGCTTATCGTTGATGAGGCACACAACCTCTTTAGAGGAATTATCAATAGCGCCAATGATAAAACAAATGCCCGCCAGCTGTACAATAATATTATGCAAGCGAAAAATATTCGTATCCTGTTTTTAACAGGGACCCCGTGTTCGAAGGACCCTTTTGAAATGGTGCCCTGTTTTAATATGCTGAGCGGGCGAATTTTATTACCTCTTCACTATGAAAGATTCTACACCGCTTATGTCAATAAAACAACGAATAGTCCTTTAAACGCGGACAAGCTGCTAAACCGCCTAGTGGGTATGATAAGCTATGCTGGAAACCAAAATGAACTCAACAAGCTATTCCCTACAGAACTTCCTCTTATTATAGAAAAGGTTGAAATGTCACCCGAGCAGTATAGGCAATATCTGTTGGCTCGGGACGTTGAAAACGCGGAAAAGCACGCCAGCTCGGGCATGTATGAAAAAATGAATACCGCCGCCCTCTGCCTGCCAGGATCGGAGCAAGAGTCAGGCTCCTCCTACTATGTGCGTTCCCGTATGATCAGCATCTTCGCGTCCGAAATGCTAACAGTAAAGGAGGACGAAAAACTATCAGAGGCTGTTCAGCAGCTGCCCAAAGAGGCCTTCACTGAAAATTCTAGTCCCAAAATAGCATGCATGCTAAAAAATATTAAAACATCTCCGGGTCCTGTGCTCATTTACTCTCAGTTTGTGGAATTGGGCCTTCACGTAGTGGCCCGCTTTTTAGAAATAGAGGGCTATCAGTGCCTTCAGCCCCTAAAGGTACTAGAAGAAGGGCACAACACCATCCTCCTTCATAAGGACGGAAAAGATCTTATGGTGAAAAACTTTGCGGAAGATGAGCCGACCCATACGTTAGTCCTGTCCTCCAAAATAACACGATTCACTCTTATTACAGGGAAAATTTTATCTAAAGAGCGCGACATGATCCAACAGCTTTGGAATTCGCCGCTGAATATACACGGAGAGGTGATTAAAATTTTACTCGTCTCTAAAACGGGGGCTGAGGGATTGGATCTCAAATACGGGCGGCAGGTTCATATCCTAGAGCCGTACTGGGATAAGGCCCGAGAGGATCAAGTGAAGGCGCGCATTATACGCATTGGTAGCCACGATGCGCTACCGCCTGAGGAGAAAACCGTACAGCCTTTTTTGTACATCGCGGTGGCTAATCAAAAAATGTTTTATAGTATTCCCGAAGGCAGTCAGGAACAAAAAACGATTGATGAGCGTTTTCACGAAAGGGGGCTTGAAAAAAGCCATCTTAATTCTGCTTTCCGTGATTTACTAAAAAGGGCAGCCATTGAATGTGCCTTCAACGGCGAAAGTGGCTGCCTGATGTGTCAGCCCACAAATGCTCTCCTTTTTCATGAAAACTTTGAACGCGATTTACGTTTGCCCAATCCCTGCCAACCGCTTGTAAAAACCGAAGTAAAAGCCTATAGCATATCCTACGAGGGCAAACAGTTTTTTTACCAAAAAAACAAAGAGGTAGGATTAGGCTATACCTTTTATGAATACAATCCCATCATTAAGGCATATATTGAAATTAAACCTTCTAATCCTTTATATATAAAGTTAATAAAACATGTCCAGGCCGGAACAACAGTTTAA

Protein

MSCVHNNTSFPVQIEAYLKEVFEKYKELQESKDTSLTARFARVLKYYQFLIYSAFSDPKFGIGQGENTRGLLIYHQMGMGKTILSLSLAISLSHIYNPILIAPKSLHSNFQQSLLKLIKLLYPETTDHSKELQKISRRFRFVSLDAYNMGQQIIKAGGSLNGCLLIVDEAHNLFRGIINSANDKTNARQLYNNIMQAKNIRILFLTGTPCSKDPFEMVPCFNMLSGRILLPLHYERFYTAYVNKTTNSPLNADKLLNRLVGMISYAGNQNELNKLFPTELPLIIEKVEMSPEQYRQYLLARDVENAEKHASSGMYEKMNTAALCLPGSEQESGSSYYVRSRMISIFASEMLTVKEDEKLSEAVQQLPKEAFTENSSPKIACMLKNIKTSPGPVLIYSQFVELGLHVVARFLEIEGYQCLQPLKVLEEGHNTILLHKDGKDLMVKNFAEDEPTHTLVLSSKITRFTLITGKILSKERDMIQQLWNSPLNIHGEVIKILLVSKTGAEGLDLKYGRQVHILEPYWDKAREDQVKARIIRIGSHDALPPEEKTVQPFLYIAVANQKMFYSIPEGSQEQKTIDERFHERGLEKSHLNSAFRDLLKRAAIECAFNGESGCLMCQPTNALLFHENFERDLRLPNPCQPLVKTEVKAYSISYEGKQFFYQKNKEVGLGYTFYEYNPIIKAYIEIKPSNPLYIKLIKHVQAGTTV

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Similarity

Belongs to the DEAD box helicase family. DEAH subfamily.

