Strain

Ukraine_Kyiv_2014 (Region: Ukraine;  Strain: ASFV/Kyiv/2016/131;  Date: 2014)

Gene

EP1242L

Description

Annotated in NCBI,  ASFV_Kyiv_2016_131_00079

Location

MN194591.1(-):69748-71430

GenBank Accession

QED21615.1

Full name

DNA-directed RNA polymerase subunit 2       + More
DNA-directed RNA polymerase subunit beta      

CDS

ATGCGGGGCAGCAACCGCTGCCACACTCATCACCTATCAAAAACCGCAAAAAAAGAAATAGGAGAAGACCCTAATGAACCCGGCGGCTACTTTATAGCCCGAGGCGGGGAATGGGTCGTAGACCTCTTAGAAAACATCCGCTTTAACACGCTTCACATCCATTACCATACCATGCAACAAGGGAATAATGAAATCATTCGCGGCGAGTTTATCTCCCAACCGGGAGGTGCCTTCGAAAATAGCTCTCAAATTATTATACGCTACATGACCACCGGCGCCATTACCATTGAAATCAATAGCACCAAGTTCTCAAAACTTCGCATACCTTGGTATCTCATCTTTCGCATGTTTGGAATGACAGGCGATGACAGCATCATTGAGCAAGTGGTATTTGATCTAGAATCCAACTCCCTTGTGAATACCTTCATGATAGAAATTCTGGAAAAATCCATTCATGTTTTAGACCCCATATTTCAACCGGTCCAACACGAGCTGAACCGAGAAAAAATTATCCAGTTTTTATCCGAGAAGGTTTCCAAATTTGTCTCAAACCCATCTGCTTACAAAAGCGATGAAAATGCGGTACAATACTTAAATGAAAGACAACTTACTATTTTAGACAAAATATTGCTGCCACACATGGGCCAAACCGCAGATACACGTGTACGAAAGCTGCGCTTTTTAGGCCTGCTTATTCATAAAATACTTCTTGTCATTATGAATGTGTTTCCACCCACGGACCGTGATAGCTACCGCACCAAGCGGGTGCACGGCTCAGGGGTCTCACTCGCCAAGGCCTTTAAGGCTATTTTTAATACTTCCGTGATAGCACCCATCATCAATGGGTTCAAAGAACTTCTTAAACAAACAGCCTTTGAGGAGCTTACTCAGCGAAACATTATCGAGGCATTTTCTGCCGCTTTATCAAAAAATACCGCCTCCGATCTTAATCGCAGTATGGAACAGTCTATCATTTCAGGAAATAAAACCATCATGGTGCGACAGCGCCCCATTGTAAACAGAGTATCTACTCAAAGTTTGGAAAGAAAAAATTTATTAAATACCATTTCAGCATTGCGTACGGTAAATACTCATAACACCACCAACGCATCCAAACAAACAGAAAGGGCTGACATGATGCGTCGCGTGCACGCATCCTATCCTGGATACATCTGCGTAGCCCAGTCAGCCGATACTGGTGAAAAAGTAGGCATGTCTAAACAGTTAGCCATCACTGCCAATGTCTGCACCGCCGGAGAAGTACTTTCGTTAAAACAACGCCTTCTATCAGATCCGGCCATTCAACAGTTAGCCGATGTTTCCAACAAGGACATTGTACGAAAGGGCCTAGCGCGCGTATTTATCAACGGAGAATGGATCGGCTGCTGCACCAATGCCTTTGAGCTTGCTCAGCGGTATAGAATGCTTCGCCGTGAGGGCAAGGTTGTGCATCCCCATACCACCATCTACTGGGATAGCATGGTAGACGAAGTAGAATTTTGGTTGGACGTCGGTAGGCTGACCCGCCCCCTACTCATTGTAGATAATAATATTGAAAAATACAATCAAGCCTGCTACAAGGCCGCAGAGGCCAGAAAAAAGGCGATAAAGACTGGGAAAAACATAAAATCCCTTTCATTCAAAATACGCGATTTACGCCTCAAATGGCAAAAGACATTTTAG

Protein

MRGSNRCHTHHLSKTAKKEIGEDPNEPGGYFIARGGEWVVDLLENIRFNTLHIHYHTMQQGNNEIIRGEFISQPGGAFENSSQIIIRYMTTGAITIEINSTKFSKLRIPWYLIFRMFGMTGDDSIIEQVVFDLESNSLVNTFMIEILEKSIHVLDPIFQPVQHELNREKIIQFLSEKVSKFVSNPSAYKSDENAVQYLNERQLTILDKILLPHMGQTADTRVRKLRFLGLLIHKILLVIMNVFPPTDRDSYRTKRVHGSGVSLAKAFKAIFNTSVIAPIINGFKELLKQTAFEELTQRNIIEAFSAALSKNTASDLNRSMEQSIISGNKTIMVRQRPIVNRVSTQSLERKNLLNTISALRTVNTHNTTNASKQTERADMMRRVHASYPGYICVAQSADTGEKVGMSKQLAITANVCTAGEVLSLKQRLLSDPAIQQLADVSNKDIVRKGLARVFINGEWIGCCTNAFELAQRYRMLRREGKVVHPHTTIYWDSMVDEVEFWLDVGRLTRPLLIVDNNIEKYNQACYKAAEARKKAIKTGKNIKSLSFKIRDLRLKWQKTF

Function

Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit 2 is the second largest component of RNA polymerase. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. DNA-dependent RNA polymerase is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic Activity

a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)

Subunit

Component of the RNA polymerase that consists of at least 6 subunits.
Component of the RNA polymerase that consists of at least 6 subunits.

