Strain

Zambia_LIV_1983 (Region: Zambia;  Strain: LIV_5_40;  Date: 1983)

Gene

pNP868R

Description

Annotated in NCBI,  NP868R

Location

MN318203.1(+):130382-133030

GenBank Accession

QGM12743.1

Full name

Probable mRNA-capping enzyme       + More

CDS

ATGGCTTCTTTACATATTTTACTGGCACGATATCAGAGGTGCTTTATTGTCCAGTCTCTTACAAATACTACTATTGTACTTGCAATACGTTTTCAACAGATATATTTTTTATTATTCAAAACCGTATATGTGGCACTTGTGGCACAAGAGATCCCTACCACCATCTCTCACAGCATCCGCTGCATCAAAAAAGTTCACCATGCAAACCACTGCCGGGAAAAAATTTTGCCGTCGGAGAATCTTTACTTCAAAAAACAGCCTCTCATGTTTTTTATGTTTTCAGAGCCTGCATCTCTGGGCTGTATGGTCTCGCTGGCCATCGAGCAGCCCATTCGTATATTTATCTTGGACTCCTCCGTTCTCGTTCGGCTCAAAAATCGTACGACCTTTCGGGTATCTGTACTTTGGAAAATACAGCTTACCATTGTATACCAGCTGTTGGGAAGCGAGGTCTCTGCAAAACTTGCCGCCTTCAAAACGCTTCTGTTTGTCACCCCAGAGCAACAAACGACAAAAAATATGTTGTCGTTATTATACCCAGATGTCGAATATCTTTACGAAATACAAATACAGTATACAGGAAAGCCCGAATCCCTATCGGCGGCAGATGTTATACAAATTACAAACACGGTGTTGTCACTTATTTCTCCGAACCATTTATTGCTATCAGCCTACCACCAGGCCATTGTATTCATTGCCTCCCATATACTGTCCTCAGAAATCCTTCTTGCTCGTATTATGTGCGGGAAGTGGGGGCTTATACGCCTCCTCCCCCAGGTGCAATCCATGTCCAAAGCGGATTACATGCAATTTTATCCGCCCGTTGGCTACTATGTATCGGACAAAGCAGATGGAATTACAGGCATCGCCGTCATTCAGGACACGCAAATTTATGTGGTTGCAGACCAGTTATACAGCCTACGTACCACCGGCATTGTACCCCTTATACCAACCATTTTGGACGGTGTATTTATGCCTGCAAAAAAAGAATTTTATGGGTTTGCCGTCATCATGTATGTGGGCAATCTGTTGTCGCAACAGGGGTTTGCAACAAGAATTGTGTCTTTATGCAAGGGCATTATACTCTTACAAGCGTTTATCATACAAGCAGAAATGTACCCCTTTATTTCGCTATCAAGTGCAGATCCCAACGTGCTCCTCAAAAACTTTGCAAGCATTTTTATGCAAAAAACTCGCCCATATTCTATTGTTGGCATCATTTTACTACAACCTGGCAATTCTTATCTATATACAAATACCTTTATGTGGAAGCCTACCTGGGATATCACATTACACTTTTTGGTGCGAAAATGTCCGGAGAGTTTATACGTGCCAGAGTACGCGCCCAAAAAAGGGTTTTCCCTGCATCTACTATTTGTACGATCTCCGGAGAGCTTTTTACAAAATTACCGCTATATTGGTGTCCAGGATATACGAAACTATTCCCCGTTACACAGCGCAACCAAAACTACTTTCCAGTACAGTTTCAGCCATCGGATTTTCCATTGGCATTTCTTTATTACCACCCAGATACCTCGTCATTTTCTATTATACATGGAAAGGTCCTTGCAATGCGTTGTCTTATGTGTGCAATTATTTACGTCAGATGGGAAATTGTACAAATCCGGGAGGATACGCAACAGGATCTTACAACCGGCGGGTATTTTGGCAATGTTTTCAGAACAGCCGAACTCACATGGCTTATCTATATGGATCCCTTTTCCTTTGTGGAGCTGGCAAAGGGCCCTTCTGGAATGTACTTCGCCGGTGCCAAAACCGGCATATACCGCGCTCAAACAGCACTTATTTCTTTTATTATACAAGAAATCATCCAAAAAATATGTCACCAATCCTGGGTTATTGTTCTTGGAATACGAAAGGGGCAGGACCTACGACGTTACCTGGACGCAGGGGTATGGCATCTTGTTGGAATCGATATGGACCAAACCGCGCTCGCGGAGCTTGTTTATCGAAAATTTTCGCATGCTACAACCCGACAGCACAAGCACGCTACCAACATTTACGTGTTGCATCAAGACCTCGCAGAGCCTGCGAAGGAAATCAGCGAAAAGGTACACCAAATTTACGGGTTTCCCAAGGAGGGAGCTTCTTCCATTGTTACCAACCTGTTTATTCACTATCTTATGCAAAACACGCAGCAGGTGGAAAACCTGGCCGTTCTGTGCCATATGCTTCTTCAGCCGGGGGGAATGGTGTGGTTTACCACCATGTTGGGAGAACAGGTCTTACAATTACTTCATGCAAATACAATACAGCTCAATGTACTATGGGAGGCACGTGCAAACGAGGTGGTCAAATTTGCTATTATACGTCTCTTTATGGAGGATATATTACAGGAAACTGGGCAAGAAATTGGAGTCCTGTTACCCTTCAGCAATGGCGACTTCTACAATGTATATCTTGTGTACACAGCGTTTTTATTTACAATATTTATACATCACGGCTTTTCCCTACTTCAAAAGCAGTCCTTTATGGACTGGATTCCAGAATTTCAAAACTTTACTACAAGTTTGTATACAATTCTTACAGAAGCCGATACAACTTGGACAAGCCTTTTTGGGTTTATTTGTCTGCGCAAAAATTAAATATTTTTTCATAAGAAGTACTACCCAGGTTTTAAAGAAATAG