Keywords

ATP-binding Complete proteome Helicase Hydrolase Nucleotide-binding Reference proteome

Feature

chain Putative ATP-dependent RNA helicase Q706L

Uniprot

A9JLA5 D4I5E5 A9JM41 A0A0A1DYI9 Q89581 E0WM71 + More
A0A2X0TKN3 P0C9B3 A0A0C5AWF0 A0A0C5AWV7 P0C9B2 P0C9B1 Q89926

Pubmed

18198370 25406173 26618713 11831707 21470447 25645905 + More
8376971 8397501 8021596

EMBL

AM712239 CAN10221.1 FN557520 CBH29221.1 AM712240 CAN10470.1 + More
KM262844 KM262845 KM102979 KP055815 KX354450 AIY22312.1 AIY22470.1 AJZ77053.1 AKO62802.1 AOO54488.1 U18466 X69952 FR682468 CBW46787.1 MG939583 MG939588 LS478113 AXZ95894.1 AXZ96177.1 SPS73544.1 AY261360 KM111294 AJL34134.1 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34299.1 AXB49350.1 AXB49524.1 AXB49696.1 AXB49867.1 AXB50040.1 AY261366 AY261363 X72952 M88275 X71982 AY261361

Proteomes

UP000130745 UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 + More
UP000241813 UP000266411 UP000000624 UP000141072 UP000000861 UP000105860 UP000101566 UP000000858 UP000000859 UP000000860

PRIDE

P0C9B2

Pfam

PF00176 SNF2_N
PF00271 Helicase_C

Interpro

IPR014001   Helicase_ATP-bd
IPR027417   P-loop_NTPase
IPR038718   SNF2-like_sf
IPR001650   Helicase_C
IPR000330   SNF2_N

SUPFAM

SSF52540 SSF52540

Gene 3D

3.40.50.10810

ProteinModelPortal

A9JLA5 D4I5E5 A9JM41 A0A0A1DYI9 Q89581 E0WM71 + More
A0A2X0TKN3 P0C9B3 A0A0C5AWF0 A0A0C5AWV7 P0C9B2 P0C9B1 Q89926

PDB

6RFL E-value=2.8265e-14, Score=194

Ontologies

KEGG

vg:22220351

GO

GO:0005524 F:ATP binding
GO:0004386 F:helicase activity

Subcellular Location

From MSLVP

Nucleus

Topology

Length:

706

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

3.40418000000001

Exp number, first 60 AAs:

0.02038

Total prob of N-in:

0.16711

outside

1 - 706

Population Genetic Test Statistics

Pi

0.0257098

Theta

0.0409595

Tajima's D

-1.1663026

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62802.1
China_AnhuiXCGQ_2018	Protein	AYW34092.1
China_ASFV-SY18_2018	Protein	AKO62802.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95894.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10221.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10221.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10221.1
Poland_Pol17_03029_C201_2016-2017	Protein	CAN10221.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96177.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10221.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10221.1
Uganda_R8_2015	Protein	AXB49350.1
Uganda_R7_2015	Protein	AXB49524.1
Uganda_R25_2015	Protein	AXB49696.1
Uganda_N10_2015	Protein	AXB49867.1
Uganda_R35_2015	Protein	AXB50040.1
Estonia_2014	Protein	SPS73544.1
Italy_26544_OG10_2010	Protein	AJZ77053.1
Italy_47_Ss_2008_2008	Protein	AOO54488.1
Portugal_OURT_88.3_1988	Protein	CAN10470.1
Spain_E75_1975	Protein	CBH29221.1
Russia_Georgia_2007	Protein	CBW46787.1
Kenya_Tk1_2005	Protein	AJL34134.1
Kenya_Bus_2006	Protein	AJL34299.1
Russia_Odintsovo_2014	Protein	CAN10221.1
Portugal_L60_1960	Protein	AIY22312.1
Portugal_NHV_1968	Protein	AIY22470.1
Russia_Kashino_2013	Protein	CAN10221.1
Benin_1997	Protein Protein	CAN10221.1 CAN10221.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10221.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10221.1
Malawi_Lil_1983	Protein	CAN10221.1
Kenya_1950	Protein	CAN10221.1
Namibia_Warthog_1980	Protein	CAN10221.1
South_Africa_Warmbaths_1987	Protein	CAN10221.1
Malawi_Tengani_1962	Protein	CAN10221.1
China_HLJ_2018	Protein	QBH90609.1
China_LN_2018	Protein	QBH90794.1
China_wbBS01_2018	Protein	QDL88152.1
Ukraine_Kyiv_2014	Protein Protein	QED21731.1 QED21732.1
Belgium_Etalle_wb_2018	Protein	QED90564.1
Lithuania_LT14_2014	Protein	QEY87927.1
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence