Strain

Kenya_Bus_2006 (Region: Kenya; Strain: Ken06.Bus; Date: 2006)

Gene

BA71V-B318L

Description

Annotated in NCBI, similar to African swine fever virus E75 strain E75 of INSD accession FN557520

Location

KM111295.1(-):92059-93015

GenBank Accession

AJL34253.1

Full name

Trans-prenyltransferase

Alternative Name

(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase
Farnesyl diphosphate synthase
Farnesyltranstransferase
Geranyltranstransferase
Polyprenyl-diphosphate synthase

CDS

ATGTTGCATCTCATCTATATCTCCATCATTGTAGTTCTTATTATTATTTTAATATCCTATACGCGGAAACCTCAGTATTTTAGAATCACTGCTCCGCGTAGCGTGGCGCTTTTTCATGGAATCCATCCGCTAAACCCTAAAAATTATAAAACCTTTAGCGAAGAGTTTGAAACCATTTTAAATAATGCTATCGAAGATGGGGACTTTAAGGGGCAACTAACAGAGCCTTGCTCTTACGCCCTAAGGGGTGGGAAGTATATCCGCCCCATTATCCTGATGGAAATTGTTCGGGCCTGTCAGCTGCAACATTCCTTTGGGGCGCCCATTTATCCGGCAGAGGCCGCCCTAGCGGTGGAGTACTTTCACGTGGCTTCTTTGATTATCGACGATATGCCCTCCTTTGACAACGATGTTAAGCGGCGAAATAAAGATACAGTGTGGGCTCGCTTTGGCGTAGCTAAGGCACAGATGAGCGCCCTGGCGCTAACCATGCAAGGATTTCAAAACATTTGTCGGCAAGTGGACTGGATTAAGGAAAACTGCCCAAGGTTTCCCGATCCTAACCAACTGGGAGCGCTGCTGTGCACCTTTGTAAGCCACTCTCTAAACAGCGCAGGTTCCGGTCAGTTAGTAGATACTCCAGAAAAAACAATTCCTTTTTTTAAAATCGCGTTTATTATGGGCTGGGTTTTGGGCACAGGAACTATTGAAGACATTGGGGCTATTGAAAGGGCCGCCCATTGTTTTGGAAATGCCTTTCAGTTAGCGGACGACATTAAGGACCATGACACAGATACGGGCCGCAATTACGCCAAAATACATGGAAAACAAAAAACATTTGACGTTGTGGTGCAGTCCCTGCAAGAGTGCAAAAAAATTCTCCATGAAAAAAAAATATATACCTCTATATGGAATGAGATTTTTCAAAAGGTTATAAATGTTGCATTGGGGACCTAA

Protein

MLHLIYISIIVVLIIILISYTRKPQYFRITAPRSVALFHGIHPLNPKNYKTFSEEFETILNNAIEDGDFKGQLTEPCSYALRGGKYIRPIILMEIVRACQLQHSFGAPIYPAEAALAVEYFHVASLIIDDMPSFDNDVKRRNKDTVWARFGVAKAQMSALALTMQGFQNICRQVDWIKENCPRFPDPNQLGALLCTFVSHSLNSAGSGQLVDTPEKTIPFFKIAFIMGWVLGTGTIEDIGAIERAAHCFGNAFQLADDIKDHDTDTGRNYAKIHGKQKTFDVVVQSLQECKKILHEKKIYTSIWNEIFQKVINVALGT

Function

Trans-prenyltransferase that catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with different allylic diphosphates, such as dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP), farnesyl diphosphate being the best allylic substrate.

Catalytic Activity

dimethylallyl diphosphate + isopentenyl diphosphate = (2E)-geranyl diphosphate + diphosphate
(2E)-geranyl diphosphate + isopentenyl diphosphate = (2E,6E)-farnesyl diphosphate + diphosphate
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = all-trans-geranylgeranyl diphosphate + diphosphate
all-trans-geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate

Cofactor

Mg(2+)

Similarity

Belongs to the FPP/GGPP synthase family. Asfivirus trans-prenyltransferase subfamily.

Keywords

Complete proteome Host endoplasmic reticulum Host membrane Isoprene biosynthesis Late protein Magnesium Membrane Metal-binding Transferase Transmembrane Transmembrane helix Reference proteome

Feature

chain Trans-prenyltransferase

Uniprot

A0A0C5BCP6 A0A2Z5DF16 A0A0C5BC85 P0C9E2 E0WML3 A0A2X0RUY3 + More
P0C9E1 A9JL29 D4I5R3 A9JLU7 A0A0A1E0R4 Q65164 P0C9E0 Q8V9T7

Pubmed

25645905 21470447 18198370 25406173 26618713 11831707 + More
9083080

EMBL

KM111295 AJL34253.1 MH025916 MH025917 MH025918 MH025919 + More
MH025920 AXB49303.1 AXB49477.1 AXB49649.1 AXB49820.1 AXB49993.1 KM111294 AJL34088.1 AY261360 FR682468 CBW46741.1 LS478113 SPS73498.1 AY261366 AM712239 CAN10174.1 FN557520 CBH29176.1 AM712240 CAN10424.1 KM262844 KM262845 KM102979 KP055815 KX354450 AIY22266.1 AIY22424.1 AJZ77099.1 AKO62756.1 AOO54414.1 U18466 AY261363 L00966 AY261361

