Strain

Lithuania_LT14_2014 (Region: Lithuania; Strain: ASFV/LT14/1490; Date: 2014)

Gene

S273R

Description

Annotated in NCBI, NA

Location

MK628478.1(+):146707-147528

GenBank Accession

QEY87917.1

Full name

Cysteine protease S273R

CDS

ATGTCTATATTAGAAAAAATTACGTCAAGTCCCTCTGAATGCGCAGAGCATCTTACAAACAAAGATAGCTGTTTAAGTAAAAAAATACAAAAAGAGCTCACCTCTTTTTTGGAAAAAAAAGAGACACTCGGTTGCGATTCGGAGTCCTGCGTAATTACCCACCCCGCCGTGAAGGCCTATGCGCAACAAAAGGGACTGGACCTCTCCAAAGAACTGGAGACTCGGTTTAAAGCGCCAGGACCCAGAAACAACACGGGTCTTCTTACAAACTTCAATATTGATGAAACGCTGCAGAGGTGGGCCATAAAATACACCAAGTTTTTCAACTGTCCTTTTTCCATGATGGACTTTGAGAGGGTCCATTATAAATTTAATCAAGTGGATATGGTAAAGGTATATAAGGGAGAAGAGCTACAATATGTAGAAGGCAAAGTGGTCAAGCGTCCTTGTAACACCTTCGGATGCGTTTTAAACACGGACTTTTCAACGGGCACTGGAAAACACTGGGTAGCCATCTTTGTGGATATGCGGGGCGACTGCTGGAGCATCGAATATTTTAATTCGACGGGAAATTCTCCTCCAGGTCCCGTTATTCGTTGGATGGAACGGGTCAAACAGCAGCTATTAAAAATACACCACACCGTGAAAACGCTTGCAGTTACCAACATTCGTCACCAACGGTCGCAGACCGAGTGCGGCCCCTACAGCCTGTTTTACATCAGGGCACGCCTCGACAACGTGTCATACGCCCATTTTATATCCGCTAGGATTACCGACGAAGACATGTATAAGTTTAGAACCCATCTGTTTCGCATCGCATAA

Protein

MSILEKITSSPSECAEHLTNKDSCLSKKIQKELTSFLEKKETLGCDSESCVITHPAVKAYAQQKGLDLSKELETRFKAPGPRNNTGLLTNFNIDETLQRWAIKYTKFFNCPFSMMDFERVHYKFNQVDMVKVYKGEELQYVEGKVVKRPCNTFGCVLNTDFSTGTGKHWVAIFVDMRGDCWSIEYFNSTGNSPPGPVIRWMERVKQQLLKIHHTVKTLAVTNIRHQRSQTECGPYSLFYIRARLDNVSYAHFISARITDEDMYKFRTHLFRIA

Function

Protease involved in the processing of both viral polyproteins pp220 and pp62.

Similarity

Belongs to the peptidase C63 family.

Keywords

Complete proteome Host cytoplasm Hydrolase Late protein Protease Reference proteome Thiol protease Virion

Feature

chain Cysteine protease S273R

Uniprot

D4I5U8 A9JL89 A9JM21 A0A0A1DYH8 Q00946 E0WM61 + More
A0A2X0TKM5 P0C9B7 P0C9B8 P0C9B9 A0A0C5AWE3 Q65228 A0A2Z5DG24 A0A0C5AWU8 A0A2Z6QGL0

Pubmed

18198370 25406173 26618713 1316688 11831707 11031264 + More
12634404 12719549 21470447 25645905 8021596

EMBL

FN557520 CBH29211.1 AM712239 CAN10211.1 AM712240 CAN10460.1 + More
KM262844 KM262845 KM102979 KP055815 KX354450 AIY22302.1 AIY22460.1 AJZ77109.1 AKO62792.1 AOO54471.1 M88699 U18466 FR682468 CBW46777.1 MG939583 MG939588 LS478113 AXZ95884.1 AXZ96167.1 SPS73534.1 AY261363 AY261366 AY261360 KM111294 AJL34124.1 X71982 AY261361 MH025916 MH025917 MH025918 MH025919 MH025920 AXB49340.1 AXB49514.1 AXB49686.1 AXB49857.1 AXB50030.1 KM111295 AJL34289.1 BEXD01000665 GBB89267.1