Miscellaneous

The binding of ribonucleoside triphosphate to the RNA polymerase transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol subunits (By similarity).
The binding of ribonucleoside triphosphate to the RNA polymerase transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol subunits (By similarity).

Similarity

Belongs to the RNA polymerase beta chain family.

Keywords

Complete proteome   DNA-directed RNA polymerase   Host cytoplasm   Metal-binding   Nucleotidyltransferase   Transcription   Transferase   Zinc   Zinc-finger   Reference proteome  

Feature

chain  DNA-directed RNA polymerase subunit 2

Uniprot

A0A5B8XA79   P0C8K5   P0C8K4   A9JL02   D4I5P1   A9JLR5   + More
A0A0A1E066   A0A1C6ZXW3   E0WMJ1   A0A2X0RU95   P42487   P0C8K3   A0A0C5AYW2   A0A0C5AZ99   P0C8K2   A0A2Z5DG98   A0A1X7QFP1   A0A142C0P0   A0A141ZIB2   A0A0H3TLG3   A0A1X6WEN1   A0A1X7BZ16   A0A1V0CPA2   A0A2H1EED6   A0A533URQ6   A0A1Q7G3R8   A0A1Q7NN02   A0A1Q7CVP9   A0A1Q7KYQ4   A0A533WU03   A0A533UGP2   A0A5E4LTF6   A0A533VNH9   I3D5L8   A0A2H1FI76   A0A2G9P0F4   A0A128A178   K0BCP6   A0A2S2KQ11   A0A0D5C5A9   A0A2H9RPM0   A0A2H9MVU2   A0A2G9MW10   F9CUN4   A0A370LL05   A0A2H9P1U1   F3KKS9   A0A388QM46   A0A4P5YBW3   S2E7I8   A0A521U435   A0A2G4JFW2   A0A522Z648   V6ARL3   A0A522XKZ8   A0A3A4V2C0   A0RXX0   A0A0P8Y392   A0A5E4JXE3  

EC Number

2.7.7.6
2.7.7.6

Pubmed

18198370    25406173    26618713    21470447    8506138    11831707    + More
25645905    26903952    25878099    27801826    31110364    27843720    22461555    23209211    26528837    21914867    21364937    22461554    29079621    28644444    17114289   

EMBL

MN194591    QED21615.1    AY261366    AY261363    AM712239    CAN10153.1    + More
FN557520    CBH29154.1    AM712240    CAN10401.1    KM262844    KM262845    KP055815    AIY22244.1    AIY22403.1    AKO62735.1    KM102979    KX354450    AJZ77042.1    AOO54384.1    FR682468    CBW46719.1    MG939583    MG939587    MG939588    LS478113    AXZ95829.1    AXZ96017.1    AXZ96112.1    SPS73476.1    Z21490    U18466    AY261361    KM111294    AJL34067.1    KM111295    AJL34232.1    AY261360    MH025916    MH025917    MH025918    MH025919    MH025920    AXB49282.1    AXB49456.1    AXB49628.1    AXB49799.1    AXB49972.1    CZDJ02000003    SMH63388.1    KU702949    KU702951    KU556803    AMN83870.1    AMN84468.1    AMP44390.1    KU702948    KU702952    AMN82980.1    AMN84950.1    KJ614390    KU702950    AIB51752.1    AMN83967.1    LT706986    SIP85761.1    LT839607    SME64703.1    KX552040    ARA72182.1    FRFC01000001    SHO42515.1    VBPS01000081    TLX83474.1    MNFB01000064    OLC33654.1    MNGX01000149    OLD28660.1    MNED01000043    OLB90768.1    MNFZ01000037    OLC93301.1    VBPD01000024    TLY08475.1    VBPZ01000130    TLX69971.1    CABMJN010000025    VVC05246.1    VBPL01000040    TLX94524.1    AEXL02000008    EIJ67011.1    LT841358    SMH72468.1    PFCM01000353    PIN96807.1    LN890280    CUR51077.1    CP003843    AFS82146.1    BGKI01000002    GBH33780.1    CP011070    AJW71582.1    PFRJ01000106    PJC50142.1    PFGA01000294    PIW35501.1    PCWH01000304    PIN83041.1    AFPU01000001    EGP93080.1    QQVT01000007    QQVN01000004    QQVH01000002    QQVA01000001    RDJ31631.1    RDJ33574.1    RDJ38103.1    RDJ39128.1    PFLV01000304    PIY88190.1    AEGP01000040    EGG42088.1    BGOU01000002    GBL41117.1    BJHR01000001    GDY15239.1    AHJG01000013    EPA06703.1    SCUP01000060    TAK26642.1    NSIR01000002    PHY09505.1    SCVP01000042    TBR26002.1    CBTY010000006    CDI05204.1    SCVO01000126    TBR06709.1    QZIM01000013    RJQ21012.1    DP000238    ABK78187.1    JPUE01000079    KPU81155.1    CABMGU010000092    VVB81924.1   

