Strain

Uganda_N10_2015 (Region: Uganda, Tororo district; Strain: N10; Date: 2015)

Gene

pK196R

Description

Annotated in NCBI, non-essential

Location

MH025919.1(+):62757-63314

GenBank Accession

AXB49796.1

Full name

Thymidine kinase

CDS

ATGAGTCTAATCAGAAAGCTTAAGCCTGGAACAATTAGCCTTGTGCTGGGACCTATGTTTGCTGGCAAAACGACGTTTCTTATTCATTGCATTAACATGCTCGAACGTTTAGAAAAAAAAGTAGTCTTCATAAAATCTACAAAAAACACCCGAGACAAAACGATTCAAACACACTCCGGTATACAGCTACGACCCAATCAATGTAAAATTATAGAAAGCACACAGCTATCTGATGTGGGATCTCTAACCGATACCCATGCAGTTGTGATAGATGAAGCGCATTTTTTTGACGATTTAATAAGATGCCGCACCTGGGCAGATGAAAAAAAAATTATTATTCTTGCGGGACTCAATGCTTCCTTCGAGCAGAAAATGTTTCAGCCCATCGTTCGTATTTTTCCTTACTGCAACTGGGTTAAGTATATTGGTCGCACCTGTATGAAATGTAACCGACATAATGCATGCTTTAATGTGCGTAAGAACGCGGACAAGTCGCTTATCCTTGCGGGAGGGAGTGAACTATACGTAACATGCTGTAATAACTGTCTAAAAAAATAA

Protein

MSLIRKLKPGTISLVLGPMFAGKTTFLIHCINMLERLEKKVVFIKSTKNTRDKTIQTHSGIQLRPNQCKIIESTQLSDVGSLTDTHAVVIDEAHFFDDLIRCRTWADEKKIIILAGLNASFEQKMFQPIVRIFPYCNWVKYIGRTCMKCNRHNACFNVRKNADKSLILAGGSELYVTCCNNCLKK

Function

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine (By similarity).
Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine.

Catalytic Activity

ATP + thymidine = ADP + dTMP + H(+)

Similarity

Belongs to the thymidine kinase family.

Keywords

ATP-binding Complete proteome DNA synthesis Kinase Metal-binding Nucleotide-binding Transferase Virulence Zinc Reference proteome

Feature

chain Thymidine kinase

Uniprot

A0A1W5LD83 A0A0C5AWN5 Q336D8 P0C8I3 A0A0C5AWA4 P0C8I5 + More
E0WMI8 Q336E6 Q336E4 A9JKZ6 D4I5N8 A9JLQ8 Q336F0 P18555 Q336E3 Q336E5 I6LA85 Q336E2 P0C8I6 I6LA84 P0C8I4 A0A0P0C6T6 I3UKP3 I3UJZ0 E4WM64 M4QQK4

EC Number

2.7.1.21

Pubmed

29143187 25645905 21470447 18198370 25406173 26618713 + More
2389555 1987368 11831707 9811782 25787287 21289127

EMBL

KU199302 ANC68039.1 KM111295 MH025916 MH025917 MH025918 + More
MH025919 MH025920 AJL34229.1 AXB49279.1 AXB49453.1 AXB49625.1 AXB49796.1 AXB49969.1 AF504904 AF504906 AF504907 AAQ07930.1 AAQ07932.1 AAQ07933.1 AY261360 KM111294 AJL34064.1 AY261363 FR682468 CBW46716.1 AF504897 MG939583 MG939587 MG939588 LS478113 AAQ07941.1 AXZ95826.1 AXZ96014.1 AXZ96109.1 SPS73473.1 AF504899 AAQ07943.1 AM712239 CAN10149.1 FN557520 CBH29151.1 AM712240 CAN10398.1 AF504890 AF504891 AF504892 AF504893 AF504895 AF504896 AF504902 KM262844 KM262845 KM102979 KP055815 KX354450 AAQ07934.1 AAQ07935.1 AAQ07936.1 AAQ07937.1 AAQ07939.1 AAQ07940.1 AAQ07946.1 AIY22241.1 AIY22400.1 AJZ77133.1 AKO62732.1 AOO54381.1 Z21490 M63119 U18466 AF504900 AAQ07944.1 AF504898 AAQ07942.1 AF504894 AAQ07938.1 AF504901 AAQ07945.1 AY261366 AF504903 AAQ07929.1 AY261361 KP874736 ALI95575.1 HQ633060 AFK66058.1 HQ633059 AFK65805.1 FN600414 CBI70187.1 HQ632827 AGH31128.1

