Strain

Poland_Pol16_20538_o9_2016-2017 (Region: Poland; Strain: Pol16_20538_o9; Date: 2016-2017)

Gene

dUTPase

Description

Annoated by sequence mapping, VOCs Family: dUTPase

Location

MG939584.1(+):166511-167008

GenBank Accession

CAN10230.1

Full name

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Alternative Name

dUTP pyrophosphatase

CDS

ATGGCAACAAATTTTTTTATTCAACCTATCACCGAAGAAGCTGAAGCATACTACCCACCTTCCGTGATAACGAATAAACGGAAGGACCTGGGGGTAGACGTATACTGTTGCTCCGACCTAGTGCTTCAACCTGGACTAAATATTGTTCGCCTGCATATTAAAGTAGCATGCGAACACATGGGCAAAAAATGCGGTTTTAAAATCATGGCGAGAAGCAGTATGTGCACCCATGAACGGCTGCTCATCCTTGCAAACGGAATTGGTTTAATAGACCCGGGTTATGTGGGCGAGCTCATGCTCAAGATCATTAATCTTGGCGACACCCCGGTCCAAATATGGGCCAAAGAATGTTTGGTGCAGTTGGTGGCCCAAGGTGACCATGTGCCTGACCATATCAACATCCTAAAAAGAAACCAAATATTTCCGCTGTTTGCGCCTACCCCAAGAGGCGAGGGTAGATTTGGGAGCACGGGCGAGGCCGGGATTATGAGAACTTAA

Protein

MATNFFIQPITEEAEAYYPPSVITNKRKDLGVDVYCCSDLVLQPGLNIVRLHIKVACEHMGKKCGFKIMARSSMCTHERLLILANGIGLIDPGYVGELMLKIINLGDTPVQIWAKECLVQLVAQGDHVPDHINILKRNQIFPLFAPTPRGEGRFGSTGEAGIMRTX

Function

The viral dUTPase may play a role in lowering the dUTP concentration in natural infections to minimize misincorporation of deoxyuridine into the viral DNA and ensure the fidelity of genome replication.

Catalytic Activity

dUTP + H2O = diphosphate + dUMP + H(+)

Cofactor

Mg(2+)

Biophysicochemical Properties

1 uM for dUTP

Subunit

Homotrimer.

Miscellaneous

Expressed early and late in the infection.

Similarity

Belongs to the dUTPase family.

Keywords

Complete proteome Host cytoplasm Hydrolase Magnesium Metal-binding Nucleotide metabolism Reference proteome

Feature

chain Deoxyuridine 5'-triphosphate nucleotidohydrolase

Uniprot

A9JLC2 D4I5F4 A9JM59 A0A0A1E394 Q65199 E0WM80 + More
A0A2X0SE53 P0C9C4 P0C9C3 A0A0C5BCD8 P0C9C5 A0A0C5BCV3 Q65243

Pubmed

18198370 25406173 26618713 11831707 10515998 21470447 + More
25645905 8021596

EMBL

AM712239 CAN10230.1 FN557520 CBH29230.1 AM712240 CAN10479.1 + More
KM262844 KM262845 KM102979 KP055815 KX354450 AIY22321.1 AIY22479.1 AJZ77148.1 AKO62811.1 AOO54500.1 U18466 FR682468 CBW46796.1 MG939583 MG939588 LS478113 AXZ95902.1 AXZ96185.1 SPS73553.1 AY261366 AY261363 KM111294 AJL34143.1 AY261360 KM111295 MH025916 MH025917 MH025918 MH025919 MH025920 AJL34308.1 AXB49359.1 AXB49533.1 AXB49705.1 AXB49876.1 AXB50049.1 X71982 AY261361

Proteomes

UP000130745 UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 + More
UP000241813 UP000266411 UP000000624 UP000141072 UP000000858 UP000000859 UP000105860 UP000000861 UP000101566 UP000000860

PRIDE

Q65199 P0C9C3 P0C9C5

Pfam

PF00692 dUTPase

Interpro

IPR029054   dUTPase-like
IPR008181   dUTPase_1
IPR036157   dUTPase-like_sf
IPR033704   dUTPase_trimeric

SUPFAM

SSF51283 SSF51283

Gene 3D

2.70.40.10

CDD

cd07557 trimeric_dUTPase

ProteinModelPortal

A9JLC2 D4I5F4 A9JM59 A0A0A1E394 Q65199 E0WM80 + More
A0A2X0SE53 P0C9C4 P0C9C3 A0A0C5BCD8 P0C9C5 A0A0C5BCV3 Q65243

PANTHER

PTHR11241

PDB

6KZ6 E-value=3.20943e-96, Score=893

Ontologies

KEGG

vg:22220359

GO

GO:0046080  P:dUTP metabolic process
GO:0004170  F:dUTP diphosphatase activity
GO:0046872  F:metal ion binding
GO:0030430  C:host cell cytoplasm

Subcellular Location

From MSLVP

Cytoplasm

From Uniprot

Host cytoplasm

Topology

Length:

166

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.27411

Exp number, first 60 AAs:

0.00379

Total prob of N-in:

0.08282

outside

1 - 166

Population Genetic Test Statistics

Pi

0.0297151

Theta

0.0386839

Tajima's D

-0.8736362

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62811.1
China_AnhuiXCGQ_2018	Protein	AYW34101.1
China_ASFV-SY18_2018	Protein	AKO62811.1
Poland_Pol16_20186_o7_2016-2017	Protein	AXZ95902.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10230.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10230.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10230.1
Poland_Pol17_03029_C201_2016-2017	Protein	CAN10230.1
Poland_Pol17_04461_C210_2016-2017	Protein	AXZ96185.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10230.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10230.1
Uganda_R8_2015	Protein	AXB49359.1
Uganda_R7_2015	Protein	AXB49533.1
Uganda_R25_2015	Protein	AXB49705.1
Uganda_N10_2015	Protein	AXB49876.1
Uganda_R35_2015	Protein	AXB50049.1
Estonia_2014	Protein	SPS73553.1
Italy_26544_OG10_2010	Protein	AJZ77148.1
Italy_47_Ss_2008_2008	Protein	AOO54500.1
Portugal_OURT_88.3_1988	Protein	CAN10479.1
Spain_E75_1975	Protein	CBH29230.1
Russia_Georgia_2007	Protein	CBW46796.1
Kenya_Tk1_2005	Protein	AJL34143.1
Kenya_Bus_2006	Protein	AJL34308.1
Russia_Odintsovo_2014	Protein	CAN10230.1
Portugal_L60_1960	Protein	AIY22321.1
Portugal_NHV_1968	Protein	AIY22479.1
Russia_Kashino_2013	Protein	CAN10230.1
Benin_1997	Protein Protein	CAN10230.1 CAN10230.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10230.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10230.1
Malawi_Lil_1983	Protein	CAN10230.1
Kenya_1950	Protein	CAN10230.1
Namibia_Warthog_1980	Protein	CAN10230.1
South_Africa_Warmbaths_1987	Protein	CAN10230.1
Malawi_Tengani_1962	Protein	CAN10230.1
China_HLJ_2018	Protein	QBH90618.1
China_LN_2018	Protein	QBH90803.1
China_wbBS01_2018	Protein	QDL88161.1
Ukraine_Kyiv_2014	Protein	QED21743.1
Belgium_Etalle_wb_2018	Protein	QED90573.1
Lithuania_LT14_2014	Protein	QEY87936.1
South_Africa_1985	Protein	QGM12942.1
South_Africa_2_2008	Protein	QGM12868.1
Zambia_LIV_1983	Protein	QGM12763.1

Sequence