Strain

Poland_Pol16_29413_o23_2016-2017 (Region: Poland;  Strain: Pol16_29413_o23;  Date: 2016-2017)

Gene

AP endonuclease class II

Description

Annoated by sequence mapping,  VOCs Family: AP endonuclease class II

Location

MG939586.1(+):168213-169103

GenBank Accession

CAN10233.1

Full name

Probable AP endonuclease      

CDS

ATGTTTGGAGCTTTTGTAAGCCACCGTTTGTGGTCAGATAGTGGTTGTACGACCACCTGCATCACAAACAGCATTGCTAATTATGTAGCCTTCGGCGAACAAATTGGATTTCCCTTTAAATCAGCTCAGGTATTTATTGCCGGCCCTAGAAAGGCTGTGATAAATATTCAGGAAGATGATAAAGTTGAGCTTTTAAAGATGATTGTTAAGCACAATCTTTGGGTTGTTGCTCATGGAACCTACTTAGATGTGCCCTGGTCCCGTAAGAGTGCGTTTGTTACACATTTTATACAACAAGAACTACTTATATGCAAGGAAGTCGGTATTAAAGGGTTAGTTTTACACCTAGGCGCTGTGGAGCCTGAACTTATTATGGAAGGACTAAAAAAAATTAAGCCGGTTGAGGGGGTTGTCATTTACCTGGAAACCCCGCATAACAAACATCATACATATAAATACAGTACAATTGAGCAGATCAAAGAATTGTTTTTACGGATACGAAATACCAGGTTGAAACAGATTGGTTTATGCATTGATACGGCTCACATCTGGTCTTCCGGTGTCAACATCTCCAGCTATAATGACGCGGGGCAATGGCTGCGCTCGCTGGAAAACATTCATTCCGTGATCCCACCAAGCCACATTATGTTCCACCTAAATGATGCCGCCACAGAATGCGGAAGCGGTATAGACCGACATGCAAGTCTTTTTGAAGGAATGATTTGGAAATCATATAGCCATAAAATAAAGCAAAGCGGTTTATATTGTTTTGTTGAATACGTTACGCGACACCAGTGTCCGGCTATATTGGAGAGAAACCTCGGGTCTTCCATGCAATTACAAACCGCTTTAACCGCAGAATTTACTACATTAAAATCGTTATTAAAATAA

Protein

MFGAFVSHRLWSDSGCTTTCITNSIANYVAFGEQIGFPFKSAQVFIAGPRKAVINIQEDDKVELLKMIVKHNLWVVAHGTYLDVPWSRKSAFVTHFIQQELLICKEVGIKGLVLHLGAVEPELIMEGLKKIKPVEGVVIYLETPHNKHHTYKYSTIEQIKELFLRIRNTRLKQIGLCIDTAHIWSSGVNISSYNDAGQWLRSLENIHSVIPPSHIMFHLNDAATECGSGIDRHASLFEGMIWKSYSHKIKQSGLYCFVEYVTRHQCPAILERNLGSSMQLQTALTAEFTTLKSLLKX

Function

Endonuclease that plays a role in DNA repair. Cleaves phosphodiester bonds on the 5' side of apurinic or apyrimidinic sites (AP sites). In addition to endonuclease activity, the ASFV enzyme has a proofreading 3'-5' exonuclease activity that is considerably more efficient in the elimination of a mismatch than in that of a correctly paired base. Displays 3'-phosphatase and 3'-repair diesterase activities. The single nucleotide gaps generated by the AP endonuclease are filled by the viral AP endonuclease and DNA ligase (By similarity).

Cofactor

Zn(2+)

Miscellaneous

Consistent with its intracellular location, ASFV encodes its own replicative DNA polymerase and three base excision repair enzymes: a class II AP endonuclease, the repair polymerase Pol X, and an ATP-dependent DNA ligase.
During infection, the protein is expressed at early times and accumulates at later times.

Similarity

Belongs to the AP endonuclease 2 family.

Keywords

Complete proteome   DNA damage   DNA repair   Early protein   Endonuclease   Exonuclease   Host cytoplasm   Host nucleus   Hydrolase   Metal-binding   Nuclease   Zinc   Reference proteome  

Feature

chain  Probable AP endonuclease

Uniprot

P0C9C6   A9JLC6   D4I5F7   A9JM65   A0A0A1DYA6   A0A1C6ZY19   + More
P0C9C7   Q65202   E0WM83   A0A2X0RUG5   Q65246   A0A0C5B2A8   P0C9C8   A0A0C5B2R9  

Pubmed

18198370    25406173    26618713    11831707    16641276    16503634    + More
21470447    8021596    25645905   

EMBL

AY261363    AM712239    CAN10233.1    FN557520    CBH29233.1    AM712240    + More
CAN10482.1    KM262844    KM262845    KP055815    AIY22324.1    AIY22482.1    AKO62814.1    KM102979    KX354450    AJZ77105.1    AOO54504.1    AY261366    U18466    FR682468    CBW46799.1    MG939583    MG939588    LS478113    AXZ95905.1    AXZ96188.1    SPS73556.1    X71982    AY261361    KM111294    AJL34146.1    AY261360    KM111295    MH025916    MH025917    MH025918    MH025919    MH025920    AJL34311.1    AXB49362.1    AXB49536.1    AXB49708.1    AXB49879.1    AXB50052.1   

Proteomes

UP000000859    UP000130745    UP000101090    UP000108903    UP000110401    UP000142390    + More
UP000241813    UP000117635    UP000266411    UP000000858    UP000000624    UP000141072    UP000000860    UP000105860    UP000000861    UP000101566   

Pfam

PF01261   AP_endonuc_2

Interpro

IPR001719   AP_endonuc_2
IPR018246   AP_endonuc_F2_Zn_BS
IPR013022   Xyl_isomerase-like_TIM-brl
IPR036237   Xyl_isomerase-like_sf

SUPFAM

SSF51658   SSF51658

CDD

cd00019  AP2Ec

ProteinModelPortal

P0C9C6   A9JLC6   D4I5F7   A9JM65   A0A0A1DYA6   A0A1C6ZY19   + More
P0C9C7   Q65202   E0WM83   A0A2X0RUG5   Q65246   A0A0C5B2A8   P0C9C8   A0A0C5B2R9  

PANTHER

PTHR21445  

PDB

2X7W     E-value=7.6628e-09,     Score=143