ASFVdb The African Swine Fever Virus Database
Strain
South_Africa_2_2008 (Region: South Africa;  Strain: RSA_2_2008;  Date: 2008)
Gene
NifS-like protein
Description
With frameshift mutation but translatable,  similar to pyridoxal phosphate-dependent aminotransferases of class V
GenBank Accession
Full name
NifS-like protein      
 

Sequence

CDS
ATGGCCTCCATTCTCACGCTGGACGGGCTATATGCAGAGGTTCCAAAATTCTTACCAGAGGCGTTACGAGAGGGATGTGCTGGCAAGAATCCTCTATGCTTTTATATTCAACAAATTTTATATTTATTGGGATGTGTTGGTATCGAGTATCATGTTCTTTTTACCAGCAGCTCCGAGGAAGCAAATACTCATATGTTCATGGCCGCCGTGCGTCGCCATTTGCTGCGGACGCAGCAAAGGCCTCATGTCATTATCGGAGCAGCCGAGCCCCCTACCGTCACCGAATGTGTGCAGGCATTGGCGCAGGAAAAACGCTGCGTATACACCATCATCCCCCTACAAAATTTTGCAATACATCCTGTTGCGGTATACGATGCCATACAAAGCAATACCTGCTTACCGTGCATTTCGGGCACCAATGCTGTTGTCAAAACGTTCAACAAACTCCAGGACATTACCAAAGTGTTACGAGGTATTCCCCTGCACTCGGAAGTGTGTGTTCTTGTTTACCAAGGATGTATTATACAAAATCCGCCCGCTGTTACTTTTTCAATATATACTCTCTACGGCTTCTTGGGAGTCGGTGTCTTGGGAATGCAGAAAAAGGTCATGCAAGGATTGGGGCCGCTCATTTTTGGAGGAGGGCTGCGAGGCGGAAGCCCTATTATACCCGGAATTCATGCCATGTATACAACGCTATCCCAGCAAAGGCCTTCCATGCAAAAAATATATACAATACATACGCTGTTTATGCAAACTTTACAAAAACATCAGCATGTATATCTACCCATACGGGGCGTGTCTACAAAAGACATGTCATGTCTACAAAAGACATACCCTCTACCGACATGCCTGTTGGCCCGAAGGAACTCCCGGGCTATATTTTATTTACCGTTGGTCGTCGCGCCGAGGAGCTACAAAAAAAAATTTTCACTATATTTATTATACAGGTTGGCCGTATTATTGTCTCACAAGAGATACTGTTTCGTATCAAAATACCCCAGAAATACTGGGAGACATTATTGTTCATCCAATTACGAGATATTTTGTCCAAAGAGGATATACAGAGAGTTATGGTTGTTTTGTTGCATTTACATACCATCACTCCTCGTGGCTCTCTTCCTCCTCCGAGCTACTCTTCTTCTTTTTCTT
Protein
MASILTLDGLYAEVPKFLPEALREGCAGKNPLCFYIQQILYLLGCVGIEYHVLFTSSSEEANTHMFMAAVRRHLLRTQQRPHVIIGAAEPPTVTECVQALAQEKRCVYTIIPLQNFAIHPVAVYDAIQSNTCLPCISGTNAVVKTFNKLQDITKVLRGIPLHSEVCVLVYQGCIIQNPPAVTFSIYTLYGFLGVGVLGMQKKVMQGLGPLIFGGGLRGGSPIIPGIHAMYTTLSQQRPSMQKIYTIHTLFMQTLQKHQHVYLPIRGVSTKDMSCLQKTYPLPTCLLARRNSRAIFYLPLVVAPRSYKKKFSLYLLYRLAVLLSHKRYCFVSKYPRNTGRHYCSSNYEIFCPKRIYRELWLFCCIYIPSLLVALFLLRATLLLFL

Summary

Cofactor
pyridoxal 5'-phosphate
Similarity
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.
Keywords
Complete proteome   Pyridoxal phosphate   Reference proteome  
Feature
chain  NifS-like protein
Pfam
PF00266   Aminotran_5
Interpro
IPR015421   PyrdxlP-dep_Trfase_major
IPR015422   PyrdxlP-dep_Trfase_dom1
IPR015424   PyrdxlP-dep_Trfase
IPR000192   Aminotrans_V_dom
SUPFAM
SSF53383   SSF53383
Gene 3D
PDB
4R5F     E-value=0.126582,     Score=84

Ontologies

KEGG

Subcellular Location

From MSLVP
Multi-Pass Membrane

Topology

Length:
384
Number of predicted TMHs:
2
Exp number of AAs in TMHs:
52.12574
Exp number, first 60 AAs:
4.54504
Total prob of N-in:
0.08743
outside
1  -  178
TMhelix
179  -  198
inside
199  -  357
TMhelix
358  -  380
outside
381  -  384
 
 

Population Genetic Test Statistics

Pi
0.0490376
Theta
0.047786
Tajima's D
-0.6168494
CLR
0.07855
Interpretation
No evidence of Selection

Multiple alignment of Orthologues

 
 

Gene Tree

 
 

Orthologous in Strains

Strain Availability Status Gene
Spain_BA71_1971    
China_AnhuiXCGQ_2018    
China_ASFV-SY18_2018    
Poland_Pol16_20186_o7_2016-2017    
Poland_Pol16_20538_o9_2016-2017    
Poland_Pol16_20540_o10_2016-2017    
Poland_Pol16_29413_o23_2016-2017    
Poland_Pol17_03029_C201_2016-2017    
Poland_Pol17_04461_C210_2016-2017    
Poland_Pol17_05838_C220_2016-2017    
Poland_ASFV_POL_Podlaskie_2015    
Uganda_R8_2015    
Uganda_R7_2015    
Uganda_R25_2015    
Uganda_N10_2015    
Uganda_R35_2015    
Estonia_2014    
Italy_26544_OG10_2010    
Italy_47_Ss_2008_2008    
Portugal_OURT_88.3_1988    
Spain_E75_1975    
Russia_Georgia_2007    
Kenya_Tk1_2005    
Kenya_Bus_2006    
Russia_Odintsovo_2014    
Portugal_L60_1960    
Portugal_NHV_1968    
Russia_Kashino_2013    
Benin_1997    
South_Africa_KNP_Pretorisuskop_1996    
South_Africa_MGR_Mkuzi_1979    
Malawi_Lil_1983    
Kenya_1950    
Namibia_Warthog_1980    
South_Africa_Warmbaths_1987    
Malawi_Tengani_1962    
China_HLJ_2018    
China_LN_2018    
China_wbBS01_2018    
Ukraine_Kyiv_2014    
Belgium_Etalle_wb_2018    
Lithuania_LT14_2014    
South_Africa_1985 Protein
QGM12939.1
South_Africa_2_2008 Protein
Protein
Protein
CAN10223.1
QGM12865.1
QGM12865.1
Zambia_LIV_1983 Protein
QGM12758.1
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