Protein

MASLHILLARYQRCFIVQSLTNTTIVLAIRFQQIYFLLFKTVYVALVAQEIPTTISHSIRCIKKVHHANHCREKILPSENLYFKKQPLMFFMFSEPASLGCMVSLAIEQPIRIFILDSSVLVRLKNRTTFRVSVLWKIQLTIVYQLLGSEVSAKLAAFKTLLFVTPEQQTTKNMLSLLYPDVEYLYEIQIQYTGKPESLSAADVIQITNTVLSLISPNHLLLSAYHQAIVFIASHILSSEILLARIMCGKWGLIRLLPQVQSMSKADYMQFYPPVGYYVSDKADGITGIAVIQDTQIYVVADQLYSLRTTGIVPLIPTILDGVFMPAKKEFYGFAVIMYVGNLLSQQGFATRIVSLCKGIILLQAFIIQAEMYPFISLSSADPNVLLKNFASIFMQKTRPYSIVGIILLQPGNSYLYTNTFMWKPTWDITLHFLVRKCPESLYVPEYAPKKGFSLHLLFVRSPESFLQNYRYIGVQDIRNYSPLHSATKTTFQYSFSHRIFHWHFFITTQIPRHFLLYMERSLQCVVLCVQLFTSDGKLYKSGRIRNRILQPAGILAMFSEQPNSHGLSIWIPFPLWSWQRALLECTSPVPKPAYTALKQHLFLLLYKKSSKKYVTNPGLLFLEYERGRTYDVTWTQGYGILLESIWTKPRSRSLFIENFRMLQPDSTSTLPTFTCCIKTSQSLRRKSAKRYTKFTGFPRRELLPLLPTCLFTILCKTRSRWKTWPFCAICFFSRGEWCGLPPCWENRSYNYFMQIQYSSMYYGRHVQTRWSNLLLYVSLWRIYYRKLGKKLESCYPSAMATSTMYILCTQRFYLQYLYITAFPYFKSSPLWTGFQNFKTLLQVCIQFLQKPIQLGQAFLGLFVCAKIKYFFIRSTTQVLKKX

Function

Catalyzes the second reaction in the mRNA cap formation pathway.

