Strain

Poland_Pol17_04461_C210_2016-2017 (Region: Poland;  Strain: Pol17_04461_C210;  Date: 2016-2017)

Gene

D250R

Description

Annotated in NCBI,  pBA71-D250R

Location

MG939588.1(+):137519-138271

GenBank Accession

AXZ96159.1

Full name

mRNA-decapping protein g5R      

Alternative Name

ASFV-DP
Diphosphoinositol polyphosphate phosphohydrolase

CDS

ATGGATACTGCCATGCAGCTTAAAACGTCTATTGGTTTAATTACATGTCGTATGAACACCCAAAATAACCAAATAGAAACTATTCTGGTTCAAAAACGTTACAGCCTTGCTTTTTCAGAATTTATTCATTGTCATTACTCTATAAATGCTAATCAAGGTCATCTGATTAAAATGTTTAATAACATGACAATTAATGAACGACTGCTTGTCAAAACACTGGATTTTGACCGCATGTGGTATCATATTTGGATTGAAACTCCAGTCTACGAACTATACCACAAAAAATACCAAAAATTTAGGAAAAATTGGCTTCTCCCGGATAATGGGAAAAAGCTTATTTCATTAATCAACCAAGCAAAGGGCTCAGGAACACTTCTATGGGAAATCCCTAAGGGTAAGCCGAAGGAAGACGAGTCGGACCTTACCTGTGCCATACGGGAGTTTGAAGAAGAAACCGGGATTACCCGCGAATATTACCAGATTCTCCCAGAGTTTAAAAAATCTATGTCATACTTTGACGGTAAAACAGAATATAAGCATATCTACTTCCTTGCAATGTTATGTAAGTCGTTGGAGGAACCCAATATGAATCTTTCTTTACAATACGAAAACCGAATTGCCGAAATTTCTAAAATTTCTTGGCAAAATATGGAGGCTGTACGTTTTATTAGCAAACGCCAGTCATTAAACCTGGAGCCTATCATCGGGCCTGCATTTAATTTTATTAAAAACTATTTACGATACAAGCACTAG

Protein

MDTAMQLKTSIGLITCRMNTQNNQIETILVQKRYSLAFSEFIHCHYSINANQGHLIKMFNNMTINERLLVKTLDFDRMWYHIWIETPVYELYHKKYQKFRKNWLLPDNGKKLISLINQAKGSGTLLWEIPKGKPKEDESDLTCAIREFEEETGITREYYQILPEFKKSMSYFDGKTEYKHIYFLAMLCKSLEEPNMNLSLQYENRIAEISKISWQNMEAVRFISKRQSLNLEPIIGPAFNFIKNYLRYKH

Function

Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells. May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis. In addition to the mRNA cap, g5R also efficiently hydrolyzes diphosphoinositol polyphosphates. Down-regulation of the level of PP-InsP5 (diphosphoinositol pentakisphosphate) may play a role in viral manipulation of the cellular secretory pathway, a step necessary for the formation of virions. Binds viral and cellular poly(A) mRNAs, thereby decreasing both types of mRNAs.
Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells (PubMed:19695654). May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis (PubMed:29021398). In addition to the mRNA cap, g5R also efficiently hydrolyzes diphosphoinositol polyphosphates. Down-regulation of the level of PP-InsP5 (diphosphoinositol pentakisphosphate) may play a role in viral manipulation of the cellular secretory pathway, a step necessary for the formation of virions (PubMed:11773415). Binds viral and cellular poly(A) mRNAs, thereby decreasing both types of mRNAs (PubMed:19695654, PubMed:29021398).

Catalytic Activity

Diphospho-myo-inositol polyphosphate + H(2)O = myo-inositol polyphosphate + phosphate.

