Strain

China_AnhuiXCGQ_2018 (Region: China;  Strain: China/2018/AnhuiXCGQ;  Date: 2018)

Gene

O174L

Description

Annotated in NCBI,  product is similar to DNA polymerase beta, Swiss-Prot Accession Number P06746 and terminal deoxynucleotidyl transferase, encoded by GenBank Accession Number U07803

Location

MK128995.1(-):128251-128775

GenBank Accession

AYW34068.1

Full name

Repair DNA polymerase X      

CDS

ATGTTAACGCTTATTCAAGGAAAAAAAATTGTAAATGACTTACGCTCCCGACTTGCGTTTGAATATAATGGACAACTTATAAAAATTTTATCAAAAAACATCGTTGCTGTTGGTAGCTTAAGACGCGAAGAGAAAATGCTTAATGACGTGGATCTTCTTATTATTGTTCCAGAAAAAAAACTTTTAAAACACGTCCTGTCCAACATTCGCATAAAGGATCTTTTTTTTTCTGTAAAAGTCTGCGGAGAACGAAAGTGTGTACTTTTTATTGAATGGAAAAAAAACACATATCAACTTGATCTTTTTACGGCCTTAGCCGAGGAAAAGTCATACGCAGTACTTCATTTTACGGGTCCCGTTTCTTATTTAATAAGAATTCGAGCTGCGTTAAAAAAAAAGAATTATAAGCTAAATCAGTATGGATTATTTAAAAATCAAACTTTAGTACCTCTAAAAATCACTACTGAAAAAGAACTTATTAAAGAATTAGGATTTACGTATCGCATACCTAAGAAACGTTTATAA

Protein

MLTLIQGKKIVNDLRSRLAFEYNGQLIKILSKNIVAVGSLRREEKMLNDVDLLIIVPEKKLLKHVLSNIRIKDLFFSVKVCGERKCVLFIEWKKNTYQLDLFTALAEEKSYAVLHFTGPVSYLIRIRAALKKKNYKLNQYGLFKNQTLVPLKITTEKELIKELGFTYRIPKKRL

Function

Error-prone polymerase lacking a proofreading 3'-5' exonuclease which plays a role in viral DNA repair. Specifically binds intermediates in the single-nucleotide base-excision repair process. Also catalyzes DNA polymerization with low nucleotide-insertion fidelity. Together with the viral DNA ligase, fills the single nucleotide gaps generated by the AP endonuclease (By similarity).
Error-prone polymerase lacking a proofreading 3'-5' exonuclease which plays a role in viral DNA repair. Specifically binds intermediates in the single-nucleotide base-excision repair process. Also catalyzes DNA polymerization with low nucleotide-insertion fidelity. Together with the viral DNA ligase, fills the single nucleotide gaps generated by the AP endonuclease.

Catalytic Activity

a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)

Cofactor

Mg(2+)

Miscellaneous

Consistent with its intracellular location, ASFV encodes its own replicative DNA polymerase and three base excision repair enzymes: a class II AP endonuclease, the repair polymerase Pol X, and an ATP-dependent DNA ligase.

Similarity

Belongs to the DNA polymerase type-X family.

Keywords

Complete proteome   DNA damage   DNA repair   DNA-binding   DNA-directed DNA polymerase   Magnesium   Metal-binding   Nucleotidyltransferase   Transferase   3D-structure   Disulfide bond   Reference proteome  

Feature

chain  Repair DNA polymerase X

Uniprot

E0WM47   A0A2X0TCB0   P0C985   P0C983   A9JL63   D4I5T4   + More
A9JLZ4   A0A0A1E3N6   P42494   P0C984   P0C986   A0A0C5AZI4   A0A2Z5DF27   A0A0C5B057  

Pubmed

21470447    18198370    25406173    26618713    11831707    12595253    + More
17144676    17941646    17765921    11685238    11685239    25645905   

EMBL

FR682468    CBW46763.1    MG939583    MG939588    LS478113    AXZ95871.1    + More
AXZ96154.1    SPS73520.1    AY261366    AY261363    AM712239    CAN10196.1    FN557520    CBH29197.1    AM712240    CAN10446.1    KM262844    KM262845    KM102979    KP055815    KX354450    AIY22288.1    AIY22446.1    AJZ77144.1    AKO62778.1    AOO54447.1    U18466    AY261361    AY261360    KM111294    AJL34110.1    MH025916    MH025917    MH025918    MH025919    MH025920    AXB49325.1    AXB49499.1    AXB49671.1    AXB49842.1    AXB50015.1    KM111295    AJL34275.1   

Proteomes

UP000141072    UP000000858    UP000000859    UP000130745    UP000101090    UP000108903    + More
UP000110401    UP000117635    UP000142390    UP000241813    UP000266411    UP000000624    UP000000860    UP000000861    UP000105860    UP000101566   

Pfam

PF14791   DNA_pol_B_thumb

Interpro

IPR019843   DNA_pol-X_BS
IPR029398   PolB_thumb
IPR037160   DNA_Pol_thumb_sf

Gene 3D

3.30.210.10    

ProteinModelPortal

E0WM47   A0A2X0TCB0   P0C985   P0C983   A9JL63   D4I5T4   + More
A9JLZ4   A0A0A1E3N6   P42494   P0C984   P0C986   A0A0C5AZI4   A0A2Z5DF27   A0A0C5B057  

PDB

5HR9     E-value=7.13888e-86,     Score=804