Catalytic Activity

a 5'-triphospho-(purine-ribonucleotide)-[mRNA] + H2O = a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + H(+) + phosphate
a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + GTP + H(+) = a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + diphosphate
a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-homocysteine
a 5'-triphospho-(purine-ribonucleotide)-[mRNA] + H2O = a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + H(+) + phosphate
a 5'-diphospho-(purine-ribonucleotide)-[mRNA] + GTP + H(+) = a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + diphosphate
a 5'-(5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-methionine = a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] + S-adenosyl-L-homocysteine

Similarity

In the N-terminal section; belongs to the dsDNA virus mRNA guanylyltransferase family.
In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.

Keywords

Complete proteome   GTP-binding   Hydrolase   Methyltransferase   mRNA capping   mRNA processing   Nucleotide-binding   Nucleotidyltransferase   RNA-binding   S-adenosyl-L-methionine   Transferase   Reference proteome  

Feature

chain  Probable mRNA-capping enzyme

Uniprot

D4I5T8   A0A0A1DYG7   A9JM02   A0A0N7CR23   A0A0A1DXR2   A9JL69   + More
A0A1C6ZXW9   P0C993   P32094   P0C994   E0WM51   A0A2X0TKL6   Q65216   P0C995   A0A0C5AWD7   A0A2Z5DGW7   A0A0C5AWT8  

Pubmed

25406173    18198370    26618713    8382399    11831707    21470447    + More
8021596    25645905   

EMBL

FN557520    CBH29201.1    KM262845    AIY22450.1    AM712240    CAN10450.1    + More
KP055815    AKO62782.1    KM262844    AIY22292.1    AM712239    CAN10200.1    KM102979    KX354450    AJZ77049.1    AOO54458.1    AY261363    L07263    U18466    AY261366    FR682468    CBW46767.1    MG939583    MG939588    LS478113    AXZ95875.1    AXZ96158.1    SPS73524.1    X71982    AY261361    AY261360    KM111294    AJL34114.1    MH025916    MH025917    MH025918    MH025919    MH025920    AXB49329.1    AXB49503.1    AXB49675.1    AXB49846.1    AXB50019.1    KM111295    AJL34279.1   

Proteomes

UP000101090    UP000110401    UP000108903    UP000241813    UP000142390    UP000130745    + More
UP000117635    UP000266411    UP000000859    UP000000624    UP000000858    UP000141072    UP000000860    UP000000861    UP000105860    UP000101566   

PRIDE

P0C995   

Pfam

PF01331   mRNA_cap_enzyme
PF03291   Pox_MCEL

Interpro

IPR001339   mRNA_cap_enzyme
IPR004971   mRNA_G-N7_MeTrfase_dom
IPR033469   CYTH-like_dom_sf
IPR029063   SAM-dependent_MTases

SUPFAM

SSF55154   SSF55154
SSF53335   SSF53335

ProteinModelPortal

D4I5T8   A0A0A1DYG7   A9JM02   A0A0N7CR23   A0A0A1DXR2   A9JL69   + More
A0A1C6ZXW9   P0C993   P32094   P0C994   E0WM51   A0A2X0TKL6   Q65216   P0C995   A0A0C5AWD7   A0A2Z5DGW7   A0A0C5AWT8  

KEGG

vg:22220330    

GO

GO:0006370  P:7-methylguanosine mRNA capping
GO:0004484  F:mRNA guanylyltransferase activity
GO:0003723  F:RNA binding
GO:0004651  F:polynucleotide 5'-phosphatase activity
GO:0005525  F:GTP binding
GO:0004482  F:mRNA (guanine-N7-)-methyltransferase activity

From MSLVP

Others-Single

Length:

883

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

27.31951

Exp number, first 60 AAs:

3.30921

Total prob of N-in:

0.14173

Length:

882

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

27.40503

Exp number, first 60 AAs:

3.30942

Total prob of N-in:

0.14174

outside

1  -  883

outside

1  -  882

 

 

Pi

0.0289763

Theta

0.0398217

Tajima's D

-0.9278415

CLR

0

Interpretation

No evidence of Selection