Proteomes

UP000101090 UP000130745 UP000108903 UP000110401 UP000117635 UP000142390 + More
UP000241813 UP000266411 UP000000624 UP000141072 UP000000859 UP000000858 UP000000861 UP000105860 UP000000860 UP000101566 UP000247702

PRIDE

P0C9B9

Pfam

PF02902 Peptidase_C48

Interpro

IPR016510   Protease_ASVF_polyprot-process
IPR003653   Peptidase_C48_C
IPR038765   Papain_like_cys_pep_sf

SUPFAM

SSF54001 SSF54001

ProteinModelPortal

D4I5U8 A9JL89 A9JM21 A0A0A1DYH8 Q00946 E0WM61 + More
A0A2X0TKM5 P0C9B7 P0C9B8 P0C9B9 A0A0C5AWE3 Q65228 A0A2Z5DG24 A0A0C5AWU8 A0A2Z6QGL0

PDB

3IJD E-value=4.54148, Score=67

Ontologies

KEGG

vg:22220340

GO

GO:0004197  F:cysteine-type endopeptidase activity
GO:0019082  P:viral protein processing
GO:0019012  C:virion
GO:0030430  C:host cell cytoplasm

Subcellular Location

From MSLVP

Cytoplasm

From Uniprot

Host cytoplasm
Virion

Topology

Length:

273

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00147

Exp number, first 60 AAs:

0

Total prob of N-in:

0.03352

outside

1 - 273

Population Genetic Test Statistics

Pi

0.0289763

Theta

0.0364084

Tajima's D

-0.7661231

CLR

0.067445

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62792.1
China_AnhuiXCGQ_2018	Protein	AYW34082.1
China_ASFV-SY18_2018	Protein	AKO62792.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95884.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10211.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10211.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10211.1
Poland_Pol17_03029_C201_2016-2017	Protein	CAN10211.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96167.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10211.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10211.1
Uganda_R8_2015	Protein	AXB49340.1
Uganda_R7_2015	Protein	AXB49514.1
Uganda_R25_2015	Protein	AXB49686.1
Uganda_N10_2015	Protein	AXB49857.1
Uganda_R35_2015	Protein	AXB50030.1
Estonia_2014	Protein	SPS73534.1
Italy_26544_OG10_2010	Protein	AJZ77109.1
Italy_47_Ss_2008_2008	Protein	AOO54471.1
Portugal_OURT_88.3_1988	Protein	CAN10460.1
Spain_E75_1975	Protein	CBH29211.1
Russia_Georgia_2007	Protein	CBW46777.1
Kenya_Tk1_2005	Protein	AJL34124.1
Kenya_Bus_2006	Protein	AJL34289.1
Russia_Odintsovo_2014	Protein	CAN10211.1
Portugal_L60_1960	Protein	AIY22302.1
Portugal_NHV_1968	Protein	AIY22460.1
Russia_Kashino_2013	Protein	CAN10211.1
Benin_1997	Protein Protein	CAN10211.1 CAN10211.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10211.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10211.1
Malawi_Lil_1983	Protein	CAN10211.1
Kenya_1950	Protein	CAN10211.1
Namibia_Warthog_1980	Protein	CAN10211.1
South_Africa_Warmbaths_1987	Protein	CAN10211.1
Malawi_Tengani_1962	Protein	CAN10211.1
China_HLJ_2018	Protein	QBH90599.1
China_LN_2018	Protein	QBH90784.1
China_wbBS01_2018	Protein	QDL88142.1
Ukraine_Kyiv_2014	Protein	QED21715.1
Belgium_Etalle_wb_2018	Protein	QED90554.1
Lithuania_LT14_2014	Protein	QEY87917.1
South_Africa_1985	Protein	QGM12931.1
South_Africa_2_2008	Protein	QGM12857.1
Zambia_LIV_1983	Protein	QGM12749.1

Sequence