Proteomes

UP000321214    UP000000858    UP000000859    UP000130745    UP000101090    UP000108903    + More
UP000110401    UP000142390    UP000241813    UP000117635    UP000266411    UP000141072    UP000000624    UP000000860    UP000105860    UP000101566    UP000000861    UP000244556    UP000244783    UP000244814    UP000244713    UP000244774    UP000244303    UP000244833    UP000201921    UP000204182    UP000232412    UP000320102    UP000186032    UP000186093    UP000186326    UP000186908    UP000315795    UP000318258    UP000319260    UP000003423    UP000230607    UP000228977    UP000196239    UP000006100    UP000245829    UP000032408    UP000229743    UP000230041    UP000229744    UP000004440    UP000254486    UP000255032    UP000255488    UP000255550    UP000229023    UP000004348    UP000266471    UP000014065    UP000315969    UP000242812    UP000320067    UP000018159    UP000318923    UP000266049    UP000000758    UP000054200   

PRIDE

P0C8K3   

Pfam

PF04566   RNA_pol_Rpb2_4
PF04565   RNA_pol_Rpb2_3
PF04561   RNA_pol_Rpb2_2
PF00562   RNA_pol_Rpb2_6
PF04560   RNA_pol_Rpb2_7
PF04563   RNA_pol_Rpb2_1
PF04567   RNA_pol_Rpb2_5
PF14528   LAGLIDADG_3

Interpro

IPR015712   DNA-dir_RNA_pol_su2
IPR007642   RNA_pol_Rpb2_2
IPR007645   RNA_pol_Rpb2_3
IPR037034   RNA_pol_Rpb2_2_sf
IPR007646   RNA_pol_Rpb2_4
IPR037033   DNA-dir_RNAP_su2_hyb_sf
IPR007120   DNA-dir_RNAP_su2_dom
IPR007641   RNA_pol_Rpb2_7
IPR007121   RNA_pol_bsu_CS
IPR007644   RNA_pol_bsu_protrusion
IPR014724   RNA_pol_RPB2_OB-fold
IPR007647   RNA_pol_Rpb2_5
IPR004042   Intein_endonuc
IPR006142   INTEIN
IPR027434   Homing_endonucl
IPR036844   Hint_dom_sf
IPR004860   LAGLIDADG_2
IPR019969   RNAP_B

SUPFAM

SSF55608   SSF55608
SSF51294   SSF51294

Gene 3D

3.90.1110.10     2.40.270.10     2.40.50.150     3.10.28.10     3.90.1110.10     2.40.270.10     2.40.50.150     3.10.28.10    

CDD

cd00653  RNA_pol_B_RPB2
cd00653  RNA_pol_B_RPB2

ProteinModelPortal

A0A5B8XA79   P0C8K5   P0C8K4   A9JL02   D4I5P1   A9JLR5   + More
A0A0A1E066   A0A1C6ZXW3   E0WMJ1   A0A2X0RU95   P42487   P0C8K3   A0A0C5AYW2   A0A0C5AZ99   P0C8K2   A0A2Z5DG98   A0A1X7QFP1   A0A142C0P0   A0A141ZIB2   A0A0H3TLG3   A0A1X6WEN1   A0A1X7BZ16   A0A1V0CPA2   A0A2H1EED6   A0A533URQ6   A0A1Q7G3R8   A0A1Q7NN02   A0A1Q7CVP9   A0A1Q7KYQ4   A0A533WU03   A0A533UGP2   A0A5E4LTF6   A0A533VNH9   I3D5L8   A0A2H1FI76   A0A2G9P0F4   A0A128A178   K0BCP6   A0A2S2KQ11   A0A0D5C5A9   A0A2H9RPM0   A0A2H9MVU2   A0A2G9MW10   F9CUN4   A0A370LL05   A0A2H9P1U1   F3KKS9   A0A388QM46   A0A4P5YBW3   S2E7I8   A0A521U435   A0A2G4JFW2   A0A522Z648   V6ARL3   A0A522XKZ8   A0A3A4V2C0   A0RXX0   A0A0P8Y392   A0A5E4JXE3  

PANTHER

PTHR20856   PTHR20856  

PDB

6GML     E-value=7.92573e-32,     Score=344