Biophysicochemical Properties

1.2 uM for diphosphoinositol pentakisphosphate
0.67 mM for GTP
0.58 mM for p5A
0.92 mM for ATP
1.36 mM for p4A
1.70 mM for Gp4G
3.90 mM for Ap4A

Subunit

Interacts with host RPL23A.

Similarity

Belongs to the Nudix hydrolase family. DIPP subfamily.

Keywords

Complete proteome   Early protein   Eukaryotic host gene expression shutoff by virus   Host endoplasmic reticulum   Host gene expression shutoff by virus   Host-virus interaction   Hydrolase   Late protein   Magnesium   Manganese   Metal-binding   Multifunctional enzyme   RNA-binding   Reference proteome  

Feature

chain  mRNA-decapping protein g5R

Uniprot

E0WM52   A0A2X0THV3   P0C996   P32092   A9JL71   D4I5T9   + More
A9JM04   A0A0A1E3P0   A0A0C5AZI9   A0A0C5B063   P0C997   Q65217   P0C998   A0A1X6WGW5   A0A015IDB9   U9UC43   A0A1X7QGA2   A0A142BZK9   A0A0H3TMC6   A0A1X7C0B4   A0A2J7ZJS7   A0A2J7ZIW2   A0A2J7ZR37  

EC Number

3.1.3.-

Pubmed

21470447    8382399    11831707    11773415    19695654    29021398    + More
18198370    25406173    26618713    25645905    8021596    26903952    25878099    29294063   

EMBL

FR682468    CBW46768.1    MG939583    MG939588    LS478113    AXZ95876.1    + More
AXZ96159.1    SPS73525.1    AY261363    L07263    U18466    AM712239    CAN10201.1    FN557520    CBH29202.1    AM712240    CAN10451.1    KM262844    KM262845    KM102979    KP055815    KX354450    AIY22293.1    AIY22451.1    AJZ77118.1    AKO62783.1    AOO54459.1    KM111294    AJL34115.1    KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    AJL34280.1    AXB49330.1    AXB49504.1    AXB49676.1    AXB49847.1    AXB50020.1    AY261366    X71982    AY261361    AY261360    LT706986    SIP86037.1    JEMT01028332    EXX55117.1    KI279659    ESA17955.1    CZDJ02000001    SMH63232.1    KU702949    KU702951    KU556803    AMN83482.1    AMN84859.1    AMP44009.1    KJ614390    KU702948    KU702950    KU702952    AIB52140.1    AMN83366.1    AMN84350.1    AMN85336.1    LT839607    SME65110.1    PGGS01001341    PNH00512.1    PGGS01001611    PNH00203.1    PGGS01000606    PNH02727.1   

Proteomes

UP000141072    UP000000859    UP000000624    UP000130745    UP000101090    UP000108903    + More
UP000110401    UP000117635    UP000142390    UP000241813    UP000266411    UP000105860    UP000101566    UP000000858    UP000000860    UP000000861    UP000201921    UP000022910    UP000244556    UP000244783    UP000244814    UP000244303    UP000244713    UP000244774    UP000244833   

PRIDE

P0C996    P32092    P0C997    Q65217    P0C998   

Pfam

PF00293   NUDIX

Interpro

IPR020084   NUDIX_hydrolase_CS
IPR015797   NUDIX_hydrolase-like_dom_sf
IPR000086   NUDIX_hydrolase_dom

SUPFAM

SSF55811   SSF55811

ProteinModelPortal

E0WM52   A0A2X0THV3   P0C996   P32092   A9JL71   D4I5T9   + More
A9JM04   A0A0A1E3P0   A0A0C5AZI9   A0A0C5B063   P0C997   Q65217   P0C998   A0A1X6WGW5   A0A015IDB9   U9UC43   A0A1X7QGA2   A0A142BZK9   A0A0H3TMC6   A0A1X7C0B4   A0A2J7ZJS7   A0A2J7ZIW2   A0A2J7ZR37  

PDB

5KQ4     E-value=1.54421e-05,     Score=114