Proteomes

UP000101566 UP000000861 UP000105860 UP000000859 UP000141072 UP000130745 + More
UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 UP000241813 UP000266411 UP000000624 UP000000858 UP000000860 UP000203965 UP000247639 UP000247891 UP000202189 UP000201445

PRIDE

P0C8I5 P18555 P0C8I4

Pfam

PF00265 TK

Interpro

IPR027417   P-loop_NTPase
IPR001267   Thymidine_kinase
IPR020633   Thymidine_kinase_CS

SUPFAM

SSF52540 SSF52540

ProteinModelPortal

A0A1W5LD83 A0A0C5AWN5 Q336D8 P0C8I3 A0A0C5AWA4 P0C8I5 + More
E0WMI8 Q336E6 Q336E4 A9JKZ6 D4I5N8 A9JLQ8 Q336F0 P18555 Q336E3 Q336E5 I6LA85 Q336E2 P0C8I6 I6LA84 P0C8I4 A0A0P0C6T6 I3UKP3 I3UJZ0 E4WM64 M4QQK4

PANTHER

PTHR11441

PDB

4UXI E-value=3.21218e-23, Score=264

Ontologies

KEGG

vg:22220432 vg:26049605 vg:10021170 vg:15011258

GO

GO:0071897  P:DNA biosynthetic process
GO:0004797  F:thymidine kinase activity
GO:0005524  F:ATP binding
GO:0046872  F:metal ion binding
GO:0009405  P:pathogenesis
GO:0016021  C:integral component of membrane

Subcellular Location

From MSLVP

Single-Pass Membrane

Topology

Length:

185

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

7.89092999999999

Exp number, first 60 AAs:

7.84905

Total prob of N-in:

0.26395

outside

1 - 185

Population Genetic Test Statistics

Pi

0.0300719

Theta

0.0375462

Tajima's D

-0.7255164

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971
China_AnhuiXCGQ_2018
China_ASFV-SY18_2018
Poland_Pol16_20186_o7_2016-2017
Poland_Pol16_20538_o9_2016-2017
Poland_Pol16_20540_o10_2016-2017
Poland_Pol16_29413_o23_2016-2017
Poland_Pol17_03029_C201_2016-2017
Poland_Pol17_04461_C210_2016-2017
Poland_Pol17_05838_C220_2016-2017
Poland_ASFV_POL_Podlaskie_2015
Uganda_R8_2015	Protein	AXB49279.1
Uganda_R7_2015	Protein	AXB49453.1
Uganda_R25_2015	Protein	AXB49625.1
Uganda_N10_2015	Protein	AXB49796.1
Uganda_R35_2015	Protein	AXB49969.1
Estonia_2014
Italy_26544_OG10_2010
Italy_47_Ss_2008_2008
Portugal_OURT_88.3_1988
Spain_E75_1975
Russia_Georgia_2007
Kenya_Tk1_2005	Protein	AJL34064.1
Kenya_Bus_2006	Protein	AJL34229.1
Russia_Odintsovo_2014
Portugal_L60_1960
Portugal_NHV_1968
Russia_Kashino_2013
Benin_1997
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10149.1
South_Africa_MGR_Mkuzi_1979
Malawi_Lil_1983
Kenya_1950	Protein	CAN10149.1
Namibia_Warthog_1980
South_Africa_Warmbaths_1987
Malawi_Tengani_1962
China_HLJ_2018
China_LN_2018
China_wbBS01_2018
Ukraine_Kyiv_2014
Belgium_Etalle_wb_2018
Lithuania_LT14_2014
South_Africa_1985
South_Africa_2_2008
Zambia_LIV_1983

Sequence