Proteomes

UP000101566 UP000105860 UP000000861 UP000141072 UP000000858 UP000130745 + More
UP000101090 UP000108903 UP000110401 UP000117635 UP000142390 UP000241813 UP000266411 UP000000624 UP000000859 UP000000860

PRIDE

Q65164

Pfam

PF00348 polyprenyl_synt

Interpro

IPR000092 Polyprenyl_synt
IPR008949 Isoprenoid_synthase_dom_sf

SUPFAM

SSF48576 SSF48576

Gene 3D

1.10.600.10

ProteinModelPortal

A0A0C5BCP6 A0A2Z5DF16 A0A0C5BC85 P0C9E2 E0WML3 A0A2X0RUY3 + More
P0C9E1 A9JL29 D4I5R3 A9JLU7 A0A0A1E0R4 Q65164 P0C9E0 Q8V9T7

PDB

2J1P E-value=2.40447e-13, Score=182

Ontologies

KEGG

vg:22220304

GO

GO:0016021  C:integral component of membrane
GO:0008299  P:isoprenoid biosynthetic process
GO:0044165  C:host cell endoplasmic reticulum
GO:0033384  P:geranyl diphosphate biosynthetic process
GO:0046872  F:metal ion binding
GO:0004337  F:geranyltranstransferase activity
GO:0045337  P:farnesyl diphosphate biosynthetic process
GO:0004161  F:dimethylallyltranstransferase activity
GO:0033386  P:geranylgeranyl diphosphate biosynthetic process
GO:0033644  C:host cell membrane
GO:0004311  F:farnesyltranstransferase activity
GO:0016740  F:transferase activity

Subcellular Location

From MSLVP

Host nucleus. Host cytoplasm.

From Uniprot

Host endoplasmic reticulum
Host membrane

Topology

Length:

318

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

21.68384

Exp number, first 60 AAs:

18.80481

Total prob of N-in:

0.60105

POSSIBLE N-term signal

sequence

inside

1 - 1

TMhelix

2 - 21

outside

22 - 318

Population Genetic Test Statistics

Pi

0.0324036

Theta

0.0386839

Tajima's D

-0.8568248

CLR

0

Interpretation

No evidence of Selection

Multiple alignment of Orthologues

Gene Tree

Orthologous in Strains

Strain	Status	Gene
Spain_BA71_1971	Protein	AKO62756.1
China_AnhuiXCGQ_2018	Protein	AYW34046.1
China_ASFV-SY18_2018	Protein	AKO62756.1
Poland_Pol16_20186_o7_2016-2017	Protein Protein	CAN10174.1 AXZ95849.1
Poland_Pol16_20538_o9_2016-2017	Protein	CAN10174.1
Poland_Pol16_20540_o10_2016-2017	Protein	CAN10174.1
Poland_Pol16_29413_o23_2016-2017	Protein	CAN10174.1
Poland_Pol17_03029_C201_2016-2017	Protein Protein	CAN10174.1 AXZ96037.1
Poland_Pol17_04461_C210_2016-2017	Protein Protein	CAN10174.1 AXZ96132.1
Poland_Pol17_05838_C220_2016-2017	Protein	CAN10174.1
Poland_ASFV_POL_Podlaskie_2015	Protein	CAN10174.1
Uganda_R8_2015	Protein	AXB49303.1
Uganda_R7_2015	Protein	AXB49477.1
Uganda_R25_2015	Protein	AXB49649.1
Uganda_N10_2015	Protein	AXB49820.1
Uganda_R35_2015	Protein	AXB49993.1
Estonia_2014	Protein	SPS73498.1
Italy_26544_OG10_2010	Protein	AJZ77099.1
Italy_47_Ss_2008_2008	Protein	AOO54414.1
Portugal_OURT_88.3_1988	Protein	CAN10424.1
Spain_E75_1975	Protein	CBH29176.1
Russia_Georgia_2007	Protein	CBW46741.1
Kenya_Tk1_2005	Protein	AJL34088.1
Kenya_Bus_2006	Protein	AJL34253.1
Russia_Odintsovo_2014	Protein	CAN10174.1
Portugal_L60_1960	Protein	AIY22266.1
Portugal_NHV_1968	Protein	AIY22424.1
Russia_Kashino_2013	Protein	CAN10174.1
Benin_1997	Protein Protein	CAN10174.1 CAN10174.1
South_Africa_KNP_Pretorisuskop_1996	Protein	CAN10174.1
South_Africa_MGR_Mkuzi_1979	Protein	CAN10174.1
Malawi_Lil_1983	Protein	CAN10174.1
Kenya_1950	Protein	CAN10174.1
Namibia_Warthog_1980	Protein	CAN10174.1
South_Africa_Warmbaths_1987	Protein	CAN10174.1
Malawi_Tengani_1962	Protein	CAN10174.1
China_HLJ_2018	Protein	QBH90563.1
China_LN_2018	Protein	QBH90748.1
China_wbBS01_2018	Protein	QDL88106.1
Ukraine_Kyiv_2014	Protein	QED21652.1
Belgium_Etalle_wb_2018	Protein	QED90518.1
Lithuania_LT14_2014	Protein	QEY87881.1
South_Africa_1985	Protein	CAN10174.1
South_Africa_2_2008	Protein	CAN10174.1
Zambia_LIV_1983	Protein	CAN10174.1

Sequence